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Immobilized microbial inoculum for remediating PAHs contaminated soil and preparation method thereof

A contaminated soil immobilization technology, applied in the field of remediation of polycyclic aromatic hydrocarbons contaminated soil, can solve the problems of remediation of contaminated soil that have not been reported, and achieve the effect of fast adsorption speed, good effect and simplified process

Inactive Publication Date: 2010-06-09
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been some successful examples of using IMR technology to remediate polluted water bodies, but remediation of polluted soil has not been reported

Method used

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  • Immobilized microbial inoculum for remediating PAHs contaminated soil and preparation method thereof
  • Immobilized microbial inoculum for remediating PAHs contaminated soil and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0025]The method for immobilizing microorganisms by using the by-products of agricultural crops as a carrier comprises the following steps: firstly, seed liquid is prepared, and the carrier is treated at the same time; and then the mixed bacterial strains are adsorbed, fixed and multiplied in a solid medium. Specific steps are as follows:

[0026] 1) Preparation of bacterial solution:

[0027] The strains Gordona sp., Arthrobaccer sp., Alcaligenes sp., Ochrobactrum, Psedomonas sp., Bacillus sp. .), mycobacterium (Mycobacterium sp.), (all purchased from the microbial strain bank of Shenyang Institute of Applied Ecology, Chinese Academy of Sciences, which is open to the outside world and anyone can purchase; meanwhile, the strains used can be purchased on the market) The above bacterial classifications were inoculated in 90ml of enrichment medium containing PAHs pollutants in the ratio of 1:1:1:1:2:3:2 in parts by weight, and then put into 10g sewage irrigation area (Sanbaotun,...

Embodiment 2

[0036] Embodiment 2 differs from Embodiment 1 in that:

[0037] 1) Preparation of bacterial solution:

[0038] The bacterial species are Gordona sp., Arthrobaccer sp., Alcaligenes sp., Ochrobactrum, Psedomonas sp., Bacillus sp. .), mycobacterium (Mycobacterium sp.) Inoculate the above bacterial species in 100ml of enrichment medium containing PAHs pollutants in the ratio of 0.5: 1.5: 0.5: 1.5: 3: 4: 3 in parts by weight , and then put 15g of aged PAHs-contaminated soil in the sewage irrigation area, and shake it in a constant temperature shaker at 28°C and 130rpm / min for 5 days, and set it aside for use.

[0039] The enrichment medium containing PAHs pollutants is as follows: 1.25ml PAHs solution is added in a sterile 500ml Erlenmeyer flask, so that the total concentration of polycyclic aromatic hydrocarbons in the enrichment medium is 100mg / L; , and then pour the aliquoted 100ml sterile enrichment medium into the Erlenmeyer flask. The enrichment medium is calculated by wei...

Embodiment 3

[0047] Embodiment 3 differs from Embodiment 1 in that:

[0048] The preparation method of the immobilized bacterial agent for remediating PAHs polluted soil, enrichment, cultivation and domestication: first put Gordonia, Arthrobacter, Alcaligenes, Paleobacterium, Pseudomonas, Bacillus and Mycobacterium by weight The number of parts counted as 0.5: 0.5: 0.5: 0.5: 2: 3: 2 was inoculated in 50 ml of enrichment medium containing PAHs pollutants, and then the PAHs contaminated soil of 12% by weight of enrichment medium was put in, Shake culture at 29°C, 125rpm / min in a constant temperature shaker for 6 days, and set aside.

[0049] The enrichment medium containing PAHs pollutants is: adding PAHs solution into the enrichment medium so that the total concentration of polycyclic aromatic hydrocarbons is 150 mg / L. The enrichment medium is calculated by weight percentage: glucose 0.15%, peptone 0.08%, yeast extract 0.08%, NaCl 0.1%, NH 4 NO 3 0.8%, K 2 HPO 4 0.3%, MgSO 4 ·7H 2 ...

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Abstract

The invention relates to remediation technology of polycyclic aromatic hydrocarbon (PAHs) contaminated soil, in particular to an immobilized microbial inoculum for remediating the PAHs contaminated soil and a preparation method thereof. The preparation method specifically comprises the following steps of: inoculating a prepared microbial mixed strain liquid into a solid medium, namely a processed microbial inoculum vector; culturing the microbial mixed strain liquid at a constant temperature of between 28 and 30 DEG C in an incubator for 3 to 4 days; and carrying out adsorption immobilization and proliferation to prepare an immobilized mixed microbial preparation. The method specifically adopts crop byproducts as the vector, and has the advantages of high adsorption speed of mixed strains, good proliferation effect, simple operation, no secondary pollution, rich sources of the crop byproducts and low price. The prepared immobilized cell granules are put into the PAHs contaminated soil in a percentage of 2 to 4 percent, which can effectively remove PAHs contaminants in the soil. The degradation rate of the PAHs processed by 70d can reach 74 percent when the concentration of the PAHs in the contaminated soil is 84mg / kg.

Description

technical field [0001] The invention relates to the restoration technology of polycyclic aromatic hydrocarbons (PAHs) polluted soil, in particular to an immobilized bacterial agent for repairing PAHs polluted soil and a preparation method thereof. Background technique [0002] Conventional environmental remediation technologies mainly refer to chemical remediation, physical remediation, ecological remediation and biological remediation. The latter two are often classified as unified technologies, and in-situ methods are generally adopted in engineering. As an emerging technology with low energy consumption and no secondary pollution, bioremediation technology is suitable for large-scale light or moderately polluted soil treatment. It has the advantages of low cost, easy operation, and no pollution to the environment, and can improve soil and improve soil fertility. , so it has a wide range of applications and development prospects. [0003] Since the 1990s, my country has c...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/14B09C1/10C12R1/32C12R1/07C12R1/38C12R1/05C12R1/06C12R1/01
Inventor 巩宗强李晓军鞠京丽赵青董殿波张春桂张海荣
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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