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Bacillus subtilis strain

A technology of Bacillus subtilis and strains, applied in the field of microorganisms, can solve problems such as pollution, ineffective effects, and edible fungus biocontrol agents that have not yet been seen

Inactive Publication Date: 2004-12-15
何月秋
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Problems solved by technology

Although many biocontrol agents have been proven to have control effects, only a few are used in production, and most of them are still in the research and exploration stage
However, the biocontrol agents used in production generally can only prevent a few types of diseases, and do not have broad-spectrum control capabilities, and the effect of promoting plant growth is not obvious
Edible fungus is a major industry in my country, but the culture material of edible fungi is seriously polluted by filamentous fungi such as Trichoderma in production. In order to prevent pollution, the production mainly relies on high-temperature sterilization of the culture material and adding fungicides when preparing the material. This has not only increased the cost, but also may contain fungicides in edible fungus products, so it is necessary to find a safer, more effective and economical method
However, there are no reports of biocontrol agents for edible fungi

Method used

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  • Bacillus subtilis strain

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Embodiment Construction

[0013] Bacillus subtilis B9601-Y2 was isolated from the wheat field soil in Qingzhou City, Shandong Province. Two kinds of liquid culture medium were used for the cultivation of the strain: (1), PS medium: fresh potato 200g decocted juice, sucrose 15g, and water 1000ml for sterilization; (2), LB medium: tryptone 10g, yeast extract 5g, sodium chloride 10g, water 1000m, sterilized for later use. According to the different culture medium, there are two ways to cultivate the biocontrol strains: (1), liquid culture: inoculate the biocontrol bacteria in LB or PS medium, and cultivate them on a shaking table with a rotation speed of 120r / m at a temperature of 35°C 2 to 3 days; (2), solid culture: inoculate in PSA medium (decoction of 200g of fresh potatoes, 15g of sucrose, 17g of agar, add 1000ml of water to sterilize), and cultivate in a 37°C incubator for 3 to 4 days. The preparation method of the bacterium solution is to dilute the bacterium solution cultivated in liquid to the re...

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Abstract

The present invention relates to a kind of Bucillus subtilis strain, belongs to the field of microbiological technology. The Bucillus subtilis strain, named as Bucillus subtilis B9601-Y2, is preserved in the preservation number of CGMCC No. 0954. Bucillus subtilis B9601-Y of the present invention may be used in preventing various plant diseases and pollution to edible fungus, may be used in promoting plant growth, controlling soil transmitted plant pathogeny and preserving fresh cut China rose, and has long shelf period, high resistance and no environmental pollution.

Description

technical field [0001] The invention belongs to the technical field of microbes, and mainly relates to a bacterial strain capable of broad-spectrum preventing and controlling different types of plant diseases and edible fungus pollution and promoting plant growth. Background technique [0002] Chemical pesticides are an important resource for the prevention and control of plant diseases, but due to excessive and wrong use, there are risks of pesticide residues, environmental pollution and ecosystem damage. Therefore, it is urgent to find an alternative, safer and more effective control method. Although many biocontrol agents have been proven to have control effects, only a few are used in production, and most are still in the research and exploration stage. The biocontrol agents that have been used in production generally can only prevent a few types of diseases, and do not have broad-spectrum control capabilities, and the effect of promoting plant growth is not obvious. E...

Claims

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Application Information

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IPC IPC(8): C12N1/20
Inventor 何月秋马平
Owner 何月秋
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