222 results about "Eosin" patented technology

Apparatus, methods, and computer-readable media are provided for segmentation, processing (e.g., preprocessing and / or postprocessing), and / or feature extraction from tissue images such as, for example, images of nuclei and / or cytoplasm. Tissue images processed by various embodiments described herein may be generated by Hematoxylin and Eosin (H&E) staining, immunofluorescence (IF) detection, immunohistochemistry (IHC), similar and / or related staining processes, and / or other processes. Predictive features described herein may be provided for use in, for example, one or more predictive models for treating, diagnosing, and / or predicting the occurrence (e.g., recurrence) of one or more medical conditions such as, for example, cancer or other types of disease.

The invention discloses a manufacturing method of paraffin sections of zostera marina embryo, comprising the following steps: (1) material drawing and fixation: zostera marina seeds are taken and the fruit skin is stripped, and after endosperm is removed, an integral zostera marina embryo is soaked in FAA (free amino acids) fixing solution and is fixed for more than 48 hours; (2) dehydration: the zostera marina embryo is taken out from the fixing solution and is soaked in alcohol for dehydration; (3) waxing and embedding: paraffin is added gradually in a vessel containing the zostera marina embryo and dimethylbenzene, and conventional paraffin embedding is carried out to obtain the wax blocks containing the zostera marina embryo; (4) slicing, spreading and drying: the wax blocks coated with the zostera marina embryo is fixed on a wheel rotation type slicing machine for carrying out continuous slicing of the paraffin, so as to obtain wax bands and enable the wax bands to be attached to an object slide; (5) dewaxing, rehydration and dyeing of hematoxylin dye solution; (6) slicing, dehydration and dyeing of eosin and alcohol; and (7) transparence and mounting are carried out to manufacture a permanent section. In the manufacturing method disclosed by the invention, the paraffin sections of the zostera marina embryo, which have clear dyeing and an integral texture structure, can be obtained.

The invention provides a hematoxylin staining solution which comprises the following components of hematoxylin, aluminium salt, an oxidizing agent, benzalkonium chloride, alcohol, weak acid and water. The invention further provides a preparation method of the hematoxylin staining solution, a HE (hematoxylin eosin) staining solution containing the hematoxylin staining solution, and a staining method. When the hematoxylin staining solution is used to stain a section, the background of the section is clear, the section is well arranged, the staining effect is excellent, the section is easy to read, the color of the section does not fade easily, and the section can be stored conveniently for a long period of time.

A pathological diagnosis support device, a pathological diagnosis support program, a pathological diagnosis support method, and a pathological diagnosis support system extract a pathological tissue for diagnosis from a pathological image and diagnose the pathological tissue. A tissue collected in a pathological inspection is stained using, for example, hematoxylin and eosin. In consideration of the state of the tissue in which a cell nucleus and its peripheral constituent items are stained in respective colors unique thereto, subimages such as a cell nucleus, a pore, cytoplasm, interstitium are extracted from the pathological image, and color information of the cell nucleus is also extracted. The subimages and the color information are stored as feature candidates so that presence or absence of a tumor and benignity or malignity of the tumor are determined.

A green making technology of animal tissue paraffin section disclosed in the invention aims at providing a technique of making a paraffin section of animal tissue applied to the agricultural university medicine and other majors, characterized by that the type of chemical reagents that are used is limited, the chemical reagents have no toxicity, and dehydrate the tissue thoroughly, accelerate making sections but not lead to excessive contraction and increasement of hardness of the tissue, and substitute dimethyl benzene used in the traditional making technology of the paraffin section which isharmful to human body, so as to eliminate the occupational diseases and environmental pollution caused by dimethyl benzene. The making technology comprises the following steps: carrying out fixation with a fixative solution (100ml of the fixative solution comprises 91 ml 85% alcohol, 4 ml methanol and 5 ml glacial acetic acid ), dehydrating the tissue with graded ethanol, then putting the fixative and dehydrated tissue in a mixed transparent reagent (comprising 14wt% of analytically pour n-butanol, 29wt% of acetone and 57wt% of absolute alcohol ) for standing for 3 hours, then carrying out normal waxing and embedding, using analytically pour turpentine (60 DEG C) for section staining, carrying out dewaxing twice, carrying out hydration under normal temperature, using haematoxylin for staining nuclei, decoloration and bluing, and using eosin for staining, dehydrating until get absolute alcohol, then carrying out air drying, and directly using the gum diluted by turpentine to sealing the section.

The invention provides a hematoxylin eosin staining solution, comprising a hematoxylin staining solution and an eosin staining solution, wherein the hematoxylin staining solution comprises hematoxylin, an alumium salt, an oxidizing agent, benzalkonium chloride, an alcohol, a weak acid and water; the eosin staining solution comprises eosin, Biebrich scarlet, flame red B, water and ethanol. The invention also provides a preparation method of the hematoxylin eosin staining solution, and a dyeing method thereof. When the hematoxylin eosin staining solution provided by the invention is used for dyeing slices, the effects of clear background, distinct gradation, good dyeing effect, easy slice reading, not easy fading and convenience for long-term storage can be achieved.

A method for qualifying a specimen prepared on one or more hematoxylin and eosin (H&E) slides by assessing an expected yield of nucleic acids for tumor cells and providing associated unstained slides for subsequent nucleic acid analysis is provided.

A biological substance detection method for detecting a biological substance specifically in a pathological specimen, comprising a step of immunologically staining the pathological specimen using a fluorescent label, a step of staining the pathological specimen with a staining reagent for morphology observation purposes (eosin) to observe the morphology of the pathological specimen, a step of irradiating the stained pathological specimen, with excited light to cause the emission of a fluorescent and detecting the biological substance in the pathological specimen. In the step of immunologically staining the pathological specimen, a special fluorescent particle for which the excitation wavelength appears in a region that is different from the excitation wavelength region of eosin is used as the fluorescent label.

The invention relates to a method for visible light catalytic synthesis of a 3-sulfuryl spiro-trienone compound. The method comprises dissolving N-aryl propiolamide and sulfinic acid as raw materials in an acetonitrile / water mixed solvent, adding water soluble eosin as a photocatalyst into the solution, carrying out irradiation on the mixed solution under a 3W blue LED visible light lamp for a reaction at the room temperature for 6-24h, and after the reaction, separating and purifying the crude product to obtain the 3-sulfuryl spiro-trienone compound. The method utilizes a visible light energy source, a simple and easy available sulfinic acid as a sulfone source, cheap water soluble eosin as a photocatalyst and air as an oxidant, realizes synthesis of the 3-sulfuryl spiro-trienone compound at the room temperature, greatly reduces the energy consumption, effectively prevents the use of metal catalysts and peroxide oxidants and has good reaction safety.

A method for unsupervised classification of histological images of prostatic tissue includes providing histological image data obtained from a slide simultaneously co-stained with NIR fluorescent and Hematoxylin-and-Eosin (H&E) stains, segmenting prostate gland units in the image data, forming feature vectors by computing discriminating attributes of the segmented gland units, and using the feature vectors to train a multi-class classifier, where the classifier classifies prostatic tissue into benign, prostatic intraepithelial neoplasia (PIN), and Gleason scale adenocarcinoma grades 1 to 5 categories.

The invention provides a catalyst for generating hydrogen by visible light photocatalytic reduction of water, and a preparation method thereof, wherein each 100mg of the catalysts contain 10-60mg of eosin-Y, 35-85mg of carbon nano tubes and 1-10mg of oxides. With the technical scheme of the invention, the eosin is used as a sensitizing agent, the carbon nano tubes are used as carriers and photogenerated electron channels, the oxides, such as copper oxide, are used as co-catalysts, so that hydrogen generation by visible light photocatalytic reduction of water is implemented. The catalyst has higher hydrogen generation activity under irradiation of the visible light, and can be used for generating hydrogen by visible light photocatalytic reduction of water. And the oxides substitute Pt to be used as the co-catalyst, thereby implementing non-platinum property of the catalyst.

Automated staining equipment that can mix reagents is used to spray a Romanowsky stain onto slide mounted specimens which are then briefly centrifuged. The centrifugation step removes excess stain leaving only a thin film. Depending on the time of the centrifugation step, most of the organic solvent and part of the water in the stain are evaporated by airflow through the equipment. This greatly accelerates the staining reaction and preserves water soluble structures such as the granules in basophilic leukocytes. For optimal performance, this staining procedure requires a thiazin-eosin stain with about 90% to about 40% organic solvent, such as methanol, and only about 10% to about 60% water. This is a unique staining reagent in Romanowsky staining.

The invention discloses a preparation method and an application of a tissue slice for observing temporal-spatial distribution of early embryo development in vivo. The preparation method comprises the following steps that 4% paraformaldehyde fixing, upward gradient ethanol dehydration, wax dipping, embedding, serial section, baking, dewaxing and downward gradient ethanol rehydration are performed in sequence on oviducts or uterine tissues which contain mice embryos in every period, and finally, after haematoxylin-eosin staining is performed on the tissues in the slice, neutral gum is used for sealing the slice, or after immunofluorescence histochemical staining is performed on the slice, a fluorescence resistant quenching sealing agent is used for sealing the slice. The tissue slice disclosed by the invention can be used for manufacturing a map of early mice embryo development and detecting the expression of Crb3 in the mice embryos in every period of development in vivo. The preparation method has the advantages that positions of all organs in the embryos can be relatively fixed, so that the position change of embryo cells in a genital tract and the continuity of embryo development can be conveniently observed, the structure is clear, and the tissue slice is convenient to store.

The invention provides a hematoxylin-eosin mixed staining solution, which is characterized by comprising the following components: hematoxylin, an oxidant, a mordant, eosin, alcohol, weak acid, a preservative and water. The novel staining solution is prepared by mixing hematoxylin and eosin together; and cell and tissue samples only need one-time staining for staining both the hematoxylin and eosin. The present invention also provides a preparation method and a staining method of the hematoxylin-eosin mixed staining solution, and solves the problems of complicated operation, time consumption, false blue, unclear hierarchy and short retention time on the traditional hematoxylin-eosin staining.

The invention discloses a hematoxylin-eosin staining pathological image segmentation method based on unsupervised deep learning, and the method comprises the steps: carrying out the preprocessing andfeature extraction after an HE staining pathological image is obtained, dividing pixels into five types through Kmeans clustering, using a given training sample full-automatic grabbing strategy for carrying out traversal grabbing on category label images obtained through clustering to obtain reliable training samples, then using a training set for training a semantic segmentation model Unet, and designing different training strategies before, in the middle and after training; segmenting the to-be-segmented image into the size conforming to model input in an overlapping manner, putting the to-be-segmented image into the trained model to obtain a prediction result, and splicing the prediction result to obtain a segmentation result of the cell nucleus foreground; and finally, carrying out accurate kernel boundary segmentation on the cell nucleus part by using a hybrid watershed segmentation method to obtain a complete segmentation result. According to the method, the high efficiency of unsupervised learning and the high precision of deep learning are organically combined, and the precision and the efficiency of cell nucleus region segmentation in a pathological image segmentation taskare remarkably improved.

The invention provides a paraffin sectioning method for peanut seeds. The method includes the steps of 1) material selection; 2) fixation by glutaraldehyde 5%-10% in concentration; 3) dehydration: dehydrating by ethanol 80% in concentration for 50min, ethanol 95% in concentration for 45min and ethanol 100% in concentration for 45min and repeating each gradient for one time; 4) transparency: treating by ethanol 100% in concentration for 20-30min, and treating by dimethylbenzene for 15-25min twice, wherein the volume ratio of the dimethylbenzene to the ethanol is 1:1; 5) paraffin permeation by a direct paraffin permeation method, embedding and sectioning; and 6) deparaffinage, rehydration, hematoxylin-eosin redyeing, dehydration, transparency and sealing. The method is capable of well fixing cellular tissue of the peanut seeds, complete and continuous in sectioning, free of cavities, simple to operate, applicable to all peanut varieties and convenient to popularize and apply, and section thickness can reach 7 micrometres.

The present invention provide for solutions of a defined composition useful in a staining protocol, such as a hematoxylin and eosin staining protocol, when used at certain points of the staining protocol. The formulations of these defined solutions are such that carry-over of the solutions will not negatively impact, or preferably, will stabilize or favorably modify staining reagent solutions coming in contact with the solutions. In certain embodiments of the invention, solutions are buffered to maintain a specific pH that when carried-over—such as carried-over into hematoxylin—will not significantly influence the pH of the next staining reagent.

The invention discloses a method for testing a curative effect of a Chinese medicine Zhibai Dihuang pill based on application of metabonomics. The method comprises the following steps: dividing experimental animals into a control group, a diabetic nephropathy model group and a diabetic nephropathy administration group, injecting the experimental animals and collecting tissue samples of urine, serum and kidney; fixing the renal tissues of the three groups of rats through 10% neutral formalin, carrying out paraffin embedding, slicing and flaking the renal tissues, staining the renal tissues through routine hematoxylin-eosin and observing the pathological changes of the renal tissues under an optical microscope; measuring the chemical indexes and detecting the histopathology of the serum samples and the urine samples; preparing tissue samples of urine, serum and kidney, and obtaining data of a 1H NMR (Nuclear Magnetic Resonance) spectrum; carrying out NMR data processing and pattern recognition analysis through a statistical method; and comparing and analyzing important physiological and biochemical indexes of different groups of rats. The method disclosed by the invention can be used for obtaining the information of a large amount of endogenous metabolites of an organism through a metabonomics technology and screening metabolite changes caused by effective ingredients in the Zhibai Dihuang pill.

The invention discloses an eosin liposome and making method, which comprises the following parts: 18-22mg eosin, 505-509mg lecithin, 90-95mg cholesterol, 73-77mg sodium deoxycholate, 505-509mg mannitol, 505-509mg lactose and 18-22mL phosphate buffer with pH value at 5.5-6.5. the making method comprises the following steps: (1) adding eosin, sodium deoxycholate, lecithin and cholesterol into chloroform; (2) decompressing; evaporating chloroform; (3) adding lactose and mannitol into phosphate buffer; (4) emulsifying phosphate buffer without fat film; (5) freezing; drying to obtain the product.

The invention discloses a deep learning-based intelligent detection method for nuclear division images in gastrointestinal stromal tumor. The method comprises the following steps: preprocessing an obtained hematoxylin-eosin staining pathological image; taking EfficientDet-D0 as a deep learning detection model, and carrying out training; using U-Net as a deep learning segmentation model, and training the deep learning segmentation model; constructing a deep learning classification model; training the deep learning classification model; detecting the hematoxylin-eosin staining pathological imageof the testee by using the trained deep learning detection model; segmenting the pathological image by using a deep learning segmentation model, and detecting the segmented result; and comparing thenuclear division images detection result based on the deep learning detection model with the nuclear division images detection result based on the deep learning segmentation model to obtain a final classification result. According to the invention, the input hematoxylin-eosin staining image is analyzed, and the number of nuclear division images is detected, so that the judgment on the risk degreeof gastrointestinal stromal tumor is realized.

The invention provides an eosin staining solution comprising the components of eosin, Biebrich scarlet, flame red, ethanol and water. The invention also provides a preparation method of the eosin staining solution, a HE staining solution containing the eosin staining solution and a staining method of the eosin staining solution. When the eosin staining solution provided by the invention is used for staining slices, the background is clear, the gradation is distinct, the staining effect is good, and the slices can be read easily, cannot fade easily and are convenient to store for a long time.

The invention discloses a papanicolaou staining kit and a staining method thereof. The kit comprises hematoxylin stain, a back-to-blue liquid, eosin stain and a cleaning solution. The eosin stain is prepared from the following substances in concentration: 600-900ml / L ethanol, 100-400ml / L methanol, 1-4g / L eosin Y, 1-4g / L phosphotungstic acid, 0.01-2g / L fast green and 1-10ml / L weak acid; and the cleaning solution is prepared by mixing solvent oil, absolute ethyl alcohol and propylene glycol methyl ether acetate according to a volume ratio of (10-350):(600-950):(10-200). The papanicolaou stainingkit disclosed by the invention is rapid in staining, the stained colors have clear differences, and the diagnosis efficiency is effectively improved.

The invention discloses a preparation method of a hyriopsis cumingii pallium tissue slice. The preparation method comprises the following steps of: carrying out fixation, upstream gradient ethanol dehydration, vifrification, waxdip, embedding, sectioning, baking, dewaxing and downstream gradient ethanol rehydration on pallium tissue on cumingii pallium, and mounting on the slice tissue by adopting neutral resins after carrying out hematoxylin-eosin on the slice tissue. The tissue slice prepared by the invention can be used for observing the histology characteristic of the pallium, and detecting expression of related genes forming the shell on the positions of the hyriopsis cumingii pallium; the method provided by the invention can guarantee the relative stationarity between positions of each layers of tissues of the pallium and the cell; and compared with the common preparation method of the tissue slice, the preparation method provided by the invention has the advantages that time is saved, the problem of tissue fracture of the tissue slice for the pallium manufactured by the common method is overcome, and the repeatability is better.

A method of preparing cell samples for viable cell number quantification with a fluorescence digital imaging microscopy system employing digital thresholding technique. The cell sample is stained with a first, fluorescent dye and treated with a second dye that is able to quench the fluorescence of the first dye. The fluorescent dye accumulates in viable cells only and is used to stain the viable cells. The second dye is excluded from viable cells but enters non-viable cells, thereby quenching the background fluorescence in non-viable cells and the medium. Two examples of dye combinations are described: fluorescein diacetate used as the fluorescent dye with eosin Y as the quenching dye; and calcein-AM used as the fluorescent dye with trypan blue as the quenching dye. By reducing the background fluorescence, the dynamic range and accuracy of viable cell number measurements are enhanced. In low viability cultures treated with fluorescein diacetate, background fluorescence completely masked viable cells, but digital thresholding and eosin treatment dramatically reduced background fluorescence, producing a linear response over 4 logs of viable cell density.

The invention provides a PET / CT (Positron Emission Tomography / Computed Tomography) macroscopical digital information and pathological microscopic information matching method. A postoperative sample is reduced into an in-vivo state and is soaked into formalin; a cross section is formed between the tracer highest-ingestion layer surface in a PET / CT image and an adjacent anatomic landmark; the sample is cut apart from the middle in the cross section direction, and is soaked into the formalin; HE (Hematoxylin-Eosin) staining pathological sections are manufactured; a paraffin block region of an immune tissue chemical staining region in each HE staining pathological section is determined; the selected paraffin block region is fixed, and the paraffin section cutting is performed; and the parameter of the tracer high-ingestion layer surface in each layer of PET / CT image and the corresponding layer immunohistochemistry expression are analyzed. The PET / CT image information and immunohistochemistry information matching accuracy is improved; a tracer high-ingestion region and a histology region are accurately registered; and after the matching, the accuracy of the PET / CT on the detection of the in-vivo oncobiology information can be verified.

The present invention relates to methods of visualizing targets in histological samples, e.g. biopsy samples, wherein the methods comprise staining of the sample with (i) one or more target specific immunochemical stains, and (ii) a histological stain for specific tissue components e.g. iron, mucins glycogen, amyloid, nucleic acids, etc., e.g. hematoxilyn and / or eosin stains or the like, that is used to enhance contrast in the microscopic image of a tissue sample, highlight morphologic structures in the sample for viewing, define and examine tissues, cell populations, or organelles within individual cells. Methods may further comprise evaluation of expression of one or more targets in the sample. The disclosed methods are useful for medical diagnostics.

The present invention provides a variable color transparent lip balm, consisting of following components by weight percent: 4.0 to 5.0% by weight of dibutyl lauroyl glutamide, 2.0 to 3.0% by weight of polyamide-3, 15.0 to 20.0% by weight of bis-stearyl ethylenediamine / neopentyl glycol / stearyl hydrogenated dimer dilinoleate copolymer, 46.65 to 49.60% by weight of C12-15 alkyl benzoate, 1.0 to 3.0% by weight of heptyl undecylenate, 9.0 to 11.0% by weight of C10-30 cholesterol / lanosterol esters, 10.0 to 15.0% by weight of hydrogenated C6-20 polyolefin, 1.5 to 2.5% by weight of dextrin isostearate, 0.05 to 0.1% by weight of eosin yellowish and 0 to 0.8% by weight of excipient. The transparent lip balm of the present invention has good transparency, and excellent variable color effect.

The invention discloses an eosin constantan methanesulfonic acid salt and component and making method, wherein the eosin constantan methanesulfonic acid salt possesses chemical structural formula (n=1-2), which possesses excellent solubility and stability; the component of eosin constantan methanesulfonic acid salt consists of eosin constantan methanesulfonic acid salt and acceptable carrier, which can prepare kinds of liquid, freeze-drying and film or skin give drug.

The invention discloses an experimental method of effects of DNA oxidative damage and bone cell apoptosis on avascular necrosis, the method comprises the following steps: experimental grouping and establishment of an animal model, preparation of specimens, histomorphology observation under a light microscope, observation by a transmission electron microscope, bone cell apoptosis situation detection by TUNEL (terminal dexynucleotidyl transferase(TdT)-mediated dUTP nick end labeling) method, bone marrow hematopoietic cell DNA oxidative damage detection by monoclonal antibody N45.1 immunohistochemistry method, and statistical analysis. According to the method, experiment animals are respectively killed in the second week and the fourth week after the last time of administration, and five animals are killed for each time in each group; bilateral femoral heads are taken for conventional fixed decalcification for stand-by testing, the number of vacant bone lacunas is counted by HE (hematoxylin-eosin) staining, the bone cell morphological change is observed by the electron microscope, the bone cell apoptosis situation is detected by the TUNEL method, the bone marrow hematopoietic cell DNA oxidative damage is detected by the immunohistochemistry method, and the relationship between SANFH ((steroid-induced avascular necrosis of femoral head)) and the bone marrow hematopoietic cell DNA oxidative damage and the bone cell apoptosis is explored, and provides new ideas for the further study of pathogenesis of the steroid induced avascular necrosis of femoral head.

The invention relates to the technique of protein negative staining detection, in particular to an application of an eosin and a derivative of the eosin in protein negative staining detection. A method using the eosin to perform protein negative staining detection is further provided, which comprises the steps of fixing a gel of a protein sample after electrophoresis in a stationary liquid, washing with deionized water, performing equilibrium in a methanol solution, staining and developing. The application of the eosin and the derivative of the eosin in protein detection have the advantages of being high in sensitivity, simple and quick in operation, good in reproducibility, linear relation and mass spectrum compatibility, safe in usage, low in cost, applicable to the research of high throughput proteomics and the like.