The invention discloses a cutting propagation method of eustoma grandiflorum. The cutting propagation method includes: day temperature of a female parent cultivation greenhouse is kept at 20-30 DEG C, night temperature is kept at 13-18 DEG C, relative air humidity during the day is kept to be 50-75%, and relative air humidity during the night is kept to be 70-85%; and sunshine time in a year is less than 13 hours. When a lateral bud of a female parent plant has eight leaves and one heart, a bigslip is cut out, cutting is performed on the bigslip after the bigslip is dipped in rooting powder, shading coefficient of an outer shading net of a cutting greenhouse is 30%, inner relative air humidity is kept to be 80-95% through high pressure mist, temperature is at 20-25 DEG C, CO2 concentration in the cutting greenhouse is kept to be 600-800ppm through CO2 fertilization, and a cutting seedling is transplanted to a flower cutting production greenhouse after the cutting seedling takes root. The cutting propagation method of eustoma grandiflorum reduces root disease rate and leaf disease rate substantially, shortens seed breeding stage, and promotes the eustoma grandiflorum pigslip to take root. Rooting time of eustoma grandiflorum is 23-25 days, rooting rate of eustoma grandiflorum is above 92%, and planting percent of eustoma grandiflorum is above 87%. The cutting propagation method of eustoma grandiflorum is suitable for commercial and large-scale propagation of eustoma grandiflorum.