49 results about "Microbial Taxonomy" patented technology

The invention discloses an endophytic fungus in salvia miltiorrhiza bunge. The endophytic fungus is isolated and obtained from the living body of salvia miltiorrhiza bunge by adopting the endophytic fungus isolation and purification technology and is identified to be trichoderma atroviride through microbial taxonomy. The strain was collected in the China General Microbiological Culture Collection Center, with collection number being CGMCC No.4712.. Tanshinone compounds tanshinone I and tanshinone IIA are generated through liquid fermentation of the strain of the endophytic fungus in salvia miltiorrhiza bunge, so the endophytic fungus is an important microorganism for finding the new resources such as the tanshinone chemical components tanshinone I and tanshinone IIA.

The invention discloses a huperzia serrata endophytic fungus for producing huperzine A, and belongs to the technical field of biological engineering. The fungus is obtained by separating from fern H. serrata living plant according to the endophytic fungus separation and purification technology, named as shiraia sp. slf-14 by microbial taxonomy authentication and collected in CCTCC on Nov. 6th, 2009 with the collection number of CCTCC No: M 209294. The strain can produce the huperzine A by liquid fermentation. The invention discovers the huperzine A contained in the shiraia sp. fermentation liquid for the first time, and provides a new path for seeking an active huperzine A component resource for treating senile dementia.

The invention relates to the field of biotechnology, in particular to application of alternaria alternate metabolic products in cucumber rhizoctonia solani prevention and control. In the invention, endophytic fungus of camphor trees is separated from camphor tree living bodies collected from Tianmu mountain in Hangzhou, Zhejiang province by adopting endophytic fungus separation technology, and isnamed alternaria alternata 31 through microbial taxonomy identification, and the strain is stored in China General Microbiological Culture Collector Center (CGMCCC) on December 31, 2010, and the collection register number is CGMCC No.4508. The invention has a most obvious characteristic that the strain liquid can be fermented to produce active metabolic products which have inhibiting effect on pathogenic bacteria of plant fungus diseases such as Rhizoctonia solani, Fusarium oxysporium, Botrytis cinerea and the like, thus alternaria alternate is an important microbial resource in agriculture.

The invention discloses bacillus amyloliquefaciens separated from fermented soya beans and used for producing a protease and belongs to the technical field of biological engineering. The bacillus amyloliquefaciens is separated from traditional fermented soya beans by adopting a separation and purification technique. The bacillus amyloliquefaciens which is identified and named according to the microbial taxonomy is collected in the China Center for Type Culture Collection (CCTCC) on December 10. 2014 with the collection number of CCTCC M 2014639. The bacillus amyloliquefaciens used for producing protease is easy to culture and is highly safe during production, the screened strain for liquid fermenting and culturing has the enzyme activity of 148-2,231U / mL, has good genetic stability; the metabolite is easy to separate and purify; a foundation is laid for the scientific pure culture in the traditional fermented soya bean industry.

The invention relates to microbe technique field, endophytic fungus of Chinese Holly of the invention collected from Chinese Holly living plant of Tianmu Mountain of Linan City is obtained by endophytic fungus microbe separation technology, named as Trichoderma harzianum Rifai stem L identified by microbial taxonomy stored by general microbilogy centre of China Forestry Culture Collection Center on date of august 14th 2006, registration number is CGMCC NO.1780. The most prominent feature is the stem can generate activated component seaquiterpenoids Trichothec-9-en-4-ol,12,13-epoxy-,acetate,(4beta)-(8CI,9CI) by fermentation for inhibiting phytopathogenic fungi bacteria, it is an important microbe resource in agriculture.

The invention relates to the technical field of microbes. The eucommia ulmoides endogenous bacteria are separated from eucommia ulmoides living plants by adopting an endogenous bacteria microbe separation technology. Through identification by microbial taxonomy, strain is named Bacillus pumilus DZBT01 and is collected in China General Microbiological Culture Collection Center, the collection number is January 30, 2015, and the collection register number is CGMCC NO.10470. The strain disclosed by the invention has the characteristics that the strain can achieve an obvious effect of inhibiting plant pathogenic fungi. The invention discloses an application of the Bacillus pumilus DZBT01 in control of wheat sharp eyespot and an application of Bacillus pumilus in promotion of wheat growth. The Bacillus pumilus DZBT01 disclosed by the invention refers to eucommia ulmoides plant endogenous bacteria, is simple in culture conditions and easy to preserve and refers to an agriculturally important microbial resource.

The invention discloses dendrobium officinale Kimura et Migo endophytic fungus. The dendrobium officinale Kimura et Migo endophytic fungus is obtained by separating from the living body of dendrobium officinale Kimura et Migo belonging to dendrobium species, orchidaceae by adopting an endophytic fungus separation and purification technology; and identified by the microbial taxonomy, the dendrobium officinale Kimura et Migo endophytic fungus is Pestalotiopsis sp.DO14; the strain is preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number CCTCC M 2015180. Through co-culture with dendrobium officinale Kimura et Migo tissue culture seedlings, the steam thickening and lengthening, and the stem and leaf color deepening of the dendrobium officinale Kimura et Migo tissue culture seedlings can be promoted, the improvement of the polysaccharide content in the stem can be promoted, the change of alcohol soluble ingredients in the stem and the flavonoid ingredients in the leaves can be promoted; the dendrobium officinale Kimura et Migo endophytic fungus is an important microbe closely related to the growth and metabolites of dendrobium officinale Kimura et Migo, and has the application potential of promoting the growth of dendrobium officinale Kimura et Migo and the change of the metabolic components.

The invention provides biocontrol endophytic fungi- Alternaria alternata and relates to the technical field of microorganisms. The endophytic fungi of camphor trees are separated from camphor tree living bodies collected from Tianmu Mountain in Hangzhou, Zhejiang by an endophytic fungi separation technique and are named as Alternaria alternata 31 according to microbial taxonomy identification. The biocontrol endophytic fungi-Alternaria alternata is collected in the General Microbiology Center of China Microorganism Strain Collection Management Committee, wherein the collection date is 2010.12.31 and the collection registered number is CGMCC No.4508. The biocontrol endophytic fungi-Alternaria alternata has the most remarkable characteristic that: the strain liquid is fermented to generate active metabolites which can inhibit plant fungi disease pathogenic bacteria such as rhizoctonia solani, fusarium oxysporium, botrytis cinerea and the like and is important microbial resources agriculturally.

The invention discloses an antagonistic strain separated and screened from a medicinal plant nothapodytes pittosporoides. According to microbial taxonomy identification, the strain is named as a bacillus amyloliquefaciens D1 strain, and a microbial agent capable of preventing and controlling powdery mildew of tobacco is prepared from the fermentation liquid of the strain. The remarkable feature of the invention is providing the new bacillus amyloliquefaciens D1 strain, the microbial agent prepared from the fermentation liquid realizes a good prevention and control effect on the powdery mildew of tobacco, and the strain is an important microbial resource in the plant growing industry.

The invention relates to a strain which is separated and screened from pinellia ternate rhizosphere soil in Dafang county of Guizhou province and has the antagonism. Through authentication of the microbial taxonomy, the strain is named as the brevibacillus brevis strain GZDF3, and biological fungicide capable of preventing and treating pinellia ternate block stem rotten sickness is obtained by adopting fermentation liquor of the strain. The strain is obviously characterized in that the new brevibacillus brevis strain GZDF3 is provided; in addition, the antibiological inoculant prepared through the fermentation liquor of the strain has the obvious antagonism on pathogenic bacteria and pathogenic fungus, such as fusarium oxysporum and fusarium solani, of the pinellia ternate block stem rotten sickness; and the strain is an important microbial resource on the traditional Chinese medicinal material green planting industry.

The invention discloses streptomyces strains and joint application thereof in prevention and treatment of cucumber fusarium wilt and relates to the technical field of microbes. Actinomycetes disclosed by the invention are obtained by separation from soil samples collected from rhizospheres of plants in Xixi Wetland, Zhejiang by adopting an actinomycetes separation technology, identified by microbial taxonomy and named as Streptomycesalbus 2013 and Streptomycestenebrarius M929, wherein S. albus 2013 was collected in China Center for Type Culture Collection on June 19, 2013 with the collection number of CCTCC NO: M2013269; and S. tenebrarius M929 was collected in China Center for Type Culture Collection on December 25, 2013 with the collection number of CCTCC NO: M2013710. The two strains can be fermented to produce active substances with relatively strong antagonistic effect against the cucumber fusarium wilt (Fusariumoxysporumf. sp. cucumebrium) and can be used for preventing and treating the cucumber fusarium wilt. When fermentation products of the two strains are mixed according to the ratio in percentage by weight of (3:2)-(1:1) to prepare a mixed preparation, the effect of preventing and treating pathogenic bacteria of the cucumber fusarium wilt is more significant than that when each of the two strains is used as a single preparation respectively.

The invention relates to the technical field of microbe. Dogbane endophytic fungus provided by the present invention is isolated from a living dogbane collected in Zhejiang Academy of Agricultural Sciences through an endophytic fungi separation technology. The dogbane endophytic fungus is identified through microbial taxonomy, and is named as nigrospora oryzae 46. The nigrospora oryzae 46 is preserved in the China General Microbiological Culture Collection Center (CGMCC). The preservation date is 2010 / 12 / 31, and the preservation register number is CGMCC No.4509. The nigrospora oryzae 46 is characterized in that: the nigrospora oryzae 46 can generate active metabolites through a liquid fermentation, and the active metabolites provide inhibitions for rhizoctonia solani, colletotrichum lindemuthianum and botrytis cinerea. Therefore, the nigrospora oryzae 46 is an important microbe resource in agriculture.

The invention discloses a ginseng endophytic fungus. The ginseng endophytic fungus is separated from a living plant body of Araliaceae Panax species Panax ginseng C.A. Mey. with an endophytic fungus separation and purification technology and is identified as Schizophyllum commune according to microbial taxonomy. The strain is collected in the CGMCC (China General Microbiological Culture Collection Center) (Institute of Microbiology, Chinese Academy of Sciences), and the collection number is CGMCC 11009. The invention further discloses an application of the ginseng endophytic fungus to increase of the biomass of tissue culture ginseng hairy roots of host ginseng and the content of ginsenosides in the host ginseng.

The invention relates to the technical field of microorganisms. Chinese holly endophyte disclosed by the invention is obtained by adopting an endophyte microorganism separation technology for separating a Chinese holly plant living body collected in Hangzhou of Zhejiang Province, is identified as streptomyces sioyaensis according to microbial taxonomy and is named as streptomyces sioyaensis107. The strain is preserved in the China General Microbiological Culture Collection Center with the preservation date of December 12th of 2012 and the preservation register number of CGMCC No.6984. The prominent characteristic of the strain is as follows: through fermentation, the liquid of the strain can generate active metabolite which has an inhibition effect on fungal diseases and pathogenic bacteria of plants such as Rhizoctonia solani of cucumber and hanatephorus cucumeris; and the strain is an important agricultural microorganism resource.

The invention discloses streptomycete bacterial strains and a combined application of the streptomycete bacterial strains for preventing and treating sclerotinia rot of colza and belongs to the technical field of microorganisms. The actinomycetes are separated from a 10-15cm deep soil sample collected in saline-alkali soil in suburb of Taian City, Shandong Province by using an actinomycetes separating technique. Authenticated by microbial taxonomy, the strains are named as streptomyces rimosus M527 and streptomyces aureofaciens M930, wherein S.rimosus M527 is preserved in China Center For Type Culture Collection on June 19th, 2013 with the preservation number of CCTCCNO: M2013270; S.aureofaciensM930 is preserved in the CCTCC on December 25th, 2013 with the preservation number of CCTCCNO:M2013711. The two bacterial strains can ferment to generate active substances which have relatively strong antagonism to sclerotinia sclerotiorum(Lib.)debary, and can be used for preventing and treating sclerotinia rot of colza. When the fermenting products of the two bacterial strains are mixed in a weight ratio of 4 to 1 to prepare a mixed preparation, the effect for preventing sclerotinia rot of colza is more remarkable than that of the two metabolites which are respectively used as single preparations.

The invention relates to the field of microbial technology and especially relates to a use of Streptomyces sioyaensis metabolite in prevention and control of Colletotrichum lindemuthianum. Llex cornuta endophyte separated from the llex cornuta living bodies in Hangzhou city of Zhejiang province by an endophyte microorganism separation technology is identified as Streptomyces sioyaensis by microbial taxonomy and is named as Streptomyces sioyaensis 107. The Streptomyces sioyaensis 107 is preserved in the China general microbiological culture collection center on December 12, 2012 and has a preservation number of CGMCC No. 6984. Through liquid fermentation, the Streptomyces sioyaensis 107 can produce active metabolites having effects of inhibiting plant fungal disease pathogenic bacteria such as Colletotrichum lindemuthianum and Alternaria solani and is an important microbe resource in agriculture.

The invention discloses a Coix lacroyma-jobi L.var.ma-yuen (Roman.) Stapf endophytic fungus which is obtained from a living plant body of the Poaceae coix plant Coix lacroyma-jobi L.var.ma-yuen (Roman.) Stapf through the endophytic fungus separation and purification technology, and is identified through the microbial taxonomy to belong to the Epichloe bromicola.The bacterial strain is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC NO.11410.By co-culturing a Coix lacroyma-jobi L.var.ma-yuen (Roman.) Stapf endophytic fungus strain, Coix lacroyma-jobi L.var.ma-yuen (Roman.) Stapf and Thale Cress, growth of Coix lacroyma-jobi L.var.ma-yuen (Roman.) Stapf and Thale Cress is promoted, growth of main roots is restrained, development of lateral roots is promoted, plants can more easily derive nutrients and grow, the biomass of Thale Cress is improved, and the Coix lacroyma-jobi L.var.ma-yuen (Roman.) Stapf endophytic fungus is an important microorganism for promoting growth of Coix lacroyma-jobi L.var.ma-yuen (Roman.) Stapf, Thale Cress and other gramineous plants.

The invention discloses a method for separating Hanseniaspora guilliermondii from red wine production raw materials or pit soil and purifying and identifying Hanseniaspora guilliermondii, comprising the steps of carrying out purification and separation on red wine production raw materials and pit soil, and conducting strain identification by combining WL nutrient agar medium morphology identification method and microbial taxonomy method to finally obtain a purified Hanseniaspora guilliermondii strain. According to the invention, the method disclosed herein in beneficial for using pure strain to culture red wind with unique pleasant fragrance; and the method has the characteristics of no bacterial contamination, easy preservation of the strain, rapid and simple operation, and stable product quality.

The invention relates to the field of microbial technology and especially relates to a use of Streptomyces sioyaensis metabolite in prevention and control of Alternaria solani. Llex cornuta endophyte separated from the llex cornuta living bodies in Hangzhou of Zhejiang province by an endophyte microorganism separation technology is identified as Streptomyces sioyaensis by microbial taxonomy and is named as Streptomyces sioyaensis 107. The Streptomyces sioyaensis 107 is preserved in the China general microbiological culture collection center on December 12, 2012 and has a preservation number of CGMCC No. 6984. Through liquid fermentation, the Streptomyces sioyaensis 107 can produce active metabolites having effects of inhibiting plant fungal disease pathogenic bacteria such as Colletotrichum lindemuthianum and Alternaria solani and is an important microbe resource in agriculture.

The invention relates to the technical field of microorganisms. An artemisia japonica endophytic fungus is collected from living bodies of artemisia japonica from a suburb of Guiyang city in Guiyang province through endophytic fungus separation technology. The artemisia japonica endophytic fungus is named pestalotiopsis uvicola GMH 31 through authentication by microbial taxonomy. The strain is deposited in China center for type culture collection with a deposition date being June-30-2014 and an accession number being CCTCC No: M2014303. The invention has following most significant characteristics: (1) A fermentation liquid of the strain is an active metabolite which has inhibiting effects of sclerotinia sclerotiorum, phytophthora capsici and the like and is an important microbial resource; and (2) A fermentation mycelium of the strain can secret various natural active components, has medicinal effects of brad-spectrum tumor resistance and the like, and is an important microbial resource of a novel natural active drug.

The invention discloses a yeast-like symbiote and use thereof. The yeast-like symbiote is obtained through being separated from a fat body of laodelphax striatellus by an in-vitro culture technology, is named Pichia anomala SP2014 through microbial taxonomic identification, has the collection number of CCTCC No: M 2014534, and is collected at the CCTCC (China Center for Type Culture Collection) at Oct. 17, 2014. Shown by research and analysis on the sensitivity of the yeast-like symbiote to different microbicides, two kinds of microbicides, namely Antracol and Nativo, with obvious inhibitory effects to P. anomala SP2014 are sprayed to wheat seedlings at the concentration of 800mg / L, the death rate reaches 46.7% and 63.3% respectively after the wheat seedlings are eaten by laodelphax striatellus, the death rate is remarkably higher than that of control, and the weight is remarkably lower than that of a control group. Proven by fluorescent quantitative detection results, the amount of yeast-like symbiotes in laodelphax striatellus bodies is remarkably reduced after the microbicides are used. The yeast-like symbiote can serve as an indication marker for monitoring vitality of laodelphax striatellus or be used for screening or producing pesticides for preventing and treating laodelphax striatellus.

The invention relates to the field of microbial technology and especially relates to a use of Streptomyces sioyaensis metabolite in prevention and control of Thanatephorus cucumeris. Llex cornuta endophyte separated from the llex cornuta living bodies in Hangzhou of Zhejiang province by an endophyte microorganism separation technology is identified as Streptomyces sioyaensis by microbial taxonomy and is named as Streptomyces sioyaensis 107. The Streptomyces sioyaensis 107 is preserved in the China general microbiological culture collection center on December 12, 2012 and has a preservation number of CGMCC No. 6984. Through liquid fermentation, the Streptomyces sioyaensis 107 can produce the active metabolites having effects of inhibiting plant fungal disease pathogenic bacteria such as Rhizoctonia solani and Thanatephorus cucumeris and is an important microbe resource in agriculture.

The invention discloses streptomycete strains and a combined application thereof in prevention and treatment of pepper seedling blight, and relates to the technical field of microorganisms. The streptomycetes disclosed by the invention are separated and obtained from a deep soil sample of 10-15cm collected from the Hangzhou xixi wet land in Zhejiang by adopting a streptomycete separation technology, are identified by microbial taxonomy, are named streptomyces albus (streptomyces albus) 2013 and streptomyces fradiae (streptomyces fradiae) M931,S.albus2013; the streptomyces albus 2013 is preserved at the China Center for Type Culture Collection on June 19, 2013; the preservation number is CCTCC NO:M2013269; the S.fradiae M931 is preserved at the China Center for Type Culture Collection on December 25, 2013; and the preservation number is CCTCC NO:M2013712. The two bacterial strains can be fermented to generate active substances with relatively strong antagonistic effects on rhizoctonia solani kuhn (Rhizoctonia solani Kuhn), and can be used for preventing and treating plant diseases such as the pepper seedling blight; when fermentation products of the two bacterial strains are mixed to prepare the mixed preparation according to the mass percent of 3 to 2, the effect for preventing and treating the pepper seedling blight is more significant in comparison with the effect when two metabolites are respectively used as single preparations.

The invention relates to the technical field of the microbial technology, an entophytic fungus is separated from strawberries and named Trichothecium roseum TR-1 through microbial taxonomy identification, and the strain is preserved in CCTCC (China center for type culture collection) (address: Wuhan University, Wuhan, China) with the preservation number of CCTCC NO:M 2015344. The Trichothecium roseum TR-1 is significantly characterized in that a strain liquid fermentation broth and an ethyl ether extract have the better inhabitation effect on the wheat powdery mildew. The excellent original strain is provided for further research and development of the Trichothecium roseum, and the strain is an important microbial resource in agriculture.

The invention relates to the field of microbial technologies, oleander endophytic fungi are obtained by adopting an endophytic fungi separation technology for separation in oleander living bodies collected in Zhejiang Academy of Agricultural Sciences, the endophytic fungi are named as nigrospora oryzae 46 through microbial taxonomy identification, a strain was collected in China General Microbiological Culture Collection Center on December 31, 2010, wherein the collection registration number is CGMCC No. 4509. The nigrospora oryzae 46 has the most significant characteristic that the strain subjected to liquid fermentation can generate active metabolites with inhibitory action for Rhizoctonia solani, Colletotrichumlindemuthianum, Botrytis cinerea and the like, thereby being an important microbial resource in the agriculture.

The invention relates to the field of microbial technology and especially relates to a use of Streptomyces sioyaensis metabolite in prevention and control of Rhizoctonia solani. Llex cornuta endophyte separated from the llex cornuta living bodies in Hangzhou of Zhejiang province by an endophyte microorganism separation technology is identified as Streptomyces sioyaensis by microbial taxonomy and is named as Streptomyces sioyaensis 107. The Streptomyces sioyaensis 107 is preserved in the China general microbiological culture collection center on December 12, 2012 and has a preservation number of CGMCC No. 6984. Through liquid fermentation, the Streptomyces sioyaensis 107 can produce the active metabolites having effects of inhibiting plant fungal disease pathogenic bacteria such as Rhizoctonia solani and Thanatephorus cucumeris and is an important microbe resource in agriculture.

The invention discloses an endophytic fungus in salvia miltiorrhiza bunge. The endophytic fungus is isolated and obtained from the living body of salvia miltiorrhiza bunge by adopting the endophytic fungus isolation and purification technology and is identified to be trichoderma atroviride through microbial taxonomy. The strain was collected in the China General Microbiological Culture Collection Center, with collection number being CGMCC No.4712.. Tanshinone compounds tanshinone I and tanshinone IIA are generated through liquid fermentation of the strain of the endophytic fungus in salvia miltiorrhiza bunge, so the endophytic fungus is an important microorganism for finding the new resources such as the tanshinone chemical components tanshinone I and tanshinone IIA.

The invention relates to the field of microbial technology and especially relates to a use of Streptomyces sioyaensis metabolite in prevention and control of Fusarium oxyspirum f. sp. niveum. Llex cornuta endophyte separated from the llex cornuta living bodies in Hangzhou of Zhejiang province by an endophyte microorganism separation technology is identified as Streptomyces sioyaensis by microbial taxonomy and is named as Streptomyces sioyaensis 107. The Streptomyces sioyaensis 107 is preserved in the China general microbiological culture collection center on December 12, 2012 and has a preservation number of CGMCC No. 6984. Through liquid fermentation, the Streptomyces sioyaensis 107 can produce the active metabolites having effects of inhibiting plant fungal disease pathogenic bacteria such as Rhizoctonia solani and Thanatephorus cucumeris and is an important microbe resource in agriculture.

The invention relates to the field of microbial technology and especially relates to a use of Streptomyces sioyaensis metabolite in prevention and control of Fusarium oxyspirum f. sp. niveum. Llex cornuta endophyte separated from the llex cornuta living bodies in Hangzhou of Zhejiang province by an endophyte microorganism separation technology is identified as Streptomyces sioyaensis by microbial taxonomy and is named as Streptomyces sioyaensis 107. The Streptomyces sioyaensis 107 is preserved in the China general microbiological culture collection center on December 12, 2012 and has a preservation number of CGMCC No. 6984. Through liquid fermentation, the Streptomyces sioyaensis 107 can produce the active metabolites having effects of inhibiting plant fungal disease pathogenic bacteria such as Rhizoctonia solani and Thanatephorus cucumeris and is an important microbe resource in agriculture.