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Method for treating industrial waste and fermentation production of microbial oil by microorganism as well as special strain thereof

A technology for microbial oil and industrial waste, applied in biological water/sewage treatment, biochemical equipment and methods, microorganisms, etc., can solve the problem of cost, which cannot be effectively solved, and the high cost of biodiesel

Inactive Publication Date: 2008-06-25
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the cost of producing biodiesel from animal and vegetable oils is too high, accounting for more than 75% of the total production cost, and there is also the problem of competing for land with agriculture in a large number of oily plants
Although microbial oil has solved the problem of competing for land with agriculture, if high-concentration glucose is still used as fermentation raw material, the cost problem cannot be effectively solved

Method used

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  • Method for treating industrial waste and fermentation production of microbial oil by microorganism as well as special strain thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Embodiment 1, the acquisition of Rhodotorula viscosus Rh.g CGMCC No.2258 bacterial strain

[0036] A series of mutagenized strains of Rhodotorula glutinis were obtained through multiple mutagenesis methods such as UV, EMS, LiCl and microwave, and various mutagenesis techniques, among which the optimal strain was selected and named as Rhodotorula glutinis. g, the specific mutagenesis process includes the following methods and steps:

[0037] 1) UV mutagenesis: the seed liquid of Rhodotorula glutinis (Rhodotorula glutinis) CICC No.1725 (purchased from China Institute of Food and Fermentation Industry) was cultured for 24-30h and then diluted 10 5 After doubling, spread on the yeast solid plate medium (glucose: 20%, yeast extract: 0.4%, urea: 0.2%, agar: 2%, pH4.5), respectively in the power of 15W, the irradiation distance is 30cm After irradiating for 10-600s under the ultraviolet lamp, incubate in the dark at 28-30°C for 3-5 days, check the growth of the colonies, and ...

Embodiment 2

[0044] Embodiment 2, produce microbial oil under the condition of supplementing glucose mother liquor with the mixed solution of sauce residue and papermaking wastewater

[0045] Seed liquid medium formula: glucose 4g, (NH 4 ) 2 SO 4 0.2g, KH 2 PO 4 0.7g, Na 2 SO 4 0.2g, MgSO 4 ·7H 2 O 0.15g, yeast powder 0.15g, dilute to 100mL with water, pH 5.5.

[0046] Preparation of fermentation medium: papermaking wastewater (papermaking wastewater contains a large amount of degradation products such as lignin and hemicellulose, pigments, pentoses, residual alkali and other leached substances, the quality of wastewater is: COD=106000~157000mg / L, BOD =34500~42500mg / L, SS=23500~27800mg / L, pH=11~13.) After centrifugal sedimentation to remove insoluble particles, add glucose mother liquor (glucose mother liquor is the waste liquid of glucose produced by starch factory, the main component is glucose, concentration 400~500g / l) to 2% (g / dL) of glucose content, and then added to 2% (...

Embodiment 3

[0053] Embodiment 3, use monosodium glutamate waste water to produce microbial oil under the condition of adding glucose mother liquor

[0054] Seed solution medium formula: glucose 3g, (NH 4 ) 2 SO 4 0.1g, KH 2 PO 4 0.5g, Na 2 SO 4 0.1g, MgSO 4 ·7H 2 O 0.1g, yeast powder 0.1g, dilute to 100mL with water, pH 6.

[0055] Preparation of fermentation medium: monosodium glutamate waste water (monosodium glutamate waste water mother liquor is a kind of low-acid high-concentration organic waste water, waste water water quality is: COD=50000~70000mg / L, BOD5=25000~35000mg / L, N-NH 3 =8000~10000mg / L, containing about 1.5% glutamic acid, pH=2~3. ) after centrifugal sedimentation to remove insoluble particles, add glucose mother liquor to a glucose concentration of 4%, adjust the pH value to 5-6 with KOH, and sterilize for use.

[0056] Fermentative production microbial oil with the method of the present invention, concrete method comprises the following steps:

[0057] 1) S...

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Abstract

The invention discloses a method for utilizing industrial wastes to produce microbial grease by means of fermentation and a special strain Rhodotorula glutinis Rh.g CGMCC No.2258. The method of the invention is a method for utilizing the industrial wastes (such as waste water of industrial production like starches, monosodium glutamate, paper making and so on, and / or fermentation industrial residues like soya residues and so on) as culture media to produce the microbial grease through fermentation of the Rhodotorula glutinis; the microbial grease obtained by the method also can be taken as material lipid to prepare biodiesel, and conversion rate can reach 80 to 99 percent. The production method of the microbial grease has the advantages of simple operation, low cost and high use value, and not only pollution of high concentration wastes which are discharged by fermentation industries like starches, monosodium glutamate, paper making, soya residues, etc. on the environment can be reduced but also the wastes can be converted into treasures by using the wastes to produce the grease which is a product in short supply in the current market, thereby the aim of resource utilization of the wastes is reached.

Description

technical field [0001] The present invention relates to the application field of microorganisms, in particular to a method for treating industrial wastes by microorganisms, and using industrial wastes (such as starch, monosodium glutamate, papermaking and other industrial production waste water and / or fermented industrial waste residues such as sauce residues) to ferment and produce microbial oils method and its special strains. Background technique [0002] With the accelerated development of industrial modernization, the discharge of industrial wastewater is also increasing and causing serious environmental pollution. At present, the annual output of starch wastewater in my country is about 24 million tons, and the output of high-concentration monosodium glutamate wastewater is more than 25 million tons per year. Among them, the discharge of paper industry wastewater in 2005 reached 3.67 billion tons. The common feature of these industrial wastewater is that it contains a...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N13/00C12N15/01C12P7/64C02F3/34C10G3/00
CPCY02E50/13Y02E50/10Y02P30/20
Inventor 谭天伟薛飞燕莫夏迪张栩
Owner BEIJING UNIV OF CHEM TECH
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