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Rhodotorula mucilaginosa and application in fermentation production of carotenoid and oils

A gum rhodotorula and carotene technology, applied in the field of yeast, can solve the problems of long production cycle and complicated cultivation process, and achieve good application prospects and economic benefits

Inactive Publication Date: 2014-11-05
HENAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although B. trispora has a high pigment output, the cultivation process is complex and the production cycle is long, while the production of carotenoids by Rhodotorula has simple nutritional requirements and a short cultivation cycle. The bacteria are rich in protein, amino acids and vitamins, which can be synthesized Advantages of using

Method used

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  • Rhodotorula mucilaginosa and application in fermentation production of carotenoid and oils
  • Rhodotorula mucilaginosa and application in fermentation production of carotenoid and oils
  • Rhodotorula mucilaginosa and application in fermentation production of carotenoid and oils

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Embodiment 1

[0025]Slant culture and seed culture are carried out on the rhodotorula gluei to obtain activated seed solution; the seed solution is inoculated into the carotenoid-producing fermentation medium according to the inoculation amount of 10% by volume, at 27-30°C, pH4.5 -8.0, carbon-to-nitrogen ratio 25-35:1, 190rpm shaking culture for 96h, the fermented liquid containing carotenoids can be obtained, wherein the carbon source is glucose or sucrose, glucose is the best, and the highest yield of carotenoids can reach 22.93 mg / L, the composition of the carotenoid-producing fermentation medium in g / L is: glucose 31.8, yeast extract 1, ammonium sulfate 2, potassium dihydrogen phosphate 1, anhydrous calcium chloride 0.1, magnesium sulfate heptahydrate 0.5, sodium chloride 0.1, the rest is water, pH7.0. The pigments produced are determined by high performance liquid chromatography, and the main components are carotenoids, such as figure 2 and image 3 shown.

[0026] The strain can p...

Embodiment 2

[0028] Slant culture and seed culture of the Rhodotorula gluei are carried out to obtain activated seed liquid; the seed liquid is inoculated into the oil-producing fermentation medium according to the inoculation amount of 10% by volume, at 26-30°C, pH4.5-8.0 , carbon-nitrogen ratio 30-130:1, 190rpm shaking culture for 96h, the fermented liquid containing oil can be obtained, wherein the carbon source is glucose or sucrose, and the oil can be produced under the condition of glucose and sucrose as the carbon source, and the highest oil produced can reach 27.8%, the composition of the oil-producing fermentation medium is calculated in g / L: glucose 85.6, yeast extract powder 0.5, potassium dihydrogen phosphate 1, ammonium sulfate 2, magnesium sulfate heptahydrate 0.5, and the remaining ingredients are water, pH6. 0, using Soxhlet extraction method for oil extraction, after GC-MS to identify the produced oil, the produced oil is mainly C16 and C18 unsaturated fatty acids, such as ...

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Abstract

The invention discloses a Rhodotorula mucilaginosa strain and application in fermentation production of carotenoid and oils. The main point of the technical scheme involved in the invention lies in that: the Rhodotorula mucilaginosa strain is preserved in China General Microbiological Culture Collection Center with a preservation number of CGMCC No.8926. The Rhodotorula mucilaginosa provided by the invention can be used for fermentation production of carotenoid and oils. According to the invention, Rhodotorula mucilaginosa is utilized to produce carotenoid and oils at the same time, multi-round ARTP (atmospheric and room temperature plasma) mutagenesis is carried out on the production strain so as to obtain a high yield mutant strain, thus providing good application prospects and economic benefits for industrial production of carotenoid and oils.

Description

technical field [0001] The invention belongs to the technical field of yeasts, and in particular relates to a strain of rhodotorula japonicus and its application in the fermentative production of carotenoids and oils. Background technique [0002] Carotenoids are a general term for a group of fat-soluble pigments with antioxidant properties. As a class of important biologically active substances in humans and animals, carotenoids have important functions such as enhancing the body's immunity, anti-radiation, anti-tumor, and treating photosensitivity diseases. , It has a wide range of applications in food, cosmetics, medicine and feed industries. At present, carotenoids have been recognized as Class A nutritional pigments by international organizations such as FAO, European Community and WHO, and in recent years, the industrialization development of carotenoids is gradually taking shape. [0003] The industrial production method of carotenoids can be chemical synthesis or pl...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P23/00C12P7/64C12R1/645
Inventor 杨清香张昊蒿洪欣王瑞飞刘冬董慧霞
Owner HENAN NORMAL UNIV
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