79 results about "Trametes" patented technology

Compositions for treating skin containing at least one extract from the genus Trametes and at least one viscosity enhancing synthetic polymer, and methods for whitening skin, treating skin for improvement, or treating skin disorders associated with pigmentation such as uneven pigmentation, skin mottling, or age spots or other dermatological disorders such as eczema, rosacea, hyperkeratinization, and so on.

The invention relates to an organic fertilizer and a production method thereof, and particularly relates to a method for producing an organic fertilizer by utilizing hickory hulls. The method comprises the following steps of: uniformly mixing hull powder, meal and superphosphate, inoculating the obtained mixture into a microbial inoculan I after moisture is adjusted, and carrying out aerobic fermentation under the turning condition; after fermentation, inoculating a microbial inoculan II into the material, and then carrying out piled aging, drying, crushing, sieving and packaging and the like to obtain a hickory hull organic fertilizer. Aiming at the characteristics that lignin in the hull has a high content, and is difficult to break down, trametes gallic which has strong adaptability and is capable of breaking down lignin and cellulose is added during the piled aging, paint enzymes and lignin peroxidases generated in the growth process of the trametes gallic can promote conversion of lignin to humic acid, and simultaneously, lignin wrapped cellulose is released; and after the cellulose is treated at high temperature in the aerobic fermentation process, the cellulose is changed in structure, so that the cellulose is easily utilized by microbes, the material is composted more thoroughly, and the content of humic acid in the product is increased.

This invention relates to a epiphyte co-culture ferment Laccase production method, which is to inoculate the bacterium detergent of the Trametes AH28-2(Trametes sp. AH28-2) in the ferment culture medium under 22-37deg C for 1-4 days, then adding the bacterium detergent of Trichoderma ZH1(Trichoderma sp. ZH1) to culture for 5-10 days, finally separating to obtain the Laccase preparation, wherein the enzyme activity is up to 6, 000U/L(guaiacol method). The culture medium is the liquid culture medium, which mainly contains a certain quantity of carbon, azotes, phosphate of kalium and natrium, sulfate of copper and ferrum, adenine and vitamin B and so on. This invention doesn't' use the aromatic compound and heavy metal iron induce, which is safe and clean, and the enzyme activity is high and stable. '

The invention relates to an artificial cultivation method and the usage of trametes lactinea berk, which respectively carries out the culture of the trametes lactinea berk by a solid culture medium and a liquid culture medium via the purification of a bacterial strain, so as to obtain an artificially cultivated trametes lactinea berk. The method can realize the large-scale artificial cultivation of the trametes lactinea berk; the drug which takes the trametes lactinea berk or the extract thereof as the main ingredient has the better functions of improving immunity and anti-tumor function, thetrametes lactinea berk obtained by the invention can be used as an additive to be added into the feed to promote the growth of the livestock and poultry, strengthen immunity and reduce diseases.

An expression system for producing a protein in a filamentous fungus, consisting of a) a host organism selected from the species Trametes and Polyporus and b) a DNS vector containing a selection marker gene. This selection marker gene code is a protein which allows the selection of positive transformants after the transformation of the host organism and which is selected from the group of: antibiotics-resistant genes coding for proteins which eliminate the growth-inhibitory effect of antibiotics against which the host organism is not resistant. Genes coding proteins which are capable of a color-causing reaction; and genes complementing a genetic defect of the host organism (auxotrophy), the expression of the selection marker gene is controlled by at least one active genetic regulation element in the host organism, and c) a DNS vector containing a gene which codes the protein to be produced. The expression of this gene and optionally, the secretion of the protein so produced are controlled by an active genetic regulation element in the host organism. The DNS vector containing a section marker gene and the DNS vector containing the gene which codes the protein to be produced can also be in the form of one DNS vector.

The invention discloses a bacterial strain for producing laccase, a method for producing laccase by the bacterial strain, the produced laccase and application of the laccase, and relates to the field of microbial enzyme. The bacterial strain for producing laccase is trametes pubescens (Trametes pubescens BJFC-C1108) with a microbial preservation number of CGMCC No. 10308. By utilizing the trametes pubescens (Trametes pubescens BJFC-C1108), the yield of the produced laccase is relatively high; the enzyme activity can be up to 28.687 U/mL; the prepared laccase has relatively high activity, stability and tolerance under the alkali condition (pH 7.0-10.0), has relatively high tolerance for metal ions, can be used for shortening the time required by complete decoloring of azo dye Congo red to a certain extent, has a potential application value, can be applied to more industrial fields and has favorable development and application prospects.

The invention discloses trametes hirsute SEM-6 (trametes trogii SEM-6). The strain is preserved at the China Center for Type Culture Collection on January 6, 2014, and the preservation number is CCTCC N0.M2014002. Application of the trametes hirsute strain in production of laccase by using municipal dewatered sludge belongs to the technical field of bioengineering. The municipal dewatered sludge of which the organic matter content is 40-60% (according to dry weight) is taken as a main fermentation substrate to produce laccase in a liquid fermentation or solid fermentation manner. Thus, high-value utilization of an organic component in the sludge is achieved. The produced laccase can be applied to the aspects such as lignocellulose pretreatment, environmental pollutant treatment and the like.

The invention relates to a method for preparing a protein feed by mixing straw and straw dilute acid hydrolytic pentose butanol or alcoholic fermentation waste water for solid state fermentation, which comprises the following steps of: mixing the steam-exploded straw or the straw hydrolyzed by dilute acid and the straw dilute acid hydrolytic pentose butanol or alcoholic fermentation waste water in a certain volume to ensure that moisture content is between 70 and 80 percent, after sterilizing, inoculating penicillium decumbens and trametes pubescens to the mixture and performing aerobic solidstate fermentation for 4 to 6 days, inoculating candida tropicalis and lactobacillus plantarum and performing anaerobic fermentation for 2 to 3 days, and drying to obtain the protein feed. In the protein feed product prepared by the method, the protein content is between 22 and 27 percent, and the degradation rate of coarse fibers is between 63 and 68 percent. The method can solve the problem of discharging the butanol or alcoholic fermentation waste water; and the waste water and the straw are converted into the protein feed, so that the waste is turned into treasure.

The invention discloses a traditional Chinese medicine combination for treating porcine pseudorabies and a preparation method and application thereof. The combination is prepared with 15 to 30 parts by weight of sheepear inula herb, 6 to 18 parts by weight of trametes robiniophila murr and 5 to 15 parts by weight of fiveleaf gynostemma herb. The preparation method for the combination includes thefollowing steps: the sheepear inula herb, the trametes robiniophila murr and the fiveleaf gynostemma herb are decocted for 2 to 3 times; the decoctions are combined, concentrated to be thick and mixed with ethanol until the ethanol concentration is 60 to 70 percent; after deposition and filtration, the concentrated liquor is then mixed with ethanol until the ethanol concentration is 75 to 80 percent; after deposition, filtration and filtrate concentration, the liquor is added with distilled water and refrigerated for deposition; and after filtration and concentration, the traditional Chinese medicine combination is obtained. When being used for treating porcine pseudorabies, the traditional Chinese medicine combination has little side effects and a high curative rate, and is safe and convenient to use.

The invention belongs to the technical field of wine making and provides a kiwifruit wine making method. The method includes steps of kiwifruit selection, kiwifruit pretreatment, fermentation, ageing and clarifying, wherein kiwifruit pretreatment includes color protection and enzymolysis. A homemade yeast for fermentation is prepared from, by weight, 300-400 parts of glutinous rice, 100-120 parts of piper wallichii, 60-80 parts of whole pueraria powder, 150-200 parts of starfruit, 50-60 parts of trametes orentalis, 20-26 parts of pleurotus eryngii, 20-30 parts of flos sesamum indicum and 20-30 parts of flos mume. By adoption of the yeast prepared from Chinese herbal medicines for fermentation of kiwifruits, growth and metabolism of saccharomycetes in a fermentation process can be promoted while growth of harmful bacteria is inhibited, and accordingly qualities of aroma, color, taste and the like of kiwifruit wine can be improved, and better healthcare efficacies of the kiwifruit wine can be achieved.

The invention discloses a banana stem and leaf residue decomposing inoculant for inhibiting fusarium wilt and a preparation method thereof. The banana stem and leaf residue decomposing inoculant is prepared by using trametes versicolor with a cellulose, lignin and hemicellulose decomposing function, trichoderma citrinoviride, aspergillus oryzae, bacillus amyloliquefaciens with a biological control function, and streptomyces pristinaespiralis for compound fermentation. The banana stem and leaf residue decomposing inoculant can effectively accelerate the decaying of banana stem and leaf residues returned to the field, while inhibiting the growth of fusarium wilt of banana and other pathogenic bacteria; the decomposition of the banana stem and leaf residues is accelerated after applying the decomposing inoculant in the field, wherein the weight loss rate of the banana stem and leaf residues is increased remarkably; the tensile force of the banana stem and leaf residues is reduced remarkably; the number of soil pathogens including antagonistic bacillus and actinomycetes is increased remarkably; and the number of pathogenic organisms is reduced remarkably.

The invention discloses a new strain, namely Trametes versicolor T-4 capable of efficiently preprocessing lignin raw materials under the alkaline condition. The Trametes versicolor T-4 is preserved in the CGMCC (China General Microbiological Culture Collection Center) at Sep.2nd in 2014 with the preservation number of CGMCC No.9582, and the address of the CGMCC is Microbiology Research Institute of China Science Academy, No.3 of No.1 yard at Beichen west road of Chaoyang District in Beijing. The Trametes versicolor T-4, provided by the invention, can solve a series of problems that the biological preprocessing of the lignin raw materials is low in efficiency and long in cycle and the like, and has great potential in the aspects such as preparation of fuel ethanol and methane through the utilization of the lignin raw materials, and lays the foundation for the clean, efficient and low-cost preprocessing of the lignin raw materials.

The present invention provides trametes robiniophila polysaccharide protein, a preparation method therefor, and an application thereof. Monosaccharide compositions of the trametes robiniophila polysaccharide protein of the present invention are fucose, arabinose, galactose, glucose, xylose, and mannose, a mass ratio thereof is 3.2:2.0:33.5:5.5:1.2:50.4, and the weight-average molecular weight of the polysaccharide protein is 7.0x105-5.0x106Da and preferably is 2.69x106Da. The polysaccharide protein of the present invention can be used to prepare medicine for curing tumour.

The invention relates to the field of crop cultivation, in particular to a trametes robinioplila cultivating method. The method comprises the steps that a primary stock culture of trametes robinioplila is inoculated in a first culture medium for the first time of spawn running to obtain a secondary stock culture, the secondary stock culture is inoculated on a rearranged first culture medium for the second time of spawn running to obtain a tertiary cultivated cultivar, and the tertiary cultivated cultivar is inoculated on a second culture medium for the third time of spawn running to obtain the trametes robinioplila. Compared with the trametes robinioplila obtaining method common in the prior art that the trametes robinioplila is obtained by picking directly from a host tree species such as a Chinese scholar tree, a locust and a Pteroceltis tatarinowii Maxim, the trametes robinioplila cultivating method has the advantage that artificial cultivation of the trametes robinioplila is achieved. A large amount of trametes robinioplila can be obtained through mass artificial cultivation, so that huge demand of the medicinal material market for the trametes robinioplila is met.

The invention belongs to the field of applied microbiology and the technical field of bioengineering, and particularly discloses trametes trogii laccase isozyme TtLac13. The amino acid sequence of the trametes trogii laccase isozyme TtLac13 is shown in SEQ ID NO:1. When the trametes trogii laccase isozyme TtLac13 is applied to biodegradation of triphenylmethane colored and toxic substances such as peacock green and crystal violet, time is short, decoloration efficiency is high, good industrial application prospects are achieved, and good economic benefits and good social benefits can be generated.

The invention discloses a high-yield laccase fungus named as trametes hirsute La-7 which is collected at the China General Microbiological Culture Collection Center at 3# of Yard 1, Beichenxi Road, Chaoyang District, Beijing on Sept. 27th, 2019 under the number of CGMCC No 5.2199. Extracellular crude laccase produced by fermenting trametes hirsute La-7 can effectively remove 17 beta-estradiol in natural water and livestock-poultry manure.

The invention relates to a trametes hirsute strain, application of the trametes hirsute strain and a method for processing Pu'er tea. The trametes hirsute strain has the preservation number of CGMCC No.8752. The strain can be used for processing Pu'er tea. The method for processing Pu'er tea comprises the following steps of inoculating separated and purified trametes hirsute thalli into big leaf crude tea, and performing fermentation culture. The trametes hirsute strain has the advantages that the fermentation quality of the Pu'er tea can be easily controlled under the artificial fermentation condition; the fermentation quality is stable; the fermented Pu'er tea with the improved sensory quality can be obtained. In addition, the conversion of substances contained in the Pu'er tea can be effectively promoted; the pharmacological and health care functions of the Pu'er tea can be increased; and the fermentation time can be shortened.

The invention discloses a method for quick harmless recognition of adulterated samples of a trametes versicolor extract based on PLS-DA. The method comprises the following steps of (1) collecting a near infrared reflectance spectrum of trametes versicolor extract samples and adulterated trametes versicolor extract samples, wherein the spectrum wavelength range is 12400-4000cm<-1>, the resolution is 8cm<-1>, and the number of scanning times is 16, and taking average spectra as the original spectra of the samples; (2) adopting a first derivative, in combination with polanary scattering calibration, and performing pretreatment on the original spectral information obtained in step (1), so as to remove influence of spectra noise, baseline wander and the like; (3) determining the main component factor number to be 2, setting the classification variable of the trametes versicolor extract to be 1 and the classifying variable of the adulterated samples to be 0, and establishing a partial least square judgment model; and (4) bringing unknown trametes versicolor extract samples into the model in the step (3), if the classification variable value of the unknown trametes versicolor extract samples is between 0.54-1.28, determining that the unknown trametes versicolor extract sample is the trametes versicolor extract, else, determining that the unknown trametes versicolor extract sample is the adulterated trametes versicolor extract.

The invention relates to a method for extracting pepstatin through fermenting trametes. The method comprises the following steps: freezing and drying a proper amount of trametes to a constant weight, grinding and sieving; placing sieved sample powder in an inclined plane culture medium to carry out culturing; placing activated strains into a fermentation flask to carry out flask shaking fermentation culture so as to obtain strain fermentation liquor; and finally, carrying out ultrasonic cell breakage and centrifuging on the strain fermentation liquor, carrying out decoloration through macroporous resin, taking supernate to carry out dialysis and then carrying out purification on the pepstatin so as to obtain the pepstatin. According to the method, the ultrasonic extracting conditions are strictly limited, so that the maximum release of the pepstatin is ensured, the pepstatin is also prevented from being damaged through ultrasonic extraction for excessively long time at an excessively high temperature, and the extracted pepstatin has a good inhibiting effect.

The invention discloses an application of a composite in preparing medicine for treating nephropathy. The composite contains water extractive of raw medicinal materials. The raw medicinal materials comprise trametes robinioplila murr, wolfberry and sealwort.

The invention relates to a biological feed which is prepared by compounding a saussurea residue complex probiotic fermented product and a saussurea residue trametes robiniophila fermented product. Thebiological feed contains rich active ingredients including amino acid, vitamins, polysaccharides, alkaloid and the like, and has a remarkable effect of reinforcing immunity due to the comprehensive effect of the components.