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Compound microbial preparation for treating urban river water pollution and preparation method of compound microbial preparation

A technology for complex microorganisms and pollution control, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of lack of efficient strains and mature technologies, etc., to quickly restore the ecological environment of water bodies and prevent secondary pollution. The effect of secondary pollution and low cost

Active Publication Date: 2014-12-03
BIOTECH RES CENT SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The proportion of microbial remediation in pollution control is relatively small, mainly due to the lack of efficient strains and mature technologies

Method used

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  • Compound microbial preparation for treating urban river water pollution and preparation method of compound microbial preparation
  • Compound microbial preparation for treating urban river water pollution and preparation method of compound microbial preparation
  • Compound microbial preparation for treating urban river water pollution and preparation method of compound microbial preparation

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Experimental program
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Effect test

Embodiment 1

[0064] Lactobacillus acidophilus (Lactobacillus acidophilus), strain number CGMCC1.1854, was purchased from the General Microbiology Center of China Committee for the Collection of Microbial Cultures.

[0065] Candida tropicalis (Candida tropicalis), strain number CGMCC 2.2735, was purchased from China General Microorganism Culture Collection and Management Center.

[0066] Bacillus subtilis (Bacillus subtilis) YYW-1 is derived from the General Microbiology Center of the China Committee for Culture Collection of Microorganisms, and the culture preservation number is CGMCC NO.2396.

[0067] The preparation method of the composite microbial preparation for urban river water pollution control, the steps are as follows:

[0068] (1) Get Lactobacillus acidophilus, inoculate it in solid medium, and through activation culture, make activated Lactobacillus acidophilus; Then inoculate the activated Lactobacillus acidophilus in liquid medium I and carry out expansion culture; Inoculate ...

Embodiment 2

[0103] The preparation method of the composite microbial preparation of urban river water pollution control as described in embodiment 1, the difference is that,

[0104] In described step (1), fermentation culture step is as follows:

[0105] The Lactobacillus acidophilus seed solution was inoculated in the fermentation medium I according to the inoculum amount of 10% by volume, at a temperature of 37° C., a tank pressure of 0.02 MPa, a stirring speed of 110 rpm, and cultivation for 48 hours to obtain a Lactobacillus acidophilus preparation;

[0106] Described fermentation medium I component is as follows, is weight part:

[0107] 5 parts of molasses, 6 parts of brown sugar, 2 parts of glucose, 1 part of peptone, 1 part of dipotassium hydrogen phosphate, 0.5 parts of sodium chloride, and 84.5 parts of water; the concentration of bacteria obtained is 8.1×10 9 CFU / mL Lactobacillus acidophilus preparation;

[0108] In described step (2), fermentation culture step is as follows...

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Abstract

The invention relates to a compound microbial preparation for treating urban river water pollution and a preparation method of the compound microbial preparation. The compound microbial preparation comprises the following microbial agents in parts by volume: a Lactobacillus acidophilus agent, a Candida tropicalis agent and a bacillus subtilis agent. The preparation method comprises the following step: uniformly mixing the agents, thereby obtaining the compound microbial preparation. According to the compound microbial preparation for treating urban river water pollution prepared by the preparation method disclosed by the invention, the number of active viable organisms is more than or equal to 4000 million CFU per gram, so that the compound microbial preparation is suitable for repairing and treating pollutions such as standard exceeding of content of COD and ammonia nitrogen in urban river water, river water contamination and smelliness, can rapidly repair an ecological environment of a water body, prevent secondary pollution of the water body, rapidly restore a self-purification function of the water body and clear up river bottom sludge and is a biological pollution control product with the advantages of high efficiency, rapidness, low cost and sustainable development.

Description

technical field [0001] The invention relates to a compound microbial preparation for urban river water pollution control and a preparation method thereof, belonging to the technical field of environmental restoration. Background technique [0002] As an important part of the urban ecosystem, urban rivers are of great significance to the ecological construction of the city. However, with the continuous acceleration of urbanization, the urban population gathers, the economic activities become more frequent, the discharge of industrial wastewater and domestic sewage increases, and the garbage is discarded randomly, which makes the urban environment polluted and the water quality deteriorates. The original clear river water has become black and dirty. The problem of environmental pollution is becoming more and more serious, seriously restricting the sustainable development of the city's society, economy and environment, and endangering people's health at the same time. [0003]...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N1/20C02F3/34C12R1/23C12R1/74C12R1/125
Inventor 岳寿松王翠萍邹晓凤游银伟王留名彭继敏郑建荣
Owner BIOTECH RES CENT SHANDONG ACADEMY OF AGRI SCI
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