33 results about "Embryology" patented technology

The invention relates to an oryzias melastigma fertilized egg hatching method, and belongs to the field of aquaculture. According to the oryzias melastigma fertilized egg hatching method, the fertilized eggs are hatched in seawater with different salinity gradients, the physical and chemical conditions for hatching are the temperature being 26-28 DEG C, the humidity being 40-60%, the light intensity being 80-125[mu]mol.m-2.s-1, and the light-dark ratio being 14:10. The fertilized eggs with high cleanliness can be obtained, experimental operations such as embryology experiment observation and microinjection can be carried out conveniently, and the hatching rate of the fertilized eggs can be as high as 94.7% by using the oryzias melastigma fertilized egg hatching method. Moreover, hatched oryzias melastigma can develop, grow and reproduce normally after the long-term observation, and no abnormal phenomena are found.

The invention discloses a cell culture box for histogenetic experiments, comprising a box body, a box door and a culture placing rack. A box door is hingedly installed on one side of the box body through hinges, and the top of the box body and the An ultraviolet germicidal lamp and a heater are respectively fixedly installed at the bottom, a plurality of culture placement racks are installed inside the box body, a base is installed at the bottom of the box body, and the culture placement racks pass through the notch on one side of the box body Installed with the box, the culture placement rack includes a U-shaped placement slot and an end baffle installed on one end of the U-shaped placement slot, the U-shaped placement slot is fixedly installed with a cell culture dish, and one of the U-shaped placement slot is installed A sliding block is installed on the side, and the culture placing rack is slidably installed with the box body through the sliding block and the sliding rail on the box body. The invention has a compact structure, can realize the efficient culture of cells for histoembryology experiments, is less affected by external interference, has less pollution, is simple in operation, and is very flexible in use, and can improve the culture quality of the cells in the incubator.

A method for producing a monkey-derived embryonic stem cell comprising the steps of carrying out fertilization by insemination by in vitro fertilization or intracytoplasmic sperm injection using a monkey ovum and monkey sperms, thereby giving a fertilized ovum, allowing the fertilized ovum to differentiate into a blastocyst by in vitro culture, and establishing an ES cell line using the blastocyst; the monkey ES cell obtained by the method, a method for screening a reagent for specific differentiation into cell or tissue by using the ES cell; and a differentiated cell or differentiated tissue each differentiated from the ES cell. According to the present invention, applications of the embryonic stem cells to embryological studies clinical applications, experimental models, and the like on primates, studies of diseases, are expected.

The invention discloses a method for preparing a composite door panel. The operation steps are as follows: (1) cutting: decontamination treatment is performed on the raw materials, ingredients are prepared according to the requirements of the drawing, and the wooden frame, filler board and surface board are cut; (2) high temperature treatment : Carry out high-temperature treatment on the wooden frame, filler board, and surface board; (3) Thickness sanding; (4) Gluing and forming embryos; (5) Glue pressing: Send the slabs to the hot press, start the hot press, The temperature is controlled at 150°-170°, the compression time is 200s-300s, and the pressure is 4MPA-6MPA. After the hot pressing is completed, let it stand for 30-45min and remove the slab; (6) veneer the cooled slab Edge sealing, processing forming edge, processing assembly hole for assembly; (7) Spraying topcoat. The invention improves the preparation method, effectively prevents degumming and even warping of the composite board by controlling the moisture content of the composite board, and improves the appearance quality of the composite board.

The invention provides a device for extracting and delivering germ cells in the in-vivo experimental rat genital tract and belongs to the technical field of animal embryology. The device for extracting and delivering the germ cells in the in-vivo experimental rat genital tract consists of a moving suction ball (1), a suction and delivery tube (2), an operating bag (3), a computer data line (39) and a control computer (40) and is characterized in that the moving suction ball (1) is olive-shaped, the cross section of the moving suction ball (1) is oval, the longest diameter of the moving suction ball (1) ranges from 0.5 cm to 1 cm, and the shortest diameter of the moving suction ball (1) ranges from 0.05 cm to 0.3 cm. A ball top suction opening (4), a camera (28) and a flash lamp (29) are arranged at the front end. Two to five circles of forwards-moving tube feet (6) are arranged at the front half portion, four to eight ball waist suction openings (5) are arranged in the middle, two to five circles of receding tube feet (7) are arranged at the rear half portion, a ball tube connector (8) is arranged at the rear end and enables the moving suction ball (1) and the suction and delivery tube (2) to be connected together. The device for extracting and delivering the germ cells in the in-vivo experimental rat genital tract is simple in manufacture, good in operability, low in cost and obvious in effect.

The present invention is directed to the seed-to-seed perpetuation of hybrid vigor and other traits through apomixis (asexual seed formation) in flowering plants (angiosperms). More particularly, to predictable methods for producing, from sexual or facultatively-apomictic plants, progeny plants that express an increased percentage of apomictic seed set or one or more elements of apomixis. This invention uses: plant cytoembryology procedures to identify and select a plant or group of plants that possess appropriate genetic variability for initiation times and durations of megasporogenesis (female meiosis), embryo sac formation, egg and central cell formation and maturation, fertilization, embryony and endosperm formation; plant breeding procedures to produce numerous and divergent genetically-recombined early to late generation progeny such that embryo sac formation preempts megasporogenesis and embryony preempts fertilization; and plant cyto-embryology or progeny test procedures to select segregant plants that express an increased frequency of one or more elements of apomixis.

The invention discloses an embryology virtual simulation teaching system fused with a VR game, the teaching system comprises a knowledge explanation module, an animation game practice module and a knowledge assessment module, the knowledge explanation module is used for clicking introduction and playing theoretical knowledge explaining embryology; the animation game practice module is used for constructing and displaying the whole process of sperm-egg combination in embryology in a VR animation game mode, and further understanding and learning embryology knowledge; and the knowledge assessment module is used for assessing theoretical knowledge of embryology in a time-limited manner. By fusing the VR game, the process of combining the sperms and the ova is constructed in a game mode, the whole process of combining the sperms and the ova is vividly displayed, teaching and science popularization application are better carried out, and the integration of academic teaching and the virtual technology is realized.

The invention discloses an application method of the sixth finger in histology and embryology experiment teaching. The sixth finger can be obtained through operation; after the fixation of a fixation solution of a certain concentration, the sixth finger is subjected to dehydration, hyalinization, waxdipping, embedding, slicing, dyeing and slice packaging in a certain manner; and finally, the sixth finger is used for observation of epithelial tissue, connective tissue, cartilage and bone and muscular tissue in histology and embryology experiment teaching, so that the problem that it is not easy to obtain a normal human tissue sample in the histology and embryology experiment teaching is solved, and effective utilization of experiment resources is expanded. Besides, the sixth finger is easily available, can be obtained from an operating room of the department of orthopaedics, can be obtained timely, and can be applied to the scientific research of human tissue engineering; the cell culture technique is adopted to separate epithelial cells, adipose cells and osteoblasts of the sixth finger, and thus the obtained cells are closer to real cell experiment; and the sixth finger is used for scientific experiment researches.