64 results about "Candida rugosa" patented technology

The invention discloses a deodorant special for livestock and poultry farms and application thereof, wherein the deodorant is composed of the following strains: 106-108 lactobacillus acidophilus per milliliter, 107-109 lactobacillus reuteri per milliliter, 104-106 bacillus polymyxa per milliliter, 106-108 bacillus lentus per milliliter, 106-109 bacillus coagulans per milliliter, 104-106 enterococcus faecalis per milliliter, 107-109 enterococcus faecium per milliliter, 104-107 candida rugosa per milliliter, 104-107 candida valida per milliliter, 104-106 pseudomonas farinofermentans per milliliter, 106-108 rhodopseudomonas palustris per milliliter, 104-106 geotrichum suaveolens per milliliter, 106-108 aspergillus clavatus per milliliter, 106-108 rough spore aspergillus per milliliter and 106-109 streptomyces globisporus per milliliter. According to the invention, excrements in a poultry house and excrements for accumulative fermentation are treated through a spraying manner, so as to effectively reduce the release amount of malodorous gases, and provide a technical support for the effective treatment of malodor in the livestock and poultry farms and the environmental protection. Besides, the deodorant special for livestock and poultry farms can also be sprayed to the livestock and poultry body surfaces, to keep the livestock and poultry body surfaces clean and sanitary, and be conducive to the growth of livestock and poultry.

The invention relates to the technical field of a biological agent, and particularly relates to a cellulose degradation bacteria agent and a raw material strain, and preparation methods and applications of the cellulose degradation bacteria agent and raw material strain. The compound bacteria agent for degrading cellulose mainly comprises a paecilomyces varioti MM2 strain, an aspergillus fumigates MM6 strain, a candida rugosa MJ4 strain, and a bacillus licheniformis MX8 strain. The cellulose can be efficiently degraded and utilized by the compound bacteria agent disclosed by the invention, the compound bacteria agent can be used as a detonating agent and an enhancer of wine sediment compost, the compost process can be accelerated, the compost rotten degree is improved, an organic fertilizer is fabricated, and the cellulose degradation bacteria agent is especially applicable to resourceful treatment of lignocelluloses solid waste and production of the organic fertilizer by using the wine sediment.

The invention provides a heterotrophic nitrification-aerobic denitrification Candida rugosa strain and its application, belonging to the technical field of functional microorganisms. Heterotrophic nitrification and aerobic denitrification Candida rugosa (Diutina rugosa) strain DW‑1, the deposit number is CCTCC NO: M2019166. The invention also provides a microbial preparation for degrading ammonia nitrogen and nitrite nitrogen in freshwater aquaculture sewage comprising the bacterial strain DW‑1. DW-1 strain has the function of heterotrophic nitrification-aerobic denitrification, and can directly convert most of the ammonia nitrogen in nitrogen-containing sewage into gas products through heterotrophic nitrification-aerobic denitrification, and the accumulation of nitrite and nitrate nitrogen less; ammonia nitrogen or nitrite nitrogen can be used as the only nitrogen source for metabolism to achieve high-efficiency denitrification, so the application of the Candida rugosa strain DW-1 or the microbial preparation in the denitrification of freshwater aquaculture water.

The invention relates to a preparation method and application of a compound leavening agent. The preparation method comprises the steps of: separating lactobacillus plantarum, lactobacillus bulgaricus, streptococcus thermophilus, saccharomyces cerevisiae, candida rugosa, candida tropicalis and fermented pichia pastoris, as a leavening agent, from naturally fermented panada to produce a strain so as to prepare freeze-dried strain powder; weighing 1-2 parts of lactobacillus plantarum, 1-2 parts of lactobacillus bulgaricus, 1-2 parts of streptococcus thermophilus, 3-5 parts of saccharomyces cerevisiae, 3-5 parts of candida rugosa, 3-5 parts of candida tropicalis and 3-5 parts of fermented pichia pastoris based on parts by weight, and uniformly mixing to obtain the compound leavening agent. When the compound leavening agent is applied to the cooked wheaten food, bacterial contamination can be effectively prevented, the sanitation of the cooked wheaten food is guaranteed, the quality of the cooked wheaten food is improved, the production cycle is shortened, a production process is controllable, and the preparation method of the compound leavening agent lays a foundation for industrial production.

The patent discloses a method of applying immobilized lipase to catalyze ester hydrolysis for kinetic resolution of 2-(4-methylphenyl) propionic acid enantiomers. A bifunctional compound glutaraldehyde is used to react with a carrier UIO-66-NH2 and an amino group on candida rugosa lipase (Lipase AYS) at the same time to form a Schiff base to realize a covalent link of the enzyme and the carrier toobtain the immobilized enzyme which is used for the kinetic resolution of the 2-(4-methylphenyl) propionic acid enantiomers. Optically pure 2-(4-methylphenyl) propionic acid is an important pharmaceutical intermediate. The provided preparation method has obvious technical advantages. The covalently linked immobilization has stronger binding force, can reduce loss of the enzyme and maintains activity and selectivity of the enzyme. Compared with free enzymes, the immobilized enzyme is high in thermal stability, high in pH tolerance, easy for recycling for use and easy for separation from the product, significantly reduces technical cost, and is also simple in operation, green and environmentally protective.

The invention relates to the technical field of a biological agent, and particularly relates to a cellulose degradation bacteria agent and a raw material strain, and preparation methods and applications of the cellulose degradation bacteria agent and raw material strain. The compound bacteria agent for degrading cellulose mainly comprises a paecilomyces varioti MM2 strain, an aspergillus fumigates MM6 strain, a candida rugosa MJ4 strain, and a bacillus licheniformis MX8 strain. The cellulose can be efficiently degraded and utilized by the compound bacteria agent disclosed by the invention, the compound bacteria agent can be used as a detonating agent and an enhancer of wine sediment compost, the compost process can be accelerated, the compost rotten degree is improved, an organic fertilizer is fabricated, and the cellulose degradation bacteria agent is especially applicable to resourceful treatment of lignocelluloses solid waste and production of the organic fertilizer by using the wine sediment.

The invention discloses a novel chiral resolution method for obtaining a single enantiomer of 2-(4-methylphenyl)propionic acid, namely a method for synthesizing a single enantiomer of 2-(4-methylphenyl)propionic acid by enzymatically catalyzed stereoselective hydrolysis of 2-(4-methylphenyl)propionate enantiomers. Based on high selectivity and high catalytic efficiency of candida rugosa lipase, 2-(4-methylphenyl)propionate enantiomers are hydrolyzed to obtain (+)-2-(4- methylphenyl)propionic acid with high optical purity; based on the interaction of cyclodextrin to 2-(4-methylphenyl)propionateenantiomers, the conversion rate of 2-(4-methylphenyl)propionate is improved; the conversion rate of a substrate and the optical purity of the product can be as high as 40.76% and 96.24%, respectively, and the stereoselectivity E is as high as 145; the method overcomes the problems of low optical purity, low yield, environmental pollution and the like of general resolution technology, and can achieve high conversion rate and high selectivity of hydrolysis of 2-(4-methylphenyl)propionate enantiomers, so as to realize non-toxic, harmless and environmental-friendly resolution of chiral compoundsand mild reaction conditions; and the aims of simple equipment, easy operation, low cost and the like are achieved.

The invention relates to a method for preparing R-6-hydroxy-1-aminoindane through dynamic kinetic resolution. The method is characterized by using candida rugosa lipase as a biological resolution catalyst, D-(-)-O-acetyl mandelic acid as an acyl donor and a non-crystal nickel catalyst KT-02 as a racemization catalyst to carry out one-pot dynamic kinetic resolution reaction in an autoclave in the presence of hydrogen to convert 6-hydroxy-1-aminoindane to an R-6-hydroxy-1-aminoindane acyl compound and carrying out acid hydrolysis, alkali freeing, and the like on the acyl compound to obtain R-6-hydroxy-1-aminoindane with ee (enantiomeric excess) value more than 99%. The method has the characteristics of simplicity in operation, cheap and accessible racemization catalyst, complete utilization of the raw materials, high optical purity of the product, and the like, and has great guidance and application values in production and preparation of R-6-hydroxy-1-aminoindane.