2489results about How to "Shorten the growth cycle" patented technology

A method for breeding stat1a (signal transducer and activator of transcription 1) gene-deleted zebra fish through gene knockout comprises steps as follows: design of a CRISPR / Cas9 gene knockout target site: a gRNA expression carrier is established and gRNA is synthesized in vitro; micro-injection of a zebra fish embryo; detection of effectiveness of the target site with a T7E1 method through Sanger sequencing; tail cutting identification according to the identification steps after two months of injection; TA cloning of a target sequence; Sanger sequencing of plasmids; obtaining of heritable F1 generation of a zebra fish mutant; obtaining of F2 generation homozygote of the zebra fish mutant, F3 generation pure line inheritance of the gene-deleted zebra fish with the above method, and obtaining of a new zebra fish strain.

The invention provides statla gene deletion type zebra fish. After an experiment design region is knocked out, the sequence is represented as SEQ ID No.1; an experiment comprises the following steps: designing a CRISPR / Cas9 gene knockout target point: constructing a gRNA expression vector and synthesizing gRNA in vitro; carrying out micro-injection of zebra fish embryos; detecting the effectiveness of the target point by a T7E1 method and Sanger sequencing; two months after injection, carrying out tail shearing and identification and carrying out identification steps above; carrying out TA cloning of a target sequence; carrying out Sanger sequencing of plasmids; obtaining an F1 generation of descendible zebra fish mutants; obtaining F2 generation homozygotes of the zebra fish mutants; and carrying out F3 generation homozygous heredity of the gene deletion type zebra fish according to the method above to obtain a new zebra fish line.

The invention discloses an intelligent semiconductor illumination system for plant growth and a spectral modulation method of the intelligent semiconductor illumination system for plant growth, aiming to solve the problems that the existing light source for plant growth has too narrow light spectrum during light supplementing, is short of infrared and far-infrared light and emits too strong blue light to cause damage to human eyes easily. The intelligent semiconductor illumination system for plant growth comprises a parameter setting software module, a master control module, a data storage module, a light source driving module, a human body sensing module and the at least one semiconductor light source; the master control module is connected with the parameter setting software module, the light source driving module and the human body sensing module respectively; the master control module is provided with a data analysis software module and a data storage module; the light source driving module is connected with the semiconductor light source; and the semiconductor light source comprises a plurality of white semiconductor light source and a plurality of red semiconductor light source. The system and the method solve the problems on the infrared and far-infrared light deficiency, the damage of blue light of an LED (light-emitting diode) for illumination of plant growth and the economy of the intelligent semiconductor light source illumination system.

An indoor soilless plant cultivating system, comprising a plurality of stationary light posts, each of which adapted to illuminate a predetermined sector of an indoor facility in accordance with a predetermined illumination signature; a plurality of plant growth towers that are rotatable about a substantially vertical axis in accordance with a predetermined timing sequence so as to be exposable to the light generated at any given time by one or more of the light posts and that are arranged by at least one module defining a module darkened interior region within which plants being instantaneously positioned receive a sensation of nighttime; and irrigation means for supplying the plants being cultivated in each of said towers with a nutrient-rich solution. Moreover, an artificial pollination system based on compressed air nozzles is incorporated within the light posts.

The invention discloses ankistrodesmus sp containing lipid as well as culture and applications of ankistrodesmus sp, wherein the ankistrodesmus strain is SS-B7, the classification name of SS-B7 is Ankistrodesmus sp, and the ankistrodesmus sp SS-B7 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on April 15, 2013, and has the preservation number CGMCC No.7478. The ankistrodesmus sp SS-B7 has tolerance to CO2 and NOx with high concentrations, and has high carbon sequestration efficiency, the content of total lipids in the cells of the obtained biomass accounts for 40% or above of the dry cell weight, and the ankistrodesmus sp SS-B7 can be used for producing biodiesel.

The invention provides a Kyropoulos method for quickly growing a large-size sapphire single crystal. Processes of charging, vacuumizing, heating for melting, auxiliary temperature field adjustment, crystal leading, shoulder extending, shoulder contracting, equal-diameter growth, pull-off, cooling annealing and discharging are finished in a growth furnace of the large-size sapphire single crystal. The Kyropoulos method for quickly growing the large-size sapphire single crystal is greatly improved on the basis of the conventional Kyropoulos method, and has the advantages of shorter growth period, larger size and higher quality of the grown crystal, higher yield of the crystal, lower unit production cost and the like.

The invention relates to a quick-acting multi-microorganism composite water flush fertilizer and a preparation method thereof. The quick-acting multi-microorganism composite water flush fertilizer is characterized by being prepared by mixing bacterial powder which is prepared by fermenting and culturing photosynthetic bacteria, pichiafermentanslodder, lactic acid bacteria, bifidobacterium, bacillus subtilis, bacillus licheniformis, brevibacillus laterosporus, bacillus megaterium, bacillus mucilaginosus, azotobocter chroococcum and actinomycetes. The density of the pichiafermentanslodder ACCC20319T in the quick-acting multi-microorganism composite water flush fertilizer is more than or equal to 2.0*10<8>cfu / g, and the densities of other bacteria are more than or equal to 2.0*10<9>cfu / g. The quick-acting multi-microorganism composite water flush fertilizer can improve the quality and yield of crops and shorten growth period of crops.

The invention relates to an energy source plant-mushroom fungus combined renovating technique for heavy metal polluted soil, belonging to the technical field of environmental energy sources. Energy source plants and mushroom fungus are planted in intercrop, interplanting or crop rotation on the polluted soil for enriching heavy metal ions; after materializing processing or biofermentation, the mature plants generate energy source substance to be used in the field of industrial fuel; plant residue and mushroom fungus body are put in a biogas tank with certain proportion to generate biogas; heavy metal ions in the methane liquid can be removed through chemical deposition; and the chemical deposition and biogas residues are intensively filled and buried. The energy source plant enricher in the invention can renovate deep soil, beautify the environment and generate energy source benefits and can be planted in large area, while the mushroom fungus enricher has the advantages of strong heavy metal ion enriching ability, short growth period and mature planting technique; the energy source plant and the mushroom fungus are combined to renovate the heavy metal polluted soil so as to achieve higher renovating efficiency and get energy benefits. 10-20 percent of polluted heavy metal can be removed in the cultivation layer of the soil per year.

Thin freestanding nitride films are used as a growth substrate to enhance the optical, electrical, mechanical and mobility of nitride based devices and to enable the use of thick transparent conductive oxides. Optoelectronic devices such as LEDs, laser diodes, solar cells, biomedical devices, thermoelectrics, and other optoelectronic devices may be fabricated on the freestanding nitride films. The refractive index of the freestanding nitride films can be controlled via alloy composition. Light guiding or light extraction optical elements may be formed based on freestanding nitride films with or without layers. Dual sided processing is enabled by use of these freestanding nitride films. This enables more efficient output for light emitting devices and more efficient energy conversion for solar cells.

The invention discloses a method for planting lentinus edodes by using mulberry branch wood chips as a planting material, mainly comprising the following steps: preparing the planting material; sterilizing the planting material at high temperature; inoculating and managing the output of the lentinus edodes; the method reasonably controls the material mixture ratio of the planting material through science and strictly controls technological conditions, such as temperature, humidity and the like in the planting process, therefore, the planted lentinus edodes has short growth period, high biotransformation rate, high yield, uniform individuals and abundant nutrition and belongs to a functional food of a novel generation.

The invention discloses an artificial cultivation method of hemsley rockvine root. The artificial cultivation method comprises the following steps of: 1) selecting an annual or biennial branch which vigorously grows and is well developed and not damaged by diseases or pests, cutting the branch with the length of 4 nodes, keeping 2 leaves on the upper part of the cutting, shearing a lower cut of the cutting into a smooth diagonal plane, and soaking in 500mg / L rooting powder solution; 2) selecting field sandy soil, obliquely cutting the cutting at an angle of 35 to 45 DEG with the ground with the depth of 2 nodes of a lower part, slightly compacting the surrounding substrate and immediately watering after the cutting is completed, keeping the cutting soil humid and keeping shading and ventilation within 15 days after the cutting; and 3) transplanting the cut seedlings to fields in March and April each year, controlling cultivation density, shading by using a sunshade screen in the cultivation period, and applying a calcium fertilizer after 15 days of transplantation. By the technical scheme, the hemsley rockvine root is successfully bred and cultivated, so that a wild hemsley rockvine root resource is protected, artificial excessive collection and digging are avoided, and the requirement of clinical and scientific research medication can be met.

The invention discloses a preparation method of a cadmium selenide or cadmium sulfide two-dimensional monocrystal nanosheet. The method comprises the steps that the CdSe or CdS two-dimensional monocrystal nanosheet is prepared through a van der Waals epitaxial growth technology, the method is characterized in that a mica sheet which is smooth in surface and free of chemical dangling bond is adopted to serve as a substrate, CdCl2 powder or Se powder or S powder serves as a source material, argon serves as carrier gas, CdCl2 stream is reacted with Se or S steam to form CdSe or CdS steam at high temperature, and the steam is deposited on the mica sheet for nucleation and epitaxially grows into the CdSe or CdS two-dimensional monocrystal nanosheet. The preparation method of the cadmium selenide or cadmium sulfide two-dimensional monocrystal nanosheet is easy to operate, low in cost and strong in controllability, the obtained CdSe or CdS has the advantages of being good in size uniformity, high in degree of crystallinity and the like, and important research value and wide application prospect in the fields of solar cells, field effect transistors, photoelectric detectors, photocatalyses and the like are achieved.

The invention discloses a method for promoting plant growth by illumination, in particular to designing a light source product with universal meaning according to the spectrum demanded by plant growth, therefore the method of supplementary illumination of plants in raising seedling of plastic tents and factories is found. The method comprises the following processes: (1) seedling preparation; (2) confirming the absorption spectrum of the plants; (3) testing the photosynthesis under different light qualities; (4) comparing and analyzing the experiments so as to confirm the light absorption of different plants; (5) designing light source equipment; (6) supplementary illumination is carried out to the plants. In the method, spectrum test equipment is used for testing the plants with different types; the light sources with different wavelengths are chosen and made according to the absorption spectrum of the plants, therefore the pertinence and efficiency of the light source can be improved efficiently. The bud ratio and the survival ratio can also be improved efficiently, the seedling state can be shortened, the biomass of seedlings can be increased, and the height of the seedlings and the number of leaves are promoted for increasing, thus shortening the growth period of the plants efficiently and improving the efficiency and benefit during raising seedling and growth.

The invention discloses a straight-pull eight-inch silicon single crystal thermal field and a production method of an eight-inch silicon single crystal. The straight-pull eight-inch silicon single crystal thermal field is characterized in that a protective tray compressed sheet is arranged on a lower protective tray compressed sheet; a graphite felt is filled between the lower protective tray compressed sheet and the protective tray compressed sheet; the bottom of a main thermal insulation cylinder is clamped with a lower thermal insulation cylinder; the bottom of the lower thermal insulation cylinder is clamped with the lower protective tray compressed sheet; the bottom of the lower protective tray compressed sheet is clamped with a furnace bottom support ring; an outer guide cylinder is arranged on a guide cylinder support ring; and an inner guide cylinder is clamped on the outer guide cylinder, and a graphite felt is filled between the inner guide cylinder and the outer guide cylinder. The thermal field has compact structure and better thermal insulation property; and in the thermal field, the equal-diameter average pulling speed is improved, equal-diameter power consumption is reduced, production cost is reduced, and production efficiency is improved. The production method of the eight-inch silicon single crystal comprises: clearing and charging a single-crystal furnace, vacuumizing, smelting a material, thermally sealing, automatically seeding, shouldering, carrying out equal-diameter growing and ending. By utilizing the method, the production efficiency and yield of a product are improved.

The invention discloses a process for growing phi 8'' solar-grade Czochralski silicon monocrystalline by using an 18-inch thermal field. By adopting the 18-inch thermal-shielding thermal field and an upper-shaft magnetic fluid sealing device with a cooling system and combining the optimized parameter conditions of crystal-pulling processes, the invention enables the thermal field to have good heat preservation and obviously reduced energy consumption, enables gas to smoothly flow to be beneficial for the exhaustion of volatile matter, stabilizes crystallization, prolongs the service life of equipment and reduces the production cost; and in addition, the invention reduces magnet demagnetization and the evaporation of a magnetic fluid to avoid the air leakage and the slag dropping of the sealing of the magnetic fluid affecting the growth of the Czochralski silicon monocrystalline, improves the sealing performance and prolongs the service life, thereby ensuring the crystal-pulling processes of crystal leading, diameter equalization, shoulder rotation and the like to be stable and improving production efficiency and yield.

A rapid propagation method for tube seedlings of high-grade polygonatum cyrtonema hua relates to the tissue culture technology of plants. The formulas of culture mediums are as follows: an induction culture medium includes MS, 1.0 mg / L of 6-BA, and 0.5 mg / L of NAA, a proliferation culture medium for root tubers with buds include MS, 10 mg / L of 6-BA, 0.5 mg / L of NAA, and 60 g / L of sugar, and a proliferation culture medium for the bud body parts include MS, 1.0 mg / L of 6-BA, 0.5 mg / L of 2,4-D, and 60 g / L of sugar, a proliferation and seedling strengthening culture medium for the root tuber parts include MS, 1.0 mg / L of 6-BA, 0.5 mg / L of NAA, 0 to 50 mg / L of paclobutrazol, and 60 g / L of sugar, and a rooting culture medium for the root tuber parts include MS, 0.5 mg / L of 6-BA, 0.5 mg / L of NAA, and 0.5% of activated charcoal. The method comprises the steps as follows: the surface layers of the root tuber parts of the disinfected root tubers with buds are removed, the root tubers with buds are cut into root tubers and buds after being cultivated in the induction culture medium and the proliferation culture medium, the root tubers are transferred to enrichment culture and cultivated circularly for 3 to 6 generations, the buds are transferred to proliferation culture of the bud parts and are proliferated in a compound and circulating manner for 3 to 6 generations, and proliferation strong seedling culture and rooting culture are performed to final end products which are the root tubers with buds. The method is applicable to rapid propagation and scale production.

The present invention provides a method for integrating large tree by using monomer trees. Said method includes the following steps: firstly, selecting at least two co-generic monomer trees, respectively shaping every monomer tree, removing bark of trunk portion to be connected to expose cambium layer, joining cambium layers between monomer trees, fixing and disinfecting, sealing joined position by using film to obtain integrated large tree, again cultivating said integrated large tree for 3-5 years by adopting conventional cultivation method so as to obtain the invented integrated large tree.

Bean sprouts production method comprises the following process: A. site selection; B. site disinfection; C. seedling tray disinfection; D. seed selection; E. seed soaking; F. get-on carrige management. The present invention advantage is to use the achievement of biotechnology to break through the traditional production mode for in vivo sprouts, and use simple equipment firstly to process standardization aggregate production new mode for novel semi-closed multi-layer dimensioned soilless culture under the worse condition. Bean sprouts production procession is not affected by outside environment, and no fertilizer, hormone and pesticide is used, it is nuisanceless vegetable and belongs to green food. The present invention is short growth cycle, early benefiting, high yield and land saved, and can be factory and intensive production.

The invention provides a formula of a culture medium for industrial production of sparasis crispa and a production process. The formula of the culture medium comprises the following components in percentage by dry weight: 65 to 75 percent of pine sawdust, 10 to 20 percent of husk, 5 to 10 percent of potato powder, 5 to 10 percent of flour, 0.1 to 0.5 percent of peptone, 0.1 percent of ammonium sulfate and 1.2 to 1.8 percent of brown sugar. The production process comprises the following steps of: preparing and bagging the culture medium; sterilizing and inoculating; culturing hypha; inducing a primodium; differentiating the primodium; opening the bag; managing balls; and harvesting. The invention provides a formula of a culture medium for industrial production of sparasis crispa, which has the advantages of rich nutrition, balanced proportion, good ventilation, and capacity of meeting the requirement of industrial production. The production process has the advantages of short hypha culturing time, uniform fruiting at bag openings, good fruiting consistency, convenience of management, contribution to industrial culture, white mushroom body, high yield, and high biological transformation rate of over 43 percent.

The invention relates to a multi-hole foam glass carrier for a fast mass transfer biological fluidized bed and a preparation method and application thereof, which belong to sewage disposal technology. The multi-hole foam glass carrier is prepared by using waste glass as a raw material to be composited with volcanic rocks, coal ash, coal gangue, furnace clinker, iron powder, aluminum powder, binding agents, foaming agents, foam stabilizer and fluxing agents by weight, and multi-hole foam glass is obtained by stages of preheating, fast sintering, foaming, fast cooling and annealing. The preparation process of the multi-hole foam glass carrier is simple in route, easy to control and low in cost, and the prepared carrier is stable in performance, high in porosity, large in specific surface area, resisting in impact, good in mechanical performance, high in biocompatibility, favorable to efficient fixing of the microorganism, and suitable to the fast mass transfer biological fluidized bed. The multi-hole foam glass carrier solves the problem that biological immobilization carriers in the prior art are easy to abrade, small in bio-film formation amount, low in mass transfer effects and the like in the fast mass transfer biological fluidized bed. The preparation method opens up new approaches for preparing the biological carriers, and fills blank spaces in using of foam glass materials in sewage disposal.

The invention discloses an automatic control device for a plant growth environment. The automatic control device comprises a mixing chamber, a growth chamber and a clean air feeder which is connected with the mixing chamber; the bottom part of the mixing chamber is provided with two circulating fans; a first fan is used for eliminating exhaust gas in the mixing chamber and performing thermoregulating and conditioning on air in the mixing chamber; a second fan is used for air current circulation between the mixing chamber and the growth chamber; a plurality of cultivating racks are arranged in the growth chamber; each cultivating rack is divided into multiple layers; each layer is provided with drainage channel and a certain number of LED (light emitting diode) lamps; the drainage channels are connected with a water supply and drainage system and a nutrient solution supply system; growth environment parameter acquisition sensors are also arranged in the growth chamber; data acquired by each sensor are transmitted to a controller through the Internet of Things; various parameters of the plant growth environment are adjusted by the controller according to growth environment curves of plants in different periods; the controller is connected to a man-machine operation interface. According to the automatic control device disclosed by the invention, the seedling and growth cycles of crops can be greatly shortened, the land use rate is increased, and output per unit area is increased.

The invention provides a preparation method of a microalga-based biochar material for heavy metal ion adsorption. The preparation method comprises the following steps that 1, microalgae and water are added into a hydrothermal reaction kettle to prepare microalga suspension liquid; 2, organic acid is added into the microalga suspension liquid, and after the hydrothermal reaction kettle is sealed, protective gas is introduced into the hydrothermal reaction kettle to replace air in the hydrothermal reaction kettle; 3, the hydrothermal reaction kettle in the step 2 is placed in a drying oven for a carbonation reaction, and the microalga-based biochar material is obtained; 4, activating treatment is performed on the microalga-based biochar material, and the activated microalga-based biochar material is obtained. According to the preparation method, biochar is prepared through hydrothermal carbonization by taking the nitrogen-containing microalgae as the raw material, and then activated modification is performed on the biochar through a KOH activating method and a NH3 activating method separately by taking the biochar as a precursor; the activated microalga-based biochar material obtained through activated modification has micro-mesoporous structures, the large specific surface area and the large pore volume, and the adsorption capacity of porous charcoal is greatly improved.

The invention discloses an artificial culture method and a culture medium for fruiting bodies of cordyceps militaris. Based on the culture medium in a 500mL culture bottle, the culture medium comprises the following components: 20 to 25g of cottonseed hull and 120 to 140mL of nutrient solution, wherein the nutrient solution comprises the following components in part by weight: 2.0 to 3.0 parts of wheat flour, 1.0 to 1.5 parts of soya flour, 3.0 to 4.0 parts of white sugar, 0.1 to 0.2 part of magnesium sulfate, 0.1 to 0.2 part of potassium dihydrogen phosphate, 0.05 to 0.1 part of zinc sulfate, 0.05 to 0.1 part of sodium chloride and 95 to 105 parts of water, and the pH value of the nutrient solution is 6 to 8. The artificial culture method comprises the following steps of: preparation of the culture medium, sub-packaging, sterilization, cooling, inoculation, culture and harvesting of fruiting bodies. The invention has the advantages of simple operation, high efficiency, energy conservation, short culture period, high yield of fruiting bodies, and no pollution or solid wastes.

The invention provides a rapid rhizoma polygonati propagation technology method. The method comprises the following steps: breaking seed dormancy by changing temperature treatment and hormone treatment, performing induction in vitro to culture callus by utilizing seeds subjected to dormancy breakage as materials, performing differentiation culture and root induction, and performing vegetative propagation on rhizoma polygonati seedlings. By adoption of the technology, the rhizoma polygonati propagation factor can be greatly improved, the production period is shortened, the sowing quantity is reduced, and the production cost is reduced.

The invention discloses a three-layer co-extruded high-heat-preservation light-conversion PO film, and belongs to the field of agricultural greenhouse films. According to the invention, on the basis of a previous product, ethylene-vinyl acetate copolymer (EVA) is added into metallocene polyethylene (mPE), a light-conversion master batch is added into a middle layer, the formulas of an outer layer,the middle layer and an inner layer are different, the formulas of the outer layer, the middle layer and the inner layer are mixed uniformly respectively, master batches are prepared by using a two-thread worm double-screw extruder, a three-layer film extrusion is formed through a co-extrusion die head to prepare a PO agricultural greenhouse film, and water-based dripping fog-eliminating coatingliquid is coated outside the PO film. The film is anti-aging and free of fog drops, and excellent in puncture resistance and has a service life of 3 years or more. After inspection and testing by an authoritative department, all quality and technical indexes reach or exceed a national standard, some of which are more than twice as good as the national standard. The PO film is good in light-conversion effect, has a light conversion rate reaching 13% or more, is very beneficial to the growth of crops and shortens the growth period of the crops at the same time.

The invention is a virus removal and rapid propagation technology of sweet potato variety 'Shangshu 19', comprising the following steps: selecting plants which are robust without diseases and insect pests and have characteristics of the variety from growing plants; clipping stem with stem tip; removing visible leaves; sterilizing; peeling the stem tip with 1-2 leaf primodia via a dissecting needle; quickly inoculating into a differentiation and induction culture medium; trimming the sterilized seedling into stem segments with 1-2 internode(s) and switching into an enrichment medium after virus detection; selecting sterilized seedling in vigorous growth to cut into small segments with 1-2 internode(s) and switch into a rooting medium; carrying out domestication on seedling after rooting. The invention has stem tip in virus removal and stem segment in rapid propagation, solves the problems of severe virus disease for sweet potato, poor quality of new cultured breed, germplasm degradation, low yield and the like propagated by using sweet potato block and stem. The invention can artificially control the growing conditions, can not be influenced by nature, and has the advantages of small amount of material use, economical culturing materials, short growth cycle, large propagation coefficient and convenient management and is favor of industrial production.

The invention discloses an SnSe-based thermoelectric material and a preparation method thereof. The preparation method includes steps: S100 weighing reaction raw material according to the stoichiometric ratio of the SnSe-based thermoelectric material; S200 refining the reaction raw material through a melt-refining process to obtain SnSe-based thermoelectric material ingots; and S300 placing SnSe-based thermoelectric material ingots obtained from the S200 in a zone smelting furnace and growing polycrystalline SnSe-based thermoelectric material through a zone melting method. The SnSe-based thermoelectric material obtained through the method is preferential and has better thermoelectric performance. Compared with a method for preparing monocrystalline SnSe-based thermoelectric material, the method is simple and is short in growth period, low in cost, and suitable for mass industrial production.

The invention provides an ecological breeding method for silky fowls. The method simulates a wild natural growing status and adopts an orchard stocking mode; a game fowl is raised in each fowl crowd to enhance the movement amount of the fowl crowd; natural ingestion of the silky fowls is combined with a pollution-free feed to feed and Chinese herbal medicines are used for preventing diseases. The silky fowls provided by the invention are basically in a wild mode and have good meat quality; the nutrition is abundant and the survival rate is high; the disadvantages that the density of confined silky fowls is great, the movement amount is less, the meat is loose, the fat content is high, the prevalence rate is high, the medicine dosage is great, and the like are greatly improved.