210results about How to "High sporulation" patented technology

The invention discloses a novel strain CLIMAX-VSF of streptomyces fradiae. The preservation number of the novel strain is CGMCC No. 5133; the preservation date is August 12, 2011; and the preservation department is China General Microbiological Culture Collection Center. The invention further discloses a microbial inoculum containing the novel strain. The novel strain CLIMAX-VSF of the streptomyces fradiae, which is provided by the invention, has quite obvious performances of disease resistance, nitrogen fixation, phosphate solubilization and somatotropin secretion. By using the strain, the germination rate and the growth speed in a seedling stage can be obviously improved under the condition that nutrients are not added to soil, and furthermore, diseases in the seedling stage and a growth period are obviously inhibited. On the aspect that the strain is used for the mixed preparation and the synergy of a fertilizer, the strain shows to have the favorable effect of activating soil nutrients and the performance of resisting a plurality of cultivation obstacles.

The invention discloses a multifunctional high efficient organic material decomposition agent and a preparation method thereof. The decomposition agent of the invention comprises composite original strains consisting of bacillus amyloliquefaciens, Aspergillus oryzae, Trichoderma viride, and Saccharomyces cerevisiae and an organic material carrier, and an inorganic carrier. Strains of the decomposition agent can be mutualistic, and have complementary advantages. The adaptation is wide, and a plurality of organic matter clastic enzymes can be produced by metabolism. The decomposition agent has high activity and can be used for fermentation and conversion of a plurality of organic materials such as livestock and poultry excrements, crop straws, city sludge, food waste, food processing and pharmaceutical waste residues, and has fast fermentation start, fast deodorization, low temperature resistance, and low moisture resistance. The fermentation period is shorter by 5-7 days than that in the same technology. Fermented and converted organic fertilizers have the obvious rhizosphere ecological regulation and control function, promote the root growth, and improve the root vitality. A plurality of soil borne diseases are prevented by improving the flora diversity, hyperparasitism and biological antagonism.

The invention discloses streptomyces albidoflavus strain and an application thereof to control cucumber downy mildew, and belongs to the field of biological control on plant diseases. The bacterial strain is streptomyces albidoflavus ACT033, and the preservation number is CGMCC No. 8125. The invention also discloses a biological control preparation which is prepared by employing the new streptomyces albidoflavus strain ACT033 and is capable of effectively controlling cucumber downy mildew and a preparation method of the preparation. Experiments show that the streptomyces albidoflavus strain ACT033 of the invention has the characteristics of fast growth speed, large sporulation quantity, broad-spectrum effect, strong stress resistance and the like, and can be fast colonized in quantity at the plant roots, so good application prospect is achieved. The biological control preparation prepared from the streptomyces albidoflavus strain ACT033 not only can control cucumber downy mildew, but also can effectively increase crop output.

The invention relates to a Beaueria bassaria (Balsamo) Vuillemin bacterial strain GZGY-1-3, a culture method of the bacterial strain, spore powder prepared from the bacterial strain and application of the spore powder in preparing a biocontrol agent, wherein the biocontrol agent can be used for preventing and controlling bemisia tabaci, frankliniella occidentalis, green onion aphidand / or green peach aphid. The biocontrol agent can be used for preventing and controlling insects under a high temperature environment, and is good in prevention and control effect with field control efficiency over 75%.

The invention relates to a method for producing a fungal inoculant, and particularly relates to a method for producing an AM fungal inoculant. The method is characterized by comprising the following steps: carrying out pregermination, inoculation, seedling-stage management and inoculant quality analysis on a corn; and 4 months after inoculation, collecting the root system of a host plant, and inquiring the dip dyeing station and dip dyeing rate of AM fungi by adopting Phillips and Hayman dyeing methods for the quality evaluation of an inoculant. The method disclosed by the invention has the advantages of uniqueness, high inoculant quality and high dip dyeing rate.

The invention discloses gene recombination bacillus amyloliquefaciens and a microbial inoculum preparation method. A bacillus amyloliquefaciens FZB42 strain is taken as a host bacterium to construct the gene recombination bacillus amyloliquefaciens XLV09-1 with transparent vitreoscilla hemoglobin which is regulated and controlled by a P43 strong promoter by contrasting a gene recombination vector. The produced spore number of microbial inoculum prepared by fermenting the bacillus amyloliquefaciens can reach more than 100 hundred millions per milliliter, the overall yield of antibacterial lipopeptid reaches more than 1 gram per liter and prevention effect on various fungal plant diseases is improved by over 30 percent. The microbial inoculum is a green microecological preparation, of no toxic residue, safe to human and livestock, and favorable for protecting the ecological environment.

The invention provides a Trichoderma viride strain and application thereof. TheTrichoderma viride provided by the invention has a preservation number of CGMCC No.5044, and characteristics of fast growth, large sporulation capacity, wide action spectrum, strong stress resistance, and rapid colonization in large quantity in rhizosphere of plants, and can be used for the prevention and control of plant soil-borne diseases. A biological control preparation prepared from the Trichoderma viride can not only efficiently control the soil-borne plant diseases but also effectively increase the yield of crops, and has promising application prospects.

The invention relates to a Trichoderma strain for antagonizing soil-borne disease, belonging to the field of biological control. The Trichoderma strain is Trichoderma asperellum ZJSX5003 CGMCC No.6480. The Trichoderma strain provided by the invention has the characteristics of high growth speed and high spore production amount; the in-vitro standoff pathogen inhibition rates are respectively up to 79.79%, 73.68% and 72.25%, the pathogen spore survival rates are respectively 0.00%, the in-vivo preventive effects for cucumber fusarium wilt and maize stem rot are respectively 85.08% and 66.67%, the enzyme activity of chitinase is 4.0783U, the enzyme activity of beta-1,3-glucanase is 0.8050U, the enzyme activity of extracellular proteinase is 4.8503U, and the content of silicone, terpene and carboxylic acid having antagonistic actions in antibiotic secondary metabolites is 44.44%. The Trichoderma strain provided by the invention has the advantages of high antagonism and high specificity. The biological pesticide produced from the strain can effectively control the soil-borne disease, thereby ensuring the stable increase of crop and vegetable yields.

The invention discloses bacillus velezensis and application thereof in prevention and control of watermelon fusarium wilt. The strain is a bacillus velezensis strain XT1-3, is obtained by being separated from the lake mud of the Dongping Lake in Tai'an City of Shandong province, is preserved in China General Microbiological Culture Collection Center (CGMCC) on September 26, 2017, and has a preservation number of CGMCC No. 14746. Test results prove that the bacillus velezensis strain XT1-3 biological control preparation is remarkable in prevention and control effect of the watermelon fusarium wilt, and the prevention and control effect reaches 77.03%; furthermore, after the bacillus velezensis is used, watermelons grow robustly and are protected from pesticide hazard.

The invention relates to application of a biocontrol fungus Trichoderma virens SS161 and metabolites thereof, belonging to the technical field of microorganisms. The collection number of the biocontrol fungus Trichoderma virens SS161 provided by the invention is CGMCC No.7224. The strain is separated from a rotten rice straw in a rice seedling breeding disc, and shows high antagonistic action on multiple pathogenetic fungi. A fermentation product of the strain has growth promoting action on rice and contains antibacterial substances. The separation of the strain provides an excellent original strain for the research and development of microorganism-derived agricultural chemicals.

The invention discloses a separation and cultivation method for isaria fumosorosea. The method comprises the following steps of collecting bemisia tabaci bodies infected with entomogenous fungus from vegetable leaves, separating the entomogenous fungus from the bodies, putting the entomogenous fungus on a PDA (potato dextrose agar) culture medium for cultivating to obtain spore suspension which is sprayed on two-year-old nymphs of bemisia tabaci, then separating the entomogenous fungus from the bodies again after cultivation, storing the entomogenous fungus on a slope of a test tube and storing the test tube in a refrigerator; taking the entomogenous fungus out of the refrigerator, preparing the spore suspension, dipping the spore suspension through filtration paper, inoculating the spore suspension onto the culture medium, cultivating in a mildew incubator, picking up hypha, inoculating the hypha into germfree water, then stirring and filtering; putting the spore suspension at the center of a culture dish, perforating a hole in the center of a bacterial colony, dispersing tween-80, stirring and filtering again, and calculating the sporulation quantity and the diameter of the bacterial colony. The method is used for screening an optimal culture medium formula, the optimal temperature, an optimal pH, a lighting condition and a ventilating condition. The method has the advantages that the diameter area of the bacterial colony is large and the sporulation yield is large.

The invention belongs to the field of microbial fermentation and in particular relates to a method for preparing taste-active peptides and delicious amino acids by using aspergillus oryzae fermented peanut meal and application thereof. The method comprises the following steps: performing ultraviolet mutagenesis by utilizing aspergillus oryzae AS 3.95, and screening to obtain a mutant strain of which the protease activity is improved; and performing peanut meal fermentation by utilizing the strain so as to produce the peanut peptides and delicious amino acids with good flavors. The hydrolysis rate and delicate flavor value of the peanut proteins can be improved, the content of taste-active peptides and free amino acids is increased, and the method has high application value in flavoring base materials. The main component of the selected fermentation medium is peanut meal, the raw materials are easily available and low in price, and the cost is low. The hydrolysis rate and delicate flavor value of the peanut meal proteins can be obviously improved after fermentation, and the method has high application value. According to the preparation method disclosed by the invention, the fermentation cost can be reduced, the production link is simplified, and the efficiency is improved.

The present invention relates to a kind of Trichoderma harzianum strain, belongs to the field of microbiological technology. The Trichoderma harzianum strain, named as Trichoderma harzianum B, is preserved in the preservation number of CGMCC No. 0955, and belongs to Moniliaaceae, Hyphomycetales, Hyphomycetidae of Deuteromycotina. The Trichoderma harzianum strain of the present invention has high spore yield and high efficiency wide spectrum plant disease preventing capacity, and may be used to promote plant growth and suitable for industrial production.

The invention relates to a trichoderma atroviride strain for producing myrosase and application thereof. The file number of the dark-green trichoderma atroviride strain for producing myrosase is CGMCC (China general microbiological culture collection) No.5609. During use, trichoderma atroviride is applied to soil containing brassica plant tissue as fumigated materials in a solid bactericide manner, pests in the soil are killed by biological fumigation, and pest diseases spread by the crop soil are prevented.

The invention belongs to the technical field of microorganism plant growth promotion, and particularly relates to Trichoderma viride, a fungicide, a biological fertilizer and application. The biological organic fertilizer uses industrial waste as the raw material, the environment pollution problem is effectively solved through functional microorganisms, and the effects of reducing crop diseases and improving the yield are realized. The Trichoderma viride biological organic fertilizer has the multiplex effects of promoting growth, increasing the yield and preventing diseases and has the good development significance. The Trichoderma viride Tv-1511 has the good effect of promoting seed germination and seedling growth, a solid fermentation material obtained after the Trichoderma viride is inoculated in mushroom residues can serve as the good biological organic fertilizer, and the Trichoderma viride is convenient to use and remarkable in growth promoting and yield increasing effect and hasbroad development prospects.

The invention provides a streptomyces fradiae solid culture medium, a culture method and a preparation method of an agricultural microbial agent, belonging to the field of microbial fermentation. The streptomyces fradiae solid culture medium is prepared from the following components in parts by weight: 3-10 parts of corn flour, 3-18 parts of soybean meal, 18-45 parts of straw powder, 10-30 parts of turfy soil, 20-50 parts of phosphate tailing, 1-10 parts of calcium carbonate and 40-50 parts of nutrient solution. According to the invention, a solid fermentation culture medium is prepared through liquid-solid two-phase fermentation by adopting the solid culture medium and is used for producing an agricultural streptomyces fradiae agent. The fermentation culture medium for streptomyces fradiae spore production, provided by the invention, is suitable for the growth of streptomyces fradiae, and over 7.5*10<9> spores can be produced from each gram of fermentation raw material; and the germination rate of the prepared spore powder can reach 95%, the prepared agricultural microbial agent conforms to the national standard of agricultural microbial agent, and an obvious promotion effect is realized on the crop growth.

The invention discloses a bacillus pumilus strain and an application thereof in apple alternaria leaf spot prevention and control. The strain is the bacillus pumilus strain QZ-002H, and the preservation number is CGMCC No.11050. The invention further discloses a biocontrol preparation containing the bacillus pumilus strain QZ-002H and a preparation method thereof. Experiments prove that the bacillus pumilus strain has the advantages of being rapid in growth rate, large in sporulation quantity, high in stress resistance, capable of being rapidly colonized in plant rhizosphere in large quantities and the like, and therefore a good application prospect is achieved. The biocontrol preparation prepared from the bacillus pumilus can effectively prevent and control apple alternaria leaf spot.

The invention discloses a salt-tolerant trichoderma pleuroticola strain and application thereof, belonging to the technical field of dephosphorization microbial fertilizers. The strain is trichoderma pleuroticola HF01 and has the collection number of CGMCC (China General Microbiological Culture Collection Center) No. 8127. The invention further discloses a microbial agent containing the strain and a preparation method thereof. Shown by experiments, the trichoderma pleuroticola strain disclosed by the invention has the characteristics that the growth speed is high, the spore yield is large, the salt tolerance is strong, the stress resistance is strong, the rapid and large-number colonization at the rhizosphere of plants can be achieved, and the like; the strain has broad application prospects in the aspects of increasing the phosphate fertilizer utilization ratio, improving the soil structure and increasing the yield and quality of crops.

The invention discloses bacillus methylotrophicus as well as a biological prevention and treatment preparation and applications of the bacillus methylotrophicus, belonging to the technical field of biological prevention and treatment on plant diseases. The bacterial strain is preserved in the China General Microbiological Culture Collection Center, and is assigned with the accession number of CGMCC No.12218. The experiment proves that the bacillus methylotrophicus strain ZDC-03 has the characteristics of being rapid in growth, high in sporulation quantity, and strong in adverse resistance, and can be rapidly colonized in large quantities, and therefore, the bacillus methylotrophicus strain ZDC-03 has good application prospects. The biological prevention and treatment preparation prepared by adopting the bacillus methylotrophicus can be used for efficiently preventing and treating plant diseases caused by botryis cinerea, and also can be used for effectively improving the crop yield, thus being the biological prevention and treatment preparation with extremely good application prospect. The biological prevention and treatment preparation prepared by adopting the bacillus methylotrophicus can be used for preventing and treating the plant diseases caused by botryis cinerea.

The invention discloses a Trichoderma viride strain and application thereof. The Trichoderma viride stain is WLTR35, and the collection number is CGMCC No.6673. The invention also discloses application of the strain or biological control preparation comprising the strain in controlling plant soil-borne disease. The experiment indicates that the Trichoderma viride strain WLTR35 has the characteristics of fast growth, large spore production quantity, wide action spectrum and the like, can quickly and massively implement colonization, and therefore, has wide application prospects. The biological control preparation prepared from the Trichoderma viride WLTR35 can efficiently control the plant soil-borne disease and effectively enhance the crop yield.

The invention discloses a bacillus safensis strain ZDC-02, and belongs to the technical field of biological control of plant diseases, wherein the strain is preserved in China General Microbiological Culture Collection Center with preservation number of CGMCC No.12217. Experiments indicate that the bacillus safensis strain ZDC-02 has the characteristics of being rapid in growth speed, broad in action spectrum, strong in stress resistance, capable of achieving rapid mass colonization on plant bodies and the like; therefore, the strain has a good application prospect. A bio-control preparation, as a bio-control preparation which has a quite broad application prospect and is prepared from the bacillus safensis, not only can effectively prevent and control various plant diseases but also can effectively improve a crop yield.

The invention relates to the technical field of microbial agents, in particular to bacillus megatherium T317, a microbial agent and a preparation method of the microbial agent. The bacillus megatherium T317 is collected in China Center for Type Culture Collection, and the collection number is CCTCCM 2015753. The preparation method of the microbial agent comprises the following steps of (1) separating and sieving; (2) purifying and storing; (3) solid fermenting and culturing; (4) preparation of microbial agent, so as to obtain the microbial agent of the bacillus megatherium T317. The microbial agent has the advantages that the bacillus megatherium T317 is used as an original strain, and the nitrogenase activity and the auxin secreting capability are measured; the bacillus megatherium T317 has higher nitrogenase activity and IAA (indole acetic acid) secreting capability, the nitrogenase activity of bacillus megatherium T317 is 800-900nmol / (mL h), and the IAA secreting amount is 300-400mg / L in the growth and metabolism process.