549 results about "Morchella" patented technology

The invention discloses a culturing method for sporocarp of Morchella. The culturing method comprises the following steps: fungal strain preparation, fungal strain culturing, soil treatment, sowing, covering and temperature preservation, management in the growth period of mycelia, fruiting acceleration treatment, management in a fruiting period, and harvesting. According to the invention, operation steps like drawing of fungal strains from a fungal strain bottle or a fungal strain bag, fungal strain hydration, and placement of a large amount of nutrition material bags on the surface of soil in a traditional technology are removed, so the field-cultivation or indoor-cultivation technique for the Morchella is more simple and convenient; the cost of the nutrition material bags is reduced by three fourth or more; the generation cost of cultivation of the Morchella is greatly reduced; the technical difficulty of the cultivation of the Morchella is greatly decreased; and economic benefits are greatly improved.

The invention discloses a preparation method for an edible fungus flavor food. The edible fungus can be any one from edible agaricus bisporus, straw mushrooms, oyster mushrooms, shii-take, needle mushrooms, pleurotus nebrodensis, pleurotus eryngii, agrocybe aegerita, hypsizigus marmoreus, agaricus pratensis schaeff, spring mushrooms, stropharia rugoso-annulata, sparassis crispa, macrolepiota procera, tremellodon gelatinosum, black trumpet mushrooms, hericium erinaceus, pholiota nameko, lepista personata, mitake mushrooms, lactarius hatsudake, golden oyster mushrooms, pholiota adiposa, cantharellus cibarius, termitomyces albuminosus, tricholoma giganteum, pleurotus citrinopileatus, calocybe gambosa, truffle, amillariella mellea, fungus suillus, beef-steak fungus, lactarius deliciosus, tricholoma matsutake, agaricus bitorquis, pleurotus pulmonarius, toadstool and russula vinosa, and after treatment, the edible fungus is steeped into a steeping liquor prepared from red wine vinasse, high quality dry red wine and a flavouring agent in a vacuum and normal temperature and normal pressure combined condition, so as to obtain the flavor food; the edible fungus flavor food for leisure is prepared through oil-bath dewatering; a flavor food flavor puffed food is obtained through a swelling process, so as to meet consumers' hobbies and requirements.

The invention belongs to the field of morchella cultivation, and particularly relates to a method for cultivating morchella. The method is characterized by comprising steps of firstly, performing field preparation: spacing ridge compartments and ditching; secondly, sowing the morchella and wheat: broadcasting 75-150Kg per mu of strains of the morchella on compartment surfaces, covering a soil layer on each compartment surface, applying compound fertilizers after the strains of the morchella are watered, broadcasting 2-5Kg per mu of wheat on the compartment surfaces in 1-10 days after the compound fertilizers are applied, covering a sunshade net on the compartment surfaces, and watering the strains of the morchella and the wheat every 2-3 days; thirdly, applying morchella growth promoters: applying the morchella growth promoters in the compartment surfaces in 35-45 days after the wheat is sowed; fourthly, performing fruiting and harvesting field management for the morchella: shading and watering the strains of the morchella and the wheat; and fifthly, performing field management for the wheat: weeding and applying additional fertilizers. The width of each compartment surface ranges from 90cm to 110cm, the width of each ditch ranges from 30cm to 40cm, the depth of each ditch ranges from 20cm to 30cm, and the humidity of each compartment surface keeps within the range from 60% to 80% in the fourth step. The method has the advantages of low cost, high and stable yield, simplicity in operation and easiness in popularization.

The invention discloses a method for preparing an efficient probiotic preparation from macrofungi bacterial liquid (mycoplasm). The method comprises the following steps: preparing a liquid or a solid culture medium from agricultural and sideline products and culturing fungi to obtain a bacterial liquid or a mycoplasm; carrying out physical and biological treatment on the bacterial liquid or the mycoplasm to enable polysaccharide polypeptide substances to be released from cells and adding a nitrogen source and a carbon source to prepare a probiotics culture medium; and then inoculating the probiotics and culturing to obtain the efficient probiotic preparation. The fungi comprise medicinal (edible) fungi of lentinu edodes, agrocybe cylindracea, morchella, pholiota nameko, hericium erinaceus, bolete, pleurotus nebrodensis, pleurotus citrinopileatus, lucid ganoderma, coriolus versicolor, cordyceps taishanensis, poria cocos, bamboo fungus, lactarius deliciosus and the like; and the probiotics is one or more selected from bacillus, saccharomycete, lactobacillus, enterococcus, bifidobacterium and the like. The probiotic preparation prepared by the method disclosed by the invention is rich in active substances, such as polysaccharides and polypeptides, has better health and treatment effects that those of a conventional probiotic preparation and can be used as therapeutic drugs and health foods or feed additives of human and animal gastrointestinal diseases.

The invention belongs to the technical field of edible mushroom cultivation, in particular relates to a method for cultivating morchella, and aims to solve the technical problem of providing a novel method for cultivating morchella. The main improvement is that the method is achieved by adopting a cultivation mode and a novel cultivation medium in match. The method provided by the invention comprises the following steps: A, pretreating a cultivation land; B, opening ridges, and putting morchella bags on the ground; C, covering the morchella bags with soil, and constructing the ridges; D, growing the morchella; E, placing nutrient bags; and F, inducing the morchella till the morchella is harvested. By adopting the method provided by the invention, the cost of each mu of the land can be reduced, moreover, the yield of the morchella can be remarkably increased, and the method is worthy of popularization and application.

A method for reproduction of morel includes such steps as choosing proper field in forest or others, removing harmful plants, sowing, conventional management, and picking up. Its advantages are high yield and high quality.

The invention provides a method for using raw material to cultivate morchella. Specifically, a mushroom house with 30% of sunny areas and 70% of sunless areas can be built on the place where soil properties are loose and moist and water is discharged well; corncobs, bits of wood, bran, chaff, bean flour, plant ash, phosphatic fertilizer, gypsum and sugar are utilized as the raw material; cultivation pits are dug in the mushroom house, two layers of the material are laid in each cultivation pit, two layers of strain are scattered in each cultivation pit, and soil covering is conducted; finally, air humidity in the mushroom house keeps ranging from 85% to 95%, temperature ranges from 5 DEG C to 16 DEG C, and sporocarps can be formed in about one mouth in the environment. By means of the method, the defect that mass commercialization artificial cultivation of the morchella is not achieved so far is overcome effectively. Meanwhile, the produced morchella is cultivated in a wild imitating mode, and therefore entities of the morchella are excellent. In addition, when the method is implemented, the raw material is directly used for cultivation, the production process does not have the procedures of bagging, sterilizing and disinfecting, time and labor are saved, and work efficiency is greatly improved.

The invention discloses an efficient planting method of morchella in a high-cold and high-altitude area. The method comprises steps as follows: 1, separate cloning of morchella fruiting bodies; 2, preparation of a cultivation strain; 3, preparation of a foreign nutrition bag. The method is not limited by natural conditions in areas with harsh natural conditions, such as high-cold areas, high-altitude areas and the like, morchella can be planted on a scale in a standardized manner annularly, sources of used materials are wide and convenient, waste can be turned into valuable, and life and production waste such as waste paper, waste cartons, plant ash, crop straw, fallen leaves, composted poultry, livestock manure and the like are recycled. By means of the technology, the fruiting repeatability is high, the yield is high and stable, and the technology is an economical and environment-friendly technology for out-of-season planting of morchella in a solar greenhouse.

The invention relates to a spire morchella strain screening and bacteria species manufacturing method. It belongs to microorganism technique. The strain morchella conica M0503 is preserved at Chinese microscobial preservation management committee common mircens on August 11th, 2005 year. Its preservation number is CGMCC NO.1437. The formed liquid and solid bacteria species can be used to protect morchella wildlife resource, and increase its nature yield and quality. Thus it has great social, economic, and ecological benefits and broad application prospect.

The invention relates a bionic cultivation method of Morchella, which has a cultivation procedure of mother seed making procedure, original seed making procedure, cultivation seed making procedure, germination cultivation procedure, sclerotium cultivation procedure, sporocarp appearance processing operation procedure, product harvest procedure. The formula of the cultivated seed making procedure is that a material mixture of 80% wheat, 18% wheat bran, 1% gypsum, 1% glucose, 1:1 water, is mixed and stirred with a mixed peat soil, occupying 30% of the total weight, of 96% peat soil, 4% wood ash, and 30% water. The process of the sclerotium cultivation procedure is: a plastic container full of myculium in germination cultivation procedure is cultivated in a condition of 16-18 DEG C, light avoiding, 90%-95% air humidity for about 25 days, and if a concentration of CO2 is over 1.5%, ventilation and air exchange is done per hour. Manual cultivation of Morchella will be performend successfully by using the technology in factory production.

The invention provides a cultivation method of morchella, and belongs to the technical field of edible fungus cultivation. The cultivation method comprises the steps of mother fungus cultivation, stock culture, cultivation inoculation and indoor cultivation management. According to the cultivation method, by controlling a culture medium, the temperature, the time and other coefficients in different periods, the production cycle of the morchella can be shortened, sundry fungus contamination is reduced, and the yield of the morchella is increased; in addition, through a distillers' grain raw material added in a stock culture medium and a cultivation culture medium, sundry fungus growth can be inhibited while the quality of the morchella is guaranteed, and the success rate that the morchella grows on fungus sticks reaches up to 92% or above.

The invention provides an industrialized cultivation method of Morchella importuna. The method is characterized by comprising steps as follows: (1) preparation of a culture substrate; (2) preparation of a liquid strain; (3) mycelium culture; (4) fruiting management: mycelia in the step (3) are transferred to a fruiting room for fruiting management, and the fruiting management comprises management in a primordium formation promoting period, management in a mushroom bud formation period, management in a growth period and management in the manure period. According to the method, crop waste is taken as a raw material for preparation of a culture medium, liquid strains are used for sowing, industrialized cultivation of Morchella is completely realized indoor, the method is simple to operate, low in cost, short in cycle, controllable in quality and capable of realizing industrialized production all year round, and one pollution-free, pleasantly scented and delicious green product is obtained.

The invention discloses a process for manufacturing high-quality morchella strains. The process includes selecting acquisition time of morchella; screening high-quality strains; cultivating mycelia; manufacturing and cultivating mother strains; manufacturing and cultivating cultivated strains. The morchella is acquired in the seventh-tenth days when first or second crops of morchella are fruited in February or March of the second year of morchella planting under the conditions that morchella individuals are longer than or equal to 10cm and are shorter than or equal to 15cm, pilei of the morchella individuals are plump, ridges and pits on the pilei are completely spread and stipites of the morchella individuals are milky; the high-quality strains are ejected and screened step by step according to forms and colors of the pilei, colors and forms of the stipites, colors and quantities of spores and sclerotium growing ability of the strains. The process has the advantages that the yield of fresh morchella which grows from the high-quality morchella strains manufactured by the aid of the process is stable for three continuous years and is stabilized at about 80kg, the fruiting time is stable, the growing speeds of the individuals are similar to one another, and the technical problem of instable yields in current morchella industrial development can be solved.

The invention provides a manufacturing method for morchella mother culture. The method is characterized by comprising the following steps of: 1, boiling 1 to 3 parts of fresh insect-free populus bonatii smashed branches or populus bonatii leaves in 10 parts of water based on a mass ratio for 25 to 30 minutes, filtering the mixture, and taking juice to prepare populus bonatii leachate; 2, mixing the populus bonatii leachate and PDA culture solution in a mass ratio of 1: 1, adding 0.2 percent of Na3PO4.12H2O, 0.2 percent of KH2PO4, 0.05 percent of MgSO4 and 0.005 percent of VB1 based on the mass percent of the mixed liquor, uniformly stirring the mixture, and sterilizing the mixture at the temperature of 121 + / 1 DEG C for 30 minutes to prepare a culture medium; and 3, cooling the culture medium, inoculating the morchella mother culture, culturing the inoculated culture medium at the constant temperature of 23 DEG C for 10 to 12 days, and culturing the culture medium in an incubator at the temperature of between 8 and 12 DEG C for 4 to 6 days. The manufacturing method has the advantages of strong hypha, strong twisting force, and easy formation of sclerotium on protospecies or cultivar.

The invention relates to a cultivation and application method of poplar woods special for morchella, and belongs to the technical field of edible fungi planting. The cultivation and application methodincludes selecting poplar strains which sprout early in spring and defoliate late in autumn; selecting fertile sandy soil, with the pH value of 5-7.5, as soil for planting plots; planting poplars onthe planting plots according to the planting spacing of 3*4m or 4*4m, taking each 20mu as a planting area, arranging an operation road between every two adjacent planting areas, and cultivating the poplars for more than 3 years. The planting land special for the poplar woods is subjected to earth-up for morchella planting in every autumn. The cultivation and application method has the advantages that proper species, planting density, modification modes and under-forest morchella planting methods are selected based on existing poplar species; the morchella is planted every autumn after earth-up, so that yield of the morchella is increased, high product quality is achieved, and green food requirements are met.

The invention discloses a health-preserving edible fungi composition. The health-preserving edible fungi composition comprises main materials and auxiliary materials, wherein the main materials comprise stropharia rugosannulata, lentinula edodes, tricholoma quercicola zang, yellow boleti, white boleti and tricholoma matsutake fungi; the auxiliary materials comprise morchella, tricholoma matsutake fungi, yellow boleti, white boleti, tricholoma quercicola zang, stropharia rugosannulata, termitomyces albuminosus, red boleti, agrocybe cylindracea, lentinula edodes, catathelasma ventricosum, sarcodon aspratus, black boleti, coral fungi, edible salt, white granulated sugar, maltodextrin and spices. According to the health-preserving edible fungi composition, the tastes of the lentinus edodes and the yellow boleti are taken as principal edible tastes; nutritional ingredients of the tricholoma quercicola zang, the white boleti, the stropharia rugosannulata and the tricholoma matsutake fungi are combined with those of the lentinula edodes and the agrocybe cylindracea, so that the health-preserving edible fungi composition is rich in proteins, trace elements and amino acids required by a human body and other elements beneficial to the human body; moreover, the mouth feels of various fungi are fused to meet the taste demands of the public. The edible salt, the white granulated sugar, the maltodextrin and the spices serve as common condiments for cooking food to improve the mouth feel of soup or food and meet demands of the public on the mouth feel.

The invention relates to a morchella sunlight greenhouse bionic environment high-yield cultivation method, which comprises the following steps of (1) preparing a liquid culture medium; (2) performing morchella stem removal on strongly growing morchella sporocarps with great size and good growth condition in wild environment; sanitizing rest pileuses and then performing slicing; inoculating the morchella in the liquid culture medium for culture to obtain a mother culture medium; (3) preparing a cultivation culture medium; (4) injecting the mother culture medium in each bag of cultivation culture medium for inoculation beside the flame of an alcohol lamp; (5) performing morchella fermentation after the inoculation completion, and obtaining white hyphae after 15 days; (6) selecting a large-span upright-post-free sunlight greenhouse, maintaining the soil pH value to 7 to 9, and meanwhile, enabling the soil humidity to be 30 to 40 percent; (7) performing ridging seeding; (8) performing morchella fermentation management; (9) performing harvesting: when the morchella covers grow to 4 to 7cm, lamellae on the morchella cover gradually expand, the harvesting can be performed. The method has the advantages that the operation is simple; the large-area demonstration and promotion is easy; high-yield and efficient cultivation is realized through whole-process bionic environment simulation.

The invention discloses a Morchella cultivation material, which comprises the following components by weight: 25-35 parts of corn stalk, 25-35 parts of poplar sawdust, 20-30 parts of rape straws, 10-20 parts of peanut shell, 15-25 parts of sunflower seed meal, 8-15 parts of coconut shell, 3-6 parts of chestnut shell, 7-12 parts of Momordica grosvenori dregs, 5-8 parts of vinasse, 3-6 parts of Chinese Honeylocust Fruit shell, 4-7 parts of Gentiana apiata, 3-6 parts of dandelion and 2-5 parts of honeysuckle and the like. The method for cultivation comprises the following steps: (1) preparation of the Morchella cultivation material, (2) seed mixing, (3) seed sowing, (4) mycelial growth management and (5) fruitbody growth management. The waste raw materials are used, and scientifically and reasonably matched, nutrients are fully complementary, the Morchella cultivation material is environmentally-friendly and economic, herbal ingredients are added, the beneficial functions of Morchella are improved, the Morchella cultivation material has antibacterial and antiviral effect; the method for cultivation is simple and practical, low-cost and high-yield, and quality is good.

The invention discloses a preparation method of a solid nutrient solution soil covering material special for cultivating morchella esculenta, belonging to the field of soil covering materials. The preparation method comprises the steps of mashing garlic, onions and fresh ginger, placing the mashed garlic, onions and fresh ginger into a fermentation tank, carrying out high-temperature sterilization firstly, then, inoculating a yeast to ferment in a dark place, filtering after fermenting, and preparing a disinfectant from the filtrate; then, mixing filter residues, guano and fishbone meal to obtain a soil covering substrate; incinerating corn stalks, mixing ash and the disinfectant, and stirring; mixing after adding white vinegar, and then, concentrating to obtain a black mud-like material; and then, mixing the black mud-like material and the prepared soil covering substrate, and drying to obtain the special solid nutrient solution soil covering material for cultivating morchella esculenta. The preparation method is used for producing the solid nutrient solution soil covering material; the morchella esculenta can still grow without covering original surface oil with morchella esculenta, so that morchella esculenta is produced on a large scale, and the problem of low yield of morchella esculenta is solved fundamentally; and the preparation method is simple in production process and low in cost.

The invention discloses a flavored morchella raw fish which is prepared from the following raw materials in parts by weight: 90-110 parts of frozen tuna, 15-16 parts of lyophilized royal jelly powder, 22-24 parts of grape pulp, 8-9 parts of morchella, 5-7 parts of steamed bun, 9-11 parts of mango juice, 17-18 parts of walnut kernel, 7-8 parts of honey, 0.5-1 part of a mulberry root, 0.4-1 part of penthorum Chinese pursh, 1.8-2 parts of wax gourd seeds, 0.2-0.3 part of sodium glutamate, 0.1-0.3 part of ethyl maltol, 0.1-0.2 part of phosphate, 1-2 parts of lactic acid bacteria, 300mmol / L of CaCl2 solution, refined salt and a proper amount of sugar. The flavored morchella raw fish has the advantages of unique taste, long shelf life and good health effect; the flavored morchella raw fish is unfrozen by a pickling method and is pickled by an injection method; the technology is unique and novel; the value-added space and the extension benefits of the raw fish product are increased; and due to the addition of traditional Chinese medicine components such as mulberry root and penthorum Chinese pursh, walnut kernel is endowed with the health effects of moistening the lung for removing phlegm and clearing away heat and toxic materials.

The invention provides an edible fungus mycelium fermented drink and a making method thereof. The edible fungus mycelium fermented drink is made by inoculating an edible fungus strain into fermentation liquor and then performing fermentation. The fermentation liquor is mainly prepared form water, plant fruits, potatoes, glucose, rock candy, peptone, the vitamin B1 and edible soil, wherein the plant fruits include any one or more of red dates, blueberries, wolfberry fruits and Paris polyphylla fruits, and the edible fungus includes any one of morchella, collybia albuminosa, bolete, tricholoma matsutake and truffles. The edible fungus mycelium fermented drink is of great benefit to human health as rich nutritional ingredients of the plant fruits and edible fungus mycelia are contained, is high in nutritional value as no substance harmful to the human body is added, and is low in price; as the making method is simple and convenient to implement, and the raw material cost is low, the investment of the whole production line is small.

The invention discloses a high yield method for cultivating morchella in a forest, which comprises the following steps: 1, selecting a forest land; 2, preparing the land; 3, sowing; 4, managing the forest after sowing; 5, preparing a foreign nutrition bag; 6, using the foreign nutrient bag; 7, overwintering, fruiting and managing in early spring; 8, harvesting. According to the method, the morchella, which is cultivated by using jujun grass as the mushroom bed nutrient material, has large grain size and thick meat, the taste is delicious, the yield is considerable, and the problem of waste ofthe space in the forest is solved, and the land resource is reasonably utilized.

The invention relates to an enzyme composition with the efficacy of strengthening kidney and tonifying Yang. The enzyme composition is prepared from the following components: 1 to 15 weight parts of probiotics, 10 to 120 weight parts of enzyme combination stoste and 10 to 80 weight parts of traditional Chinese medicinal extract; the enzyme composition is formed by treating the traditional Chinese medicinal extract by a sterilization process, then mixing the treated traditional Chinese medicinal extract with the enzyme combination stoste, inoculating a probiotic group and performing airtight fermentation. The traditional Chinese medicinal extract is prepared by extracting the following crude drugs in parts by weight: 2 to 10 parts of rhizoma dioscoreae, 2 to 10 parts of sea cucumber, 1 to 8 parts of morchella, 2 to 10 parts of rhizoma imperatae, 2 to 8 parts of chenopodium quinoa, 1 to 8 parts of root or fruit of fourpetal rose, 0.5 to 6 parts of sea horse, 0.5 to 6 parts of rhizoma curculiginis, 0.2 to 3 parts of sunflower stem pith, 0.5 to 6 parts of fructus jujubae and 0.5 to 5 parts of radix notoginseng. According to the enzyme composition, the probiotics and functional oligosaccharide are combined for use, and traditional Chinese medicinal components are added; the enzyme composition has the efficacy of strengthening kidney and tonifying Yang, and benefiting essence and tonifying Qi.

The invention discloses a large-scale cultivation method of morchella. The large-scale cultivation management technology of morchella belongs to the technical field of edible fungi cultivation. The method comprises the following steps of cultivation season selection, plot selection, cultivation greenhouse establishment, land preparation, compartment opening, bedding, seeding, earthing, external assistance nutrition bag placement, hypha cultivation management, fruiting management and harvesting. The large-scale cultivation management technology of morchella has the advantages that the technology is scientific and reasonable, the operation is simple, the promotion is easy, and the morchella cultivation can be promoted to be more standard and scientific, thereby increasing the repeatability of artificial cultivation of morchella, providing guarantee for high yield and stable production, and improving the economic benefits of the artificial cultivation of morchella.

The invention discloses a preparation method of producing morchella five grains nutritious paste with corn, oat, soybean, millet and sorghum rice as culture medium. The morchella is a well-known wild edible fungus, has good effects on kidney deficiency, dizziness and insomnia, stomach inflammation and weakness of the spleen and the stomach and has the efficacy of body building, preventing cold and enhancing body immunity. The adopted morchella strains come from the wild morchella of Guandi Mountain, Shanxi Province. The morchella strains grow well on the pure solid culture medium of corn, oat, soybean, millet and sorghum rice, the five grains culture medium is inoculated in the morchella strain after cleaning, infusion, bottling and sterilization, and the finished product is obtained through culturing, taking out, drying, crushing and sieving packaging. For the product, the novel health-care food or the convenient food, the instant food and the like can also be developed according to different demands.

The invention belongs to the field of toadstool cultivation, and in particular relates to a toadstool nutrition bag prepared by utilizing edible mushroom dregs and a preparation method thereof. The invention has the main improvement that the edible mushroom dregs are adopted as main raw materials, and are soaked by utilizing a liquid cultural substance of chryseobacterium HT2. The preparation method comprises the following steps of (1) collecting the mushroom dregs; (2) mixing the mushroom dregs and the liquid cultural substance of the chryseobacterium HT2, and soaking in an open vessel; (3) filtering to obtain the mushroom dregs, adding 1 to 6 percent of unslaked lime and 2 to 10 percent of husk or wheat bran, mixing to obtain a mixture, bagging, and sterilizing to obtain the nutrition bag. According to the method provided by the invention, the preparation raw materials of the nutrition bag are changed, the mushroom dregs remained after cultivating the edible mushrooms are adopted to replace traditional wheat berries and husks, and the chryseobacterium HT2 is utilized to ferment and decompose the mushroom dregs, so that cellulose and hemicellulose in the mushroom dregs are partially turned into saccharides easily utilized by the toadstool; agricultural wastes are utilized, so that the cost is reduced, the pollution is reduced, and the yield of the toadstool is improved.

The invention belongs to the technical field of edible fungi cultivation and particularly relates to a saline-alkali soil morchella culturing method. The saline-alkali soil morchella culturing methodis implemented through the following steps of (1) seed fungi selection, (2) seed fungi preparation, (3) cultivation facility preparation, (4) ground processing, (5) sowing and nutrition supplementation, (6) construction of small greenhouses, (7) fruiting and harvesting management and (8) fungi residue returning. By means of the saline-alkali soil morchella culturing method, the adaptability of mycelia can be enhanced; a three-layer greenhouse culture mode is applied for the first time to facilitate temperature control and shorten the culture time; supplementation of culture mediums facilitatesrapid growth restoration of the mycelia; an applied bag arranging manner can simplify culture processes and save labor and material costs. The saline-alkali soil morchella culturing method can effectively reduce the salinity content of soil; fungus growth can improve the soil structure, avoid re-salinization and improve the pH environment of soil; nutrient bags are returned to fields to improve the content of organic matters in soil and accordingly improve the fertility of soil, the land utilization rate as well as the economic benefits of morchella.

The invention provides a plateau morchella planting method and relates to the technical field of fungus crop planting and cultivating methods. The plateau morchella planting method comprises following steps: disinfection of a planting area, ash substance preparation, fermentation of a basic material, preparation before planting, building of a planting greenhouse, primary planting, secondary inoculation, planting management, topdressing and picking. The plateau morchella planting method has the beneficial effects as follows: by means of land disinfection, interference microorganisms can be effectively reduced, so that the ecological effect after forest fire is simulated; for ash substance preparation, a soil texture surface structure after forest fire is simulated, ash substances can enter soil through two times of mutually crossed rotary tillage, and a planting effect better than that of a natural environment is realized. A fermented basic material simulates a vegetational branch and leaf enrichment state in the later period of the morchella growing environment, so that the simulated ecological planting environment is created better, and the yield is increased. Because of planting in the greenhouse, the morchella can be sown in spring and autumn, the time limitation is broken through, and the crop production cycle is shortened.

The invention discloses a cultivation planting method for artificial domestication of wild morchella esculenta. The cultivation planting method for artificial domestication of wild morchella esculentacomprises the steps of 1 harvesting wild morchella esculenta, 2 performing processing, 3 preparing soil, 4 sowing seeds, 5 performing daily management and 6 performing harvesting. The influence of geographic environment on planting is reduced, dependence on the climate environment is lowered, the yield of morchella esculenta is increased, the quality is obviously improved, 95% of morchella esculenta can reach the good quality, and the activity of the wild morchella esculenta is ensured from the picking source, so that mother strains of the morchella esculenta are more easily obtained, the success rate is high, the purpose of artificial domestication of the wild morchella esculenta can be achieved, anti-season output of the morchella esculenta is ensured, the planting method is simple, andthe survival rate of the morchella esculenta is increased.

The invention relates to a method for distinguishing mating types of four varieties in black morchella group and a primer pair, and belongs to the field of biotechnology. The method comprises the following steps: extracting the DNA of a strain to be detected as a template, performing PCR amplification through the primer pair P7-2F / P7-2R or P8-5F / P8-5R to obtain a strain of MAT1-1 mating type of an expected band, performing PCR amplification through the primer pair P10-1F / P10-2R or P10-2F / P10-3R to obtain a strain of MAT1-2 mating type of an expected band, wherein the strain which can be detected by the two expected amplification bands is of two mating types. The four primers can be general to the mating type detection of the four varieties of morchella. A detection result of the method is high in specificity, and the method is stable, reliable and quick, and is applicable to researches on selection, cultivation, mating type genes and system development of strains of the morchella.