139 results about "Mutagen" patented technology

A strain of Trichoderma harzianum is obtained that is useful as a nematode inhibitor, fungicide and plant growth promoter. The strain has ATCC accession number PTA-3701. The strain is isolated by treating Trichoderma harzianum isolated from experimental fields of Central Institute of Medicinal and Aromatic Plants (CIMAP) Field Station with a mutagen such as ethyl methyl sulphonate, and isolating a whitish and fast growing strain of Trichoderma harzianum.

The present invention provides non-petroleum high-octane fuel derived from biomass sources, and a method of producing same. The method of production involves reducing the biomass feedstocks to sugars, fermenting the sugars using microorganisms or mutagens thereof to produce ethanol or acetic acid, converting the acetic acid or ethanol to acetone, and converting the acetone to mesitylene and isopentane, the major components of the renewable engine fuel. Trimerization of acetone can be carried out in the presence of a catalyst containing at least one metal selected from the group consisting of niobium, iron and manganese. The ethanol can be converted to mesitylene in a dehydration reaction in the presence of a catalyst of zinc oxide / calcium oxide, and unreacted ethanol and water separated from mesitylene by distillation. These ethanol-based, biomass-derived fuels are fully renewable, may be formulated to have a wide range of octane values and energy, and may effectively be used to replace 100 LL aviation fuel (known as AvGas), as well as high-octane, rocket, diesel, turbine engine fuels, as well as two-cycle, spark-ignited engine fuels.

Methods of using a transposon as an insertional mutagen are provided. Also provided is a transgenic animal that includes polynucleotides encoding a transposon and transposase that can be used to identify genomic sequences. The methods and transgenic animals may be used to detect cancer-related genes by identifying common insertion sites in tumor cells.

A mutagenic, triplex-forming oligonucleotide and methods for use thereof wherein the oligonucleotide is chemically modified to incorporate a mutagen and forms a triple-stranded nucleic acid molecule with a specific DNA segment of a target DNA molecule. Upon formation of the triplex, the mutagen is brought into proximity with the target molecule and causes a mutation at a specific site therein. The mutation activates, inactivates or alters the activity and function of the target molecule.

The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid. Typically the solid or semi-solid support is selected from the group consisting of a polymeric gel, a membrane, an array, a glass bead, a glass slide, and a polymeric microparticle. Preferably, the polymeric gel is agarose or polyacrylamide. The methods employing the non-genotoxic compounds represent an improvement over commonly used methods employing ethidium bromide wherein the present methods retain the advantages of ethidium bromide, ease of use and low cost, but without the disadvantageous, known mutagen requiring special handling and waste procedures.

The invention relates to compositions comprising acyl derivatives of 2'-deoxyribonucleosides. The invention also relates to methods of treating or preventing radiation, mutagen and sunlight-induced cellular damage, methods for improving wound healing and tissue repair, and methods for ameliorating the effects of aging comprising administering the compositions of the present invention to an animal.

A code optimizing HPV16 E6-E7 fused gene, fused gene with carcinogenicity elimination by fixed-point mutagen and it encoded fused protein are disclosed. The modified fused genes are increased in mammal cell expression. It has excellent cell conversion activity and biological safety. It can be used to offer excellent provisional antigen gene for constructing related DNA vaccine.

The present invention discloses a method for screening yellow wine yeast of high-yield urea. The method can screen out the yeast colony with low urea yield from the yellow wine yeast mutagenized by chemical mutagen with a canavanine plate and can also screen out the yeast colony with better low-yield property from the yellow wine yeast colony of common low-yield urea. The method for screening according to the invention can quick separate and obtain the target mycopremna. The blindness of breeding process is reduced and the predictability is increased. The breeding efficiency is greatly increased. The yellow wine yeast obtained through screening has the characteristic of low content of byproduct urea and can effectively reduce the content of urethane in the production of yellow wine.

The invention discloses a screening method of grape wine yeast with low-yield ethyl carbamate and application of grape wine yeast in the production of grape wine. The screening method comprises the following steps of: screening out a bacterial strain with the low-yield ethyl carbamate from active dried yeast and mutant strains by adopting a structural analogue of arginine, i.e., canavanine resistance flat plate, wherein the activity dried yeast can be separated from nature or can be purchased in market, and the mutant strains are obtained through the mutagenesis of chemical mutagen. The method is designed by a yeast amino acid metabolism theory and is simple and convenient; and a target bacterial strain can be obtained through rapid separation. According to the screening method, the obtained resistance bacterial strain B1 can be numbered as F15-1-15 bacterial strain (CGMCC (China General Microbiological Culture Collection Center) NO. 4727) and is applied to the brewing of the grape wine in a small-sized fermented container; and on the basis of excellent brewing performance of the grape wine, the bacterial strain has the advantages of being capable of effectively and obviously reducing the content of the ethyl carbamate in the production of the grape wine and having the potential of industrial production and application.

The invention relates to Trametes Versicolor YS-L613 and a breeding method and application thereof. The Trametes Versicolor YS-L613 is prepared by the method through mutation breeding. The breeding method is to take Trametes Versicolor as a starting strain, adopt composite mutation breeding technology which combines UV, 60 CO, ultraviolet rays, nitrosoguanidine, ion implantation and microwave treatment, and breed the YS-L613 strain for high-yield production of laccase. The invention performs breeding operation through physiochemical treatment methods such as ion implantation, the ultraviolet rays and mutagen, finally obtains the YS-L613 strain for high-yield production of the laccase, and adopts experimental automatic control technology, strain fermentation control technology and strain extraction technology to develop laccase products. As proved by experiments of the laccase in pulp bleaching, fiber modification and pulp deinking, utilization of the laccase in the paper-making industry can improve the quality of paper, reduce environmental pollution and save the cost.

The invention discloses a seed breeding method of moso bamboo by physical and chemical composite mutagenesis. The seed breeding method comprises the following five steps of: (1) collection of the moso bamboo seeds, wherein the purity of the seeds is more than or equal to 90%, the thousand seed weight is more than or equal to 28g, the moisture content is 10%-14%, and after being sealed, the seeds are stored for later use at the temperature of 4 DEG C; (2) irradiation ray selection of caesium Gamma rays with the atomic weight being 137 as an irradiation source, and selection of two types of mutagen treating liquid; (3) preparation of the two types of mutagen treating liquid; (4) physical and chemical composite mutagenesis treatment, wherein the radiation dose rate is 1Gy / min, the radiation dose is 30Gy, the seeds are soaked for tens of hours with warm water, after water-absorbing paper absorbs the surface water of the seeds completely, the seeds are soaked and mutagenized for eight hours by using a sodium azide solution (NaN3) or ethylmethane sulfonate (EMS) solution with the concentration of 0.04mmol / L, and after being taken, the seeds are cleaned repeatedly for 2 hours by using clean water to obtain the moso bamboo seeds treated by composite mutagenesis; and (5) germination test of the seeds, verification of mutagenic effect and determination of mutagenesis dose. The seed breeding method disclosed by the method has the advantages that the mutants in mutagenesis are more in quantity, the character mutation is diversified, and the selection range of excellent characters needed in seed breeding is large.

The invention discloses a Cordyceps sinensis mutant strain for production of Cordyceps sinensis mycelia and relates to the field of application of a food microorganism technology. The mutant strain is collected in CCTCC (China Center for Type Culture Collection) in Wuhan, China on Jan. 23rd, 2015 with the number of CCTCC M 2015064 and the recommended Latin name of Cordyceps sinensis CCTCC M 2015064. In order to avoid use of toxic and harmful chemical mutagens and a genetic engineering method which is not recognized by the public in the food field, an ultraviolet physical mutagenesis technology is considered to be simple and easy, so that the ultraviolet mutagenesis method is adopted for mutagenesis, excellent characters of the novel Cordyceps sinensis strain are guaranteed through a series of screening methods in aspects of stability, hereditary and the like, and a strain basis for obtaining better-quality Cordyceps sinensis mycelium raw material, which can be used for producing healthy food, for industrial efficient fermentation of low-cost and novel rice bran and wheat bran complete liquid culture media to which cysteine hydrochloride and sodium copper chlorophyllin are added.

The invention relates to compositions comprising acyl derivatives of 2'-deoxyribonucleosides. The invention also relates to methods of treating or preventing radiation, mutagen and sunlight-induced cellular damage, methods for improving wound healing and tissue repair, and methods for ameliorating the effects of aging comprising administering the compositions of the present invention to an animal.

The invention relates to compositions comprising acyl derivatives of 2'-deoxyribonucleosides. The invention also relates to methods of treating or preventing radiation, mutagen and sunlight-induced biological damage, and methods for improving wound healing and tissue repair, comprising administering the compositions of the present invention to an animal.

The invention involves a kind of Ganoderma Lucidum that can produce saccha-peptide complexes in high yield, the method of mutagenesis and selection, the purpose of it. The name of the fungi is Ganoderma Lucidum according to Systematic nomenclature, held by 2004-11-22 in <the general microbiology center of china culture collection managerial committee >, and the holding number is CGMCC No.1251. The first step of mutagenesis and selection is to culture the Ganoderma Lucidum CAU55 which is bought from <the general microbiology center of china culture collection managerial committee > as the initial strain and the holding number of which is CGMCC 5.533 in solid medium and fluid medium in turn and get the mycelia solution of the initial strain CAU55. The second step is to treat the mycelia solution with enzymolysis and filtration and get the protoplast deposit of the initial strain CAU55. The last step is to treat the protoplast with ultraviolet mutagenesis and regenerative cultivation, in the end getting the Ganoderma Lucidum CAU5501 that can produce saccha-peptide complexes in high yield. This kind of Ganoderma Lucidum is useful for preparing the feed additive of saccha-peptide complexes from liquid fermentation and some microbiological pharmaceutics like immunopotentiator and beneficial biotin.

It is intended to utilize β-glucan produced by a bacterium belonging to Aureobasidium sp. From a bacterium belonging to Aureobasidium sp., a mutant with little pigment accumulation is constructed by a mutagenesis means of, for example, irradiating with ultraviolet light or treating with a mutagen. A culture obtained by culturing this mutant in a liquid culture medium is usable as a composition with a large β-glucan content without showing any intense dark green color caused by the accumulation of melanin-like pigments. This composition may be taken as such as a functional food having the physiologically active functions of the β-glucan-containing composition. Alternatively, it may be added to foods, drinks. food additives, cosmetics and so on.

The present invention provides a genetically modified plant that biosynthesizes an increased amount of capsinoids, a method of producing the genetically modified plant, and a production method of capsinoids from the genetically modified plant. More particularly, the present invention provides a genetically modified plant capable of producing capsinoids, which shows a decreased expression or activity of an enzyme that catalyzes an amino group conversion reaction from vanillin to vanillylamine as compared to wild strains, and the like. As a method of suppressing expression or activity of an enzyme, an introduction of DNA encoding an antisense RNA, iRNA, ribozyme or dominant-negative mutant for the target gene, a destruction of the gene by a knockout method, mutagen treatment or transposon insertion, an introduction of a gene encoding an antibody against the enzyme and the like can be mentioned.

The invention mainly provides a medicago sativa plant resistant to herbicide and a method, particularly relates to a method for screening medicago sativa plants resistant to the herbicide by utilizing chemical mutagen EMS (ethyl methane sulfonate) for mutagenesis of medicago sativa, and discloses a culture medium screening method for glyphosate-resisting medicago sativa plants. The method is characterized by including the following steps: (1) germinating seeds by an MS culture medium; (2) performing dosage screening; (3) subjecting medicago sativa seeds to mutagenesis by median lethal dose of the EMS; (4) screening the glyphosate-resisting medicago sativa plants; (5) rescreening the resistant plants. The method has the advantages of being simple and easy to implement, taking effect fast and capable of allowing single plants of herbicide-resistant medicago sativa to be obtained within a short time. A mutagenesis way is adopted. The chemical mutagen EMS is utilized to mutate the medicago sativa seeds to generate point mutation, the single plants of the herbicide-resistant medicago sativa are screened by two ways of the culture medium and soil screening, new germplasm resources are provided for conventional breeding, and certain references are provided for herbicide-resistant breeding of other plants.

The invention relates to a method for constructing a heart cell system of blotchy rockcod, which comprises: taking a heart tissue of the blotchy rockcod as a material, adopting a trypsin digestion method to start primary culture, and culturing the heart tissue in a DMEM / F12 liquid medium which contains fetal calf serum, basic fibroblast growth factors, I-type insulin-like growth factors, chondroitin sulfate and culture supernatant of fin cells of the blotchy rockcod in the log phase and has a pH value of between 7.0 and 7.4; and adopting the trypsin digestion method for subculturing. The heart cell system of the blotchy rockcod constructed by the method is subcultured for 70 generations currently. The technology is scientific and reasonable, is hopeful to be applied to separation and breeding of fish viroids, preparation of virus vaccines and research in the aspects of interaction of viruses and host cells, and so on, can also be taken as a model for researching environmental pollutants in environmental toxicology, and performs pollution monitoring and safety evaluation on various environmental pollutants in the sea such as genic toxins, mutagen, carcinogens, environmental hormones and endocrine disruptors.

The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid. Typically the solid or semi-solid support is selected from the group consisting of a polymeric gel, a membrane, an array, a glass bead, a glass slide, and a polymeric microparticle. Preferably, the polymeric gel is agarose or polyacrylamide. The methods employing the non-genotoxic compounds represent an improvement over commonly used methods employing ethidium bromide wherein the present methods retain the advantages of ethidium bromide, ease of use and low cost, but without the disadvantageous, known mutagen requiring special handling and waste procedures.

The invention relates to an allopolyploid induction growth method of hybridized Chinese pennisetum. The method comprises the following steps: immersion of allotriploid hybridized Chinese pennisetum F1 seeds in a mutagen containing colchicine and dimethylsulfoxide, initial culture, polyploid identification, proliferation culture, expanded production and transplanting. The method allows a large number of mutagenic seedlings to be obtained in a short time, greatly improves the culture efficiency and saves the production cost.

The invention discloses a breeding method of strong-acid resistant lactic acid bacteria strain. The breeding method includes inducing common probiotic lactic acid bacteria by chemical mutagen such as nitrosoguanidine, exploring massive mutation colony of lactic acid as a new lactic acid genetic germplasm resource library, screening mutant strains, which are capable of growing on lactobacillus culture medium MRS and have regular shape and distinctive characteristics, from the resource library, and screening strains that could multiply fast after being treated by simulated gastric fluid from the mutant strains, which are the novel high-quality and strong-acid resistant lactic acid bacteria strain.

The invention provides a liquid submerged culture method of Ergosterol Hericium erinaceus. On the basis of preliminary screening, the strains of Hericium erinaceus growing fast on the solid medium are screened; combined with a physical mutagen, ultraviolet mutagenesis is carried out to obtain a A high-yield ergosterol-producing Hericium erinaceus strain suitable for submerged liquid culture. And through the optimization of the culture medium and culture conditions, the liquid submerged culture method of the Hericium erinaceus is determined, which can shorten the period of industrial production of mycelium and increase the biological yield.

The invention provides a wheat mutation breeding method and aims to improve the chemical mutagenesis efficiency, generate a relative high point mutation frequency and have relative little chromosomal aberration. The wheat mutation breeding method disclosed by the invention adopts a 0.5% EMS (Ethylmethane Sulfonate)-phosphoric acid buffering solution to treat wheat pollen to induce andro gametes to have variation so as to obtain variant seeds; the 0.5% EMS-phosphoric acid buffering solution takes a phosphoric acid buffering solution as a solvent and a chemical mutagen ethylmethane sulfonate EMS is diluted to the volume ratio concentration of 0.5%; the mol concentration of the phosphoric acid buffering solution is 1 / 15mol / L; the dosage of the 0.5% EMS-phosphoric acid buffering solution is 25-50 microliters on each floret; and the wheat pollen is pollen which is about to be ripe or pollen grains which are primarily ripe.

The invention is a space-simulated seed mutagenic device, comprising an X-ray generator (1), a microwave generator (2), an ultraviolet generator (3), an ultrasonic generator (4), a gamma-ray generator (5), a valve (6), an external storehouse (7), an internal storehouse (8), an air blower (9), a fixed frame (10) and a sealing board (11); the external storehouse (7) and the internal storehouse (8) are spheroidal and are provided with an air-tight door which is open and the sealing board (11) is arranged between the external storehouse (7) and the internal storehouse (8); the sealing board (11), the external storehouse (7) and the internal storehouse (8) form a seal cavity; the internal storehouse (8) is provided with a hole; the external storehouse (7) is provided with the valve (6), and the X-ray generator (1), the microwave generator (2), the ultraviolet generator (3), the ultrasonic generator (4) and the gamma-ray generator (5) are arranged in the external storehouse (7); the fixed frame (10) is provided with a cavity, and an air inlet which is connected with the air blower (9) is arranged at the bottom of the fixed frame (10); the external storehouse (8) is arranged in the cavity of the fixed frame (10); therefore, the mutagen can be concentrated in one channel for radiating the seeds in an all-round way and the injury on the seeds is little.

An assembly and method for processing human waste includes providing a supporting base having an opening within which a waste reservoir is affixed. The assembly may be installed on or below a rim of a toilet bowl. Solidification and pathogen killing materials are provided. The materials are deposited in the reservoir either prior to or upon accumulation of waste in the reservoir. The reservoir with the solidified waste is then sealed and transported to a collection facility.

The invention mainly relates to a screening method of anti-glufosinate-ammonium alfalfa plants, in particular to an anti-glufosinate-ammonium germplasm screening method. A screening method for a culture medium for anti-glufosinate-ammonium alfalfa plants is mainly characterized by comprising the following steps: (1) germinating seeds by the MS (Murashige and Skoog) culture medium; (2) screening the dose; (3) inducing alfalfa seeds through ethylmethane sulfonate median lethal dose; (4) screening the anti-glufosinate-ammonium alfalfa plants; and (5) screening resistant plants again. Compared with the prior art, the method has the advantages of being simple and easy to implement, taking effect quickly, and being capable of obtaining the herbicide resistant alfalfa individual plant in a short term. The method adopts a mutation measure, utilizes a chemical mutagen EMS (ethylmethane sulfonate) to induce the alfalfa seeds to cause point mutation, can screen the herbicide resistant alfalfa individual plant by the culture medium and the soil screening method, can provide a new germplasm resource for conventional breeding and can provide a reference for breeding of herbicide resistant agents of other plants.