Manufacturing method of paraffin sections of zostera marina embryo

Abstract

The invention discloses a manufacturing method of paraffin sections of zostera marina embryo, comprising the following steps: (1) material drawing and fixation: zostera marina seeds are taken and the fruit skin is stripped, and after endosperm is removed, an integral zostera marina embryo is soaked in FAA (free amino acids) fixing solution and is fixed for more than 48 hours; (2) dehydration: the zostera marina embryo is taken out from the fixing solution and is soaked in alcohol for dehydration; (3) waxing and embedding: paraffin is added gradually in a vessel containing the zostera marina embryo and dimethylbenzene, and conventional paraffin embedding is carried out to obtain the wax blocks containing the zostera marina embryo; (4) slicing, spreading and drying: the wax blocks coated with the zostera marina embryo is fixed on a wheel rotation type slicing machine for carrying out continuous slicing of the paraffin, so as to obtain wax bands and enable the wax bands to be attached to an object slide; (5) dewaxing, rehydration and dyeing of hematoxylin dye solution; (6) slicing, dehydration and dyeing of eosin and alcohol; and (7) transparence and mounting are carried out to manufacture a permanent section. In the manufacturing method disclosed by the invention, the paraffin sections of the zostera marina embryo, which have clear dyeing and an integral texture structure, can be obtained.

Description

technical field [0001] The invention relates to a method for making a paraffin section, in particular to a method for making a paraffin section of an algae embryo. Background technique [0002] Zostera marina L., commonly known as kelp grass, is a marine higher seed plant, belonging to the phylum Angiospermae, monocotyledoneae (Monocotyledoneae, or Liliopsida), order Alismales, eye Potamogetonaceae, genus Zostera L. Zostera can live submerged in the ocean, can perform photosynthesis like higher plants on land, and use seeds to reproduce offspring. In order to adapt to the marine submerged environment, the internal tissue structure of Z. eosinophila seed plants is quite different from that of terrestrial seed plants. For example, there are strong Na and K pump structures on the cell membrane, the epidermal cells are closely arranged, and the cell walls are thicker, so water is not easily absorbed. Complete replacement, cells with high osmotic pressure, high water content and...

AI Technical Summary

Problems solved by technology

Because of the above-mentioned differences between the seeds of Z. echinophyllum and terrestrial seeds, and the existing paraffin section method is mainly used for terrestrial plants, the paraffin sections of Z. echinacea embryo obtained by using the existing paraffin section method are not clearly stained and the tissue structure is incomplete.

Tracing it to its cause, the fixed time of the paraffin section of the algae embryo paraffin that existing paraffin section method is made is short, and tissue is deformed easily; Brittle and hard, unable to cut complete, clear paraffin sections

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