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Hematoxylin staining solution and HE (hematoxylin eosin) staining solution containing hematoxylin staining solution

A technology of staining solution and hematoxylin, which is applied in the preparation of organic dyes and test samples, etc., can solve the problems of easy generation of metal film and dye precipitation, blurred background of tissue sections or cell smears, contamination of sections, etc., and achieve inhibition of mold, etc. Production of a variety of bacteria, high-efficiency broad-spectrum bactericidal ability, and the effect of preventing contamination of slices

Inactive Publication Date: 2014-04-02
JIANGSU INST OF NUCLEAR MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] After long-term use, people found that the existing hematoxylin staining solution also has the following defects: 1) It is easy to produce metal film and dye precipitation, thereby contaminating the slices, and in the process of making slices, the above-mentioned stains on the slices are difficult to remove, resulting in Inhomogeneous staining, or even unreadable slides, need to be re-made; 2) False blue staining is prone to occur during the staining stage, and part of the cytoplasm is also stained blue, which affects the accuracy of subsequent observation results
[0013] The existing HE staining solution, that is, the hematoxylin and eosin staining solution, due to the metal film and dye precipitation produced by the hematoxylin staining solution, will cause uneven staining and prone to false blue staining, which in turn will cause tissue staining after hematoxylin and eosin staining. Blurred background of sections or cell smears, difficult to read

Method used

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  • Hematoxylin staining solution and HE (hematoxylin eosin) staining solution containing hematoxylin staining solution
  • Hematoxylin staining solution and HE (hematoxylin eosin) staining solution containing hematoxylin staining solution
  • Hematoxylin staining solution and HE (hematoxylin eosin) staining solution containing hematoxylin staining solution

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Embodiment 1

[0063] The hematoxylin staining solution of the present embodiment includes the following components in every 100ml of the hematoxylin staining solution: hematoxylin 1g; aluminum salt: aluminum ammonium sulfate 10g; oxidizing agent: red mercuric oxide 0.25g; benzalkonium chloride 0.1g; alcohol: ethanol 10ml; weak acid: glacial acetic acid 2.1g; the balance is deionized water.

[0064] Its preparation method is as follows:

[0065] 1) Preparation of hematoxylin staining solution:

[0066] a. Take alcohol and hematoxylin according to the selected ratio, add the hematoxylin into the alcohol, dissolve to obtain hematoxylin alcohol solution, and set aside;

[0067] b. Take water and aluminum salt in a selected ratio, completely dissolve the aluminum salt in water, add the hematoxylin alcohol solution obtained in step a, to obtain a mixed solution;

[0068] c. Take oxidant, benzalkonium chloride and weak acid according to the selected ratio, stir the oxidant into the mixed solutio...

Embodiment 2

[0078] The hematoxylin staining solution of the present embodiment includes the following components in every 100 ml of the hematoxylin staining solution: 0.3 g of hematoxylin; aluminum salt: 10 g of potassium aluminum sulfate; oxidizing agent: 0.03 g of sodium iodate; 0.525 g of benzalkonium chloride; alcohol: Ethylene glycol 5ml; weak acid: salicylic acid 0.03g; the balance is distilled water.

[0079] Its preparation method is as follows:

[0080] a. Take alcohol and hematoxylin according to the selected ratio, add the hematoxylin into the alcohol, dissolve to obtain hematoxylin alcohol solution, and set aside;

[0081] b. Take water and aluminum salt in a selected ratio, completely dissolve the aluminum salt in water, add the hematoxylin alcohol solution obtained in step a, to obtain a mixed solution;

[0082] c. Take oxidant, benzalkonium chloride and weak acid according to the selected ratio, stir the oxidant into the mixed solution obtained in step b, heat the solution...

Embodiment 3

[0092]The hematoxylin staining solution of the present embodiment includes the following components in every 100ml of the hematoxylin staining solution: 0.65 g of hematoxylin; aluminum salt: 6.25 g of aluminum sulfate; oxidizing agent: 0.11 g of potassium permanganate; 0.05 g of benzalkonium chloride; : Glycerol 32.5ml; weak acid: 0.2g citric acid; the balance is deionized water.

[0093] Its preparation method is the same as that in Example 2.

[0094] The HE staining solution of this embodiment includes the hematoxylin staining solution prepared above, and also includes the eosin staining solution.

[0095] The eosin staining solution includes the following components in every 100ml of the eosin staining solution: water-soluble eosin Y0.3g; bibreci scarlet 0.05g; flamingo red B0.15g; benzalkonium chloride 0.525g; ethanol 60ml; the balance is distilled water.

[0096] Its preparation method is the same as that in Example 2.

[0097] The HE staining solution prepared throug...

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Abstract

The invention provides a hematoxylin staining solution which comprises the following components of hematoxylin, aluminium salt, an oxidizing agent, benzalkonium chloride, alcohol, weak acid and water. The invention further provides a preparation method of the hematoxylin staining solution, a HE (hematoxylin eosin) staining solution containing the hematoxylin staining solution, and a staining method. When the hematoxylin staining solution is used to stain a section, the background of the section is clear, the section is well arranged, the staining effect is excellent, the section is easy to read, the color of the section does not fade easily, and the section can be stored conveniently for a long period of time.

Description

technical field [0001] The invention relates to an in vitro diagnostic reagent, in particular to a hematoxylin staining solution and a preparation method thereof, as well as an HE staining solution containing the hematoxylin staining solution and a staining method thereof. Background technique [0002] Hematoxylin and eosin staining, referred to as HE staining, is the most widely used technique in histology, embryology, and pathology. HE staining is to immerse tissue sections or cell smears in hematoxylin staining solution and eosin staining solution respectively, and after appropriate time and treatment, the tissues, cells and other components are stained with different shades of color, resulting in different refractive indices. It is convenient for observation and research under an optical microscope. [0003] Hematoxylin is a kind of basic dye, which is extracted from logan and is oxidized to generate hematin. It is used together with mordant to make the chromatin in the...

Claims

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Application Information

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IPC IPC(8): C09B67/00C09B67/22G01N1/30
Inventor 邹霈吴军吴昊谢敏浩王洪勇刘娅灵
Owner JIANGSU INST OF NUCLEAR MEDICINE
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