The invention discloses a transparent conduction film, a conduction structure thereof and a preparation method of the transparent conduction film. The transparent conduction film is of a single-sided dual-layer conduction structure. The conduction structure comprises a first metal embedding layer and a second metal embedding layer, wherein the first metal embedding layer is formed by impressing on a substrate or impressing on a polymer layer on the surface of the substrate; and the second metal embedding layer is formed by coating the polymer layer on the surface of the first metal embedding layer through impressing, the first layer of conduction structure and the second layer of conduction structure have a grid groove structure, and conductive materials are filled in grooves. The single-sided graphical transparent conduction film provided by the invention has the advantages of high resolution and transmittance, independent and adjustable sheet resistance, and the like. According to the transparent conduction film, the cost can be reduced, and the weight and the thickness of a manufactured touch panel can be reduced.