Micro-ecological traditional Chinese medicine preparation for enhancing immunity of livestock and poultry, and preparation method thereof
A technology of traditional Chinese medicine microecology and immunity, which is applied to the traditional Chinese medicine microecological preparation for enhancing the immunity of livestock and poultry and its preparation, the composition of traditional Chinese medicine fermented by probiotics and the field of preparation thereof, which can solve the problem of decreased human and animal immunity, Double and endogenous infection, increase in secondary drug dosage, etc., to achieve the effect of promoting the health of livestock and poultry, reducing breeding investment, and maintaining the balance of flora
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Embodiment 1
[0036] Traditional Chinese Medicine: 10 parts of Astragalus, 6 parts of Eucommia, 6 parts of Licorice, 3 parts of Bupleurum, and 5 parts of Eclipta.
[0037] Fermentation process:
[0038] Inoculate Bacillus licheniformis and Bacillus subtilis into ordinary nutrient broth medium respectively, cultivate them on a shaker at 37°C and 200rpm to produce first-grade seed liquid, and when the bacterial content reaches 10 7-8 When the CFU / ml is about 5%, inoculate the first-grade seed solution with 5% inoculation amount into the second-grade seed fermenter equipped with nutrient broth to expand the cultivation. The cultivation temperature is 28-35°C and the rotation speed is 200rpm. 10 9 When the CFU / ml is about, stop the fermentation and set aside;
[0039] Inoculate Saccharomyces cerevisiae into the wort medium, culture on a shaker at 28°C and 200rpm to produce first-grade seed liquid, when the bacterial content reaches 10 7-8 When the CFU / ml is about 6%, inoculate the first-leve...
Embodiment 2
[0043]Traditional Chinese Medicine: 8 parts of Astragalus, 6 parts of Eucommia, 10 parts of Licorice, 3 parts of Bupleurum, 4 parts of Eclipta, 5 parts of Ligustrum lucidum, 3 parts of Salvia, 10 parts of wolfberry, 6 parts of barley, 3 parts of Scrophulariaceae, 2 parts of Fangfeng 6 servings of yam, 5 servings of chive seeds, 3 servings of rehmannia, and 6 servings of dandelion.
[0044] Fermentation process:
[0045] Inoculate Bacillus licheniformis into common nutrient broth medium, culture at 37°C, 200rpm shaker, produce first-grade seed liquid, when the bacterial content reaches 10 7-8 When the CFU / ml is about 3%, inoculate the first-level seed solution with an inoculation amount of 3% into the second-level seed fermenter to expand the culture. The culture temperature is 30-32°C and the rotation speed is 200rpm. 9 When the CFU / ml is about, stop the fermentation and set aside;
[0046] Inoculate Candida utilis into the wort medium, culture at 28°C, 200rpm shaker, produc...
Embodiment 3
[0051] Traditional Chinese Medicine: 5 parts of Astragalus, 8 parts of Eucommia, 10 parts of Licorice, 2 parts of Bupleurum, 2 parts of Ginger, 4 parts of Eclipta, 5 parts of Ligustrum lucidum, 3 parts of Salvia, 9 parts of wolfberry, and 5 parts of Job's tears.
[0052] Fermentation process:
[0053] Inoculate Bacillus licheniformis into common nutrient broth medium, culture at 37°C, 200rpm shaker, produce first-grade seed liquid, when the bacterial content reaches 10 7-8 When the CFU / ml is about 3%, inoculate the first-level seed liquid into the second-level seed fermenter with an inoculation amount of 3% to expand the culture. The culture temperature is 30±2°C and the rotation speed is 200rpm. 9 When the CFU / ml is about, stop the fermentation and set aside;
[0054] Inoculate Candida utilis and Saccharomyces cerevisiae into the wort medium, culture on a shaker at 28°C and 200rpm to produce first-grade seed liquid, when the bacterial content reaches 10 7-8 When the CFU / ml ...
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