The invention provides a method for rapidly reproducing high quality germchit with
dendrobium officinale, which is characterized in that: (1) folded sprouts of the
dendrobium officinale are taken and sterilized, and stem apexes are inoculated to a
solid culture medium to establish a non-bacterial
system; (2) the stem sections or small basal tissue blocks of the young seedlings of the non-bacterial
system are
cut for inducing protocorm based on suitable
hormone combination and a culture medium; (3) the protocorm is cultured alternatively on liquid and
solid subculture mediums for rapid multiplication to form protocorm masses; (4) the protocorm masses are
cut apart and transferred on a
solid planting medium for disuniting plants into small seedlings; (5) the small seedlings are transferred on a solid strong
seedling culture medium for strengthening the seedlings and taking roots to culture complete plants; (6) the non-bacterial strong seedlings are fixedly planted in suitable
seedling adapting substrate to obtain the high quality germchit. The method can be used in large scale industrialization and the explants have the advantages of high soil removal efficiency, high
multiplication rate, low variability, strong germchit, good quality,
high survival rate after being transplanted, strong growth potential, etc.