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Broad-spectrum disease resistance bacillus and application thereof

A bacillus and biocontrol technology, which is applied in the field of broad-spectrum disease-resistant biocontrol bacillus, can solve the problem that the biocontrol technology for tomato diseases is immature, cannot provide a variety of pathogenic biocontrol methods, and has a narrow antibacterial spectrum of biocontrol bacteria. and other problems, to achieve good development and application prospects, reduce decay rate and weight loss, strong UV resistance and drought resistance.

Active Publication Date: 2018-12-18
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the biological control technology for tomato diseases is still immature, and the screened bio-control bacteria have a narrow antibacterial spectrum, which can only achieve bio-control effects against a certain pathogenic bacteria, and cannot provide a broad-spectrum against multiple pathogenic bacteria. biological control methods

Method used

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  • Broad-spectrum disease resistance bacillus and application thereof
  • Broad-spectrum disease resistance bacillus and application thereof
  • Broad-spectrum disease resistance bacillus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A number of strains of bacteria were isolated and purified from the rhizosphere soil of plants by plate dilution coating method and plate streaking method, and Botrytis cinerea was used as indicator bacteria, and screened by double-layer paper ring method to obtain a strain against Botrytis cinerea Bacteria with antagonistic effect are numbered WZ-37. The morphological characteristics, physiological and biochemical characteristics and molecular biological identification of the strain were carried out, and the results are as follows:

[0031] (1) Morphological characteristics

[0032] Observe the vegetative body and spores of WZ-37 under a microscope. The strain belongs to Gram-positive bacteria. figure 1 ; Inoculate the strain on LB solid medium for streak culture, culture at 35°C for 24 hours, the colony is off-white, round, with wet surface, wrinkled, opaque, and neat edges, and its colony shape is shown in figure 2 .

[0033] (2) Physiological and biochemical cha...

Embodiment 2

[0041] In this embodiment, the filter paper ring confrontation culture method was adopted, and the Bacillus Velez screened in Example 1 was subjected to a plate confrontation test with various plant pathogenic bacteria to determine the antibacterial spectrum of the Bacillus Velez.

[0042] Plant pathogens tested include: Botrytis cinerea, Tomato wilt, Cucumber wilt, Cucumber anthracnose, Corn stalk rot, Fusarium equisetum, Verticillium fusarium, Solanum blight, Watermelon wilt, Melon wilt, Chain Glycyrrhizae, Sclerotinia sunflower, Fusarium graminearum F609 and Fusarium graminearum F1403;

[0043] The specific test method is as follows:

[0044] Filter paper ring confrontation culture method: pour the sterilized PDA medium onto the plate, place the circular bacterial mass of the phytopathogen to be tested with a diameter of 5 mm on the center of the PDA plate after cooling, and place a sterile filter paper ring at a distance of 3 cm from the center. On the filter paper ring, ...

Embodiment 3

[0049] This embodiment tested the inhibitory effect of the Bacillus velei screened in Example 1 on the mycelial growth of plant pathogenic fungi. Bacteria-WXCDD105, this strain belongs to Bacillus subtilis subsp.subtilis, the specific test method is:

[0050] According to the inoculum amount of 1%, the culture solution of Bacillus Veles and the control bacterium WXCDD105 cultivated at 35°C and 180r / min for 24 hours were respectively inserted into 50mL PD culture solution, and then a 5mm diameter tomato cinerea cake was added. The blank group was only inoculated with Botrytis cinerea without biocontrol bacteria, cultured at 28°C and 120r / min for 7 days, filtered with sterile dry filter paper, and the mycelia of Botrytis cinerea collected were dried and weighed. The same method was used to detect the inhibitory effect of Bacillus Velez and control bacteria WXCDD105 on tomato fusarium wilt, cucumber anthracnose and cucumber fusarium wilt hyphae growth, the test results are shown ...

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Abstract

The invention belongs to the technical field of agricultural biocontrol, and especially relates to a broad-spectrum disease resistance bacillus and an application thereof. The biocontrol bacillus is classified and named as Bacillus velezensis, and the preservation number is CGMCC. No.15766. The Bacillus velezensis has the advantages of broad antibacterial spectrum, strong antagonistic effect, longlasting period, and effectiveness in inhibition of the growth of crop pathogenic fungi, and can be used in the fields of agricultural biocontrol to prevent and control crop diseases caused by gray mold, blight, anthracnose, stem rot, seedling blight, stalk break, gibberellic disease and other fungi. The Bacillus velezensis can also be used for promoting the growth of crop seeds and seedlings andthe preservation and fresh-keeping of fruits and vegetables. The Bacillus velezensis has the characteristics of strong resistance to ultraviolet and drought, simple preparation method and low cost, isa biocontrol strain with excellent performance, and has good development and application prospects.

Description

technical field [0001] The invention belongs to the technical field of agricultural biological control, and in particular relates to a broad-spectrum disease-resistant biocontrol bacillus and its application. Background technique [0002] Tomato gray mold and tomato wilt are the main diseases that occur during tomato planting and cultivation. They have a high incidence rate and fast transmission speed, and may occur throughout the entire growth cycle of tomatoes; And it will cause the quality of tomato fruit to decline. At present, chemical control is still the main method to prevent and control tomato gray mold and tomato wilt. Although chemicals have played an extremely important role, their disadvantages are becoming more and more obvious. Long-term extensive use of chemical agents will increase the resistance of pathogens, reduce the effect of disease control, and cause serious soil and environmental pollution, endangering the health of humans and animals, and even des...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P21/00A01C1/00A23B7/155A01G13/00C12R1/07
CPCA01C1/00A01G13/00A01N63/00A23B7/155A23V2002/00C12N1/20C12N1/205C12R2001/07A23V2200/10
Inventor 王傲雪黄海锋郭兰陈秀玲刘佳音张瑶张棚
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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