34 results about "Thanatephorus cucumeris" patented technology

The invention relates to an agricultural sterilizing composite containing thifluzamide. The first active component is thifluzamide; the second active component is selected from kresoxim methyl, azoxystrobin, pyraclostrobin and phenylate kresoxim methyl; and the weight ratio of the first active component and the second active component is 16: 1 to 1: 16. The bactericide composite is used for preventing and treating the thanatephorus cucumeris and the rice false smut of the cereal crops, the thizoctonia cerealis, the rust disease, the loose kernel smut and the stinking smut, and has the advantages of reasonable components, treating and protecting functions, good sterilization effect, reduction of the usage frequency and low usage cost; and the activity and the sterilization effect of the bactericide composite are not the simple addition of the activities of the components. The invention has remarkable synergistic action and good safety to the crops, slows down the generation of the resistance and meets the safety requirements of the pesticide formulation.

This invention relates to molecular markers closely linked with Thanatephorus cucumeris resistance QTL, and their applications. The volumes of molecular markers gwm608, gwm88, wmc727 and gwm410.2 are 180bp, 130bp, 200bp and 300bp, respectively. The molecular markers can be used for detecting the genotypes of wheat variety CI12633 and its derived varieties or lines, and identifying Thanatephorus cucumeris resistant wheat varieties or lines. The molecular markers overcome the problems of long detection period and low efficiency faced by normal methods, and are rapid and highly efficient. Besides, the molecular markers also have such advantages as convenient resistance detection, no environmental influence, high accuracy and high efficiency.

The invention discloses an application of a distyrene-like compound containing thiazole ring as a sterilizing agent. A synthesized compound is subjected to antifungal activity testing as fusahum graminearum, mycosphaerella melonis, botrytis cinerea and thanatephorus cucumeris, testing structures can prove that (E)-4-(2,6-difluorophenyl)-2-substituted styryl thiazole compounds have certain inhibition activity for test targets, the inhibition rates of compounds Ic, If and Ii for fusahum graminearum are 50% or above, and the inhibition rates of compounds Id and Ii for mycosphaerella melonis are 55% or above, wherein the inhibition rate of the compound Id for mycosphaerella melonis is 61.6%, which shows medium inhibition activity.

The invention discloses bacillus velezensis Z002 as well as an acquisition method and application thereof. The bacillus velezensis Z002 is collected in China Center for Type Culture Collection (CCTCC), and the collection number is CCTCC No: M 2020567. The acquisition method comprises separation, screening and purification processes. Endophytic bacteria are firstly separated from tobacco plant tissue and subjected to dark culture, the endophytic bacteria antagonizing the tobacco mildew pathogen rhizopus oryzae are screened out, and finally purification is carried out to obtain the bacillus velezensis Z002. The application of the bacillus velezensis Z002 comprises fermentation, inoculation and modulation processes. Firstly, strains are cultured to obtain a fermentation inoculant, the fermentation inoculant is sprayed to tobacco petioles through a sprayer, tobacco leaves are put into a curing barn, and the tobacco leaves are cured according to a normal curing process. The strain bacillusvelezensis Z002 can effectively control tobacco mildew in the curing period, and the relative control effect is 80% or above. Secondly, the bacillus velezensis Z002 also has very good bacteriostatic effects on Ralstonia pseudosolanacearum, Phylophthora nicotianae, Botrytis cinerea, Sclerotinia sclerotiorum, Thanatephorus cucumeris, Fusarium oxysporum, Alternaria alternata and the like. The bacillus velezensis Z002 has good control effect and higher application and popularization value.

The invention relates to the technical field of biological prevention and control and bioengineering, in particular to bacillus amyloliquefaciens SY-SF-01 and application thereof. Bacillus amyloliquefaciens SY-SF-01 is preserved in China Center for Type Culture Collection, the preservation number is CCTCC M 2018264, and the preservation time is May fourteenth, 2018. The invention further providesa screening method of bacillus amyloliquefaciens SY-SF-01 and application of bacillus amyloliquefaciens SY-SF-01 in inhibition of rhizoctonia solani, cucumber fusarium wilt, thanatephorus cucumeris, red bean colletotrichum and verticillium dahliae kleb. A bacillus amyloliquefaciens SY-SF-01 strain has an extremely obvious prevention and control effect on rhizoctonia solani, the prevention rate forliving bodies reaches above 90%, the strain also has a good prevention and control effect on thanatephorus cucumeris, and the in-vitro antibacterial rate and the prevention rate for living potted plants can reach above 90%.

The invention relates to the technical field of microorganism. Garlic endophytic fungus disclosed by the invention is separated from a garlic living body collected from Zhejiang Hangzhou suburban gardens by the endophytic fungus microorganism separation technology and is identified by microorganism taxonomy. The garlic endophytic fungus is named as Aspergillus restrictus 28. The bacterial strain is collected in the common microorganism center of the China General Microbiological Culture Collection Center, the collection data is 31 December, 2010, and the collection registered number is CGMCC No.4507. The Aspergillus restrictus 28-generation product has the obvious characteristics that the bacterial strain is subjected to liquid fermentation to generate an active metabolism product which has an inhibiting effect on Rhizoctonia solani, Thanatephorus cucumeris and the like, and is an agricultural important microorganism resource.

The invention discloses applications of natural compound Murrayone as an agricultural bactericide. Natural compound Murrayone is taken as an agricultural bactericide used for preventing and treating agricultural fungal diseases, is capable of preventing and treating Sclerotinia sclerotiorum, Thanatephorus cucumeris, Botrytis cinerea, Fusarium graminearum, Colletotrichum gloesporioides, and Alternaria solani effectively, and possesses excellent treatment effects on fungal diseases such as Sclerotinia sclerotiorum, Thanatephorus cucumeris, and Colletotrichum gloesporioides. Agricultural bactericides prepared by taking natural compound Murrayone as an active ingredient possesses excellent curative effects, broad spectrum activity, and low toxicity, and are especially beneficial for environmental protection; no residue is left; and no pollution is caused.

The invention relates to the field of microbial technology. Chinese holly endophyte is separated through an endophyte microbe isolation technology from a Chinese holly plant living body collected from Hangzhou, Zhejiang province, is identified through microorganism taxonomy as Streptomyces sioyaensis, and is named as Streptomyces sioyaensis 107. The bacterial strain has already been conserved in China General Microbiological Culture Collection Center on the conservation date of December 12th, 2012 and the conservation register number of CGMCC No.6984. The most significant feature of the preparation method is that the bacterial strain liquid is fermented to produce an active metabolite which has an inhibition action on plant fungal disease pathogenic bacteria like Rhizoctonia solani and Thanatephorus cucumeris and is an important agricultural microbial resource.

The invention discloses a synthesis method of hydrogenated nopyl-containing symmetric genini quaternary ammonium iron and antibacterial application. The synthesis method comprises the following steps:performing heating reflux on hydrogenated nopyl-containing tertiary amine and alpha-omega-dibromohaloalkane as raw materials in an organic solvent, and performing cooling, crystallization, filtering,washing and vacuum drying after reactions, so as to obtain seven types of hydrogenated nopyl-containing symmetric genini quaternary ammonium iron. When the mass concentration of a medicine liquid is500mg / L, the seven types of hydrogenated nopyl-containing symmetric genini quaternary ammonium iron have good inhibition functions on ten plant pathogenic fungi, particularly the inhibition rates uponfusarium proliferatum, alternaria kikuchiana, ceratosphaeria phyllostachydis, sphaeropsis sapinea and fusicoccum aesculi are greatly higher than that of chlorothalonil upon the plant pathogenic fungi, and the inhibition rates of the hydrogenated nopyl-containing symmetric genini quaternary ammonium iron upon thanatephorus cucumeris is equivalent to that of chlorothalonil.

The invention relates to the field of microbial technology and especially relates to a use of Streptomyces sioyaensis metabolite in prevention and control of Thanatephorus cucumeris. Llex cornuta endophyte separated from the llex cornuta living bodies in Hangzhou of Zhejiang province by an endophyte microorganism separation technology is identified as Streptomyces sioyaensis by microbial taxonomy and is named as Streptomyces sioyaensis 107. The Streptomyces sioyaensis 107 is preserved in the China general microbiological culture collection center on December 12, 2012 and has a preservation number of CGMCC No. 6984. Through liquid fermentation, the Streptomyces sioyaensis 107 can produce the active metabolites having effects of inhibiting plant fungal disease pathogenic bacteria such as Rhizoctonia solani and Thanatephorus cucumeris and is an important microbe resource in agriculture.

The invention relates to the technical field of microorganisms. The garlic endophytic fungi disclosed by the invention is separated from a garlic plant living body collected in the suburban vegetable garden in Hangzhu city of Zhejiang province through use of endophytic fungus microorganism separation technology and is called after Aspergillus restrictus 28 through microbial taxonomic identification; and the strain is preserved in the General Microorganism Centre of China Microorganism Strain Preservation Management Committee on December the 31st, 2010 and has a preservation registered number of CGMCC No.4507. The strain liquid is obviously characterized by generating an active metabolic product which has the inhibiting effect on plant fungus disease pathogens, such as cucumber rhizoctoniasolani, rice thanatephorus cucumeris, and the like through fermenting and is an important microbial resource in agriculture.

The invention relates to an agricultural sterilizing composite containing thifluzamide. The first active component is thifluzamide; the second active component is selected from kresoxim methyl, azoxystrobin, pyraclostrobin and phenylate kresoxim methyl; and the weight ratio of the first active component and the second active component is 16: 1 to 1: 16. The bactericide composite is used for preventing and treating the thanatephorus cucumeris and the rice false smut of the cereal crops, the thizoctonia cerealis, the rust disease, the loose kernel smut and the stinking smut, and has the advantages of reasonable components, treating and protecting functions, good sterilization effect, reduction of the usage frequency and low usage cost; and the activity and the sterilization effect of the bactericide composite are not the simple addition of the activities of the components. The invention has remarkable synergistic action and good safety to the crops, slows down the generation of the resistance and meets the safety requirements of the pesticide formulation.

The invention discloses a diterpene compound extracted from branches and leaves of croton. The diterpene compound comprises 12-O-Tigloylphorbol-13-(2-methy lbutyrate) and 12-O-Acetylphorbol-13-(2-methy lbutyrate). The invention further discloses the use of the diterpene compound in preparing biopesticide for killing tobacco mosaic virus and phytopathogen. Experiments prove that the diterpene compound extracted from branches and leaves of croton, including the 12-O-Tigloylphorbol-13-(2-methy lbutyrate) and the 12-O-Acetylphorbol-13-(2-methy lbutyrate), can remarkably stop tobacco mosaic virus from invasion and proliferation and restrain cucumber wilt disease fungus, wheat scab pathogen, magnaporthe grisea, thanatephorus cucumeris, bipolaris maydis, exserohilum turcicum and other phytopathogens.

This invention relates to molecular markers closely linked with Thanatephorus cucumeris resistance QTL, and their applications. The volumes of molecular markers gwm608, gwm88, wmc727 and gwm410.2 are 180bp, 130bp, 200bp and 300bp, respectively. The molecular markers can be used for detecting the genotypes of wheat variety CI12633 and its derived varieties or lines, and identifying Thanatephorus cucumeris resistant wheat varieties or lines. The molecular markers overcome the problems of long detection period and low efficiency faced by normal methods, and are rapid and highly efficient. Besides, the molecular markers also have such advantages as convenient resistance detection, no environmental influence, high accuracy and high efficiency.

The invention relates to the field of microbial technology and especially relates to a use of Streptomyces sioyaensis metabolite in prevention and control of Rhizoctonia solani. Llex cornuta endophyte separated from the llex cornuta living bodies in Hangzhou of Zhejiang province by an endophyte microorganism separation technology is identified as Streptomyces sioyaensis by microbial taxonomy and is named as Streptomyces sioyaensis 107. The Streptomyces sioyaensis 107 is preserved in the China general microbiological culture collection center on December 12, 2012 and has a preservation number of CGMCC No. 6984. Through liquid fermentation, the Streptomyces sioyaensis 107 can produce the active metabolites having effects of inhibiting plant fungal disease pathogenic bacteria such as Rhizoctonia solani and Thanatephorus cucumeris and is an important microbe resource in agriculture.

The invention relates to the field of microbial technology and especially relates to a use of Streptomyces sioyaensis metabolite in prevention and control of Fusarium oxyspirum f. sp. niveum. Llex cornuta endophyte separated from the llex cornuta living bodies in Hangzhou of Zhejiang province by an endophyte microorganism separation technology is identified as Streptomyces sioyaensis by microbial taxonomy and is named as Streptomyces sioyaensis 107. The Streptomyces sioyaensis 107 is preserved in the China general microbiological culture collection center on December 12, 2012 and has a preservation number of CGMCC No. 6984. Through liquid fermentation, the Streptomyces sioyaensis 107 can produce the active metabolites having effects of inhibiting plant fungal disease pathogenic bacteria such as Rhizoctonia solani and Thanatephorus cucumeris and is an important microbe resource in agriculture.

The invention relates to the field of microbial technology and especially relates to a use of Streptomyces sioyaensis metabolite in prevention and control of Fusarium oxyspirum f. sp. niveum. Llex cornuta endophyte separated from the llex cornuta living bodies in Hangzhou of Zhejiang province by an endophyte microorganism separation technology is identified as Streptomyces sioyaensis by microbial taxonomy and is named as Streptomyces sioyaensis 107. The Streptomyces sioyaensis 107 is preserved in the China general microbiological culture collection center on December 12, 2012 and has a preservation number of CGMCC No. 6984. Through liquid fermentation, the Streptomyces sioyaensis 107 can produce the active metabolites having effects of inhibiting plant fungal disease pathogenic bacteria such as Rhizoctonia solani and Thanatephorus cucumeris and is an important microbe resource in agriculture.

The invention discloses a synthesis method and antibacterial application of a symmetrical gemini quaternary ammonium salt containing a hydrogenated nobyl group. The tertiary amine containing a hydrogenated nobyl group and α,ω-dibromohalogenated alkanes are used as raw materials and heated to reflux in an organic solvent to react After cooling, crystallization, filtration, washing, and vacuum drying, seven kinds of symmetrical gemini quaternary ammonium salts containing hydrogenated nobyl groups were obtained. At a liquid mass concentration of 500mg / L, seven kinds of quaternary ammonium salts containing hydrogenated nobyl groups have good inhibitory effects on ten kinds of plant pathogenic bacteria used, especially on Fusarium laminarum, Alternaria pears, Phyllostachys pubescens The inhibition rate of the fungus, pine blight fungus and Aescin sp. was much higher than that of chlorothalonil, and the inhibition rate of the rice sheath blight fungus was equivalent to that of chlorothalonil.

The invention relates to the microbial technology field. The garlic endophytic fungi are obtained from a garlic living body with endophytic fungi microbe separation technology, wherein the garlic living body is obtained at a vegetable garden in the suburb of Hangzhou city Zhejiang province. Through the microbe taxology identification, the garlic endophytic fungi are named as Aspergillus restrictus 28. The bacterial strain is preserved at China General Microbiological Culture Collection Center with a preservation date December 31st 2010 and a preservation registry number CGMCC No. 4507. The most substantial characteristic of the endophytic fungi is that through liquid fermentation the bacterial strain can produce an active metabolite which has an inhibition effect on pathogen of plant fungous disease such as Rhizoctonia solani, Thanatephorus cucumeris and the like. The bacterial strain is an important microbial resource.

The invention discloses a method for preventing and treating thanatephorus cucumeris. The method comprises the following steps of 1) conducting deep ploughing and upturned soil sunning on cultivated land from the late winter to the early spring, removing diseased plant residues and surrounding weeds in the cultivated land, uniformly spreading lime on the surface of the cultivated land after upturned soil sunning, harrowing the earth within 3 days, and continuously sunning upturned soil for more than 15 days after harrowing; and (2) after tobacco seedlings are transplanted, alternately spraying the following agents from the beginning of generation of disease spots, wherein the agents include a 10% validamycin aqueous solution, 30% benzoyl.propiconazole EC and 75% trifloxystrobin tebuconazole. According to the method, antibacterial treatment is carried out in two different stages from tobacco seedling transplanting to field planting stage management by combining the propagation characteristics of pathogenic bacteria of the thanatephorus cucumeris, deep ploughing, upturned soil sunning and disinfection are carried out on the cultivated land from the late winter to the early spring, the upturned soil sunning time is prolonged, and therefore pathogenic spores of the thanatephorus cucumeris can be better killed; and the different bactericidal agents are alternately applied after transplanting, so that drug resistance of tobacco plants is avoided, a good prevention and treatment effect is achieved, and the safety of tobacco planting is guaranteed.

The invention relates to the technical field of microorganism. Garlic endophytic fungus disclosed by the invention is separated from a garlic living body collected from Zhejiang Hangzhou suburban gardens by the endophytic fungus microorganism separation technology and is identified by microorganism taxonomy. The garlic endophytic fungus is named as Aspergillus restrictus 28. The bacterial strain is collected in the common microorganism center of the China General Microbiological Culture Collection Center, the collection data is 31 December, 2010, and the collection registered number is CGMCC No.4507. The Aspergillus restrictus 28-generation product has the obvious characteristics that the bacterial strain is subjected to liquid fermentation to generate an active metabolism product which has an inhibiting effect on Rhizoctonia solani, Thanatephorus cucumeris and the like, and is an agricultural important microorganism resource.

The invention discloses a diblock macromolecular quaternary ammonium salt containing a polyorganosiloxane chain block and preparation method and application thereof. The preparation method comprises the steps that polyorganosiloxane of which a single end is a vinyl group is firstly synthesized; the polyorganosiloxane of which the single end is a hydroxyl group is synthesized through the click reaction with mercaptoethanol; reaction between the polyorganosiloxane with the single ended hydroxyl group and alpha-bromoisobutyryl bromide is conducted to synthesize a polyorganosiloxane macromolecularevocating agent, and DMAPMA is evocated to conduct the polymerization of ATRP; finally benzyl chloride is used for conducting quaternization to synthesize the diblock macromolecular quaternary ammonium salt containing the polyorganosiloxane chain block. According to the diblock macromolecular quaternary ammonium salt containing the polyorganosiloxane chain block, the macromolecular quaternary ammonium salt which has amphipathy has good absorbing and infiltrating capabilities on sclerotium of thanatephorus cucumeris, and therefore the macromolecular quaternary ammonium salt has good inhibitingeffect on the germination of the sclerotium.

The invention relates to the field of microbial technology and especially relates to a use of Streptomyces sioyaensis metabolite in prevention and control of Fusarium oxysporum. sp. cucumebrium Owen. Llex cornuta endophyte separated from the llex cornuta living bodies in Hangzhou of Zhejiang province by an endophyte microorganism separation technology is identified as Streptomyces sioyaensis by microbial taxonomy and is named as Streptomyces sioyaensis 107. The Streptomyces sioyaensis 107 is preserved in the China general microbiological culture collection center on December 12, 2012 and has a preservation number of CGMCC No. 6984. Through liquid fermentation, the Streptomyces sioyaensis 107 can produce the active metabolites having effects of inhibiting plant fungal disease pathogenic bacteria such as Rhizoctonia solani and Thanatephorus cucumeris and is an important microbe resource in agriculture.

The invention discloses pesticidal bactericide containing bisultap and hexaconazole; the pesticidal bactericide contains active ingredients: bisultap and hexaconazole, having a weight ratio of 60:1 to 1:60. The pesticidal bactericide as a mixture is in the form of suspension, emulsion in water, wettable powder, water-dispersible granule and the like. The pesticidal bactericide can treat Cnaphalocrocis medinalis, Thanatephorus cucumeris, other rice diseases, and pest damage, can treat pest and disease damages by single application, fewer applications are required, the cost of applying bactericides to control crop harmful organisms can be effectively reduced, farmers feel less difficult to select pesticides, and the pesticidal bactericide is beneficial to organisms and environment and can protect the good ecological environment of farmlands.

The invention relates to the field of microbial technology and especially relates to a use of Streptomyces sioyaensis metabolite in prevention and control of Thanatephorus cucumeris. Llex cornuta endophyte separated from the llex cornuta living bodies in Hangzhou of Zhejiang province by an endophyte microorganism separation technology is identified as Streptomyces sioyaensis by microbial taxonomy and is named as Streptomyces sioyaensis 107. The Streptomyces sioyaensis 107 is preserved in the China general microbiological culture collection center on December 12, 2012 and has a preservation number of CGMCC No. 6984. Through liquid fermentation, the Streptomyces sioyaensis 107 can produce the active metabolites having effects of inhibiting plant fungal disease pathogenic bacteria such as Rhizoctonia solani and Thanatephorus cucumeris and is an important microbe resource in agriculture.

The invention relates to the field of microbial technology and especially relates to a use of Streptomyces sioyaensis metabolite in prevention and control of Botrytis cinerea. Llex cornuta endophyte separated from the llex cornuta living bodies in Hangzhou of Zhejiang province by an endophyte microorganism separation technology is identified as Streptomyces sioyaensis by microbial taxonomy and is named as Streptomyces sioyaensis 107. The Streptomyces sioyaensis 107 is preserved in the China general microbiological culture collection center on December 12, 2012 and has a preservation number of CGMCC No. 6984. Through liquid fermentation, the Streptomyces sioyaensis 107 can produce the active metabolites having effects of inhibiting plant fungal disease pathogenic bacteria such as Rhizoctonia solani and Thanatephorus cucumeris and is an important microbe resource in agriculture.