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Solid ocean red yeast preparation as well as preparation method and application thereof

A marine red yeast, solid technology, applied in applications, animal feed, fungi, etc., can solve the problems of low cell density of solid marine red yeast, unfavorable long-term storage and long-distance transportation, and insufficient live bacteria rate, achieving bright color, The effect of increasing the live bacteria rate and cell density and increasing the growth rate

Active Publication Date: 2014-03-05
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fresh marine red yeast ecological bait is liquid, and the product has short shelf life and needs to be refrigerated, which is not conducive to long-term storage and long-distance transportation.
Patent Publication No. CN1415738A, the patent on May 7, 2003 is a patent "a starch adsorption fluidized bed drying process for solid marine red yeast"; Patent Publication No. CN1415739A, the patent on May 7, 2003 "A Production Method of Spray Drying of Solid Marine Red Yeast"; Patent Publication No. CN1415740A, the publication date is May 7, 2003 Patent "A Production Method of Bran Adsorption and Drying of Solid Marine Red Yeast", these inventions are described in the specification Disclosed in the culture method of liquid deep-layer aeration continuous fed-batch feeding of marine red yeast, spray drying or carrier adsorption fluidized bed drying production method is adopted for post-treatment, the cell density and viable bacteria rate of the solid marine red yeast product produced by these methods are 50% and 50% respectively. ~10 billion / gram, 20~30%, but there are also the following disadvantages: the solid marine red yeast product has low cell density, and the viable bacteria rate is not high enough

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The solid marine red yeast preparation in this example is mainly made of compound protective agent and concentrated marine red yeast bacterium slime, the mass ratio of the two is 1.5:1, and the cell density of the solid marine red yeast preparation is 3000~ 400 billion / g, the cell activity is 70~80%, the protective agent includes skimmed milk powder, sucrose and water, of which 5% skimmed milk powder (mass percentage, the same below), 10% sucrose (mass percentage, the same below) and the balance is water.

[0042] Wherein the concentrated marine rhodotorula spore is prepared by the following method:

[0043] (1) Test tube activation culture: under aseptic conditions, inoculate Rhodotorula japonicus strains on the slope of the test tube with sterilized seed culture solution, and activate the culture at 25°C for 48 hours;

[0044] Among them, the marine rhodotorula strain used in the present invention was isolated and preserved from seawater fish by the laboratory staff...

Embodiment 2

[0081] The solid marine red yeast preparation in this example is a powder, and it is mainly made of compound protective agent and concentrated marine red yeast fungus slime, the mass ratio of the two is 2.2:1, the cell density of the marine red yeast fungus powder 200-300 billion / g, cell viability 70-80%, protective agent includes skimmed milk powder, sucrose and water, skimmed milk powder 5% (mass percentage, the same below), sucrose 10% (mass percentage, the same below) same) and the balance is water.

[0082] Wherein the concentrated marine rhodotorula spore is prepared by the following method:

[0083] (1) Test tube activation culture: under aseptic conditions, inoculate Rhodotorula japonicus strains (same source as in Example 1) on a test tube slope with sterilized seed medium, and activate and cultivate at 28°C for 48 hours;

[0084] (2) Shake flask expansion culture: under aseptic conditions, use an inoculation loop to pick 2~3 rings of activated strains and inoculate...

Embodiment 3

[0099] The solid marine red yeast preparation in this embodiment is a powder, and it is mainly made of compound protective agent and concentrated marine red yeast fungus slime, the mass ratio of the two is 1.8:1, and the cell density of marine red yeast fungus powder is 300-400 billion / g, the cell activity is 70-80%, the protective agent includes skim milk powder, sucrose and water, skim milk powder 5% (mass percentage, the same below), sucrose 10% (mass percentage, the same below ), and the balance is water.

[0100] Wherein the concentrated marine rhodotorula spore is prepared by the following method:

[0101] (1) Activation and subculture: Rhodotorula colloides is used as the strain (from the intestine of wild shrimp, the isolation process is the same as in Example 1), the seed culture solution is used as the medium, the strain is activated and passed on a slant, and the culture temperature is 24~ 30°C, culture time is 36-48 hours;

[0102] (2) Expanded cultivation in sha...

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Abstract

The invention discloses a solid ocean red yeast preparation which is mainly prepared from a composite protective agent and ocean red yeast bacterial sludge according to a mass ratio of (1.5-2.2):1, wherein the cell density of ocean red yeast in the solid ocean red yeast preparation is 200-400 billion / gram, the cell activity is 70-80%, and the protective agent comprises skim milk powder and cane sugar. The invention further discloses a preparation method of the solid ocean red yeast preparation, The preparation method comprises the following steps: firstly, performing high-density cultivation on the ocean red yeast in a cultivation mode of continuous liquid flow layer ventilation, and further drying by using a vacuum freezing drying method. According to the solid ocean red yeast preparation, the survival rate of the ocean red yeast can be increased to be 70-80%, the cell density in the preparation can be 200-400 billion / gram, and the preparation can be also used in aquatic product feeds as an additive as a product of the preparation is in a solid manner, long in expiration date and convenient to transport.

Description

technical field [0001] The invention relates to a solid marine red yeast preparation and its preparation method and application. Background technique [0002] Marine Rhodotorula is a single-celled eukaryotic organism that exists in the natural marine environment. It is rich in protein, glycogen, unsaturated fatty acids, animal larval growth hormone, and carotenoids mainly astaxanthin. Significantly increase the survival rate of cultured animal larvae, improve the effect of adult feeding, and can enhance the immunity of aquatic animals and reduce the amount of antibiotics. It is an excellent additive for ecological farming; in addition, it has good palatability and a cell size between 4 and 6 μm, which is very suitable Instead of traditional bait, single-celled algae are used as opening bait for marine animal larvae and supplementary bait for the entire larval stage. As a biological bait, marine red yeast is widely used in aquatic products such as fish, shrimp, crab, shellfis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16A23K1/18A23K1/16
Inventor 杨莺莺杨铿李卓佳陈永青梁晓华徐创文文国樑
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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