Ergosterol hericium erinaceus liquid deep culture method

A liquid deep culture, ergosterol technology, applied in the biological field, can solve the problem that the quality of raw materials cannot be guaranteed, the production performance of fruiting bodies is unstable, etc., and achieve the effects of shortening the growth time, shortening the cycle, and increasing the ergosterol content.

Inactive Publication Date: 2016-10-12
张岩
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Problems solved by technology

[0003] At present, most of the researches focus on the fruiting body of Hericium erinaceus, but the production performance of the fruiting body is unstable, and the requirement for stable quality of raw materials cannot be guaranteed. However, the liquid submerged fermentation has a short production cycle, consistent bacterial age, fast mycelium growth rate, simple inoculation, and no Restricted by the season, it can be produced every year, which ensures the controllability of Hericium erinaceus in the production process and the quality stability of the target product

Method used

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  • Ergosterol hericium erinaceus liquid deep culture method

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Embodiment 1

[0016] 1. Strains

[0017] Hericium erinaceus strains were preserved in the laboratory of the School of Biology and Food Engineering, Tianjin Tianshi University.

[0018] 2. Medium

[0019] 2.1 Inclined plane and plate culture medium

[0020] Potato 30% (boiled juice), peptone 0.5%, glucose 2%, agar 1.3%, pH natural.

[0021] 2.2 Preliminary screening of liquid culture medium

[0022] Dissolve with 2% sawdust extract juice: glucose 2%, peptone 0.5%, potassium dihydrogen phosphate 0.15%, magnesium sulfate 0.075%, pH natural.

[0023] 2.3 Liquid fermentation medium

[0024] Basic medium for single factor experiment: 2% glucose, 0.5% peptone, 0.15% potassium dihydrogen phosphate, 0.075% magnesium sulfate, natural pH.

[0025] Select carbon sources: glucose, sucrose, soluble starch, malt extract powder, glucose + soluble starch; nitrogen sources: peptone, yeast extract, tryptone, beef extract; inorganic salts: potassium dihydrogen phosphate, magnesium sulfate, manganese sulfa...

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Abstract

The invention provides a liquid submerged culture method of Ergosterol Hericium erinaceus. On the basis of preliminary screening, the strains of Hericium erinaceus growing fast on the solid medium are screened; combined with a physical mutagen, ultraviolet mutagenesis is carried out to obtain a A high-yield ergosterol-producing Hericium erinaceus strain suitable for submerged liquid culture. And through the optimization of the culture medium and culture conditions, the liquid submerged culture method of the Hericium erinaceus is determined, which can shorten the period of industrial production of mycelium and increase the biological yield.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a liquid submerged culture method for ergosterol Hericium erinaceus. Background technique [0002] Hericium erinaceus, also known as monkey cabbage, Hericium erinaceus, and opposite face mushroom, belongs to the large edible and medicinal fungi of the genus Hericium erinaceae of the Basidiomycetes subphylum. It has a very good protective effect. Among them, sterols have the functions of lowering blood fat, preventing cardiovascular diseases, and delaying aging. They are also precursors for the synthesis of various hormones and vitamin D, and play an important role in material transportation. In recent years, the demand of Chinese consumers for ergosterol has been increasing year by year, and the breeding of strains with high ergosterol production is particularly important. [0003] At present, most of the researches focus on the fruiting body of Hericium erinaceus, but the productio...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N15/01C12P33/00C12R1/645
CPCC12N1/14C12N15/01C12P33/00
Inventor 蔡佳佳张岩邢春玉郭美李雪莹白芯语
Owner 张岩
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