207 results about "Rhodotorula" patented technology

The invention discloses a Rhodotorula mucilaginosa strain and application in fermentation production of carotenoid and oils. The main point of the technical scheme involved in the invention lies in that: the Rhodotorula mucilaginosa strain is preserved in China General Microbiological Culture Collection Center with a preservation number of CGMCC No.8926. The Rhodotorula mucilaginosa provided by the invention can be used for fermentation production of carotenoid and oils. According to the invention, Rhodotorula mucilaginosa is utilized to produce carotenoid and oils at the same time, multi-round ARTP (atmospheric and room temperature plasma) mutagenesis is carried out on the production strain so as to obtain a high yield mutant strain, thus providing good application prospects and economic benefits for industrial production of carotenoid and oils.

An acid-resistant rhodotorula R30 (CGMCC No.1147) for removing heavy metals from sludge is disclosed. The process for removing heavy metals from sludge includes loading the sludge in bioreactor, inoculating said rhodotorula R30 and thiobacillus TS6, aerating at 28 deg.C for 2-6 days, dewatering of sludge, regulating pH of liquid phase for depositing heavy metals, and neutralizing solid phase for agricultural purpose.

The invention relates to ocean rhodotorula YS-185 bred with high yield of astaxanthin, which is obtained from ocean, wherein the rhodotorula is preserved in CCTCC of Wuhan, China with the preservation number of CCTCC NO: M207077; the invention also relates to a method for producing astaxanthin by fermenting and cultivating the ocean rhodotorula YS-185. The astaxanthin produced by the ocean rhodotorula YS-185 is natural astaxanthin and can be widely applied to high-quality cosmetics, food and feed additives.

Process for producing the enzyme D-amino acid oxydase of Rhodotorula gracilis in host cells. The process for the expression of the enzyme comprises isolating the complementary DNA corresponding to the messager RNA of the gene which codes for the D-amino acid oxydase of any strain of Rhodotorula gracillis producing said enzyme; fusing the fragment of DNA which codes for D-amino acid oxydase of Rhodotorula gracillis with a DNA sequence which contains a site of union to the ribosome and a high efficiency promoter sequence for the express of genes in host cells; inserting the DNA fragment into a plasmid appropriate for the host cell; cultivating the host cells transformed with said plasmid; and collecting the enzyme.

The invention provides a high-yield carotinoid and copper fermentation culture method by using Rhodotorula benthica, which is implemented by carrying out fermentation culture on the Rhodotorula benthica WYN1 in a copper-containing culture medium to efficiently produce carotinoid, wherein the fermentation culture medium is composed of 1% of glucose, 1-3% of enzymolysis corn flour, 2.5% of peptone, 0.02% of magnesium sulfate, 0.15% of potassium dihydrogen phosphate, 150-200 mg / L copper, 20% of sodium chloride and the balance of water. The method comprises the following steps: culturing at 24-28 DEG C for 10-15 hours, wherein the initial pH value is 8, the inoculum size is 3-8%, and the rotation speed of the shaking table is 50-80 r / min; regulating the temperature to 16-20 DEG C, carrying out low-temperature stationary culture, stopping stirring, regulating the pH value to 3-5, and keeping for 2-4 hours; carrying out gradient heating to 24-30 DEG C, regulating the pH value to 4.6-5.5, adding 2.5% of the peptone, and adding 2% of the yeast powder; and regulating the rotation speed of the shaking table to 50-100 r / min, and continuing fermentation for 10-15 hours.

The invention provides a stress-resistant feed for aquatic products, and a feed additive. The stress-resistant feed additive for aquatic products is mainly prepared from the following components in percentage by weight: 3-20% of a butyric acid derivative, 1-10% of marine rhodotorula, 0.2-5% of taurine, 5-20cfu / g of probiotics, 1-20% of a traditional Chinese medicine extract, 1-10% of complex vitamins and the balance of other auxiliary materials. The additive disclosed by the invention can notably improve the stress response resistance of aquatic animals, besides, the functions of the additive are diversified, the tolerance of the aquatic animals to various adverse environments of hypoxia, transportation and hyposaline, the adverse environment of being away from water and the like can be enhanced, and the growth of the aquatic animals can also be promoted.

The invention discloses rhodotorula mucilaginosa and a fermentation culture substance and application thereof. The rhodotorula mucilaginosa is rhodotorula mucilaginosa TZR<2014>, and the preservation number is CCTCC NO:M2015574. Liquid state fermentation is conducted on the rhodotorula mucilaginosa by means of the fermentation culture medium, the biomass of fermentation liquor is 18 g / L, and the yield of carotenoid is 36 mg / L. The rhodotorula mucilaginosa has the effects of resisting staphylococcus aureus, gastric acid and cholate, and a solid fermentation substance of the rhodotorula mucilaginosa further contains pyruvic acid which has the functions of accelerating fat consumption, increasing the muscle yield, phagocytosing free radicals in bodies and inhibiting generation of the free radicals. Therefore, the rhodotorula mucilaginosa can be used for feed additives of livestock, poultry and aquatic animals and has a wide application prospect.

The invention provides a novel suckling pig feed with traditional Chinese medicine preparation. The novel suckling pig feed is prepared from main materials and the traditional Chinese medicine preparation, wherein the main materials comprise corn, broken rice, extruded corn, extruded soybeans, extruded rice bran, soybean meal, fish meal, soybean oil, fermented soybean meal, organic calcium, monocalcium phosphate, sodium chloride, milk powder, piglet multi-vitamin bags, rhodotorula benthica, antibacterial peptides, bacillus natto, bacillus subtilis, bacillus licheniformis, clostridium butyricum, piglet special enzyme, phytasae, vitamin C, amino acid bags, an emulsifying agent, a mildew preventive, an antioxidant, a flavoring agent, organic multi-mineral, nanometer montmorillonite and choline chloride; and the traditional Chinese medicine preparation comprises dried orange peel, dandelion, radix scutellariae, liquorice, honeysuckle and golden cypress. The novel suckling pig feed with the traditional Chinese medicine preparation, provided by the invention, is suitable for feeding piglets in the earlier stage, is reasonable in nutrition combination and capable of promoting the growth speed of the piglets, shortening the growth cycle, reducing the morbidity and enhancing the immunity and is beneficial to the digestive absorption of the piglets.

The invention discloses a solid ocean red yeast preparation which is mainly prepared from a composite protective agent and ocean red yeast bacterial sludge according to a mass ratio of (1.5-2.2):1, wherein the cell density of ocean red yeast in the solid ocean red yeast preparation is 200-400 billion / gram, the cell activity is 70-80%, and the protective agent comprises skim milk powder and cane sugar. The invention further discloses a preparation method of the solid ocean red yeast preparation, The preparation method comprises the following steps: firstly, performing high-density cultivation on the ocean red yeast in a cultivation mode of continuous liquid flow layer ventilation, and further drying by using a vacuum freezing drying method. According to the solid ocean red yeast preparation, the survival rate of the ocean red yeast can be increased to be 70-80%, the cell density in the preparation can be 200-400 billion / gram, and the preparation can be also used in aquatic product feeds as an additive as a product of the preparation is in a solid manner, long in expiration date and convenient to transport.

The invention relates to a household sludge reduction compound microbial preparation as well as preparation and application methods thereof, belonging to the field of microbial application. Based on the total bacteria count, the household sludge reduction compound microbial preparation comprises 10-20% of Celloulonmonassp., 10-20% of Serratiasp., 30-60% of Bacilluspumilus and 20-30% of Rhodotorulamucilaginosa, wherein the Celloulonmonassp. is selected from at least one of CCTCCAB209393 and YMCCKMS3990, the Serratiasp. is selected from at least one of ACCC02490 and CICC20143, the Bacilluspumilus is ACCC10113 and the Rhodotorulamucilaginosa is CICC31192. The microbial preparation and the preparation method provided by the invention are simple and feasible, economic and reasonable and free from secondary pollution to the environment.

Disclosed are a cosmetic composition for improving anti-oxidation, anti-inflammation and atopic skin by using fermented liquid of black tea as an effective ingredient and a method of preparing the same. The cosmetic composition includes the fermented black tea liquid obtained by primarily fermenting black tea with saccharomyces, secondarily fermenting the black tea with gluconacetobacter and tertiarily fermenting the black tea with bacillus.

A starch-adsorption fluidized-bed process for drying the solid marine rhodotorula features use of continuous stream plus deep aeration culture and starch-adsorption fluidized-bed. The resultant has high concentration of rhodotorula cells (5-10 billions / g), high output and high living cell percentage (30%). It can be used to raise prawn and marine crab with high survial rate, health level and raising efficiency.

The invention discloses a stichopus japonicus seedling large-scale ecological culture method utilizing outdoor net cages. The method comprises the following steps that 1, the parent cucumber egg taking is adopted for incubation in a seedling raising workshop, and the breeding collection is carried out when seedlings grow to the early ear larva stage; 2, net cages are arranged in an offshore haven bay or a sea cucumber pond, and 200-to-300-mesh bolting-silk nettings are selected in the net cages; 3, collected early ear larvae are uniformly fed into the net cages; 4, the manual supplementary feeding of rhodotorula benthica is adopted; 5, when the early ear larvae grow to the big ear larva stage, substrata are hung in the net cages, and in addition, the manual supplementary feeding of rhodotorula benthica stops; and 6, the cucumber is raised to the young cucumber stage, the bolting-silk nettings are replaced into 60-80-mesh polyethylene nettings, and the cucumber is continuously raised. The method has the advantages that the offshore or pond net cage culture is carried out from the stichopus japonicus larva culture, so the goals of reducing the cost, improving the survival rate, improving the economic benefit and the like are reached.

The invention discloses a preparation method of an olive sauce rich in carotenoids. The method comprises the following steps: adding a proper amount of drinking water into olive fruit residue for moistening after squeezing oil, introducing steam for steaming, inoculating aspergillus niger bran koji, carrying out heat-preservation fermentation at the temperature of 30-33 DEG C, adjusting water content, carrying out heat-preservation fermentation at the temperature of 45-55 DEG C, then inoculating a rhodotorula bacteria seed solution, carrying out heat-preservation fermentation at the temperature of 30-33 DEG C, then carrying out heat-preservation fermentation for 48-60 hours at the temperature of 26-29 DEG C, then ending fermentation, mixing thick broad-bean sauce, olive oil, table salt, white granulated sugar and red square fermented bean curd juice, and decocting to prepare the olive sauce. The olive fruit residue after squeezing oil is subjected to enzymolysis by aspergillus niger, so that the content of tannin is reduced; starch and protein are degraded into micromolecule materials; the fragrance of the olive sauce is improved; the digestion and absorption rate of nutrition components is increased; through post fermentation of red yeast after pre-fermentation, the carotenoids are generated; the nutrition materials are increased; the thick broad-bean sauce, the olive oil and the red square fermented bean curd juice are used for decocting, so that the olive sauce is strong in sauce flavor and rich in taste.

The invention discloses a Rhodotorula mucilaginosa and the application thereof in the degradation and decoloring of dyes and the production of carotenoid. The Preservation No. of the Rhodotorula mucilaginosa JM401 is CCTCC NO: M 2013088; the saccharomycete can be used for carrying out degradation and decoloring on a triphenlmethane dye: and centrifugally collecting activated seed solution bacteria and vaccinating the bacteria to a decoloring medium containing the dye, and carrying out shake cultivation on the obtained product for 2-12 h at a temperature of 25-37 DEG C and a pH value of 4.0-7.5 so as to obtain a decoloring culture solution; and the saccharomycete can be used for producing carotenoids by fermentation: taking an activated seed solution and vaccinating the activated seed solution to a fermentation medium, and carrying out fermentation cultivation on the obtained product for 36-48 h at a temperature of 25-32 DEG C and a pH value of 5.0-7.0 so as to obtain fermentation liquor containing the carotenoids. The Rhodotorula mucilaginosa disclosed by the invention simultaneously has the abilities of carrying out efficient decoloring on triphenlmethane dyes and carrying out high-yield production on carotenoids, therefore, the Rhodotorula mucilaginosa has an extremely broad application prospect in a field to which the Rhodotorula mucilaginosa belongs.

The invention discloses a composite stichopus japonicus growth promotion micro-ecological preparation. The micro-ecological preparation is characterized by comprising photosynthetic bacteria, bacillus subtilis, lactic acid bacteria and rhodotorula benthica, wherein the concentration ratio of the photosynthetic bacteria to the bacillus subtilis to the lactic acid bacteria to the rhodotorula benthica is (3-5.5):(3.5-5):(0.5-1.5):(1-1.5); the final concentration of both the photosynthetic bacteria and the bacillus subtilis is (0.5-1.5)*10<4>cfu / ml; and the final concentration of both the lactic acid bacteria and the rhodotorula benthica is (2-4)*10<3>cfu / ml. When in use, 7-10 days are used as one cycle, the four micro-ecological preparations with the above concentration ratio are diluted by 10-15 times by water, are mixed uniformly and then are uniformly spilled into culture water respectively, in the second day of the primary feeding operation, the preparations are spilled with the amount that is a half of the feeding amount of the first day, and the proper water temperature for feeding is 15DEG C to 28 DEG C. The micro-ecological preparation for the healthy culture of stichopus japonicus is easy to get, significant in effect and relatively low in cost.

The invention provides a method for preparing a compound microorganism fermented forage feed. The method is characterized by firstly preparing a compound microorganism fermentation agent lyophilized powder of trichoderma koningii CICC 13037, rhodotorula mucilaginosa CICC 31192, bacillus subtilis CICC 10063 and bacillus subtilis CICC 10089, activating the lyophilized powder with 2% of glucose solution and then inoculating the activated lyophilized powder into the fresh forage section, then carrying out insulation fermentation and aeration-drying on the forage and then packaging the forage, thus obtaining the finished product.

The invention relates to a method for producing coenzyme Q10 by using rhodotorula gracilis, which belongs to the field of microorganism. The method comprises the following steps: culturing bacteria on a flat plate; preserving the bacteria at minus 80 DEG C after being washed by sterile water; and putting the activated bacteria into a fermentation culture medium to culture. The invention provides new high-potential candidate bacteria for fermenting and producing coenzyme Q10.

The invention discloses a rhodotorula cell and a method for producing optical active alcohols by using the cell. The rhodotorula cell taken as a catalyst can convert substrates of various prochiral ketones under mild reaction condition to obtain corresponding optical active alcohols. The method has high conversion rate of the substrates, high optical purity of products (the enantiomeric excess value is all over 90 percent), and high actual application value and development prospect.

The invention relates to an organic material decomposition agent and a preparation method thereof. The organic material decomposition agent is prepared from the following components in parts by weight: 8-12 parts of strain A and 0.5-2 parts of strain B, wherein the strain A is prepared from the following components in parts by weight: 40-55 parts of bacillus subtilis, 25-35 parts of lactic acid bacteria, 7-13 parts of flavobacteria, 1-5 parts of rhizopus oryzae and 3-8 parts of aspergillus niger; the strain B is prepared from the following components in parts by weight: 0.5-1.5 parts of pichia pastoris and 1-1.5 parts of rhodotorula. The organic material decomposition agent contains various beneficial microorganisms which are matched with each other and are strong in symbiosis; the organic material decomposition agent pays more attention to substance transformation while effectively decomposing organisms, not only can accelerate the decomposition of non-degradable substances such as pectin, cellulose, lignin and the like, but also can ensure the contents of nitrogen, phosphorus and potassium in an organic fertilizer, and furthermore, the composting effect is improved and the quality of a composted finished product is improved; in addition, the organic material decomposition agent has the advantages of low price, wide application range, strong practicability and convenience in popularization.

The invention discloses a butachlor degradation strain and application thereof. The butachlor degradation strain is rhodotorula mucilaginose Y-LCY-1 and is preserved in China General Microbiological Culture Collection Center on September 14, 2009 with a preservation number of GMCCNo.3270. The strain can maximally tolerate 1,000 mg.L<-1> butachlor; and after being cultured in an inorganic salt basal medium containing 100 mg.L<-1> butachlor for 7 days at 25 DEG C with the inoculum concentration of 5 percent and pH of 6.5, the strain can degrade 97.6 percent of the butachlor and has good degradation effect on the butachlor of 50 to 500 mg.L<-1>. The strain provided by the invention can be applied to the biological purification of water bodies and soil polluted by the butachlor.

A spray process for drying solid marine rhodotorule features use of continuous stream plus deep aeration culture and spray drying. Its resultant has high concentration of rhodotorula cells (10-100 billions / g) and high output. It can be used to raise prawn and marin crab for higher surviral rate, health level and raising efficiency.

The invention provides a salt-tolerant rhodotorula mucilaginosa strain with high yield of phenylethanol and application thereof. The strain was deposited in the China General Microbiological Culture Collection Center on November 26, 2019 under a deposit number of CGMCC No. 19008. The strain has characteristics of salt tolerance, better growth stability, and producing strong sauce and mellow flavor, significantly increases content of active ingredients in volatile substances such as phenylethyl alcohol, furanone, maltol, 2,6-dimethylpyrazine, etc., and can be used for fermentation of high-saltdilute soy sauce to improve the flavor and quality of soy sauce products.

The invention relates to a wall breaking method for red yeast. The wall breaking method comprises the following steps: preparing hydrochloric acid with different concentration gradients, performing treatment on the red yeast by an acid-heating method, extracting pigments by an organic solvent and comparing a wall breaking effect. The concentration of the hydrochloric acid used by the wall breaking method for the red yeast, provided by the invention, is low, the wall breaking time is short, the efficiency is high, and the extraction rate of carotenoids is improved by about 40% in comparison with the treatment with the acid in other concentrations; and furthermore, the energy consumption is low, the process is simple, the requirements on equipment are low, the pollution of the carotenoids is reduced by treatment with the hydrochloric acid in low concentration, and the method has very strong practical value and development prospect. The wall breaking method is conductive to improving the yield and the quality of the carotenoids produced by fermentation of the red yeast and reducing the production cost.

The invention relates to the technical field of microorganisms and particularly relates to a bacillus amyloliquefaciens. The bacillus amyloliquefaciens is separated and bred from soil of Jiangxi Fuzhou domestic refuse landfill site; the strain number is SQ-001; the Latin name is bacillus amyloliquefaciens; the strain SQ-001 is preserved in China General Microbiological Culture Collection Center (CGMCC) on August 30, 2016; the preservation address is Building 3, Yard 1, West Beichen Street, Chaoyang District, Beijing; the postcode is 100101; the strain preservation number is CGMCC No.12917. The bacillus amyloliquefaciens strain SQ-001, rhodotorula mucilaginosa CGMCC No. 2.4008, enterococcus faecalis CGMCC No. 1.2024 and acetobacter pasteurianus CGMCC No. 1.2830 are prepared into a composite microbial deodorant through processes of purebred amplification culture and mixed fermentation; the composite microbial deodorant is excellent in deodorization effect and wide in application range.

The invention discloses a special compound feed for breeding parent shrimps of Litopenaeus vannamei. The special compound feed contains the following components in percentage by weight: 20%-45% of whole squid meal, 5%-20% of Peru fish meal, 5%-15% of copepod dry powder, 2%-5% of enzymolysis fish soluble meal, 2%-8% of spirulina powder, 3%-10% of fly maggot meal, 5%-20% of oyster powder, 1%-4% of enzymolysis beer yeast powder, 1%-4% of sea rhodotorula, 5%-20% of alpha-starch, 1%-3% of soybean lecithin, 1%-5% of squid liver oil, 0.5%-2% of calcium dihydrogen phosphate and 1%-3% of a compound premix. The special compound feed is specially used during the breeding of the parent shrimps of Litopenaeus vannamei and is used for supplying nutrients required by the parent shrimps in the breeding stage; by adding multiple components which can mutually generate synergistic interaction effects, the digestion and utilization of the main nutrients are promoted, the immune capacity and health condition of prawns can be improved, the gonadal maturation quality is improved, and the hatching rate of fertilized eggs and the survival rate of larvae are increased.

The present invention relates to the field of fungal biotechnology, more particularly to a strong inducible gene expression system in fungal species, such as a species of the Rhodosporidium genus or the Rhodotorula genus.

The invention discloses a granularity-adjustable laying hen feed and a preparation method thereof and belongs to the technical field of feed processing. The preparation method of the laying hen feed mainly comprises the following steps: taking corns as the main raw materials of the laying hen feed; grinding the corns by a double-roller grinder; screening the grinded corns by a vibration sieve; further grinding the screen residues by a hammer grinder; uniformly mixing the further grinded screen residues with the screened grinded corns; grinding soybean meal, DDGS, corn bran, double-low vegetable meal, monosodium glutamate protein and palm meal by a hammer grinder; then preparing the laying hen feed according to the conventional method. Preferably, the ratio of the digestible amino acids in the laying hen feed is DLys: (DM plus C): DThr: DVal: DArg equals 100: 84: 67: 87: 94; moreover, the yeast powder fermented from the preserved bacteria Rhodotorula benthica WYN1 whose preservation code is CCTC No: M 2014592 is specially added in the laying hen feed; thus, the grinding time is shortened, the dust is reduced, the production efficiency is improved, the breeding effect is raised and the egg quality is improved.

The invention discloses a composite microecological preparation and an application thereof. The composite microecological preparation comprises saccharomycetes, acid-producing bacteria and bacillus, wherein the viable count ratio of the saccharomycetes to the acid-producing bacteria to the bacillus is (1-3): (1-4): (1-2), the effective viable count of each gram of the composite microecological preparation is greater than or equal to 1010 CFU / g, the saccharomycetes are selected from at least one of saccharomyces cerevisiae, phaffia rhodozyma, rhodotorula mucilaginosa and candida utilis, the acid-producing bacteria are selected from at least one of lactobacillus plantarum, enterococcus faecalis, enterococcus faecium, clostridium butyricum and lactobacillus rhamnosus, and the bacillus is selected from at least one of bacillus subtilis, bacillus licheniformis, bacillus coagulans, bacillus amyloliquefaciens and bacillus megatherium. According to the composite microecological preparation and the application thereof, the intestinal health level of animals can be improved, the growth of the animals can be promoted, the anti-stress capability of the animals can be improved, the immune system of the animals can be activated, and diseases can be reduced.

The invention relates to a method of producing an active peptide powder for aquatic products, livestock and poultry through fermentation of chrysanthemum meal. The method includes the steps of: 1) with the chrysanthemum meal, soybean meal, arginine concentrated bacterial slurry dried powder and corn steep liquor dry powder as raw materials and bacillus subtilis, arginine producing bacteria, rhodotorula benthica and lactobacillus plantarum as initial bacterial strains, respectively performing liquid seed cultivation and fermenting cultivation; 2) uniformly mixing a solid and a liquid according to ratio, placing the mixture on a fermentation bed and feeding sterilized air to perform aerobic solid fermentation cultivation; and 3) drying, crushing and sieving the product to obtain the active peptide powder for aquatic products, livestock and poultry. The active peptide powder has certain source of raw materials for fermentation, wherein the chrysanthemum meal, the arginine concentrated bacterial slurry dried powder and the corn syrup powder are low in cost and can produce a large amount of fermentation products. The active peptide powder is added to feeds for aquaculture and livestock and poultry breeding, thereby improving growth capability, improving product quality and also improving breeding environment. The active peptide powder is a green, environment-friendly and functional feed additive used for the aquatic products, livestock and poultry.