2800 results about "Microbiological Techniques" patented technology

The invention discloses a compound microorganism liquid bacterial fertilizer for preventing plant diseases and insect pests and a preparation method of the compound microorganism liquid bacterial fertilizer and belongs to the technical field of microorganisms. The bacterial fertilizer comprises raw materials of, by weight, 10-20 parts of paprika powders, 10-20 parts of chilli powders, 5-15 parts of ginkgo leaf powders, 5-15 parts of chinaberry powders, 5-15 parts of lightyellow sophora root powders, 20-40 parts of mashed garlic, 40-80 parts of molasses, 4-8 parts of soybean meal, 5-15 parts of calcium sulfate, 5-15 parts of magnesium chloride, 5-10 parts of salt, 5-15 parts of photosynthetic bacterium strains, 5-15 parts of saccharomycete strains, 5-15 parts of lactic acid bacterium strains, 5-15 parts of streptomyces jingyangensis strains, 5-15 parts of bacillus subtilis strains and 800 parts of water. According to the compound microorganism liquid bacterial fertilizer, probiotic floras which are screened and are capable of coexisting are used for fermenting plant leaching agents of pepper, chilli, ginkgo leaves, chinaberry, lightyellow sophora roots and garlic, the plant leaching agents are capable of expelling and killing the insect pests and harmful bacteria, the compound microorganism liquid bacterial fertilizer has the advantages of being high in content of live bacteria of probiotics, high in activities, high in stability, small in using amount of plant materials, fast in acting effects, non-toxic and environment-friendly, and has wide application prospects in agricultural planting.

The invention discloses bifidobacterium bifidum capable of remarkably relieving constipation and application thereof and belongs to the technical field of microorganisms. The bifidobacterium bifidum CGMCC NO.13632 can well tolerate simulative gastrointestinal fluid, can be well attached to colon cancer cells HT-29, and can remarkably increase the water content of excrement of constipation mice and shorten the first tarry stool time. On the aspect of increasing the small intestine driving rate, the bifidobacterium bifidum CGMCC NO.13632 is superior to cathartic phenolphthalein, a best effect is shown, secretion of gastrointestinal regulatory peptide relevant to constipation in serum is also adjusted while constipation pathological factors are remarkably improved, and the bifidobacterium bifidum does not have toxic and side effects of medicine for treating constipation, and is a first choice for preventing and treating constipation. The bifidobacterium bifidum CGMCC NO.13632 is used for preparing pharmaceutical compositions and fermented food for relieving constipation, and has very wide application prospects.

The invention relates to a microbe soil repairing additive and process for preparation. The process for preparation comprises that liquid microbe fertilizer containing photosynthetic bacteria, bacillus, actinomycete and lactic acid bacteria are absorbed to one or a plurality of natural organic fertilizers as grass peat and zeolite powder or the like, to be mixed proportionally. The invention further relates to the application of the microbe soil repairing additive for repairing soil. The invention can lead useful microbe into soil, uses microbe technique to transfer, absorb, degrade, and convert the contaminant in soil, adjust soil pH value, eliminate soil blocking, and restrain soil-borne disease, thereby improving soil structure, to return soil to original ecologic structure. And the viable bacteria group of the invention is planted in plant root, to quickly grow and deeply insert plant root, thereby improving yield, quality and level of agricultural product, as one ideal fertilizer of organic agriculture, with wide application in agriculture continuous development.

The present invention discloses a preparation method and application of Bacillus coagulans and a spore preparation cultured in high density, and belongs to the microbe technical field. A Bacillus coagulans strain is classified and named Bacillus coagulans JSSW-LA-07 and is preserved as CGMCC No.2602 in China General Microbiological Culture Collection Center. The Bacillus coagulans strain JSSW-LA-07 can produce spore under an aerobic condition and produce a small amount of L-lactic acid in the metabolism during a course of fermentation. The Bacillus coagulans strain can produce L-lactic acid with a higher purity under an anaerobic condition. The strain does not produce substances which can be harmful to human and cultured animals, has the characteristics of innocuity, on residue, no side effect and the like, has a great significance for production of green and safe livestock, fowls and marine products and improvement of cultivation ecological environment, and can be widely applied in the cultivation production of high yield, high quality, high efficiency, synusiologic and safe livestock, fowls and marine products, and the regulation and control of the ecological environment of cultivation.

The present invention relates to a method of using spray drying for preparing probiotics micro-capsules, belonging to the technical field of micro-organisms. According to the method, a probiotic suspension or probiotics adsorbed in porous starch is used as a core material, gum arabic and maltodextrin are used as a wall material, and the probiotics micro-capsules are prepared through a spray drying process. The method comprises the steps of: arabic gum and maltodextrin at a ratio of 1:7 to 1:10 as the wall material, at a concentration of 15 to 20% dissolved in deionized water, mixing uniformly with the probiotic suspension or the probiotics adsorbed in porous starch at room temperature; and spray drying under conditions of a inlet air temperature being 130-150DEG C and a feeding rate being 12.50-15.50mL / min, wherein skim milk powder is adopted as a heat-resistant protective agent in an amount of 1 to 3%, and embedding rate of the probiotics is above 85% by using the method of the invention. The method has the advantages of being fast in drying rate, short in time, low in material temperature, good in product dispersion and solubility, simple in production process, stable in effect, long in shelf life of products, and suitable for continuous production.

A method for producing L-lactic acid by Bacillus coagulans CGMCC No.2602 belongs to the technical field of microorganisms. In the invention, Bacillus coagulans CGMCC No.2602 is adopted, and under the condition of no oxygen supply, starchiness hydrolyzed sugar or dextrose is fermented by a semicontinuous intermittent fermentation way or an inter-sugar-compensating fermentation way to generate L-lactic acid with high optical purity. The invention has the advantages that the gemma property of the Bacillus coagulans is stable, and the L-lactic acid obtained by inoculating and fermenting the starchiness hydrolyzed sugar has high optical purity and rate of conversion of sugar and acid and short fermentation period; in addition, the semicontinuous intermittent fermentation way saves the time for preparing seeds by a continuous reladling and subculturing method, shortens the fermentation period, enhances the fermentation strength and obtains the L-lactic acid with both relatively high rate of conversion of sugar and acid and purity.

The invention discloses bifidobacterium longum and applications thereof, and belongs to the technical field of microorganisms. The bifidobacterium longum is deposited in the China General Microbiological Culture Collection Center in Institute of Microbiology, Chinese Academy of Sciences at number 3, No.1 yard, Beichenxi road, Chaoyang district, Beijing on December 7, 2017, and the accession numberis CGMCC NO.15032. The bifidobacterium longum has good growth, acid production, and acid resistant properties, and high adhesive capacity, can significantly increase the water content of faeces and the pushing rate of small intestine of mice with constipation, shortens the first black stool evacuation time, relieves inflammation of colonic mucosas, and regulates contents of gastrointestinal peptides related to constipation in serum. A constipation relieving effect of the bifidobacterium longum is equivalent to that of bifidobacterium animalis BB12, but an effect of the bifidobacterium longumon the pushing rate of small intestine is superior to those of bifidobacterium animalis BB12 and phenolphthalein. Accordingly, the bifidobacterium longum strain CCFM760 can be widely applied in various foods or medicine matrixes.

A microecological water quality conditioning agent applicable to restoring of sea cucumber culture water body and a method for preparing the microecological water quality conditioning agent belong to the technical field of aquaculture and microorganisms. By aiming at physiological habits and culturing characteristics of sea cucumbers and applying modern biological technique, the microecological water quality conditioning agent is made of bacillus flexus CGMCC (China general microbiological culture collection center) No.5115, bacillus subtilis CGMCC No.5116 and bacillus cereus CGMCC No.5117, which are separated and sieved from a sea cucumber culturing environment and prepared by steps: using corn starch and soybean cake powder as main culturing materials, fermentating for 24-48h at 25-40 DEG C, and using zeolite and wheat bran as adsorption carriers for processing to obtain the microecological water quality conditioning agent. The microecological water quality conditioning agent is less susceptible to environment conditions, has remarkable purifying effect on sea cucumber culturing water body, and is capable of greatly reducing content of harmful substances such as ammonia nitrogen, nitrite, hydrogen sulfide and the like, deodorizing and effectively restraining pathogenic bacteria so as to prevent sea cucumber diseases and improve yield and quality of cultured sea cucumbers.

The invention discloses a manufacturing method of butyrate spindle bacillus microecological agent, which comprises the following steps: preparing culture medium; dissolving the culture medium; adjusting pH value; loading in the ferment tank; heating to sterilize the culture medium in the ferment tank; inoculating the butyrate spindle bacillus seed; controlling temperature to ferment; detecting the bacteria number of ferment liquid; adsorbing ferment liquid through plant fiber powder; drying; grinding; obtaining the powder agent.

The present invention relates to a kind of Bucillus subtilis strain, belongs to the field of microbiological technology. The Bucillus subtilis strain, named as Bucillus subtilis B9601-Y2, is preserved in the preservation number of CGMCC No. 0954. Bucillus subtilis B9601-Y of the present invention may be used in preventing various plant diseases and pollution to edible fungus, may be used in promoting plant growth, controlling soil transmitted plant pathogeny and preserving fresh cut China rose, and has long shelf period, high resistance and no environmental pollution.

The invention belongs to the technical fields of feeds, enzyme preparations and microorganisms, and particularly relates to a preparation method of a feeding-attractive glucolytic molasses feed. The preparation method includes following steps: mixing feed raw materials, feed-use microorganisms, a feed-use composite enzyme, molasses and water according to proportion, performing fermentation enzymolysis to prepare a glucolytic feed wet base material, mixing the glucolytic feed wet base material with molasses to prepare a glucolytic molasses feed wet base material, and finally drying the glucolytic molasses feed wet base material to prepare the feeding-attractive glucolytic molasses feed. The feeding-attractive glucolytic molasses feed include certain amounts of molasses, small peptides, lactic acid, yeast protein and probiotics and such small-molecular nutrients, and is good in palatability and high in digestion rate.

The invention relates to an Acinetobacter calcoaceticus WG-6 capable of degrading oil and a culture method and application thereof, and belongs to the technical field of microorganisms. The bacterial strain has been collected in a China General Microbiological Culture Collection Center on June 11th, 2010 with a collection number of CGMCC No.3915. The Acinetobacter calcoaceticus can directly decompose oil contaminants into a nutrient material for utilization and growth and reproduction, and has remarkable oil degradation effect. The invention also provides a high-density microbial inoculum prepared by mixing the Acinetobacter calcoaceticus and other components, the microbial inoculum can rapidly decompose the oil, and the other components in the microbial inoculum can improve the microecological environment of soil, and accelerate the soil mediation progress.

The invention discloses bacillus pumilus and application thereof, which belong to the technical field of microbes. The produced strain of the invention is named Bacillus pumilus Van35 according to classification, and the preservation number of the strain is CGMCC No.3411. When the strain preparation is spayed, the soluble total sugar content and the reducing sugar content of tobaccos can be increased, while protein, total nitrogen and nicotine content are reduced. Therefore, specific values of the ratio of dextrose to nitrogen, the ratio of total sugar to betaine and the ratio of total nitrogen to total alkaloid tend to rise, the quality of chemicals is high and the quality of the smoke panel test is improved.

The invention discloses a preparation method of microbial pesticide for controlling vegetable fungal diseases, belonging to the microbial technical field, which utilizes a streptomyces diastatochromogenes D isolated and screened from a soil sample to prepare various formulation pesticides with the steps of the activation cultivation of strain, fermentation cultivation, leavening extraction and distribution of pesticide auxiliary material to the purified active composite, wherein the soil sample is acquired from Lin' an Tianmu mountain in Hangzhou, Zhejiang. The preparation method of microbial pesticide for controlling vegetable fungal diseases is safe and can be used for controlling cucumber fusarium wilt, tomato gray mold, and other vegetable fungal diseases; and the controlling effect is a little better than normal chemical agents.

The invention discloses a process for treating chemical industrial tail water by an immobilized microbe-artificial wetland, that is, the tail water of the integral waste water which is from a chemical industrial park and is treated by an active sludge method enters the process device so as to reach the aim of further removing COD, N, P and various organochlorine pollutants in the tail water. Advantageous bacteria species which are fixed in a microcapsule by an immobilized microbe technology are used for degrading organochlorine pollutants in the chemical industrial tail water, thereby improving the treatment effects of the artificial wetland on the organochlorine pollutants, reinforcing the impact load capability of an artificial wetland system for resisting the organochlorine pollutants, and reducing the impact of the organochlorine pollutants on the artificial wetland micro-ecological environmental system. The process has the advantages that the process makes use of the combined actions of the immobilization and an artificial wetland technology, and strengthens the sewage treatment effects, and the treated waste water not only can discharge after reaching standards, but also can reduce the pollution of the organochlorine pollutants on the environment.

The invention belongs to the technical field of microorganism, in particular to a compound microbial preparation used for directly decomposing crop straws, namely an efficient straw-decomposing inoculant. The efficient straw-decomposing inoculant comprises the following raw materials by weight percentage: 20-30 percent of bacillus subtilis, 10-15 percent of white light gray streptomycete, 5-10 percent of endomycopsis fibuligera, 5-10 percent of candida utilis, 10-15 percent of aspergillus, 10-15 percent of paecilomyces lilacinus, 10-15 percent of trichoderma harzianum and 20-30 percent of thermophilus streptomycete. The efficient straw-decomposing inoculant is organically combined with soil no-tillage, shifting and fallow to achieve the comprehensive purposes of protecting the environment, improving soil, increasing the utilization rate of fertilizers, increasing the quantity and the quality of agricultural products and developing the ecological agriculture.

The invention relates to a spire morchella strain screening and bacteria species manufacturing method. It belongs to microorganism technique. The strain morchella conica M0503 is preserved at Chinese microscobial preservation management committee common mircens on August 11th, 2005 year. Its preservation number is CGMCC NO.1437. The formed liquid and solid bacteria species can be used to protect morchella wildlife resource, and increase its nature yield and quality. Thus it has great social, economic, and ecological benefits and broad application prospect.

This invention relates to new consolidation sand method with terraneous nitrogen fixation blue-green algae, which is completed by the following steps: blue-green algae foster, inoculation environment, inoculation time and inoculation quantity, and it uses the microorganism technology such as terraneous nitrogen fixation blue-green algae to achieve the fast consolidation of the flowing sand in the fixed and the half fixed desert area and achieve the ecology rehab in the engineering trouble area of the fixed and half fixed desert area. The applied nitrogen fixation blue-green algae can do photosynthesis by itself by the limited precipitation in desert, it grows up, and produces hull, which achieves the purpose of adding organic matter in soil and improving the structure of the soil and preventing and consolidating sands.

The invention relates to a trichoderma pseudokoningii (SMF2) strain and application thereof, which belong to the technical field of microbiology. The invention discloses the trichoderma pseudokoningii (SMF2) strain preserved in China Center for Type Culture Collection on February 24, 2009 with the preservation number being CCTCCNO: M209031, and the application of the strain to preparation of soil microbial agents of trichoderma preparations. The trichoderma pseudokoningii (SMF2) strain disclosed by the invention can be used for producing plant disease fungus cell wall digestive enzymes (CWDEs) and antibiotics of peptaibols at high yield, the CWDEs and the peptaibols cooperate in situ, and the inhibition effect of the trichoderma pseudokoningii strain to the plant disease fungus is further enhanced.

The invention discloses a bacillus amyloliquefaciens strain as well as a preparation method and application of a strain powder preparation of the bacillus amyloliquefaciens strain, and belongs to the technical field of microorganisms. The bacillus amyloliquefaciens JSSW-LA, which is obtained through screening, is preserved in China Center for Type Culture Collection with preservation number of CCTCC NO: M2015602. The strain powder preparation of the bacillus amyloliquefaciens, which is prepared through freeze-dried culture activation, fermentation culture and strain powder preparation, is applicable to an additive of fishery breeding feed or a water quality improver of aquaculture; the bacillus amyloliquefaciens JSSW-LA provided by the invention is capable of effectively inhibiting the growth of A.sobria, A.hydrophila, A.caviae, aeromonas veronii and edwardsiella tarda, and an inhibitory effect on the A.sobria, A.hydrophila and A.caviae is more excellent than that on other pathogenic bacteria. Therefore, the JSSW-LA strain powder, applied to aquaculture, is capable of effectively preventing and controlling diseases in aquaculture animals caused by pathogenic bacteria, in particular bacterial bleeding disease, so as to protect the healthy growth of aquatic animals.

The invention belongs to the technical field of microorganisms, and particularly relates to a biocontrol strain, namely trichoderma asperellum T-1, for highly producing composite cellulose degrading enzyme. The trichoderma asperellum T-1 is preserved at the China General Microbiological Culture Collection Center on September 24, 2014; and the preservation number is CGMCC 9722. The invention also provides a waste composting bactericide prepared from the trichoderma asperellum T-1 and an application of the waste composting bactericide in treatment of the organic waste. The trichoderma asperellum T-1 provided by the invention is rapid in growth, high in spore-producing ability, and high pH tolerance; a plurality of cellulose materials can be taken as substrates; and the trichoderma asperellum T-1 is a member of biocontrol bacteria, and can be applied to planting of crops to prevent diseases. By adding the trichoderma asperellum T-1 provided by the invention to the organic waste, the penetrating fluid generated by waste composting can be reduced; the degradation efficiency of the cellulose materials in the waste can be improved; and the quality of the waste composting product is improved.

The present invention discloses one kind of composite microbial fertilizer and its preparation process, and belongs to the field of applied microbial technology and fertilizer producing technology. The composite microbial fertilizer is prepared with microbial preparation, fermented chicken excrement, chemical fertilizer comprising urea, calcium biphospahate and potassium chloride in certain proportion, and bentonite, and through mixing and pelletizing. The microbial preparation consists of brown nitrogen fixing bacteria, cereour bacillus, giant bacillus and jelly-like bacillus as well as certain amount of dextrin. The present invention has the advantages of easy bacteria seed culture, simple preparation process, etc.

The invention belongs to the technical field of microorganisms, and discloses a microbial preparation for processing a coal chemical wastewater. The microbial preparation is prepared by the following methods: 1) preparing microbial nutriments; 2) preparing a microbial carrier; and 3) preparing the microbial preparation. The microbial preparation disclosed by the invention contains a plurality of microorganisms, so that the microbial preparation is reasonable in compatibility and low in investment cost, and can be put into large-scale promotion and application.

The invention discloses a Saccharomyces cerevisiae strain with high yield of beta-phenylethanol and application thereof, and belongs to the technical field of industrial microorganisms. The Saccharomyces cerevisiae strain with high yield of beta-phenylethanol is collected in China Center for Type Culture Collection on December 16, 2016, under CCTCC NO: M 2016785. The strain has capacity of high yield of beta-phenylethanol and is applied to yellow rice wine fermentation, cooking wine fermentation, vinegar brewing, soy sauce fermentation and baijiu fermentation, with beta-phenylethanol contents being 410mg / L, 450mg / L, 300mg / L, 200mg / L and 110mg / L respectively. Besides, flavor substances such as acetic acid-2-phenethyl ester are highly yielded. The Saccharomyces cerevisiae strain is good in fermentation performance, the content of beta-phenylethanol in fermented products is obviously increased, and fermented product quality is improved, so that the strain has a promising application prospect.

The invention provides bacillus velezensis as well as a separation screening method and application thereof, and belongs to the field of microbe technology and food processing. The invention providesthe bacillus velezensis with high fermentation capability; the bacillus velezensis is obtained through screening; during the fermentation, the capability of high yielding protease, amylase and fermented bean plasmin is realized; the bacillus velezensis can be applied to food fermentation in a large scale when being prepared into a food fermentation strain; fermentation food obtained through fermentation by using the bacillus velezensis, the contents of free amino acid and douchi fibrinolytic enzyme are high; the nutrition is rich; the characteristics of easy digestion, thrombus prevention andthe like are realized.

The invention discloses a method for preparing fermented virus-free cottonseed meal feed protein. The invention relates to a production process which uses cottonseed meal as raw material and produces the virus-free cottonseed meal feed protein by microbial technology in a transformation way, in particular to a microbial fermenting process of using multi-strains mixed solid to ferment and prepare virus-free cottonseed meal. Fermentation cultures of one strain Saccharomyces cerevisiae which is cultured in an amplification way, one strain Candida tropicalis, one strain Bacillus ubtilis and one strain Lactobacillus are evenly mixed in the proportion of 2 to 1 to 1 to 1 and are inoculated in cottonseed meal (through 40 mesh sieve) with 5 percent of the inoculation amount, wherein, the water content of the cottonseed meal is 50 percent; simultaneously, substrate culture media (5 percent of molasses, 5 percent of bran, 0.5 percent of ammonium sulfate, and the initial pH value is 6.0) are added, evenly mixed, fermented for 72hours at 30 DEG C in aerobic way, dried and sampled at 60 DEG C; finally, the nutrient analysis is carried out on the cottonseed meal before and after fermentation; when weighed and packaged, finished products of the fermented cottonseed meal can be stored for 6 months at a cool and dry place. The fermented cottonseed meal can be used as feeds for beef cattle, chicken, tilapia, etc.

The invention belongs to the technical field of microbes, and in particular relates to an antimicrobial compound and a fermenting preparation method and application thereof. The molecular weight of the antimicrobial compound of the invention is 445, the molecular formula of the antimicrobial compound is C16H23N5O10, the chemical name of the antimicrobial compound is 1-uracil-4-sarkosyl-serylamido-1,4-dideoxy-beta-D-glucopyranosyl acid, and the antimicrobial compound belongs to uracil hexose dipeptide and is named xin'aomycin. The preparation method adopts actinomycetes Streptomyces noursei and genetically improved strains thereof, and comprises the following steps: culturing in a primary liquid culture medium as a seed liquid; inoculating the cultured seed liquid to a secondary liquid culture medium B for culturing, and carrying out fed-batch fermentation culture of fed-batch liquid C; and after fermentation is finished, separating and purifying the structural compound from the fermentation culture liquid. The antimicrobial compound is used for manufacturing pesticide or medicine products. The invention has the characteristics of high yield, simple process and easy industrialization.

The invention discloses a breeding method for improving disease prevention ability of doves. A traditional dove breeding method does not pay attention to the improvement of the disease prevention ability of the doves, and therefore survival rates, breeding rates and growing speed of the doves are influenced in different degrees. According to the breeding method for improving the disease prevention ability of the doves, the doves are bred by a normal method, and meanwhile, a microbial technology can be used for carrying out environmental improvement and biological control. The microbial technology and Chinese medicine annexing agent can be used for improving internal conditions of the doves and the disease resistance capacity of the doves. The method can be used for breeding the doves, chemical drugs are basically not used in the whole breeding process by observing 30 thousands of pairs of the doves and the doves grow healthily. Compared with the normal breeding method, dove babies need 25-28 days to grow up to fly out of the bars and 22-25 days are just needed to breed the doves when the breeding method for improving the disease prevention ability of the doves is used; when the normal breeding method is used, female doves are mated with male doves to give birth to 1.5 dove babies every month, and when the breeding method for improving the disease prevention ability of the doves is used, the female doves are mated with the male doves to give birth to 1.7-1.8 dove babies every month; when the normal breeding method is used, the qualification rate of the eggs laid by the female doves is 98%, and when the breeding method of improving the disease prevention ability of the doves is used, the qualification rate of the eggs laid by the female doves is 99%. Therefore, the breeding method of improving the disease prevention ability of the doves is capable of improving the disease prevention ability of the doves.

The invention belongs to the technical field of the microorganism, and in particular to a treatment method of soil contaminated by radioactive nuclide uranium or cesium. The invention adopts a bar screen, a double-plate screen and a spiral screen to screen the soil contaminated by uranium or cesium. The soil particles with the particle size more than 0.25mm are clean soil particles, the soil particles with the particle size less than 0.25mm are soil contaminated by uranium or cesium; and the soil particles with the particle size less than 0.25mm are mixed with active acidithiobacillus ferrooxidans for treatment, and the settlement is clean soil after filtration, the filtrate is transferred and mixed with active sulfate reducing bacteria for treatment, then the uranium or cesium is precipitated and can be recovered through filtering separation, and the water can also be recovered. The invention has the advantages of simple operation and low cost, and realizes the rapid and efficient removal of the uranium or cesium in the slightly contaminated soil.