1580 results about "Paenibacillus species" patented technology

The subject invention relates to the surprising discovery that toxin complex (TC) proteins, obtainable from Xenorhabdus, Photorhabdus, and Paenibacillus, can be used interchangeably with each other. In particularly preferred embodiments of the subject invention, the toxicity of a “stand-alone” TC protein (from Photorhabdus, Xenorhabdus, or Paenibacillus, for example) is enhanced by one or more TC protein “potentiators” derived from a source organism of a different genus from which the toxin was derived. As one skilled in the art will recognize with the benefit of this disclosure, this has broad implications and expands the range of utility that individual types of TC proteins will now be recognized to have. Among the most important advantages is that one skilled in the art will now be able to use a single set of potentiators to enhance the activity of a stand-alone Xenorhabdus protein toxin as well as a stand-alone Photorhabdus protein toxin. (As one skilled in the art knows, Xenorhabdus toxin proteins tend to be more desirable for controlling lepidopterans while Photorhabdus toxin proteins tend to be more desirable for controlling coleopterans.) This reduces the number of genes, and transformation events, needed to be expressed by a transgenic plant to achieve effective control of a wider spectrum of target pests. Certain preferred combinations of heterologous TC proteins are also disclosed herein. Other objects, advantages, and features of the subject invention will be apparent to one skilled in the art having the benefit of the subject disclosure.

The present invention relates to a novel strain of Bacillus sp. D747 (deposited as FERM BP-8234) and methods for controlling plant diseases and insect pests, comprising administering cultures of Bacillus sp. D747 (including the viable bacteria) or viable bacteria isolated by culturing, on the plant parts such as roots, stems, leaves, seeds, and the like, or in the culture soil.

The invention relates to a bacillus amyloliquefaciens growing in a disease-preventing and growth-promoting plant and application thereof, relating to bacillus amyloliquefaciens and the application thereof. The bacillus amyloliquefaciens TF28 growing in the disease-preventing and growth-promoting plant belongs to bacillus, has the storage number of CGMCC No.4038 and the storage data of July 26, 2010. The application of the bacillus amyloliquefaciens TF28 comprises the following steps of: (1) preparing activated bacilli liquid; (2) inoculating the activated bacilli liquid in an NYD culture fluid to obtain a strain fermentation solution; and (3) soaking seeds in a dilution solution 50 times of the strain fermentation solution. The strain TF28 in the invention can be colonized, reproduced and transmitted in plants, can simultaneously generate two antibacterial substances, i.e. antimicrobial proteins and lipopeptide antibiotic and has the multiple efficacy of preventing diseases, stimulating growth, increasing the yield, improving the quality, widening an antibacterial spectrum, and the like. Rice seeds treated by the dilution solution 50 times of the fermentation solution of the strain TF28 reach a room-temperature bakana resistance rate over 84.6 percent.

The invention relates to plant endogenous bacillus velezensis which is capable of preventing and treating plant diseases and insects and has functions of growth promotion and drought resistance, and application thereof. The preservation number of the Bacillus velezensis E6 provided by the invention in the China General Microbiological Culture Collection Center is CGMCC No. 9665. The strain is separated from a plant root, is rapid to grow, can be rapidly and greatly colonized at plant roots and has ecological niche advantages; the strain E6 not only has an efficient prevention and treatment function on plant soil-borne fungal diseases and is capable of promoting growth of plant root systems and improving drought resistance of plants, but also is capable of damaging formation of pathogenic bacterium bacterial biofilms, is free of drug resistance of bacteria, has relatively good advantages when being compared with other fungicides, is simple in nutrition requirement and low in productioncost, and has good application prospects.

The invention discloses bio-organic fertilizer, and belongs to the technical field of organic fertilizer. The proportion by weight of all raw materials of the bio-organic fertilizer is dried chicken manure : edible mushroom : compound microbial agent : corn flour or rice bran : functional strain = 110-140 : 390-360 : 0.1-0.5 ; 0.1. The compound microbial agent comprises mould, bacillus, lactic acid bacteria, actinomycetes and saccharomycetes. The strains are efficient viable bacteria. Each gram of the compound microbial agent comprises the number of the mould ranges from 5 hundred million to 8 hundred million, the number of the efficient viable bacteria of bacillus ranges from 12 hundred million to 15 hundred million, and the number of the actinomycetes ranges from 10 hundred million to 12 hundred million and the saccharomycetes of 15 hundred million to 18 million. According to the bio-organic fertilizer, the chicken manure and mushroom dregs serve as main materials, and the high-quality organic fertilizer can be fermented quickly by adding the compound microbial agent and the functional strain.

The invention discloses a denitrifying strain with nitrifying function, a strain-containing water body improver of multiple active microorganisms and a preparation method of the water body improver, belonging to the technical fields of aquaculture and water body improvement. The strain disclosed by the invention is conserved in the China center for type culture collection (CCTCC), the CCTCC NO isM2011443; and the water body improver of the active microorganisms which is based on the strain comprises multiple strains such as Bacillus subtilis, Bacillus licheniformis, Bacillus pumilus, Bacillus cereus, Bacillus megaterium, Rhodopeudomonas palustris, Arthrobacter globiformis, Rhodotorula rubra and Lactobacillus plantarum. The strain is used to perform liquid fermentation and obtain fermentation liquor of which number of live bacteria is 0.5-3*10<10>cfu / mL, bacterial powder of which the number of live bacteria is 0.2-2*10<12>cfu / mL is further prepared; and the bacterial powder is used along with a diluent to prepare a liquid or powdery water body improver, wherein the effective live bacteria of the water body improver is 0.1-3*10<10>cfu / mL(or cfu / g). The water body improver has the function of reducing the contents of ammonia nitrogen, nitrites and nitrates in the culture water body; and the water body improver is suitable for different culture water bodies and has the function of improving the water quality.

The invention discloses a bacillus megaterium strain X3 and application thereof to plant growth promotion. The bacillus megaterium strain X3 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on May 11, 2015 with the preservation number of CGMCC No.10803, is obtained through separation of indoleacetic acid, has the characteristics of siderophore production, phosphate solubilizing, ammonia producing, salt tolerance and disease suppression, can remarkably promote crop growth and improve corn yield, and meanwhile, can increase the diversity and the abundance of microbes in soil. The bacillus megaterium strain X3 is quick in reproduction, simple in production process, strong in adversity ability, easy to preserve and conductive to industrial production, and can not only prevent and cure soil-borne diseases but also promote plant growth when utilized to prepare a bio-fertilizer, thereby having a wide application prospect.

The invention discloses a Bacillus velezensis CGMCC (China General Microbiological Culture Collection Center) No.4992 and application thereof in tomato blight antagonism bacteria. A farmland soil sample on which tomatoes are planted for years in Xinjiang Fukang is subjected to antagonism bacteria screening to obtain a strain with stronger bacteriostatic activity; the strain is further screened and domesticated and bred; and the obtained strain is subjected to morphological specificity and 16S rRNA (Ribose Nucleic Acid) sequencing and system development analysis. The antagonism of the fermentation broth of the strain has outstanding technical effect on solving the tomato blight antagonism bacteria and has wide application field.

The invention discloses bacillus amyloliquefaciens and application thereof in preventing and controlling peach bleeding diseases. The collection number of the Bacillus amyloliquefaciens H47 is CCTCC NO: M2013283. A biological agent which resists the peach bleeding diseases is prepared by carrying out liquid fermentation on the strain can be applied to peach trees; the liquid fermentation process comprises the following steps of: slant strain activation, seed culture and liquid deep fermentation culture. The strain disclosed by the invention and thallus-free spore-free fermentation liquor can both generate a higher antagonistic effect on the pathogen botryosphaeria of the peach bleeding diseases; the biological agent disclosed by the invention achieves an outstanding preventing and controlling effect on peach tree bleeding diseases; compared with the traditional chemical agent, the biological agent disclosed by the invention has the advantages of greenness, safety, environment friendliness, and the like and has wide application prospect on the aspect of biological prevention and control of the bleeding diseases.

The invention discloses a bacillus velezensis strain JN5 and an application thereof. The bacillus velezensis strain JN5 is registered in CGMCC on 13th, December, 2016, the preservation number being CGMCC No.13455. The bacillus velezensis strain JN5 provided by the invention can be prepared into an aqueous agent or a coating agent to be acted on crops such as corn and rice for preventing bacterial stripe of rice and bacterial blight of rice and promoting growth of corn and rice. The strain is safe to use and free of residues, the dosage and residual amount of a chemical agent in an agricultural product are favorably reduced, the production cost is lowered, and the strain is high in practicality and suitable for industrial production.

The microbial fertilizer is mixture of fermented liquids separately with photosynthetic bacteria, bacillus, actinomycete and lactic acid bacteria. It is prepared through inoculating photosynthetic bacteria, bacillus, actinomycete and lactic acid bacteria separately to the first stage culture medium, and culturing to obtain the first stage bacterial seeds; inoculating the first stage bacterial seeds separately to second stage culture medium, and culturing to obtain the second stage bacterial seeds; and inoculating the second stage bacterial seeds separately to the fermenting culture medium, and culturing to obtain fermented bacterial liquids; and mixing the fermented bacterial liquids in certain proportion to obtain the microbial fertilizer. The present invention also relates to the application of the microbial fertilizer in controlling diseases of tobacco and trees and the usage.

The present invention discloses a preparation method and application of Bacillus coagulans and a spore preparation cultured in high density, and belongs to the microbe technical field. A Bacillus coagulans strain is classified and named Bacillus coagulans JSSW-LA-07 and is preserved as CGMCC No.2602 in China General Microbiological Culture Collection Center. The Bacillus coagulans strain JSSW-LA-07 can produce spore under an aerobic condition and produce a small amount of L-lactic acid in the metabolism during a course of fermentation. The Bacillus coagulans strain can produce L-lactic acid with a higher purity under an anaerobic condition. The strain does not produce substances which can be harmful to human and cultured animals, has the characteristics of innocuity, on residue, no side effect and the like, has a great significance for production of green and safe livestock, fowls and marine products and improvement of cultivation ecological environment, and can be widely applied in the cultivation production of high yield, high quality, high efficiency, synusiologic and safe livestock, fowls and marine products, and the regulation and control of the ecological environment of cultivation.

The invention discloses bacillus megaterium and an application thereof, and belongs to the field of microorganisms. A plant growth promoting bacterium (bacillus megaterium JX15) is collected in the China General Microbiological Culture Collection Center on Dec.20, 2011, the class name is bacillus megaterium and the collection number is CGMCC No.5622. The bacillus megaterium has the beneficial effects that a strain can produce indoleacetic acid with high yield, can be used for nitrogen fixation and can grow by using sparingly-soluble phosphates as phosphorous sources, so as to improve the utilization rates of fertilizers, promote plant root development and absorption of the fertilizers, and increases the contents of soil mineral nitrogen and available phosphorus; the strain has a good effect on promoting growth of peanuts; the high-yield indoleacetic acid promotes the growth and the development of the peanuts; and the nitrogen and phosphate fertilizer utilization rates of the peanuts are relatively high due to the increasing of the contents of the soil mineral nitrogen and the available phosphorus.

The invention belongs to the technical field of agricultural biocontrol, and especially relates to a broad-spectrum disease resistance bacillus and an application thereof. The biocontrol bacillus is classified and named as Bacillus velezensis, and the preservation number is CGMCC. No.15766. The Bacillus velezensis has the advantages of broad antibacterial spectrum, strong antagonistic effect, longlasting period, and effectiveness in inhibition of the growth of crop pathogenic fungi, and can be used in the fields of agricultural biocontrol to prevent and control crop diseases caused by gray mold, blight, anthracnose, stem rot, seedling blight, stalk break, gibberellic disease and other fungi. The Bacillus velezensis can also be used for promoting the growth of crop seeds and seedlings andthe preservation and fresh-keeping of fruits and vegetables. The Bacillus velezensis has the characteristics of strong resistance to ultraviolet and drought, simple preparation method and low cost, isa biocontrol strain with excellent performance, and has good development and application prospects.

The invention discloses bacillus velezensis ZF2 and application thereof to control of plant diseases, and provides the Bacillus velezensis (Bacillus velezensis ) ZF2 with the preservation number of CGMCC NO.16013. The invention further relates to the bacillus, or a culture solution or bacillus suspension thereof or application of fermentation products of the bacillus to restrain bacteria. The bacterial strain ZF2 is simple in culture condition in the fermentation process, and massive active substances are generated, are secreted outside cells, and have remarkable antagonism effects on 6 disease fungi and 7 pathogenic bacteria. During pot culture test in green houses, the prevention effect of the bacterial strain ZF2 on corynespora cassiicola is as high as 92.24%, test proves that the bacillus velezensis ZF2 has good application prospects and also provides a certain theoretical basis and technical support for development and application of microbial inoculum.

The invention relates to a bio-organic fertilizer and a method for preparing the bio-organic fertilizer which prevents and treatments soil insects. The bio-organic fertilizer is added with active bacteria and other nutrition bacterial genus, wherein the active bacteria and other nutrition bacterial genuses can prevent and treat soil insects. The bio-organic fertilizer comprises biological agents and organic fertilizers, wherein the microbial agents comprise bacterial colonies of Japanese beetle bacilli, withered grass bacilli, enormous bacilli, jelly-formed bacilli, brown ball nitrogen-fixing bacteria and bacillus licheniformis. The Japanese beetle bacilli are the main type of bacterium which prevents and treats soil insects and other types of bacteria are mainly used for fixing nitrogen, decomposing phosphorus, decomposing potassium, and preventing and treating soil-borne diseases. The organic fertilizer comprises livestock and poultry manure and edible mushroom dregs. The livestock and poultry manure and the edible mushroom dregs are mixed, ferment and are thoroughly decomposed, the microbial agents are added and the bio-organic fertilizer is prepared. The bio-organic fertilizer and the method for preparing the bio-organic fertilizer which prevents and treatments soil insects can effectively kill larvas of soil insects. Bacterial colonies of microorganisms grow by fixing nitrogen, decomposing phosphorus and decomposing potassium, the structure of soil is improved, the organic content of soil is improved, the quality of crops is improved and the crop yield is improved.

The invention discloses a farm fertilizer produced by biologic technology, in particular a decomposing agent for rapidly decomposing straws at low cost, the mixture ratio of which is aerobic bacillus subtilis Cohn 20%, Bacillus licheniformis 10%, aspergillus flavus 20%, chaetomiaceae 20%, absidia corymbifera 20%, and saccharomycetes 10%. The production procedures are: extracting different beneficial decomposed bacteria from organics from different environments, purifying, rejuvenating, breeding and culturing; inoculating the cultured bacterial strains into disinfected solid culture medium; fermenting for 4-5 days under 25-50 DEG C, airing dry so that the residual moisture in the bacterial strains is 25%; mixing, agitating evenly and packing the aired solid bacterial strains into solid decomposing agent. As the invention is made by using a unique optimization and combination of aerobic bacterial group and a high and new technology and production process, the invention is a pure biologic product, is free from smell and harm for making organic fertilizer by effectively decomposing wastes in the countryside, and the fermenting speed is high, the cost is low, and the source of raw material is wide. The invention has improved the soil structure and reduces environmental pollution.

The invention relates to broad-spectrum compound microbial bacteria for preventing and treating vegetable fungal diseases, and belongs to the technical field of agricultural biological prevention and treatment. The broad-spectrum compound microbial bacteria is compounded from four microorganisms, namely bacillus subtilis BSD-2 ((i) Bacillus subtilis), brevibacillus brevis ch2 ((i) Brevibacillus brevis), bacillus amyloliquefaciens Y10 ((i) bacillus amyloliquefaciens) and paenibacillus polymyxa Y89 ((i) Paenibacillus polymyxa) according to a certain ratio. By adopting the compound bacteria, the vegetable fungal diseases of a greenhouse or a large field, for example, botrytis, leaf mold, downy mildew, powdery mildew, blight and the like can be effectively prevented and treated, the field efficacy can be up to over 70%, the yield is increased by 5-20%, and the broad-spectrum compound microbial bacteria are significant in economic, social and ecological benefits, and have broad popularization and application prospects.

The invention discloses a Paenibacillus kribbensis, and a preparation method and an application thereof. The Paenibacillus kribbensis has a bacterial code name of TRCC82001, is preserved in China General Microbiological Culture Collection Center, and has a preservation number of CGMCCNo.7996. The above bacterial strain has the advantages of good production property, good metabolite antimicrobial effect, and strong activity against rice blast and sheath blight, and a solution obtained after diluting a broth subjected to antibiotic treatment to 100 times has an inhibition effect of above 80% on the rice blast and sheath blight. The bacterial strain and its metabolites have a broad antimicrobial spectrum, can be used for preparing biocontrol preparations for the broad spectrum activity inhibition of pathogenic fungi to control the fungal diseases of plants.

The invention discloses an endophyte which is classified and named Bacillus megaterium and is completely named Bacillus megaterium SAN1. The endophyte is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March. 11, 2013 and has a preservation number of CGMCC No.7295. The invention also discloses an application of the endophyte in repair of facility soil secondary salinization and Cd combined pollution. The Bacillus megaterium CGMCC 7295 disclosed by the invention can be used for prompting growth of plants, improving physical and chemical properties of soil, promoting the plants to absorb Cd, effectively eliminating Cd pollution in the soil, efficiently converting nitrate nitrogen of soil and alleviating the hazards of secondary salinization; and due to combined action between the Bacillus megaterium CGMCC 7295 and the plants, the facility soil secondary salinization and the heavy metal Cd combined pollution can be repaired.

The invention discloses a biological fertilizer, comprising, by weight, 65-80 parts of a concentrated nmolasses fermentation broth, 7-14 parts of potassium humate, 0.5-2 parts of a chelated microelement, 1-5 parts of microbial strains and 5-10 parts of a surfactant, wherein the chelated microelement is a mixture of EDTA-Cu, EDTA-Fe, EDTA-Mn and EDTA-Zn; and the microbial strains are composed of paenibacillus polymyxa, bacillus subtilis and bacillus mucilaginosus. The biological fertilizer is an environment-friendly type fertilizer, can improve soil environment and increase crop output, can be widely applied in plants particularly crops, has a very significant yield-increasing effect, and can increase the output of food crops, vegetables, melons and fruits by 10%-20%.

The invention discloses a strain of efficient bacillus amyloliquefaciens, and a bacterial agent and application thereof, wherein the Latin name of the bacillus amyloliquefaciens is Bacillus amyloliquefaciens; the strain number is HW05; the accession number of the bacillus amyloliquefaciens in China General Microbiological Culture Collection Center is CGMCC No. 10273. The efficient bacillus amyloliquefaciens provided by the invention has the obvious inhibition effects on fusarium oxysporum, phytophthora capsici, ralstonia solanacearum, pyricularia grisea, colletotrichum gloeosporioides and root-knot nematode. Through the single application of HW05, the efficient prevention and control effects can be achieved on leaf surface disease sweet melon powdery mildew, mango anthracnose diseases andmulberry red rust diseases; the HW05 and paecilomyces lilacinus can be matched for preventing and treating soil-borne banana wilting diseases and banana root-knot nematode diseases, and a good synergyeffect is achieved.

The present invention provides Bacillus velezensis for control of cucumber fusarium wilt and application thereof. The Bacillus velezensis is coded as FJ17-4, and the registration number is CGMCC No. 14642 in the China General Microbiological Culture Collection Center. The Bacillus velezensis FJ17-4 has a significant antagonism effect on cucumber fusarium wilt-Fusarium oxysporum f.sp cucumerinum, and can effectively control cucumber fusarium wilt, and the control effect is stable. The Bacillus velezensis provides an excellent bacterial strain resource for biological control of the cucumber fusarium wilt.

The present invention relates to a kind of Bucillus subtilis strain, belongs to the field of microbiological technology. The Bucillus subtilis strain, named as Bucillus subtilis B9601-Y2, is preserved in the preservation number of CGMCC No. 0954. Bucillus subtilis B9601-Y of the present invention may be used in preventing various plant diseases and pollution to edible fungus, may be used in promoting plant growth, controlling soil transmitted plant pathogeny and preserving fresh cut China rose, and has long shelf period, high resistance and no environmental pollution.

The invention relates to a method for preparing a microbial fertilizer, a nutrient solution, a fodder and a pesticide and for fermentation bed cultivation by utilizing indigenous microorganism probiotics. The microorganism probiotics are formed by fermenting eight pure natural materials, including indigenous bacteria, green juice, fresh fish amino acid, enzyme alcohol, Chinese prescription nutrient solution, lactic acid bacteria, natural calcium and water-soluble calcium phosphate potassium; the microorganism probiotics contain the mixed flock of various beneficial bacteria, such as, lactic acid bacteria, photosynthetic bacteria, bacillus and yeast; the fertilizer can be used for fertilizing and loosening soil; the agricultural products are free from residue, taste nice and are high in yield; the fodder can be used for guaranteeing no residue on the cultured animal meat product; the meat is tender and free from smell; after the microorganism nutrient solution is used, the microorganism pesticide can prevent and treat insect diseases; the chemical pesticide and the fertilizer residue can be eliminated and decomposed; when the fermentation bed is used for culturing, the excrement cleaning is not required; the animal excrement is decomposed by the fermented padding while beneficial mycoprotein is formed; the fodder can be eaten by the animals; the microbial fertilizer is odorless, zero-emission, environment-friendly, pollution-free to environment and beneficial to the health of human and animals.

The invention discloses a biological salt-degrading bacterial fertilizer consisting of the following components by weight proportion: 0.0013 to 0.0045 portions of gibberellin, 0.00002 to 0.0002 portions of cytokinins, 15 to 35 portions of bacillus megaterium, 50 to 80 portions of salt-degrading flora, 5 to 15 portions of amino acid and 2 to 10 portions of humic acid. The materials are evenly mixed and air dried. The bacterial fertilizer is formed by being extracted from marine microorganisms, rejuvenation, expanding propagation and being remixed with a plurality of trace elements. The bacterial fertilizer is scientific in formulation and very strong in activity, can be widely applied to salt lick improvement and a secondary salinization treatment, improves and optimizes soil environment and improves the deteriorated soil environment caused by the long-term use of chemical fertilizers and salt containing organic fertilizers (dungs). The bacterial fertilizer can facilitate the development of the roots of crops and can improve the ability of absorbing night soil. By applying the biological salt-degrading bacterial fertilizer, the fertilizer can facilitate the even seedling, uniform seedling and strong seedling at the earlier stage of crops, the strong nutrient transmission capacity at the middle stage and no premature senescence at the later stage.

The invention discloses a series of high-efficiency producing strains of sauce-flavor characteristic aroma and its application in Chinese liquor brewing, belonging to the technical field of bioengineering. The bacterial strain provided by the present invention, which is classified as Bacillus licheniformis, is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation numbers are CGMCC NO: 3961, CGMCC NO: 3962, and CGMCC NO: 3963. The strains are derived from high-temperature Daqu, a Chinese liquor, and can grow at a high temperature of 55°C. Fermented in liquid and solid media made of bran, barley, wheat, and sorghum, they can all produce an obvious characteristic aroma of sauce, and both It can be fermented and metabolized to produce C4, pyrazines, acids, phenols, and aromatic rings. The strains are used alone or in combination in the production process of sauce-flavored and sesame-flavored liquors. The quality of the produced liquors is improved, and the characteristic aroma is more prominent and longer-lasting. concentration increased.

The invention discloses a microbial decomposition agent for animal wastes and straws and a preparation method thereof. The microbial decomposition agent comprises the following strains in parts by weight: bacillus, saccharomycetes, Aspergillus niger, Aspergillus oryzae, trichoderma sp, lactic acid bacteria, photosynthetic bacteria and actinomycetes. The decomposition agent has diversified functions, can be simultaneously used for composting straws and / or animal wastes, contains multiple functional bacteria and is high in microbe activity, the selected strains achieve a synergistic effect, do not generate antagonism, and the decomposition agent has the advantages of fast temperature rise and high temperature of fermented materials, complete decomposition, short decomposition time and the like. The preparation method of the microbial decomposition agent disclosed by the invention has the advantages of simplicity, feasibility, simple operation and low production cost.

The invention discloses bacillus pumilus and application thereof, which belong to the technical field of microbes. The produced strain of the invention is named Bacillus pumilus Van35 according to classification, and the preservation number of the strain is CGMCC No.3411. When the strain preparation is spayed, the soluble total sugar content and the reducing sugar content of tobaccos can be increased, while protein, total nitrogen and nicotine content are reduced. Therefore, specific values of the ratio of dextrose to nitrogen, the ratio of total sugar to betaine and the ratio of total nitrogen to total alkaloid tend to rise, the quality of chemicals is high and the quality of the smoke panel test is improved.