365results about How to "Strong growth inhibitory effect" patented technology

The present invention relates to a therapeutic method using RNAi directed at BRAF, of which the point mutation, especially V599E, occurs frequently in melanomas. RNAi specific for the mutated BRAF will provide a specific therapeutic intervention for cancers such as malignant melanoma. Several target sequences for RNAi were selected in the protein coding region of the BRAF mRNA. The short hairpin RNA expression cassette was constructed on the lentiviral vector. One recombinant viral vector for the mutated BRAF V599E and two other vectors sites for wild type BRAF were constructed to infect various malignant melanoma cell lines, and the effects on the growth inhibition and the signaling of MAPK pathway were examined. The inhibitory effect on the invasion ability of malignant melanoma cell line and in vivo growth of a malignant melanoma cell line were examined.

The invention relates to a method for preparing a biologic herbicide-containing fertilizer from biogas slurry by using biogas slurry as a main raw material. The method is characterized by comprising the following steps of: screening biogas slurry with a sieve of 120 meshes, and adding a compound microorganism bactericide prepared from antagonistic bacilli to prepare the biologic herbicide-containing fertilizer. In the biologic herbicide-containing fertilizer, the number of antagonistic bacilli reaches 5-6*10<7> CFU / ml. The antagonistic bacilli comprise geobacilli, bacillus amyloliquefaciens and bacillus subtilis.

The invention discloses a bacillus amyloliquefacien and a preparation method and application thereof. According to the bacillus amyloliquefacien and the preparation method and the application thereof, the culture collection number of the bacillus amyloliquefaciens H6 is CCTCC No: M2013249; the bacillus amyloliquefaciens has obvious growth inhibition effect for gram-positive bacteria and gram-negative bacteria, strong degradation ability for starch, albumen, and cellulose, strong environment tolerance, and excellent effect for preventing diarrhea and promoting growing, and can not produce adverse effect with large doses of feeding; and the rear condition is simple, the cost is low, and the development and application prospect is excellent.

The invention discloses a spray for killing mites on the body surface of a pet and a preparation method of the spray. The spray is simple in preparation method, convenient to use, good in curative effect and high in safety. The spray for killing the mites on the body surface of the pet is technically characterized by comprising the following ingredients in parts by weight: 75-95 parts of alcohol, 0.1-1 part of flucycloxuron, 0.1-1 part of phoxim, 5-20 parts of traditional Chinese medicine combination extracts, and 0.1-1 part of eugenol, wherein a traditional Chinese medicine composition comprises the following raw materials in parts by weight: 15-20 parts of radix stemonae, 3-5 parts of fructus cnidii, 3-5 parts of sophora flavescens, 2-4 parts of cortex phellodendri, 3-5 parts of cortex dictamni, and 4-7 parts of fructus kochiae. The spray belongs to the technical field of animal medicines.

The invention discloses a pro-drug as shown in general formula (I), which is formed by coupling camptothecin or a derivative of the camptothecin with non-linear configuration glycol polyethylene, wherein, the definition for varied groups is available in the specification. A physiological disposition research on nude mice discloses that: the pro-drug has a time-delay plasma concentration - time curve, and the plasma concentration can still be maintained at over 100ng / ml 72 hours after intravenous medication. Evaluation on physiological drug effect shows that the pro-drug has strong growth inhibiting effect against the LOVO HCT116 cell line transplantable tumor inoculated into the nude mice and also has obvious growth inhibitory activity against human lung carcinoma H460 cell line transportable tumor inoculated into the nude mice. The inhibition of the pro-drug is better than that of Irinotecan, a positive control drug and has no obvious systemic toxicity.

The invention discloses a modified plant fiber material type glass moldproof parting powder which is prepared by carrying out surface modification on plant fiber pulverized substances. The preparation method comprises the following steps: screening plant fiber pulverized substances, mixing with a modifier solution, standing, treating under the absolute pressure of 100-500kPa at the temperature of 0-300 DEG C for 0.1-48 hours, and finally, directly drying to obtain the modified plant fiber material type glass moldproof parting powder. The modifier is selected from one or mixture of organic acids, inorganic acids or acid salts. The moldproof parting powder has the advantages of favorable flowability, adsorptivity and natural degradability, can postpone the glass from becoming moldy, basically does not generate flying ash, and is clean and convenient. The glass using the product can be easily cleaned after long-term storage, and can not influence the subsequent processing and use of the glass.

The invention discloses an oleanolic acid oxime ester derivate compound, a preparation method and an application thereof. The structural formula of the compound is shown in formula I, and in the formula I, R refers to furyl, pyridyl, 1-menaphthyl, 4-chlorophenoxy methyl or phenyl with 1 to 2 substitutional groups; and the substitutional groups in phenyl with 1 to 2 substitutional groups are halogen (such as fluorine, chlorine, bromine and iodine) or nitryl. The preparation method comprises the following steps: 1) performing the esterification reaction between oleanolic oxime subject to benzylation protection and substituted carboxylic acids to prepare an oxime ester compound; and 2) removing benzylation protection on the oxime ester compound by Pd / C-H2 to prepare the compound in the formula I. The bactericidal activity test result shows that CAU2012-A has a favorable effect of inhibiting growth of sclerotinia scleotiorum, phytophthora melongenae, botrytis cintrea, pyricularia grisea, rhizoctonia solani and cotton fusarium wilt.

The invention provides a preparation method of sargassum fusiforme selenium polysaccharide, and relates to a preparation method of polysaccharide, aiming at solving the technical problems that the existing selenium polysaccharide synthesizing method is complex to operate, the synthesized selenium polysaccharide is pungent in smell, and the used reagent is toxic. The method comprises the following steps of: 1, adding 1g of sargassum fusiforme polysaccharide into 100mL of 0.5% by volume HNO3 water solution to obtain a polysaccharide solution; 2, adding sodium selenite into the polysaccharide solution to obtain a mixed solution, wherein the mass ratio of sodium selenite to the sargassum fusiforme polysaccharide in the mixed solution is (0.4-1.2g):1g, reacting for 6-10hours at the temperature of 50DEG C-90DEG C, cooling, adding NaCO3 to adjust the pH value to 5-6, centrifuging, dialyzing till the red is not detected to be unavailable after ascorbic acid is added into dialyzate, heating and concentrating, and carrying out freeze drying to obtain the sargassum fusiforme selenium polysaccharide. The preparation method provided by the invention is simple to operate, and free of pungent smell, and the used reagent is non-toxic and environment-friendly.

Production of anti-insect and disease-proof mold and its use are disclosed. The process includes taking hamycin, Agrobacterium tumefaciens AGL-1 and gene plasmid pPBH with thuricin bacillosporin stored in normal way, preparing conidiospore suspension, converting the obtained tumefaciens and pPBH plasmid, activation culturing, culturing the activated fungus liquid of conidiospore suspension, tumefaciens and pPBH plasmid mutually, converting, screening and purifying. It can be used to prepare anti-insect and disease-proof mold and insect prevention of plant.

The invention relates to the technical field of microbes. The eucommia ulmoides endogenous bacteria are separated from eucommia ulmoides living plants by adopting an endogenous bacteria microbe separation technology. Through identification by microbial taxonomy, strain is named Bacillus pumilus DZBT01 and is collected in China General Microbiological Culture Collection Center, the collection number is January 30, 2015, and the collection register number is CGMCC NO.10470. The strain disclosed by the invention has the characteristics that the strain can achieve an obvious effect of inhibiting plant pathogenic fungi. The invention discloses an application of the Bacillus pumilus DZBT01 in control of wheat sharp eyespot and an application of Bacillus pumilus in promotion of wheat growth. The Bacillus pumilus DZBT01 disclosed by the invention refers to eucommia ulmoides plant endogenous bacteria, is simple in culture conditions and easy to preserve and refers to an agriculturally important microbial resource.

The invention relates to a medicine with effective component of plant nodicorn, which is characterized in that: said effective components can be polyphenol and monomer caffeic acid 3, 4- dihydroxyphenylglycol; the monomer caffeic acid 3, 4- dihydroxyphenylglycol is extracted and purified from nodicorn and other plants; the preparation of polyphenol and the extraction of monomer caffeic acid 3, 4- dihydroxyphenylglycol comprise: refluxing and extracting alcohols in different densities, boiling, extracting butanol, macroreticular resinous adsorbent chromatography and positive and passive silicon gel chromatography. The invention can treat cancer, attenuate, and treat general bacteria infection.

The invention provides a pyrimidine compound, and the structure of the pyrimidine compound is shown in the following formulas (I) and (II), wherein R is substituted phenyl or substituted pyridyl; R1 is -NO2, -Br, -COOH or -OCH3; n is an integer of 1-3; X is -Br or CI; and R8, R9 and R10 are identical or different -H, -OCH3, -COOMe, -Br, -COOEt, -CH2COOMe, -NO2 or (CH2)1-4OH. The pyrimidine compound provided by the invention has an effect of FAK inhibition, can effectively enter tumor cells, has a good effect of inhibiting the tumor cells and can be better detained. The invention also provides a preparation method of the compound and the application of the compound to the preparation of preparing a tumor inhibitor.

The invention discloses an anthraquinone compound having the formula shown in the following formula (I) or formula (II). The anthraquinone compound with a novel structure is a natural product obtainedby fermenting and purifying rice by actinomycetes, has obvious anti-tumor activity, especially has significant proliferation inhibition for human prostate cancer cell line PC3 cells and human colon cancer cell line SW620 cells, and can be used for developing anti-tumor drugs. (The formulas are shown in the description.).

treating method for hair growth inhibition, which comprises administering (A) the extract of a plant of the family Juniperus or a malt. In addition, the present invention relates to a dermatologic composition for external use, which comprises (B) an elastase inhibitor or neutral endopeptidase inhibitor, and the above-described component (A) and / or (C) at least one proteolytic enzyme selected from the group consisting of papain, trypsin, chymotrypsin, pepsin, bromelain, ficin and pancreatin.

The invention discloses a resveratrol benzene acrylamide derivative, a preparing method and an application thereof. The resveratrol benzene acrylamide derivative has a structure shown in general formula (1) and general formula (2): the resveratrol benzene acrylamide derivative has an obvious effect of growth inhibition for a human body breast cancer cell line (MCF-7), a human body lung cancer cell line (A549) and a human body melanoma cell line (B16-F10), thereby the resveratrol benzene acrylamide derivative can be used for preparing antineoplastic drugs.

The invention belongs to the field of pharmacy and tumor diagnosis and treatment, and particularly relates to a compound serving as a cyclin-dependent kinase 7 (CDK7) inhibitor, and application of thecompound in treatment of proliferative diseases such as cancers including breast cancer, ovarian cancer, colon cancer, liver cancer, lung cancer and glioma. The CDK7 small-molecule inhibitor is a compound with the structural general formulas (I)-(X), or a stereoisomer, a pharmaceutically acceptable salt, solvate, hydrate, polymorph, co-crystal, tautomer, isotope labeled derivative or prodrug thereof. The small-molecule inhibitor provided by the invention is wide in anticancer spectrum; the compound has a good growth inhibition effect on various cancer cells such as liver cancer, stomach cancer, colorectal cancer, ovarian cancer, glioma, lung cancer and the like, provides an effective drug for preventing or treating proliferative diseases, especially cancers, in the aspects of structure and action mechanism, and has a good application prospect.

The invention discloses Taiwania flousiana Gaussen with herbicidal activity and an application of Taiwania flousiana Gaussen and an application of a Taiwania flousiana Gaussen extract as a herbicide. The research finds that the Taiwania flousiana Gaussen and the Taiwania flousiana Gaussen extract have the efficacies of remarkably inhibiting germination, synthesis of chlorophyll a and growth of multiple classical farmland weeds, invasive weeds and aquatic weeds such as crab grass, amaranthus retroflexus, tropic ageratum herb, feather cockscomb, Bidens pilosa, mikania micrantha, Pistia stratiotes and spirogyra, have remarkable inhibition effects, can be applied to the prevention and kill-off of the farmland weeds and can be developed into efficient and pollution-free environment-friendly herbicides, or a lead compound is provided for the creation of novel herbicides; and the Taiwania flousiana Gaussen and the Taiwania flousiana Gaussen extract have remarkable social and economic benefits and good application prospects.

The invention discloses cell oncosis induction cyclometallated iridium complexes, and a preparation method and an antitumor application thereof. The structural formula of the cyclometallated iridium complexes is shown in the description. The cyclometallated iridium complexes have very strong growth inhibition ability to various human cancer cells comprising drug-resistant strains, and the action mechanism is based on a new mechanism for inducing cell oncosis not apoptosis based on mitochondrial targeting not traditional nuclear targeting. The complexes overcome the defect of the drug resistance of tumor cells during tumor therapy, and have very good application prospect in the development of high-efficiency antitumor drugs.

The invention relates to an anti-tumor pharmaceutical composition. The pharmaceutical composition is characterized by comprising ursolic acid and cyclophosphamide with a weight ratio of (1-10):1, and the preferable ratio is (2-5):1. The ursolic acid and the cyclophosphamide are cooperatively used for treating tumors to generate an obvious synergistic effect. The composition can both reinforce the anti-tumor efficacy of a single component and reduce the toxicity on normal tissues, so as to improve the tumor treatment effect, and thus the pharmaceutical composition has a broad application prospect in the field of tumor treatment.

The invention relates to an application for extracting natural compound (I) from black mulberry, the compound (I) has strong inhibition effect for tyrosinase and is applied for preventing skin mottle, freckle, senile plaque or the like skin chromatosis and whitening, and the compound (I) is a food additive. The activity of the compound (I) is strong proved by the mensuration of the inhibition activity, the compound (I) has good stability and obvious growth inhibition action for the melanoma cell, can reduce the activity of the tyrosinase in the melanoma cell and biological synthesis level of the melanin in the cell.

The invention provides an ursolic acid derivative, which is N-[3beta-acetoxy-ursa-12- alkene-28-acyl]-aminoethylenediamine-folic acid. A natural product ursolic acid is structurally transformed to obtain N-[3beta-acetoxy-ursa-12- alkene-28-acyl]-aminoethylenediamine-folic acid (I), N-[3beta-acetoxy-ursa-12-alkene-28-acyl]-aminoethylenediamine (III) and N-[3beta-acetoxy-ursa-12- alkene-28-acyl]-aminoethylenediamine (IV). The in vitro cell proliferation inhibition test shows that the proliferation inhibition effect of the compounds I, III and IV on tested tumor cell lines HepG2, of BGC823, A-375, Hela and the like is superior to that of the ursolic acid, and the toxic effect of the compounds I, III and IV on human embryonic lung fibroblasts (HELF) is lower than that of the ursolic acid. The in vitro anti-tumor effect of the compound III is the best, and the compound I has certain targeting.

The invention discloses a copper complex and the preparation method and application thereof, providing a cupric complex with 1, 10-phenanthroline, nitrate radical and N, N-dimethylfomamide. The preparation method of the cupric complex with 1, 10-phenanthroline is that nantokite, 1, 10-phenanthroline, nitrate radical and N, N-dimethylfomamide are mixed to react in solution to obtain the complex. The synthetic materials of the complex of the invention are easy to obtain and have low cost; the product precipitates in the form of crystalloid, thereby having a high purity and high production efficiency; besides, the complex can exist stably in natural states and has good water-solubility and fat-solubility; in addition, the complex of the invention has a good inhibiting effect on a plurality of cancer cells of lung cancer, liver cancer, adenocarcinoma of colon and so on, extensive effect and wide application range.

The invention belongs to the battery material field, in particular to a nanometer Zn @ C negative electrode material for a secondary zinc-based battery and a preparation method thereof. C in the negative electrode material is a nano carbon material, the thickness of the negative electrode material is 50-100 [mu]m and a Zn grain size is 20-80 nm; the preparation method comprises the following steps: adding nano-carbon material into nano-zinc plating solution for electroplating, then washing with water and drying to obtain the nano-Zn @ C negative electrode material; DC, high frequency forward pulse current or high frequency bi-directional pulse current can be used for electroplating; The nanometer zinc plating solution comprises zinc sulfate, boric acid and grain refiner, the pH value is 1to 3, and the solvent is water. The negative electrode material prepared by the invention has good adhesion, no zinc shedding after 20 times of 90 DEG bending tests, strong dendritic growth inhibitionability, excellent specific capacity, cycle life, coulomb efficiency and magnification performance, and is proved to be good in effect by magnification test and suitable for large-scale industrial production.

The invention relates to flavones separated and purified from shepherd's purse. The method comprises the following steps: ethanol ultrasonic extraction, solvent extraction, silica gel column chromatography eluting separation, resin column adsorption, silica gel column chromatography eluting separation, vacuum concentration, vacuum drying and the like. The name of the separated flavones is 2-(3,4-dimethoxyphenyl)-5-(hydroxymethyl-7-O-beta-D-glucosyl-4H-chromene-4-one. The raw material, which is the medicinal and edible shepherd's purse, has abundant sources and is cheap and accessible. The prepared 5-hydroxymethyl new flavones have novel structure, have obvious effect of inhibiting tumor cell proliferation, and can be used as a cell proliferation inhibitor or antitumor agent for antitumor research. The flavones have favorable development and utilization values, and lay foundation for research and development of clinical drugs for preventing and treating tumors in future.

The invention provides a pharmaceutical composition and a preparation method thereof. The pharmaceutical composition comprises a carrier and an active ingredient loaded on the carrier, wherein the carrier is a nanoparticle of a hyaluronic acid-cystamine-polylactic acid-glycolic acid grafted polymer as shown in a formula (I) as shown in the specification; in the formula (I), n is an integer ranging from 16 to 30, the ratio of Y to X ranges from (1-3): 1, Y is an integer ranging from 153 to 549 and X is an integer ranging from 73-385. The pharmaceutical composition is capable of realizing directional delivery so that the medicine can be released quickly in cells and the effect of the medicine can be improved.

The invention relates to Cu-Eu exotic-metal-substituted arsenotungstate which has a chemical formula as follows: Na2K3.5Eu0.5[Eu(H2O)7][As2W19Cu2O67(H2O)3].22H2O. A compound is prepared by reacting EuCl3, CuCl2.2H2O, NaCl and an arsenotungstate precursor-K14[As2W19O67(H2O)] in an aqueous solution. A preparation method is simple, easy to operate and relatively low in cost. Tests discover that the compound has a remarkable inhibition effect on the growth of human hepatocarcinoma cells HepG2 and human colonic cancer cells HCT-116 and can be used for preparing drugs for resisting cancers, especially liver cancers and colonic cancers, and research results provide an experimental basis for application of the compound to an aspect of drug activity.

An application of Chinese cephalotaxus in preparing herbicide includes extracting the herbiciding component in organic solvent, separating and preparing herbicide. Its advantage is high herbiciding activity to weeds including wild oats, barnyard grass, etc.

The invention discloses a production method for sophorolipid with a hair growing effect. According to the production method, reprocessed oils extracted from animals or plants serve as raw materials, and a hydrolysis method of the raw material oils, a fermentation method of sophorolipid and an extraction and separation method are included. The sophorolipid has better hair growing and hair losing resisting effects when applied to cosmetic production, and is natural and nontoxic, capable of promoting cell proliferation and enhancing cell metabolism and beneficial to hair growth.

The invention provides aminoquinazolinone and aminoisoquinolinone derivatives. Through multiple experiments, it is validated that the synthesized compounds have excellent PI3Kalpha and PI3Kgamma inhibition effects, and most of the compounds have a significant inhibition effect on PI3Kalpha, and show a potent growth inhibition effect on tumor cell lines such as a PIK3CA-mutated human breast cancercell line (MCF7). Therefore, the aminoquinazolinone and aminoisoquinolinone derivatives can be applied to preparing antitumor and anti-inflammatory drugs, and can be applied to drugs for the treatmentof human or animal cell proliferation-related tumors as a PI3Kalpha inhibitor. The drugs are prepared from any one or more of the derivatives, and pharmaceutically acceptable salt and solvate of thederivatives, and a pharmaceutically acceptable carrier. The general formulas a and b of the compounds are shown in the description.

The invention relates to an alpha, beta-unsaturated ketone compound containing a 1,2,4-oxadiazoles heterocycle, the structure of which is as shown in general formula 5, wherein R1 is hydrogen, methyl or chlorine; R2 is methyl, chlorine or bromine; R3 is methyl or ethyl; R4 is para-trifluoromethyl, ortho-methyl or para-nitro; and R5 is hydrogen or methyl. The invention also provides a preparation method of the compound and application thereof. The half-growth inhibitory concentration of the compound on HL-60 cells is less than or equal to 2.0 mu M, and the activity is obviously improved, thus the compound can be used for preparing antileukemie medicaments.