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2282results about How to "Good yield" patented technology

Methods and Compositions for Mammalian Cell Lines for Transfection and Protein Expression in Serum-Free Medium

Disclosed are compositions and methods for increasing the longevity of a cell culture and permitting the increased production of proteins, preferably recombinant proteins, such as antibodies, peptides, enzymes, growth factors, interleukins, interferons, hormones, and vaccines. Cells transfected with an apoptosis-inhibiting gene or vector, such as a triple mutant Bcl-2 gene, can survive longer in culture, resulting in extension of the state and yield of protein biosynthesis. Such transfected cells exhibit maximal cell densities that equal or exceed the maximal density achieved by the parent cell lines. Transfected cells can also be pre-adapted for growth in serum-free medium, greatly decreasing the time required to obtain protein production in serum-free medium. In certain methods, the pre-adapted cells can be used for protein production following transfection under serum-free conditions. In preferred embodiments, the cells of use are SpESF or SpESF-X cells.
Owner:IMMUNOMEDICS INC

Catalytic hydroconversion of chemically digested organic municipal solid waste materials

A hydrocarbon liquid feedstock containing at least 50 wt. % chemically digested organic-MSW material is catalytically hydroconverted utilizing either a single stage or two-stage catalytic reaction process to produce desirable lower-boiling hydrocarbon liquid products. The catalyst can be either a particulate supported type catalyst such as containing cobalt and / or molybdenum and / or nickel on alumina support, or a dispersed slurry type catalyst containing mainly iron oxide with anions of molybdate, phosphate, sulfate or tungstate, and combinations thereof. Broad useful reaction conditions are 600-860° F. (315-460° C.) temperature, 1000-3000 psi hydrogen partial pressure, and fresh feed rate of 20-60 pounds / hr / ft3 reactor volume. Effluent material from the final stage catalytic reactor is phase separated and the resulting liquid portion is fractionated to produce the desired low-boiling hydrocarbon liquid products particularly useful as transportation fuels. If desired, the chemically digested organic-MSW feedstock can be blended with petroleum residua and / or particulate coal and / or mixed waste plastics and the blended feed material processed in catalytic two-stage reactors to produce similar desirable low-boiling hydrocarbon liquid products.
Owner:HYDROCARBON TECH

Regiospecific synthesis of rapamycin 42-ester derivatives

A method for the regiospecific synthesis of rapamycin 42-ester derivatives is described. The method involves lipase-catalyzed acetylation of 42-hydroxy of rapamycin with an acyl donor such as a vinyl ester, an isopropenyl ester or an anhydride in a suitable organic solvent.
Owner:WYETH

Method for tissue-culture breeding and wild-simulated cultivation of dendrobium candidum

The invention discloses a method for tissue-culture breeding and wild-simulated cultivation of dendrobium candidum, which comprises: inoculating dendrobium candidum tender straw slices onto a culturemedium for culture under conditions of a temperature of 22 to 24 DEG C and an illuminance of 1,000 to 1,500 Lx to first induce the growth of calles to form protocorms and then promote the multiplicative growth of the protocorms into 5 to 8 centimeter strong seedlings; and moving the seedlings out of a culture room, hardening the seedlings at a normal temperature for 3 to 5 days and transplanting the seedlings into the ground in a green house. The plane basic layer of a cultivation check field in the green house are provided with spaced breather holes; the upper layer of the cultivation check field is a cultivation medium; the seedlings are planted at a row spacing of 12*8cm and a spacing in the row of 12*8cm; in the green house, the temperature is 0 to 28 DEG C, the humidity is 60 to 70 percent and the shade density is 70 to 80 percent; and disease and pest prevention and control are carried out periodically and the dendrobium candidum can be harvested in 3 to 4 years. The method improves the civil year breeding coefficient of the dendrobium candidum by about 10 times, provides enough seedlings for large-scale cultivation, solves the problem of wild-simulated cultivation and realizes scale cultivation.
Owner:HANZHONG PLANTS RES INST +1

Integrated circuit wafer packaging system and method

InactiveUS20050269241A1Eliminate any forceEliminate and seriously minimize motionInternal framesSemiconductor/solid-state device manufacturingQuality assuranceIntegrated circuit
A packaging system, hereinafter referred to as the Critical Packaging System, relates to critical issues that associate with sensitive articles such as IC wafers before, during and after shipment phases. The system employs a choice of two or more specialty designed containers, and any one selected design having choices of two or more methods by which to avoid, reduce and / or eliminate wafer damage from breakage, scratches and / or corrosion during shipment phases. For the purpose of maximizing product yield during packaging phases a special apparatus is used to insert wafers within containers without scratch damage. The following programs are used in packaging: (1) Quality Assurance / Certification, (2) Critical Factor Monitoring, and (3) a Recycle and Refurbish Program. These programs are specifically designed to achieve new levels of product yields, reduce product cost, and landfill impact.
Owner:CONVEY INC
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