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A kind of method and application of cultivating polyploid poplar

A technology of polyploidy and poplar, applied in the fields of application, botany equipment and methods, cultivation, etc., can solve the problems of high technical level requirements for aseptic operation, complicated operation steps, and heavy experimental workload, etc., and achieve the goal of improving breeding Efficiency, shorter breeding cycle, and less work

Active Publication Date: 2021-08-31
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This tetraploid induction method is highly dependent on sterile tissue culture operations. It is necessary to establish an efficient leaf adventitious bud regeneration system. The experimental workload is large, the cycle is long, the operation steps are complicated, and the technical level of aseptic operation is high.

Method used

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  • A kind of method and application of cultivating polyploid poplar
  • A kind of method and application of cultivating polyploid poplar
  • A kind of method and application of cultivating polyploid poplar

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The poplar test material selection research group used in this embodiment collected the hard branch cuttings of the diploid poplar species 'Huilin 88' from Tongliao Academy of Forestry, Inner Mongolia, with a thickness of 2.2 cm and a length of 12 cm.

[0041] 1. Cuttings

[0042] In the greenhouse of Beijing Forestry University, the cuttings of 'Huilin 88' were placed in a flowerpot of 25cm (inner diameter) × 25cm (height), and the cutting substrate sterilized by high temperature and high pressure was filled in the flowerpot, and the substrate was peat soil: Perlite: river sand = 2:1:1, water the cuttings thoroughly, cover with plastic film and cultivate at 15-25°C and 50-80% relative humidity.

[0043] 2. Double processing

[0044] About 3 days after cutting, callus formation can be observed in the cambium area of ​​the cutting surface ( figure 1 ), the callus developed further after cutting 8 days. At this point, completely cover the cut surface of the cuttings with...

Embodiment 2

[0052] The test material of this embodiment is the tetraploid hard branch cuttings of Populus papillodis that were induced by the research group in the early stage, with a thickness of 2.6 cm and a length of 12 cm.

[0053] 1. Cuttings

[0054] In the greenhouse of Beijing Forestry University, the cuttings of the tetraploid Populus pachyrhiza were cut in a flowerpot of 25cm (inside diameter) × 25cm (height), and the cutting substrate sterilized by high temperature and high pressure was filled in the flowerpot, and the substrate was peat soil : perlite: river sand = 2:1:1, water the cuttings thoroughly, and cover with plastic film to cultivate at 15-25°C and 50-80% relative humidity.

[0055] 2. Double processing

[0056] About 5 days after cutting, callus formation can be observed in the cambium area of ​​the cutting surface, and the callus further develops after 10 days of cutting. At this point, completely cover the cut surface of the cuttings with absorbent cotton, add dr...

Embodiment 3

[0064] The poplar test material selection research group used in this embodiment collected the hard branch cuttings of the diploid poplar species 'Huilin 88' from Tongliao Academy of Forestry, Inner Mongolia, with a thickness of 2.2 cm and a length of 12 cm.

[0065] 1. Hydroponics

[0066] In the greenhouse of Beijing Forestry University, the hard branch cuttings of 'Huilin 88' were hydroponically cultured in a 10cm (upper inner diameter) × 20cm (height) plastic water cup filled with tap water and placed at 15-25°C and 50-80°C. % relative humidity and change the water every 2 days.

[0067] 2. Double processing

[0068] After hydroponics for about 6 days, callus formation can be observed in the cambium area of ​​the cutting surface, and the callus develops further after hydroculture for 10 days. At this point, completely cover the cut surface of the cuttings with absorbent cotton, add dropwise 0.05% colchicine solution to avoid light for 24 hours, remove the absorbent cotto...

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Abstract

The invention relates to a method for cultivating polyploid poplar and its application. The method comprises inserting poplar branches into a culture medium and cultivating them at 15-25°C and 50-80% relative humidity for 3-10 days. Incisions are made on the poplar branches to form callus; polyploid mutagen is used to treat the callus until the mutagenesis is sufficient, and the culture is continued after the treatment is removed. The present invention finds that the cuts on poplar cutting branches can be cultivated under suitable temperature and humidity conditions to form callus, and further treatment with polyploid mutagen can realize the cultivation of polyploid poplar, and the induction rate reaches about 42.9%. . The present invention solves the problems of poplar somatic cell chromosome doubling process or relying on embryological observation or tissue culture in the past, and has the advantages of easy operation, low cost, high induction efficiency, high preservation rate, etc. Seedling formation is an ideal way for poplar somatic chromosome doubling.

Description

technical field [0001] The invention relates to the technical field of plant breeding, in particular to a method for cultivating polyploid poplars and an application thereof. Background technique [0002] The importance of polyploidy breeding for poplar genetic improvement has been widely recognized (Kang XY. Polyploid induction techniques and breeding strategies in poplar. In: Mason AS(ed) Polyploidy and hybridization for crop improvement. CRC Press, Boca Raton, 2016, pp 76–96), polyploid poplar species have exerted great value in existing forestry production. Somatic chromosome doubling is one of the important ways to induce polyploidy in poplar. This pathway is mainly to use chromosome doubling mutagen to treat somatic cells with meristematic ability to inhibit the separation of sister chromatids in metaphase of mitosis, thereby forming doubled cells and obtaining polyploidy. Somatic cell chromosome doubling is a very suitable autopolyploid induction method that can hig...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/08A01G2/10A01G24/10A01G24/12A01G24/15A01G24/28A01G31/00
CPCA01G31/00A01H1/08A01G2/10A01G24/10A01G24/12A01G24/15A01G24/28
Inventor 王君钟宇航李代丽
Owner BEIJING FORESTRY UNIVERSITY
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