The invention relates to a sterilization composition containing a compound named as Chinese characters 'Fubenmixian'an' by the inventor and kasugamycin. The composition includes effective components which are the 'Fubenmixian'an' and the kasugamycin, with the balance being auxiliary components. The mass ratio of the 'Fubenmixian'an' to the kasugamycin in the composition is 1-50:50-1. A preparation includes 1-80% by mass of the effective components which are the kasugamycin and the 'Fubenmixian'an', with the balance being auxiliary components allowable and acceptable in pesticides. The dosage form of the composition is an emulsifiable solution, a suspension, wettable powder, water dispersible granules, an emulsion in water, a microemulsion, granules and microcapsules. The composition is mainly used for control of fruit tree rot, alternaria leaf spot, rice blast, soybean root rot, fusarium wilt of cucurbits, cotton verticillium wilt, jujube tree rust disease, grape white rot, ginseng and pseudo-ginseng black spot, tea gall of tea leaves, forest tree rot, canker, gummosis, leaf cast, forest seedling blight, vegetable soft rot pathogens, cucumber angular leaf spot pathogens, rice bacterial leaf blight pathogens, apple ring rot pathogens, wheat scab pathogens, and the like.