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Molecular marker interlocking with wheat stripe rust resistant gene, acquisition method and application thereof

A technology for molecular markers and stripe rust, which is applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve the problems of distant target genes, few applications, and limited versatility

Active Publication Date: 2013-05-01
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the versatility of stripe rust resistance gene linkage markers obtained based on a single or a small number of isolated populations is often limited by the variety background; moreover, most markers are far away from the target gene, so these markers are not widely used in breeding

Method used

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  • Molecular marker interlocking with wheat stripe rust resistant gene, acquisition method and application thereof
  • Molecular marker interlocking with wheat stripe rust resistant gene, acquisition method and application thereof
  • Molecular marker interlocking with wheat stripe rust resistant gene, acquisition method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1: RGAP molecular marker Xwgp linked to wheat stripe rust resistance gene -160bp the acquisition

[0057] 1. Test materials

[0058] Plant materials: The susceptible variety Taichang 29 was used as the female parent, and the new disease-resistant germplasm HRMSN-81 was used as the male parent to construct F 2 Genetic Mapping Populations. In the main season of 2010, F 2 148 individual plants of the population were planted in the disease identification nursery of Wenjiang Experimental Base, Sichuan Agricultural University, Chengdu Campus, for resistance identification. Some leaves of each individual plant were taken at the seedling stage for RGAP analysis. When ripe, harvest F 2:3 The seeds of the family were dried and preserved, and used for resistance identification in 2011.

[0059] Mixed Physiological Races of Stripe Rust: For F 2 group and F 2:3 The mixed physiological races of stripe rust identified by family resistance consisted of equal mixtures of...

Embodiment 2

[0086] Example 2: Chromosomal location of specific RGAP molecular marker Xwgp-160bp

[0087] 1. Test materials

[0088] Chinese spring, Chinese spring deficiency-quadromic (including: N1AT1D, N1BT1D, N1DT1B, N2AT2D, N2BT2D, N2DT2A, N3AT3D, N3BT3D, N3DT3A, N4AT4D, N5AT5D, N5DT5A, N6AT6D, N6BT6D, N7AT7D, N7BT7D, N7DT7A) and 2D chromosome Diendosomal (Dt2DS and Dt2DL) genetic material.

[0089] 2. Genomic DNA extraction is the same as in Example 1.

[0090] 3. Specific molecular marker Xwgp -160bp RGAP analysis

[0091] Screening a Molecular Marker Xwgp Linked to Wheat Stripe Rust Resistance Gene -160bp , obtained by amplifying the similar sequences of disease-resistant genes with degenerate primers (upstream primer: 5'-AAGTGGAACAAGGTTACG-3'; downstream primer: 5'-GGIGGIGTIGGIAAIACIAC-3'). Using this pair of degenerate primers, RGAP analysis was performed on the above-mentioned Chinese spring, Chinese spring deletion-tetrasomy and 2D chromosome double-telosome genetic materi...

Embodiment 3

[0094] Example 3: Molecular-assisted selection of wheat stripe rust resistance gene

[0095] 1. Test materials

[0096] Plant materials: the susceptible variety Taichang 29, the new disease-resistant germplasm HRMSN-81, the advanced generation (F 5 ) 5 wheat lines (L1-L5), 11 wheat varieties that have no genetic relationship with the disease-resistant variety HRMSN-81, including: Neimai 8, Neimai 9, Kechengmai 2, Chuanmai 47 , Chuanmai 42, Chuannong 16, Mianmai 4, Mianyang 3, Mianyang 26, Mianyang 29, and Chuanfu 5. In the first season of 2011, the above materials were planted in the disease identification nursery of Wenjiang Experimental Base, Sichuan Agricultural University, Chengdu Campus, for resistance identification. Some leaves of each individual plant were taken at the seedling stage for RGAP analysis.

[0097] Mixed physiological race of stripe rust: The mixed physiological race of stripe rust used for resistance identification is composed of three races 31, 32 and...

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Abstract

The invention relates to the fields of agricultural biotechnical engineering and wheat disease resistance genetic breeding, and in particular relates to a molecular marker interlocking with a wheat stripe rust resistant gene, an acquisition method and application thereof. The marker interlocking with the wheat stripe rust resistant gene is obtained through amplification by degenerate primers: an upstream primer 5-'AAGTGGAACAAGGTTACG-3', and a downstream primer 5'-GGIGGIGTIGGIAAIACIAC-3'. The marker can be used to assist selective breeding of wheat stripe rust resistance, and overcome the shortcomings in wheat stripe rust resistance identification in conventional breeding, such as vulnerability to environment impact, poor stability and repeatability, heavy workload, time and labor consumption and long breeding cycle, so as to speed up the breeding process of a wheat stripe rust resistance variety.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology engineering and wheat disease-resistant genetic breeding, in particular to a molecular marker linked with a wheat stripe rust-resistant gene, its obtaining method and application. Background technique [0002] Wheat stripe rust caused by Puccinia striiformis West.f.sp.tritici (Pst) is a worldwide airborne leaf disease. It is prevalent in production areas, especially in cold or high-altitude areas, causing serious damage to wheat production. China is the largest and relatively independent wheat stripe rust endemic area in the world. The emergence and prevalence of new virulent races have exacerbated the trend of stripe rust, making China's wheat once again under the threat of stripe rust pandemic. Therefore, it is urgent to find and introduce new disease resistance genes to enhance the resistance of wheat cultivars to meet production needs. [0003] The discovery and identification of n...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 郑有良陈国跃陈时盛魏育明刘亚西蒲至恩邓梅
Owner SICHUAN AGRI UNIV
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