123 results about "Rhodotorula species" patented technology

The present invention provides several methods for biological production of para-hydroxycinnamic acid (PHCA). The invention is also directed to the discovery of new fungi and bacteria that possess the ability to convert cinnamate to PHCA. The invention relates to developing of a new biocatalyst for conversion of glucose to PHCA by incorporation of the wild type PAL from the yeast Rhodotorula glutinis into E. coli underlining the ability of the wildtype PAL to convert tyrosine to PHCA. The invention is also directed to developing a new biocatalyst for conversion of glucose to PHCA by incorporation of the wildtype PAL from the yeast Rhodotorula glutinis plus the plant cytochrome P-450 and the cytochrome P-450 reductase into E. coli. In yet another embodiment, the present invention provides for the developing of a new biocatalyst through mutagenesis of the wild type yeast PAL which possesses enhanced tyrosine ammonia-lyase (TAL) activity.

The present invention provides several methods for biological production of para-hydroxycinnamic acid (PHCA). The invention is also directed to the discovery of new fungi and bacteria that possess the ability to convert cinnamate to PHCA. The invention relates to developing of a new biocatalyst for conversion of glucose to PHCA by incorporation of the wild type PAL from the yeast Rhodotorula glutinis into E. coli underlining the ability of the wildtype PAL to convert tyrosine to PHCA. The invention is also directed to developing a new biocatalyst for conversion of glucose to PHCA by incorporation of the wildtype PAL from the yeast Rhodotorula glutinis plus the plant cytochrome P-450 and the cytochrome P-450 reductase into E. coli. In yet another embodiment, the present invention provides for the developing of a new biocatalyst through mutagenesis of the wild type yeast PAL which possesses enhanced tyrosine ammonia-lyase (TAL) activity.

The invention relates to a method for preparing ocean rhodotorula fermentation feed. The method comprises the preparation of solid fermentation base stock of soybean meal, the mixing of a nutrition replenishing agent and the preparation of a solid fermentation medium. The feed is characterized in that ocean rhodotorula and a lactobacillus strain are inoculated in the solid fermentation medium and are subjected to solid fermentation to obtain the ocean rhodotorula fermentation feed. The method has the advantages: 1. macromolecular protein in the soybean meal is decomposed into small molecular peptide which improves the digestive absorption rate of the protein and further balances the composition of amino acid through microbial transformation; 2. the fermentation feed is replenished with astaxanthin and other biological active substances introduced by the metabolism of rhodotorula glutinis, thereby improving the nutritive value of the feed; 3. lactobacillus in the fermentation feed has positive function on improving a microecological flora of intestinal tracts of bred animal; and 4. the method has low fermentation cost, low fermentation condition and equipment requirement, simple operation of process flow and small investment and is suitable for small-scale production of breeding households and feed processing factories.

The invention discloses a method for improving the biomass and oil fat yield of microorganisms. The method comprises the following steps: (1) carrying out activated culture: respectively inoculating chlorella pyrenoidosa and rhodotorula glutinis cells into a culture medium, carrying out activated culture to a logarithmic phase, and taking a product as a seed solution; (2) respectively inoculating chlorella pyrenoidosa and rhodotorula glutinis into a shake flask according to different proportions, wherein each shake flask is internally filled with a culture medium, putting the shake flasks into a lighting shaker, and culturing; (3) respectively inoculating chlorella pyrenoidosa and rhodotorula glutinis cells into a BG11 culture medium, wherein the ratio of chlorella pyrenoidosa cell number to the rhodotorula glutinis cell number is equal to 3: 1, and the BG11 culture mediums contain 1.6g / L of yeast extract and have different glucose concentrations; putting the inoculated shake flasks into the lighting shaker, and culturing for 12 days; (4) after the culture is finished, centrifuging microbial cells, washing and carrying out freeze drying to obtain powder; the powder can be used for measuring and evaluating the dry weight concentration, total lipid content, fatty acid content and yield of the biomass. The method has the advantages that the biomass and oil fat yield of the oil-containing microorganisms are remarkably improved by utilizing a mixed culturing mode, and are 3-4 times or more than those of microorganisms cultured under a single culture condition; the method is relatively simple and convenient, and the energy consumption and the production cost are effectively reduced.

The invention discloses a water quality improver and a preparation method thereof. The water quality improver is prepared from the following raw materials in parts by weight: 30 to 40 parts of soybean, 40 to 50 parts of corn, 20 to 30 parts of composite biological enzyme, 15 to 25 parts of bacillus subtilis, 10 to 20 parts of enterococcus faecalis, 18 to 25 parts of photosynthetic bacteria, 10 to 18 parts of bacterium nitrobacter, 15 to 23 parts of oxygen producer, 17 to 23 parts of ion exchanger, 1 to 1.5 parts of wall-broken yeast powder, 22 to 30 parts of nano-silver, 11 to 15 parts of HA salt, 5 to 8 parts of microelement, 2 to 4 parts of vitamin, 5 to 10 parts of rhodotorula glutinis, and 33 to 45 parts of medical stone powder. The water quality improver provided by the invention is simple in preparation method, low in cost, and capable of reducing bottom mud organic matters of aquaculture water, thickening an aerobic layer, accelerating growth and reproduction of aerobes and decomposition of organic pollutants, and can provide micronutrient required for normal growth of aquatic products so as to improve the quality of the aquatic products.

The invention relates to a plant growth regulator and a method for producing the same. The plant growth regulator is characterized by comprising raw materials according to the following weight ratio: 1 to 20 weight portions of copper sulfate, 1 to 50 weight portions of zinc sulfate, 1 to 30 weight portions of manganese sulfate, 1 to 40 weight portions of ferric sulfate, 1 to 25 weight portions of sodium molybdate, 0.1 to 1 weight portion of amino acid, 1 to 20 weight portions of nucleic acid protein, 0.001 to 0.002 weight portion of vitamin E, 2 to 6 weight portions of agricultural rare earth, 1 to 30 weight portions of silicic acid, 5 to 20 weight portions of sugar, and 1,000 to 2,000 weight portions of water, wherein each one hundred weight portions of the regulator contains 0.5 weight portion of potato polyphenol oxidase complex, 0.5 weight portion of rhodotorula glutinis L-phenylalanine ammonia-lyase complex, 0.5 weight portion of earthworm superoxide dismutase, 0.5 weight portion of seaweed bioactive substances, and 0.5 weight portion of anti-pest composite.

The invention discloses a rhodotorula glutinis phenylalanine deaminase gene and an application thereof, and belongs to the field of biotechnology. Cloning of the phenylalanine deaminase gene plays an important role in biosynthesis of L-phenylalanine by an enzyme method. The phenylalanine deaminase gene is obtained by extracting rhodotorula glutinis RNA and carrying out reverse transcription to obtain the full-length phenylalanine deaminase gene, wherein the full length of the gene is 2121 bp, and 705 amino acids are encoded. Phenylalanine deaminase gene resources are further expanded, and a scientific basis is provided for molecular modification of phenylalanine deaminase and improvement of the stability and catalytic activity of enzymes.