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Bacillus methylotrophicus as well as biological prevention and treatment preparation and applications thereof

A biological control preparation, methyl nutritional technology, applied in the directions of biocides, microorganism-based methods, biochemical equipment and methods, etc., can solve problems such as environmental pollution residues, achieve efficient control, increase crop yield, and improve The effect of the application foreground

Inactive Publication Date: 2016-11-16
青岛中达农业生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a methylotrophic bacillus and its biological control preparation and application, which can solve the problems of environmental pollution and residues caused by the use of chemical agents

Method used

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  • Bacillus methylotrophicus as well as biological prevention and treatment preparation and applications thereof
  • Bacillus methylotrophicus as well as biological prevention and treatment preparation and applications thereof
  • Bacillus methylotrophicus as well as biological prevention and treatment preparation and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0021] 1. Isolation and purification of Bacillus methylotrophicus ZDC-03

[0022] The methylotrophic Bacillus (Bacillus methylotrophicus) ZDC-03 of the present invention is obtained by separating and obtaining by streaking on a plate, and the specific separation method is as follows: weigh 1 g of the soil sample in 99 mL of sterile water, place it on a shaker for 5-10 min, Mix the soil sample with water thoroughly to get 10 -2 Soil dilution. Take 1ml of the above soil dilution solution to 9ml sterile water, shake well to obtain 10 -3 Soil dilution. Configure 10 in the same way -4 、10 -5 、10 -6 soil dilution. Use an inoculation loop to draw soil dilutions of different concentrations and draw a line on the plate, and each concentration is repeated 3 times. The petri dish was placed in an incubator at 28-30°C for 24 hours, and the colonies of different forms on the culture medium were streaked on a blank NA petri dish, and the Bacillus strains were purified and stored unde...

Embodiment 2

[0028] Example 2 Verification of indoor effect of methylotrophic bacillus strain ZDC-03 on tomato gray mold

[0029] 1. Experimental method

[0030] Using the plate confrontation culture method, move the tested tomato gray mold (Botrytis cinerea) bacterium cake (8mm) to the center of the plate, inoculate the ZDC-03 bacterial strain at 2cm around it, and place it in a constant temperature incubator at 28-30°C for cultivation. The culture dish not inoculated with Bacillus was used as the control, and each treatment was repeated 3 times. When the control hyphae covered the plate, the diameter of the colony and the diameter of the inhibition zone were measured by the cross method, and the average value and relative inhibition rate were calculated.

[0031] 2. Results

[0032] It can be seen from Table 1 that the methylotrophic Bacillus strain ZDC-03 has a strong inhibitory effect on the mycelial growth of Botrytis cinerea. The diameter of the inhibition zone is 1.45 cm, and the ...

example 3

[0035] Example 3 Field effect verification of methylotrophic bacillus ZDC-03 fermented liquid on tomato botrytis cinerea

[0036] 1.1 Drugs to be tested

[0037] Methylotrophic Bacillus ZDC-03 fermentation broth; 50% procymidone wettable powder (commercially available).

[0038] 1.2 Test crops and control objects:

[0039] Test crop: tomato, variety Nongbo Fenba No. 3

[0040] Control object: Botrytis cinerea

[0041] 1.3 Conditions of the test site

[0042] The test site is located in the tomato protection area of ​​Shouguang City, Shandong Province. In 2015, the tomato gray mold control experiment was carried out in the greenhouse of the tomato protection area in Shouguang City. The cultivation conditions (soil type, amount of fertilization, watering, etc.) of all experimental plots were uniform and in line with the actual agricultural production in the area.

[0043] 1.4 Experimental Design and Arrangement

[0044] This experiment designed 400 times, 600 times, 800 ti...

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Abstract

The invention discloses bacillus methylotrophicus as well as a biological prevention and treatment preparation and applications of the bacillus methylotrophicus, belonging to the technical field of biological prevention and treatment on plant diseases. The bacterial strain is preserved in the China General Microbiological Culture Collection Center, and is assigned with the accession number of CGMCC No.12218. The experiment proves that the bacillus methylotrophicus strain ZDC-03 has the characteristics of being rapid in growth, high in sporulation quantity, and strong in adverse resistance, and can be rapidly colonized in large quantities, and therefore, the bacillus methylotrophicus strain ZDC-03 has good application prospects. The biological prevention and treatment preparation prepared by adopting the bacillus methylotrophicus can be used for efficiently preventing and treating plant diseases caused by botryis cinerea, and also can be used for effectively improving the crop yield, thus being the biological prevention and treatment preparation with extremely good application prospect. The biological prevention and treatment preparation prepared by adopting the bacillus methylotrophicus can be used for preventing and treating the plant diseases caused by botryis cinerea.

Description

technical field [0001] The invention relates to the technical field of biological control of plant diseases, in particular to a methylotrophic bacillus and its biological control preparation and application. Background technique [0002] Botrytis cinerea is a weakly parasitic phytopathogenic fungus, which can infect more than 200 kinds of plants, not only in the growth period, but also in the storage period. Botrytis cinerea has the characteristics of rapid reproduction, wide host range, and strong pathogenicity, and has gradually become the only disease in vegetable production in protected areas. Botrytis cinerea mainly causes root rot, blight, rot of plant seedlings and cuttings, and necrosis of stems. Plants infected with Botrytis cinerea before fruit ripening will cause the fruit tissue to soften and rot, which will seriously affect fruit growth and damage agricultural production. Infection of Botrytis cinerea during storage and transportation after fruit ripening will...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00C12R1/07
CPCA01N63/00A01N63/10C12N1/20C12N1/205C12R2001/07
Inventor 雍道敬牛赡光
Owner 青岛中达农业生物科技有限公司
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