Virus removal and rapid propagation technology of sweet potato variety 'Shangshu 19' and culture material

Abstract

The invention is a virus removal and rapid propagation technology of sweet potato variety 'Shangshu 19', comprising the following steps: selecting plants which are robust without diseases and insect pests and have characteristics of the variety from growing plants; clipping stem with stem tip; removing visible leaves; sterilizing; peeling the stem tip with 1-2 leaf primodia via a dissecting needle; quickly inoculating into a differentiation and induction culture medium; trimming the sterilized seedling into stem segments with 1-2 internode(s) and switching into an enrichment medium after virus detection; selecting sterilized seedling in vigorous growth to cut into small segments with 1-2 internode(s) and switch into a rooting medium; carrying out domestication on seedling after rooting. The invention has stem tip in virus removal and stem segment in rapid propagation, solves the problems of severe virus disease for sweet potato, poor quality of new cultured breed, germplasm degradation, low yield and the like propagated by using sweet potato block and stem. The invention can artificially control the growing conditions, can not be influenced by nature, and has the advantages of small amount of material use, economical culturing materials, short growth cycle, large propagation coefficient and convenient management and is favor of industrial production.

Description

Technical field [0001] The present invention relates to a plant tissue culture detoxification and rapid propagation technology. Specifically, it uses sweet potato stem apex meristems for detoxification, stem asexual tissue culture for rapid propagation, and tissue culture expansion. Nutrient-medium ratio. Background technique [0002] Sweet potato is a kind of food between grain and vegetables. my country's annual output ranks first in the world. It can be produced in large-scale intensive production and can also be cultivated in a small area. Only Shangqiu City and its surrounding areas have planted 1.5 million mu of sweet potatoes all year round. The use of sweet potatoes to process starch, vermicelli and vermicelli after production has become an effective way for farmers to get rid of poverty and become rich. However, due to the long history of sweet potato cultivation in our city and surrounding areas, the sweet potato virus harms quite severely, the incidence rate of fields ...

AI Technical Summary

Problems solved by technology

After the sweet potato is infected with the virus, the variety will degenerate, and the yield will drop by more than 30%. In severe cases, the yield of the field will drop by more than 50%, and some even fail to harvest; the sweet potato infected with the virus has rough skin, smaller tubers, and lower dryness rate and starch content.