The invention relates to a method for preparing
peptide with high
antioxidant activity by utilizing rice residue
protein treated by superhigh pressure. The method comprises the following steps: (1) pretreating rice residues so as to obtain rice residue
powder; (2) extracting the rice residue
protein: mixing the rice residue
powder and water so as to obtain a mixture, regulating the pH value of the mixture to be 11-13, stirring the mixture for a reaction, performing
centrifugation so as to collect supernatant, regulating the pH value of the supernatant to 5.0-5.8, precipitating the supernatant of which the pH value is regulated, centrifuging the precipitated supernatant and washing the centrifuged supernatant so as to obtain the rice residue
protein; (3) treating the rice residue protein through superhigh pressure: treating the rice residue protein under the superhigh pressure, and
freeze drying the treated rice residue protein so as to obtain superhigh-pressure rice residue protein
powder; (4) performing enzymolysis: compounding the superhigh-pressure rice residue protein powder into an
aqueous solution, performing enzymolysis on the
aqueous solution, performing
enzyme deactivation on the queous solution after enzymolysis, centrifuging the queous solution on which the
enzyme deactivation is performed, and collecting supernatant; (5) performing vacuum concentration on the supernatant so as to obtain a concentrated solution; and (6)
freeze drying the concentrated solution so as to obtain the
peptide with high
antioxidant activity. Products obtained by the method disclosed by the invention have higher
antioxidant activity; compared with products which are not treated through the superhigh pressure, the
clearance rate for
DPPH free radicals, the
clearance rate for
ABTS free radicals and the
clearance rate for hydroxyl free radicals in the products obtained by the method disclosed by the invention are greatly increased respectively.