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Use of Streptomyces sioyaensis metabolite in prevention and control of Thanatephorus cucumeris

A technology of rice sheath blight bacteria and Streptomyces shioya, which is applied in the field of microorganisms and can solve problems such as insecurity

Inactive Publication Date: 2015-03-11
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide an endophytic actinomycete isolated and screened out from Chinese wolfberry for the deficiency that chemical pesticides are unsafe and the types of microbial source pesticides; Streptomyces sioyaensis (Streptomyces sioyaensis); Another object is to provide a method for utilizing the strain metabolites to prevent and treat plant fungal diseases

Method used

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  • Use of Streptomyces sioyaensis metabolite in prevention and control of Thanatephorus cucumeris
  • Use of Streptomyces sioyaensis metabolite in prevention and control of Thanatephorus cucumeris
  • Use of Streptomyces sioyaensis metabolite in prevention and control of Thanatephorus cucumeris

Examples

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Effect test

Embodiment 1

[0018] Example 1: (Isolation and Screening of Streptomyces Salthouse 107)

[0019] The present invention collected citric stalks in Hangzhou, Zhejiang in July 2012. After surface disinfection, endophyte separation, culture, fermentation, activity determination, and screening of active strains for inhibiting plant fungal disease pathogens, Streptomyces salina was obtained from it. 107, save.

[0020] Determination of antibacterial activity of Streptomyces saltrooms 107: Take 1mL of fermentation supernatant filtered by 0.22μm membrane in a sterile petri dish, and quickly mix it with 9mL of PDA medium cooled to 50℃, and then place it on each medium level after cooling. Put 1 rice sheath blight fungus (Thanatephorus cucumeris) or cucumber wilt fungus (Fusarium oxysporum.sp.cucumebrium Owen) or cucumber wilt fungus (Rhizoctonia solani) or watermelon wilt fungus (F.oxysporum f.sp. niveum) or Botrytis cinerea and other test bacteria cakes, the cakes are connected to the center of the pet...

Embodiment 2

[0023] Example 2: (Preparation method of fermentation metabolites of Streptomyces saltrooms 107)

[0024] Follow the steps below:

[0025] (1) Activation culture of strains: Transfer the isolated and preserved Streptomyces salina 107 strains to the potato dextrose PDA solid plate, and cultivate them at 28℃±1℃ until the slope of the test tube is full of spores for inoculation;

[0026] (2) Fermentation culture: Fermentation culture liquid contains 20g soybean meal powder, 15g corn flour, 10g bran, 5g peptone, (NH 4 ) 2 SO 4 5g, CaCO 3 5g, MgSO 4 10g, 60mL fermentation broth per 300mL triangular bottle; sterilize at 121°C for 20min after preparation, and then cool to 40°C. Pick a spore of Streptomyces haloplatinum 107 and inoculate it at 28°C±1°C. , The rotating speed of the shaker is 180 rpm, and the fermentation culture is 128h;

[0027] (3) Extraction of fermented product: After fermentation, the fermentation broth is collected by centrifugation, extracted with an equal volume of eth...

Embodiment 3

[0029] Example 3: (Inhibitory effect of metabolites of Streptomyces salina 107 on Rhizoctonia solani, Cucumber Rhizoctonia solani, Tomato Botrytis cinerea, Cucumber Fusarium wilt and Watermelon Fusarium wilt)

[0030] (1) Accurately weigh 0.20g of the metabolite of Streptomyces saltrooms 107, and add 10mL volume of sterile water, ultrasonically shake to fully dissolve, and finally prepare a mother liquor with a concentration of 20g / L;

[0031] (2) Determination of antibacterial activity: Dilute the above-mentioned mother liquor into 2g / L and 1g / L solutions respectively, take 1mL of each concentration solution in a sterile petri dish, and quickly mix with 9mL of PDA medium cooled to 50℃ Evenly (final concentration of the metabolites of Streptomyces salina 107 is 200mg / L and 100mg / L), and after cooling, place a 4mm diameter Rhizoctonia solani or Rhizoctonia solani or tomato ash on each medium plane. The fungus cake of mold fungus or cucumber Fusarium wilt or watermelon Fusarium wilt....

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Abstract

The invention relates to the field of microbial technology and especially relates to a use of Streptomyces sioyaensis metabolite in prevention and control of Thanatephorus cucumeris. Llex cornuta endophyte separated from the llex cornuta living bodies in Hangzhou of Zhejiang province by an endophyte microorganism separation technology is identified as Streptomyces sioyaensis by microbial taxonomy and is named as Streptomyces sioyaensis 107. The Streptomyces sioyaensis 107 is preserved in the China general microbiological culture collection center on December 12, 2012 and has a preservation number of CGMCC No. 6984. Through liquid fermentation, the Streptomyces sioyaensis 107 can produce the active metabolites having effects of inhibiting plant fungal disease pathogenic bacteria such as Rhizoctonia solani and Thanatephorus cucumeris and is an important microbe resource in agriculture.

Description

Technical field [0001] The present invention relates to the field of microbial technology, in particular to the cultivation of Streptomyces sioyaensis, an endophytic actinomycete for biocontrol, and the application of its metabolites. Background technique [0002] There are a large number of endophytes in plants. The endophytes were first found in pastures, and later in woody plants, herbs, grasses, moss, lichens, seaweeds and other plants. Studies so far have shown that plant endophytes can enhance the host’s disease resistance, increase plant productivity, and resist stress and insects. These effects are due to the endophytic bacteria multiplying and growing in the plant, and produce biologically active secondary metabolites in the body. Therefore, endophytes may become potential biological control agents in biological control, and have important uses in ecological agriculture and biological pesticide development. In recent years, plant endophytes have aroused widespread inte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/02A01P3/00
Inventor 俞晓平申屠旭萍汤谷刘叶丹陈昳丽俞叶微曾惠娟
Owner CHINA JILIANG UNIV
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