381 results about "Candida tropicalis" patented technology

The invention discloses a strain producing long chain dibasic acid, which is candida tropicalis CAT N145 with a collection number of CCTCC M 2011192. The invention also discloses an application of the strain and a method for producing dibasic acid by using the strain. The candida tropicalis CAT N145 provided by the invention has high conversion capacities upon n-alkanes, fatty acids, and fatty acid derivatives with different carbon chain lengths, and mixtures thereof. Miscellaneous acid content of the produced dibasic acid is low. The application range of the candida tropicalis CAT N145 is wide. With the strain and the application, the controlling over a fermentation process can be greatly simplified, and extraction and purification are easy. The strain has good market application prospect.

The invention relates to a decomposition maturing agent for degrading straw. The agent comprises active ingredients, such as candida tropicalis, aspergillus oryzae, trichoderma viride and bacillus subtilis. The decomposition maturing agent can rapidly decompose wheat straw, rice straw, grain straw, sweet potato stems, broad bean stems, rape stalks, weeds, leaves and household garbage of which the fibrous matter content is high so as to prepare an organic fertilizer. The decomposition maturing agent has the advantages of short degradation time and capability of effectively killing diseases and pests and providing nutrients for crops.

The invention relates to genetically engineered Candida tropicalis cells, use thereof and a method of production of ω-hydroxycarboxylic acids and ω-hydroxycarboxylic acid esters.

The invention relates to a bacterial preparation for degrading petroleum and restoring petroleum polluted soil ecology and a preparation method thereof. The bacterial preparation for degrading the petroleum and restoring the petroleum polluted soil ecology is a composite colony consisting of bacillus megaterium, pseudomonas fluorescens, streptococcus faecalis and candida tropicalis through optimized combination, wherein the matching ratio of the components is 1-2:1:1:0.5-1. The bacterial preparation has the advantage that the bacterial preparation can quickly form a micro-ecological advantage colony after the bacterial preparation is applied to the soil. If 2 to 5 percent of the bacterial preparation is added relative to the weight of surface soil (a cultivation layer of about 15 to 20 centimeters), the oil removal rate in three months is more than or equal to 75 percent (dichloromethane reflux extraction and gravimetric method), and the bacterial preparation has high effective colony number and strong adaptability and has remarkable effect of restoring the petroleum polluted soil ecological environment.

The invention relates to genetically engineered Candida tropicalis cells, use thereof and a method of production of ω-hydroxycarboxylic acids and ω-hydroxycarboxylic acid esters.

The invention relates to a method for producing a protein feed by using gingko leaf residue as a raw material for mixed strain solid-state fermentation. Activation and multiplication culture are respectively performed on candida tropicalis, aspergillus oryzae, bacillus subtilis and lactobacillus plantarum, so as to obtain four liquid strains; after the gingko leaf residue is dried and smashed, a certain amount of water and a nitrogen source are added, and after mixing and sterilizing, the gingko leaf residue is taken as a solid-state fermentation culture medium; the four liquid strains are inoculated to the solid-state fermentation culture medium according to certain proportions, after sufficient mixing, fermentation for 72 to 120 hours can be performed at a nature pH value under the temperature of 28 to 30 DEG C, and after drying, a gingko leaf residue protein feed can be obtained. In the protein feed, the coarse protein content of the protein feed is high and can reach 46 percent, the nutritional ingredients are complete, the palatability is excellent, the production performance and the feed conversion rate of livestock and poultry can be effectively improved, the preparation method is simple in process, the cost is low, and the mass production can be achieved. The invention further provides a way for using the gingko leaf residue which is a waste material in forestry processing.

The invention relates to a method for producing a microbial feed additive by using potato vinasse. The method comprises the following steps of: preparing a vinasse culture medium; mixing bacillus subtilis, trichoderma koningii and bacterial suspension liquid of trichoderma viride spores or mouldy bran, and adding into the vinasse culture medium for culturing and fermenting; and inoculating candida tropicalis or candida utilis seed liquid in the well-fermented culture medium for continuously culturing and fermenting; and finally drying to obtain the microbial feed additive. The invention has the advantages of low energy consumption, low cost, short production period, high production efficiency and recycled resources, reduces the emission of pollutants and is beneficial to building an environment-friendly and resource-saving society; and the produced additive has wide application range, can be added into animal feeds and is a high-quality and highly-efficient microbial fermentation product.

The invention provides a compound microorganism agent for treating a black and odorous water body. The compound microorganism agent comprises compound bacteria and a compound enzyme preparation, wherein the compound enzyme preparation comprises candida tropicalis, candida utilis, bacillus natto, bacillus subtilis, bacillus licheniformis, nitrobacter, achromobacter denitrificans, nitrosomonus, acinetobacter, lactobacillus, aspergillus niger, aspergillus oryzae and photosynthetic bacteria; the compound enzyme preparation comprises protease, amylase, lipase, sucrase, and cellulase.

The invention relates to compound microbial agents, bacterial manure, preparation methods thereof and application thereof to restoration of saline alkali soil .In particular, the compound microbial agents comprises the bacillus megaterium microbial agent, the pseudomonas stutzeri microbial agent, the rhizobium radiobacter microbial agent, the candida tropicalis microbial agent and the lactobacillus delbrueckii microbial agent .The invention further comprises the bacterial manure containing the microbial agents, the preparation methods thereof and application thereof to restoration of the saline alkali soil .By means of the compound microbial agents, the bacterial manure, the preparation methods thereof and application thereof to restoration of the saline alkali soil, raw materials which pollute the environment easily or need to be disposed such as straw, excrements of livestocks, household garbage and sludge can be utilized sufficiently, in addition, the compound microbial bacterial manure capable of restoring the saline alkali soil can be prepared, thus the immune function and stress resistance of plants can be improved, the land salinization degree can be improved, and the purpose of achieving second ploughing of salinized soil is achieved.

The invention provides a high-efficiency compound microbial inoculum for fermenting beds, particularly relates to a compound microbial inoculum for the fermenting beds, and a preparation method and application thereof. The compound microbial inoculum is composed of five beneficial bacterium strains (Bacillus subtilis CGMCC1.769 and CGMCC1.210, lactobacillus plantarum CICC6073, Candida tropicalis CGMCC2.587 and Trichoderma koningii CGMCC3.4290) and metabolites thereof (amylase, protease, lipase, cellulase, organic acid, bacteriophage and the like). The compound microbial inoculum is prepared by using a submerged liquid mixed fermentation technique. The method comprises the following steps: carrying out liquid submerged fermentation on the lactobacillus plantarum at 35 DEG C for 12 hours, respectively inoculating Candida tropicalis and Trichoderma koningii, carrying out mixed fermentation at 30 DEG C for 12 hours, inoculating Bacillus subtilis, carrying out liquid submerged mixed fermentation for 24 hours, respectively controlling the fermented viable count at 1-2*10<9> cfu / mL, 5-8*10<8> cfu / mL, 3-5*10<8> cfu / mL, 3-5*10<9> cfu / mL and 2-5*10<9> cfu / mL, finishing fermentation, packaging and sealing to obtain the high-efficiency compound microbial inoculum for the fermenting beds. The invention also provides application of the high-efficiency compound microbial inoculum-a preparation method of padding material. The compound microbial inoculum has the advantages of high viable count, high biological activity and low impurity bacterium pollution; the compound microbial inoculum can obviously reduce the odor of pigsties and reduce the living possibility of mosquitoes and flies, thereby achieving the ecological culture goals of no pollution and zero discharge; and the process for manufacturing the ferment bed is simple, thereby saving the padding material, human power and the like.

The invention discloses a method for producing long-chain alpha,omega-dicarboxylic acid by fermenting and converting an n-alkane with microbes, particularly a method for producing high-yield heptadecanedioic acid, which comprises the following steps: inoculating seed liquor, which is cultured from a Candida tropicalis mutant strain ly-7 which is obtained by mutagenesis by an N<+> injection technique and subjected to oxidase activity screening and culture, into mixed liquor of an n-alkane and a fermentation medium; fermenting and converting at 25-32 DEG C for 48-168 hours; and separating and purifying the generated long-chain alpha,omega-dicarboxylic acid. When n-heptadecane is fermented and converted to produce the heptadecanedioic acid in a 5-ton fermentation tank for 144 hours, the content of the heptadecanedioic acid can reach 163 g / l.

The invention discloses a method for biosynthesizing and producing long-chain Alpha, Omega-dicarboxylic acid, in particular relates to a high yielding method of a C11-C14 mixed long-chain dicarboxylic acid (DCm). A used microorganism is a mutant new strain ly-m of a Candida tropicalis. The method is characterized in that culture media using each single normal alkane of C11-C18 and light wax oil as substrates are respectively connected to strains of the microorganism at first; on the first stage, a system is controlled to mainly grow cells, and also produce a certain quantity of dicarboxylic acid; on the second stage, the fermentation system is controlled to mainly produce acid and also grow cells; and on the third stage, the system is controlled to only produce the acid without growing cells. When the method is used for fermenting 200# light wax oil (a normal alkane mixture of C11-C14) to produce the DC11-DC14 mixed long-chain dicarboxylic acid (DCm), the Candida tropicalis ly-m and the 200# light wax oil are fermented in a fermentation tank with a volume of 210m3 for 160 hours, and the content of DCm in a fermentation clear liquid can reach 175g / L.

The invention provides a heavy metal contaminated soil microbe repairing agent which comprises the following components: brevibacillus borstelensis, candida tropicalis, nutrients and a carrier. The heavy metal contaminated soil microbe repairing agent is used in the heavy metal contaminated soil repairing microbe, the microbe used in the heavy metal contaminated soil microbe repairing agent can rapidly colonize and grow in soil and form a strong colony, can directly adsorb heavy metal ions into the microbial cell walls or cells for curing and passivation, heavy metal mobility is decreased, and crop heavy metal ion absorption can be reduced. The technology is low in repair cost and convenient in construction, provides a new method for soil microbe repairing, and has good application value.

Methods and compositions useful in the detection and identification of species of Candida are disclosed. These species include Candida albicans, Candida glabrata, Candida parapsilosis, and Candida tropicalis, each of which is a causative agent for vaginal candidiasis. The compositions of the invention are combinations of oligonucleotides. These oligonucleotides include pairs of forward and reverse primers for polymerase chain reactions, wherein each primer pair is capable of priming the synthesis of an amplicon specific to one of Candida albicans, Candida glabrata, Candida parapsilosis, and Candida tropicalis, but preferably is not capable of priming the synthesis of an amplicon specific to any of the other three species. In preferred embodiments, the forward primers of the primer pairs have identical sequences, while each reverse primer of the primer pairs has a unique sequence relative to all of the other reverse primers; or the reverse primers of the primer pairs have identical sequences, while each forward primer of the primer pairs has a unique sequence relative to all of the other forward primers. These unique primer sequences account for the species specificity of the resultant amplicons. The oligonucleotides also include probes capable of detecting these amplicons, and sequencing primers for determining, in primer extension reactions, the nucleotide sequences contained within the amplicons. In preferred methods of the invention, a biological sample is tested for the presence of at least one isolate of Candida albicans, Candida glabrata, Candida parapsilosis, and Candida tropicalis by isolating nucleic acid from the sample, attempting a polymerase chain reaction in a mixture containing this nucleic acid and a plurality of these primer pairs, ascertaining whether any amplicon is produced in the mixture using an oligonucleotide probe, and determining the sequence of any resultant amplicon using the sequencing primers. The detection of an amplicon indicates that the sample contains at least one isolate of Candida albicans, Candida glabrata, Candida parapsilosis, or Candida tropicalis, and the nucleotide sequence data is used to determine which of these four Candida species is present.

The invention discloses a microorganism and porous material composite deodorant and the preparing method, wherein the microorganism is candida tropicalis CCW-Y3 bacterial strain and Saccharomyces cerevisiae with cellular material as the carrier, the mass ratio of microorganism and porous material carrier is 4-6:1000. The invention has the microorganism with high deodorant property on the porous material, which avoids the absorption saturation of the carrier by absorbing the bad smell molecule to the surface with the porous carrier with high deodorant property and disintegrating the bad smell molecule with the microorganism absorbed on the carrier, wherein the composite deodorant is arranged in the ventilating flat dish, and is hanged in the upper space of animal house, breeding house or faecal, the material of the dish is wood, iron or bamboo ware.

The composite microecological animal preparation consists of Bacillus cereus 10-15 wt%, Bacillus subtilis 10-15 wt%, Bacillus licheniformis 10-15 wt%, Saccharomyces cerevisiae 1-5 wt%, Candida Tropicalis 1-5 wt%, photosynthetic bacterium 1-5 wt%, plant lactobacillus 0.5-3 wt%, acidophilic lactobacillus 0.5-3 wt%, ephebic bifidobacterium 1-3 wt%, Aspergillus niger 0.5-3 wt%, Aspergillus oryzae 0.5-3 wt%, viridin 0.5-3 wt%, oligosaccharide 5-10 wt% and carrier 30-50 wt%. Its preparation process includes the following steps: 1) high temperature resisting domestication of the said strain, drug resisting domestication, preservation and strain passage; 2) activating the preserved strain, fermentation, coating with microcapsule and drying; and 3) crushing dried strain, and mixing with oligosaccharide and carrier. The present invention is one kind of high efficiency green feed additive.

The invention discloses a method for preparing fermented virus-free cottonseed meal feed protein. The invention relates to a production process which uses cottonseed meal as raw material and produces the virus-free cottonseed meal feed protein by microbial technology in a transformation way, in particular to a microbial fermenting process of using multi-strains mixed solid to ferment and prepare virus-free cottonseed meal. Fermentation cultures of one strain Saccharomyces cerevisiae which is cultured in an amplification way, one strain Candida tropicalis, one strain Bacillus ubtilis and one strain Lactobacillus are evenly mixed in the proportion of 2 to 1 to 1 to 1 and are inoculated in cottonseed meal (through 40 mesh sieve) with 5 percent of the inoculation amount, wherein, the water content of the cottonseed meal is 50 percent; simultaneously, substrate culture media (5 percent of molasses, 5 percent of bran, 0.5 percent of ammonium sulfate, and the initial pH value is 6.0) are added, evenly mixed, fermented for 72hours at 30 DEG C in aerobic way, dried and sampled at 60 DEG C; finally, the nutrient analysis is carried out on the cottonseed meal before and after fermentation; when weighed and packaged, finished products of the fermented cottonseed meal can be stored for 6 months at a cool and dry place. The fermented cottonseed meal can be used as feeds for beef cattle, chicken, tilapia, etc.

The invention discloses a fluorescence quantitative PCR primer, a probe and a kit for detecting ordinary pathogenic fungi. A specific primer and a TaqMan probe are independently designed, and a fluorescence PCR detection method which can be used for simultaneously detecting 15 clinically ordinary pathogenic fungi, including 8 candida mycoderma bacteria (candida albicans, candida glabrata, candida parapsilosis, candida kefyr, candida sake, candida kruse, candida guilliermind and candida tropicalis), four aspergilli (aspergillus niger, aspergillus flavus, aspergillus terreus and aspergillus fumigates), cryptococcus, rhizopus oryzae and mucor circinelloides, is established. The method is relatively high in sensitivity and specificity, and has significant meanings for early diagnosis and treatment on invasive infections with fungi.

The present invention relates to a method of producing xylitol and arabinose at same time from hemicellulose hydrolysates, material rich in xylan is hydrolyzed by acid or enzymes to obtain hydrolysate mostly containing xylose and arabinose, then inoculated in Issatchenkia orientalis S-7 and / or Issatchenkia occidentalis LJ-3 to eliminate the inhibitor of microbes, after that / or at the same, inoculated a yeast of Candida tropicalis which can consume the glucose, transform the xylose to xylitol and can not consume the arabinose, and fermented, then fermented liquor containing xylitol and arabinose is obtained. After separation, xylitol with purity more than 99% and arabinose with high purity are obtained. After concentration and crystallization respectively, crystalline xylitol and crystalline arabinose are obtained respectively.

The invention relates to a preparation method and application of a compound leavening agent. The preparation method comprises the steps of: separating lactobacillus plantarum, lactobacillus bulgaricus, streptococcus thermophilus, saccharomyces cerevisiae, candida rugosa, candida tropicalis and fermented pichia pastoris, as a leavening agent, from naturally fermented panada to produce a strain so as to prepare freeze-dried strain powder; weighing 1-2 parts of lactobacillus plantarum, 1-2 parts of lactobacillus bulgaricus, 1-2 parts of streptococcus thermophilus, 3-5 parts of saccharomyces cerevisiae, 3-5 parts of candida rugosa, 3-5 parts of candida tropicalis and 3-5 parts of fermented pichia pastoris based on parts by weight, and uniformly mixing to obtain the compound leavening agent. When the compound leavening agent is applied to the cooked wheaten food, bacterial contamination can be effectively prevented, the sanitation of the cooked wheaten food is guaranteed, the quality of the cooked wheaten food is improved, the production cycle is shortened, a production process is controllable, and the preparation method of the compound leavening agent lays a foundation for industrial production.

The invention discloses a method for synchronously fermenting n-undecane (nC11) to highly yield eleven-carbon dicarboxylic acid (DC11) by utilizing a microorganism. The microorganism is Candida tropicalis ly-1, of which the preservation number is CCTCC (China Center for Type Culture Collection) NO. M 209223. The method is characterized in that within 28 hours after strains of the microorganism are connected to a culture medium using normal alkane as the substrate, the pH (hydrogen ion concentration) is controlled below 6.8, cell growth is taken as the principal task, and a certain amount of dicarboxylic acid is also produced; within 28 hours to 60 hours, the pH is controlled below 7.3, acid production is taken as the principal task, and a certain number of cells are also increased; and after 60 hours, the pH is controlled below 8.0, and various dicarboxylic acids are produced quickly. When the method is used for fermenting nC11 to produce DC11, the stains of the microorganism and nC11are fermented for 150 hours in a fermentation tank with a volume of 210m3; and the output of DC11 can reach up to 156g / L, the conversion rate can reach 86.7 percent, and the purity of DC11 can reach 98.2 percent.

The invention provides a preparation method of a compound bacteria fermentation bed. Firstly, five production ferments of candida tropicalis, lactobacillus plantarum, streptococcus thermophilus, Bacillu subtilis AS 1.108 and Bacillu subtilis ACCC 11062 are prepared, the living bacterium of each production ferment is not less than 10<9 > / ml, afterward, the five production ferments of lactobacillus are freeze-dried. According to the weight proportion of the freeze-dried bacteria powder, 2-3 parts of candida tropicalis powder, 2-5 parts of lactobacillus plantarum powder, 2-4 parts of streptococcus thermophilus powder, 1-3 parts of Bacillu subtilis AS 1.108 powder and 3-5 parts of Bacillu subtilis ACCC 11062 powder are taken out, well mixed and vacuum-packaged thereafter, thereby obtaining the compound bacteria. After that, the culture medium of the fermentation bed is made from 1 percent glucose, 2 percent rice hull (uncrushed), 15 percent water and 82 percent kibbled straw. The compound bacteria is added into the culture medium with the weight proportion of adding 10 grams compound bacteria into every 100 kilograms culture medium after being dissolved by a little warm water to be well mixed, and then tiled and compacted with a thickness less than 15cm. The compound bacteria fermentation bed can be completely fermented in 5 days under natural conditions. The use of the production of the invention can reduce the smell of the pigsty and the proliferation possibility of flies and mosquitoes, thus ensuring that the pigs can grow healthily and providing people with safe pork while protecting the environment.

The invention relates to a composite biological antimicrobial for preventing wilt and greensickness of cotton. The active components of the composite biological antimicrobial comprise Candida tropicalis, streptomyces microflavus, Trichoderma viride and Bacillus subtilis. The composite biological antimicrobial for preventing wilt and greensickness of cotton can reduce the seedling death rate effectively, has obvious preventing and treating effect on wild and greensickness of cotton and provides the cotton output.

The invention relates to a compound microbial preparation for treating urban river water pollution and a preparation method of the compound microbial preparation. The compound microbial preparation comprises the following microbial agents in parts by volume: a Lactobacillus acidophilus agent, a Candida tropicalis agent and a bacillus subtilis agent. The preparation method comprises the following step: uniformly mixing the agents, thereby obtaining the compound microbial preparation. According to the compound microbial preparation for treating urban river water pollution prepared by the preparation method disclosed by the invention, the number of active viable organisms is more than or equal to 4000 million CFU per gram, so that the compound microbial preparation is suitable for repairing and treating pollutions such as standard exceeding of content of COD and ammonia nitrogen in urban river water, river water contamination and smelliness, can rapidly repair an ecological environment of a water body, prevent secondary pollution of the water body, rapidly restore a self-purification function of the water body and clear up river bottom sludge and is a biological pollution control product with the advantages of high efficiency, rapidness, low cost and sustainable development.

The invention relates to a microorganism and a microbial fermentation process thereof, in particular to a chromium-rich yeast culture and a fermentation process thereof. The process is characterized by selecting and screening Candida tropicalis and saccharomyces cerevisiae tolerant of high chromium and carrying out production through a liquid-solid two-phase fermentation process. The process flow comprises that: high-chromium-tolerant (trivalent) strains are screened; the strains are progressively expanded and cultured; liquid fermentation is performed to transform inorganic chromium, wherein the content of the inorganic chromium in a culture medium is 0.05 to 0.4 percent, preferably 0.1 to 0.2 percent, and the time of fermentation and transformation is 12 to 48 hours, preferably 18 to 26hours; after the liquid fermentation is over, culture solution and a solid culture medium are mixed according to the weight ratio of 1:3-1.5:1, preferably 1:1, and are subjected to solid aerobic fermentation for 16 to 36 hours, preferably 16 to 24 hours; after the solid aerobic fermentation is over, solid anaerobic fermentation is performed to break the wall of yeast at a temperature kept between40 and 60 DEG C for 6 to 24 hours, preferably at 50 DEG C for 6 to 10 hours; and a finished product after drying contains organic chromium accounting for 0.05 to 0.3 percent. The proportion of transforming the inorganic chromium into the organic chromium by utilizing a technique of the invention is more than 85 percent, and no hexavalent chromium is detected. The chromium-rich yeast culture produced in the invention has the characteristics of simple process, low production cost, good product quality and the like, has the double function of organic chromium and probiotic feed, and can be used for feed of livestock, poultry and aquatic products.

The invention provides a cadmium contaminated soil microbe repairing agent which comprises the following components: brevibacillus borstelensis, candida tropicalis, nutrients and a carrier. The microbe used in the cadmium contaminated soil microbe repairing agent can rapidly colonize and grow in soil and form a strong colony, can directly adsorb cadmium ions into the microbial cell walls or cells for curing and passivation, cadmium mobility is decreased, crop cadmium ion absorption can be reduced. The technology is low in repair cost and convenient in construction, provides a new method for soil microbe repairing, and has good application value.

The invention discloses a method for producing a protein feed through hypsizygus marmoreus fungus chaff solid state fermentation and the protein feed being produced via the method for producing the protein feed through hypsizygus marmoreus fungus chaff solid state fermentation. According to the method, hypsizygus marmoreus is selected to cultivate the produced waste material-pollution-free hypsizygus marmoreus fungus chaff, the ground hypsizygus marmoreus is combined with bran, urea and the like, candida utilis, candida tropicalis and geotrichum candidum are inoculated according to a certain rate so as to ferment under an appropriate condition, and the feed which is rich in protein is obtained. After assay determination, the protein feed contains 20%-40% of crude protein which is increased by 1.5-4 times compared with the crude protein of unfermented hypsizygus marmoreus fungus chaff; the crude fiber content is between 15% and 18% which is reduced by 2-3.5 times of the crude fiber content of the unfermented hypsizygus marmoreus fungus chaff, so that the protein feed is a hypsizygus marmoreus fungus chaff protein feed which has rich nutrition, high protein and low fiber and is beneficial to the utilization and absorption of livestocks. By the method, the feeding cost of farmers can be lowered, waste utilization is realized, and an environmental protection function is obtained.

The invention discloses a method of yeast fermentation coproduction ergot sterol and glutathion, utilizing conspecific yeast bacterial fermentation to produce simultaneous two kinds of metabolite-ergot sterol and glutathion, the utilization bacterial of fermentation is Saccharomyces cerevisiae, candida utilis, candida tropicalis or engineering bacterial reconstructed by mutagenesis and gene engineering, the utilization yeast culture medium contain the carbon source such as glucose, cereal mash, sucrose molasses and so on, the nitrogen source such as maize milk, yeast powder, protease leather, carbonyldiamide, ammonia and so on, inorganic salt and the metallic ion that the yeast need. The invention utilizes fermentation coproduction ergot sterol and glutathion, simultaneously, accomplish high-density culture of the yeast cell, and make the biomass of yeast expression coproduction come up to the standard of the high level in individual fermentation ergot sterol and glutathion, full utilization culture medium substrate, increasing availability ratio of raw material, decreasing the cost of manufacture.

The present invention provides fatty alcohol oxidase (FAO) proteins and nucleic acid molecules encoding the FAO proteins. Also provided are analogs, derivatives, and enzymatically active fragments of the FAO proteins. Vectors and host cells comprising the nucleic acid molecules encoding the FAO proteins, analogs, derivatives and enzymatically active fragments thereof are also provided. In addition, FAO signature peptides and isolated nucleic acid molecules encoding the signature peptides are provided by the present invention. Methods of producing or increasing production of a subject FAO protein, methods for increasing aldehyde production during the second step of the ω-oxidation pathway of fatty acids, methods for increasing production of a ketone from an alcohol during the second step of the ω-oxidation pathway of fatty acids, and methods for increasing production of a dicarboxylic acid are also provided.

The invention relates to a method for producing a bio-protein feedstuff through solid-state fermentation of a dreg which is a silybum marianum dreg. The invention belongs to the technical field of solid-state fermented bio-protein feedstuffs. According to the invention, a mixed microbial agent composed of microbial strains of aspergillus niger and candida tropicalis is adopted. Certain amounts of urea, glucose and potassium dihydrogen phosphate are added into silybum marianum dreg, bran, and corn flour with a certain ratio, and the materials are mixed, such that a solid-state fermentation culture medium is obtained; fermentation is carried out for 48-120h under a temperature of 28-32 DEG C; the material is dried, and a bio-protein feedstuff is obtained. Crude protein content in the protein feedstuff can reach 42%, such that the feedstuff can be applied as an animal culturing protein feedstuff, and can be used for replacing fish meal, meat-bone meal, soybean meal, and the like. The preparation method is simple, and the cost is low. With the method, large-scale productions can be realized. The invention also provides a novel approach for high-efficiency comprehensive utilizations of silybum marianum resources.