Method and apparatus for detecting drug residue of food

Abstract

The invention discloses an immunity chromatography device for checking left drug or toxin, comprising a solid medium containing the drug or toxin antibody with a colloid metal mark, and a fiber membrane connected with one end of the solid medium, wherein the fiber membrane is fixed with the drug and toxin. The invention also discloses a relative preparation method of the drug or toxin antibody with the colloid metal mark, and a method for using the device to check the left drug or toxin, with high sensitivity.

Description

technical field [0001] The invention mainly relates to a device and a method for detecting drug or toxin residues in animal food, especially a test paper for detecting ractopamine and an application method thereof. The present invention also relates to the above detection equipment and the manufacturing method of the key colloidal metal. Background technique [0002] In the breeding of food animals, illegal use or excessive use of ractopamine, clenbuterol hydrochloride, chloramphenicol and other drugs is common, and some even caused serious damage to people's health; Residues of toxins such as botulinum toxin are also necessary, so efficient and sensitive detection methods are needed to effectively prevent such animal food from entering the consumer market. For example, ractopamine (Ractopamine, abbreviated as Rac herein) is a phenylethanolamine β2-adrenoceptor agonist, which can selectively excite the β2 receptors of smooth muscle, and is mainly used clinically for the tre...

AI Technical Summary

Problems solved by technology

[0005] However, the above prior art has the following disadvantages: 1. The colloidal metal-labeled drug or toxin antibody is mixed with unlabeled drug or toxin antibody, which will competitively inhibit the drug or toxin antibody in the detection sample after it is made into a detection device. Toxins, or colloidal metals that are not bound to antibodies, affect the binding speed of drugs or toxins in the sample and colloidal metal-labeled antibodies through steric hindrance, and interfere with the detection results; 2. When mixing antibodies and colloids to make them bind, Due to the high concentration of local antibodies, the colloidal gold particles that are exposed to antibodies first will bind to multiple antibodies, and then the colloidal gold particles that are exposed to antibodies may bind to one antibody molecule or no antibody. The heterogeneity of the drug or toxin antibody is high, which affects the test results; 3. The protein or macromolecular reagents such as BSA or PEG used for blocking will make the sample in the test due to the existence of steric hindrance. Drugs or toxins cannot be well contacted with the labeled antibody, which affects the sensitivity of the detection

This technology needs to add BSA or PEG after mixing the antibody with colloidal gold, and purify by simple centrifugation and reconstitution, so it has the above disadvantages, which is not conducive to improving the sensitivity and accuracy of detection

The ractopamine residue rapid detection test strip prepared in this way has the following deficiencies in the application process: 1. When ractopamine in the sample is negative, the red band at the detection line is too light in color, which is not conducive to observation; When ractopamine is positive, there is still a faint red band at the test line, which cannot completely disappear

Therefore, if the drug concentration in the sample exceeds the standard, but the red band at the detection line is too light or disappears, the occurrence of this situation will seriously interfere with the judgment of the testing personnel, and it is likely that the sample is considered negative, resulting in missed detection

These situations make the inspectors feel at a loss in practical application, hinder the popularization and application of rapid detection test equipment (such as test strips), and damage the control of illegal drugs

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