215 results about "Stripe rust" patented technology

Disclosed herein is a method for increasing the production of crops, particularly wheat and soybean, using herbicide resistant cultivars. In one aspect of this method, the method increases crop yield by diminishing the impact of the root diseases caused by Gaeumannomyces and Rhizoctonia species by treating the crop with an herbicide, in particular glyphosate. In another aspect the method for treating crops reduces the effects foliar pathogens and diseases, particularly fungal pathogens, such as rusts, including soybean rust, stem rust, stripe rust and leaf rust.

The invention discloses a filtration assisting method of yellow rust resistant wheat varieties and a specific primer. A filtration assisting primer pair of yellow rust resistant wheat varieties that is provided by the invention consists of a DNA that is shown in sequence 1 of the sequence table and a DNA that is shown in sequence 2 of the sequence table. The filtration assisting method of yellow rust resistant wheat varieties that is provided by the invention comprises the following steps: a genomic DNA of a wheat variety to be detected is used as a template, the primer pair is used for PCR augmentation, an augmented product is detected, and the wheat variety to be detected with a 343bp strip in the augmented product is yellow rust resistant wheat candidate. The invention further provides a molecular marker of a yellow rust resistance gene YrZH8 that is a DNA shown in sequence 3 of the sequence table. The molecular marker has closer linkage with YrZH84 and consequently has high efficiency in the breeding of yellow rust resistant wheat molecules and the cloning of the yellow rust resistance gene YrZH84. The primer pair and the molecular marker of the invention have important actions in the breeding of yellow rust resistant wheat varieties.

The invention discloses a method for screening a wheat variety with stripe rust resistance assisted by molecular markers and special primers used therein. Sequences of the primer pairs provided in the invention for assisted selection of a wheat variety with stripe rust resistance are described in a primer sequence table (Table 1). The method for assisted selection of a wheat variety with stripe rust resistance provided in the invention is carried out on the basis of conventional breeding and causes no interruption to the breeding process of a breeder except that in the generations of F2 and F6, after the selection of the breeder, candidate individual plants are subjected to molecular marker detection and only those individual plants containing two stripe rust resistant genes are reserved,thereby helping the breeder to more effectively select excellent strains containing a plurality of stripe rust resistant genes and enhancing endurance of stripe rust resistance of the strains. According to the invention, a novel stripe rust resistant gene Yrcaas and its special primers are discovered in the wheat variety Zhou 8425B; the gene is on wheat 1B chromosome and is located between SSR mark Xbarc8 and SSR mark Xgwm582, wherein the two marks can be used for assisted breeding of stripe rust resistant plants. The special primers of the stripe rust resistance genes in the invention are important to the breeding of stripe rust resistant wheat.

The invention discloses a composition of clorine-nalidixic-methyl and azole bactericides, the medical ingredients of which consist of the clorine-nalidixic-methyl and the azole bactericides. The composition has good and obvious bactericidal effect in preventing and treating crop diseases such as powdery mildew, banded sclerotial blight, stripe rust, root rot, rice blast, large and small leaf spot of corns, downy mildew, gray mold and sclerotinia, etc.

The present invention discloses method of screening out stripe rust resisting wheat and its special primer. The primer for screening out stripe rust resisting wheat includes the first primer pair comprising the nucleotide sequences of the sequence No. 1 and the sequence No. 2 and the second primer pair comprising the nucleotide sequences of the sequence No. 3 and the sequence No. 4 in the sequence list. The method of screening out stripe rust resisting wheat includes adopting the genome DNA of the tested wheat as template, performing PCR amplification under the guidance of the first primer pair and screening out amplified product with band of 265 bp size, performing PCR amplification under the guidance of the second primer pair and screening out amplified product with band of 238 bp size as the stripe rust resisting wheat variety. The SSR marks Xcfa2040 and Xbarc32 have the linkage distances of 1.4 cm and 4.8 cm separately. The present invention is significant in screening disease resisting wheat variety.

The invention relates to the fields of plant genetics and wheat breeding, in particular relates to a molecular marker for a wheat anti-stripe rust character, namely a wheat anti-strip rust gene Yr69 as well as a linked SSR molecular marker and an application method thereof, and aims at solving the problems that genetic distance between an existing molecular marker and the anti-strip rust gene Yr69 is long and marking is not accurate enough. The application method comprises the following steps: 1) amplifying by taking wheat DNA as a template and 2SA33 as a primer; 2) sequentially carrying out pre-degeneration, degeneration, annealing, extending, cycling, extending, and storing for later use; 3) carrying out electrophoresis, then mixing electrophoresis products with a loading buffer solution, then carrying out sample application, carrying out electrophoresis, and then dyeing and photographing; and 4) determining that Yr69 exists if a specific stripe is amplified. The invention has the advantages that firstly, the 2AS33 is obtained; and secondly, a dominant marker is adopted, the genetic distance is 1.9cm, the Yr69 gene is rapidly and accurately identified, powdery mildew resistance is predicted, and utilization of Yr69 is promoted.

The present invention provides wheat cultivars that are resistant to leaf rust and / or stripe rust disease caused by strains of the fungus Puccinia and to means and methods for producing same. Particularly, the present invention provides wheat cultivars comprising a chromosome segment of Ae. sharonensis, the chromosome segment confers, enhances, or otherwise facilitates resistance of the wheat plants to leaf rust and / or stripe rust disease.

The invention discloses a quick molecular detection method of a novel mycelium T4 injecting wheat stripe rust with AABBDDXX. The method comprises the following steps: screening to obtain two specific RAPD (Random Amplified Polymorphic DNA) marks S1311 and S1363 of T4 by use of 189 RAPD random primers among the novel mycelium T4, epidemic strains of wheat stripe rust CYR29, CYR31, CYR32, CYR33 and Su11-4 and a novel mycelium V26 injecting an anti-stripe rust gene Yr26; recovering, purifying, cloning and sequencing the specific stripes, designing an SCAR (Sequence Characterized Amplified Regions) primer according to the sequencing result, and successfully converting the RAPD marks to stable SCAR marks T4SP8-1 / T4SP8-2 (the sequence is 5'GGGCGTGGATGAAGAG-3' / 5'-GGAGCAGAAGCAGGTTT3'); stably amplifying the specific stripe of the novel mycelium T4 at 700bp by the marks. The specific SCAR marks T4SP8-1 / T4SP8-2 of the novel mycelium T4 provided by the invention can be used for realizing quick molecular detection of the novel mycelium T4 in an early stage in a large field so as to understand the variation dynamic condition of the pathogenic bacteria, thereby providing a reliable technical support and a theoretical foundation for prophylaxis and treatment policy of the mycelium and the wheat stripe rust.

The present invention discloses a method for breeding multigene polymerized wheat germplasm capable of resisting powdery mildew, stripe rust and verticillium wilt. Said method includes the following steps: utilizing STS marker of Pm4 to detect that the wheat to be detected has the gene Pm4a for resisting powdery mildew or not; utilizing SCAR marker of Pm13 to detect that the wheat to be detected has the gene Pm13 capable of resisting powdery mildew or not; utilizing SCAR marker of Pm21 to detect that the wheat to be detected has the gene PmV capable of resisting powdery mildew or not; utilizing SCAR marker of Bdv2 to detect that the wheat to be detected has the gene Bdv2 for resisting verticillium wilt or not; and utilizing SSR marker of YrX to detect that the wheat to be detected has the gene YrX for resisting stripe rust or not. Said invention provides a new method for breeding new wheat variety capable of resisting powdery mildew, stripe rust and verticillium wilt.