2358results about How to "Improves antioxidant activity" patented technology

An antioxidant composition having enhanced oxidative stability, emulsion stability, and health benefits. The composition may include individual ingredients or a synergistic blend of non-reducing sugars, sugar polyols, medium-chain triglycerides, polysaccharides, polyphenols, phospholipids, chitosan, and alpha-casein, beta-casein, kappa-casein or protein fragments, glycopeptides, phosphopeptides. The composition may optionally be further utilized for the prevention of hypercholesterolemia or bone mineral loss.

The present invention includes an apparatus and method for detecting directly the antioxidant activity of both lipophilic and lipophobic anti-oxidants that uses an oxygen radical sensitive sensor in fluid communication with a sample in a solvent / water / surfactant mixture; wherein the oxygen radical sensitive sensor concurrently detects both lipophilic and lipophobic anti-oxidants in the solvent / water / surfactant mixture.

The present invention relates to compositions useful in treating hyperpigmentation and the various signs of dermatological aging in human skin. The present invention also relates to cosmetic compositions and methods of using such compositions that improve the aesthetic appearance of skin. Further, the present invention relates to methods of applying the compositions to the skin to effect treatment and to improve the aesthetic appearance of skin, particularly, by providing anti-aging benefits to the skin. These and other objects of the present invention are achieved by a method and composition that comprises (a) a de-pigmenting agent or anti-aging agent in an amount effective to prevent, treat and / or ameliorate pigmentation or the various signs of aging at an area of skin to which it is applied, and (b) a cosmetically or pharmaceutically acceptable vehicle. Suitable de-pigmenting agents include 3,3'-thiodipropionic acid, thiazolidine-2-carboxylic acid, Kaempferol-7-glucoside, perilla oil, and clofibrate and clofibrate analogs and / or derivatives, as well as those set forth below. Suitable anti-aging agents include 3,3'-thiodipropionic acid and / or its derivatives.

The preparation methods of globefish skin active collagen and the peptide of the active collagen are that the globefish skin is cut into small pieces to be frozen, dried, crushed and put into sodium chloride solution to be stirred for 12 hours and then for centrifugation to discard supernatant and remove non-collagen proteins, deposition is put in to acetum, pepsin is added under 0 DEG C to 10 DEG C for enzymolysis and centrifugation, the supernatant is put through an ultrafiltration membrane, and the above part is frozen and dried to obtain the collagen. After the active collagen is prepared, the rest deposition is centrifugated until the pH value is between 2.0 to 7.0, papain is added into the deposition for the enzymolysis, the supernatant is centrifugated and respectively put through the ultrafiltration membrane after enzyme-denaturing to obtain the globefish skin collagen peptide of different molecular weight ranges, and the globefish skin collagen peptide are frozen and dried to obtain the light yellow or white powdery globefish skin collagen active peptide which has good antioxidation character and takes effects of eliminating free radical and reducing the senescence degree.

The invention discloses an extraction method of anthocyanin and original anthocyanin components in cranberry fruits, which comprises the following steps: crushing the cranberry fruits, enzymolyzing the crushed fruits with biological enzyme, and lixiviating with water under the condition of the pH value being in the range of 1.0-5.5 to obtain water extracting solution and residues; carrying out alcohol extraction on the residues, concentrating the obtained extracting solution and recovering alcohol to obtain alcohol extraction concentrated solution; and merging the water extracting solution with the alcohol extraction concentrated solution, adsorbing with macroporous absorption resin, washing the macroporous absorption resin with water, analyzing with water-containing alcoholic solution, and concentrating and drying the obtained analytical liquid to obtain a mixture containing the anthocyanin and original anthocyanin components. The extraction method of the invention has the advantages of simple process, strong equipment generality, small investment, high yield, low cost and applicability to large-scale production; and the obtained mixture can be used as a raw material of foods, healthcare products and medicines or an additive.

The invention relates to blood lipid-lowering active fractions of forsythia suspensa and a preparation method and use of the same. The active fractions are ethyl acetate fractions obtained by concentrating an extract which is extracted from forsythia suspensa leaves by acetone and water. The compound of phillygenin prepared by the invention and the active fractions, namely the ethyl acetate fractions, at which the phillygenin is positioned have good antioxidant activity in vitro. In-vivo experiments show that the ethyl acetate fractions and the phillygenin can lower total cholesterols and low-density lipoprotein cholesterin in the blood serum of a mouse with hyperlipidemia and can lower blood lipid level by an oxidative approach (raising superoxide dismutase SOD and lowering malonydialdehyde MDA). In addition, the ethyl acetate fractions have a better effect of reducing the total cholesterols in the blood serum of the mouse than the phillygenin and cassia seed blood lipid-lowering tablets. The ethyl acetate fractions of the forsythia suspensa leaves can be further developed to play a role in the multi- target co-prevention and treatment of hyperlipidemia and other diseases and have a good application prospect.

The invention discloses lactobacillus with high antioxidant activity. The lactobacillus named as lactobacillus plantarum BLCC2-0015 is preserved in China Center for Type Culture Collection on March 18, 2015 with the preservation number of CCTCC NO.M2015126. The lactobacillus with high antioxidant activity has the advantages that the lactobacillus is screened from numerous lactobacillus, the lactobacillus is high in antioxidant activity and good in acid and bile salt tolerance, the lactobacillus powder made by the lactobacillus can be produced into body antioxidant food or health care products which can be directly eaten or mixed with water for drinking, use convenience is achieved, and body immunity can be enhanced.

The invention discloses a preparation method for a chlorella anti-oxidative peptide. The preparation method is as follows: by taking chlorella as material, carrying out wall-breaking treatment on the chlorella to obtain soluble protein by utilizing a dilute alkali swelling-combined ultrasonic-wave process; and hydrolyzing the chlorella protein by a biological enzyme method to obtain the chlorella anti-oxidative peptide with remarkable anti-oxidative effect. The invention further discloses a treatment process combining ultralow temperature treatment, high pressure treatment with dilute alkali swelling and ultrasonic waves, which can improve yield of the water-soluble protein and can further improve anti-oxidative ability of hydrolysate. A product obtained by virtue of the preparation method disclosed by the invention is high in purity and strong in anti-oxidative activity.

The invention relates to an anthocyanidin made from Vaccinium uliginosum L. and separation and purification methods thereof, which belong to the technology field of active component extraction and purification and functional food development. The content of anthocyanidin in Vaccinium uliginosum L. is 29.8-40%, wherein the content of malvidin accounts for 4.68-5.48% of the anthocyanidin; and antioxidant activity of the anthocyanidin is as 7.8-15 times as that of Vc. The preparation method includes pre-treating raw material, cold soaking and concentrating, purifying and concentrating, and freeze dying. The ultrasonic and microwave-assisted cold soaking method is employed for separation, and an AB-8 macroporous resin static-dynamic combined absorption purification method is employed. The invention provides an environmental friendly, high-efficiency and low-cost separation and purification technology of anthocyanidin made from Vaccinium uliginosum L., which maintains product bioactivity such as antioxidant property of product and ideal separation effect, establishes the basis for wide utilization of abundant resource of Vaccinium uliginosum L. and industrialized development promotion of a series of Vaccinium uliginosum L. health food, and embodies great economic benefit and social benefit in deep processing of the Vaccinium uliginosum L.

The disclosed item is a composition comprising catechins and sialic acid to provide an effective prevention and treatment for Helicobacter Pylori infection. This composition inhibits growth of Helicobacter pylori, and can increase the antioxidant activity of gastric mucosal cells through reducing the production of O2.−, H2O2 and NO, and decreasing the expression of inducible nitric oxide synthase (iNOS) in cells. The apoptotic damage of stomach caused by Helicobacter pylori was decreased through up-regulation of autophagy to enhance the defensive ability of gastric epithelial cells toward Helicobacter pylori. Therefore the composition can be used in the treatment or prevention on damages caused by Helicobacter pylori infection.

The invention relates to an extraction method of an active substrate, and in particular relates to a method for extracting an active substance from the sea cucumber processing waste. By taking the viscera of sea cucumber as a raw material, the method is used for preparing refined sea cucumber polysaccharide through the processes of pretreatment, enzymolysis, reflux extraction, microwave extraction, alcohol precipitation, decoloration, centrifugation, salt precipitation, dialysis, refining and the like; the supernate after alcohol precipitation is subjected to the processes of rotary evaporation, extraction degreasing, water saturation n-butyl alcohol extraction, rotary evaporation, macroporous adsorption resin chromatography and the like to obtain refined sea cucumber total saponins; and the aqueous solution after the water saturation n-butyl alcohol extraction is processed by the technologies of decoloration, sterilization and the like to obtain antioxidant active sea cucumber polypeptide. The method provided by the invention turns the waste of sea cucumber viscera into wealth so as to obtain three products including sea cucumber polysaccharide, sea cucumber total saponins and antioxidant active sea cucumber polypeptide.

The invention relates to whey protein antioxidant peptides, a preparation method thereof and application thereof. In the method, whey protein, which is a cheese by-product, and an enzymatic preparation undergo enzymatic hydrolysis to form a whey protein antioxidant peptide zymolyte, the zymolyte is subjected to static or dynamic primary purification by macroporous absorption and separation and purification by gel filtration chromatography, preparative high-performance liquid chromatography and analytical high-performance liquid chromatography to give pure whey protein antioxidant peptide products, and a polypeptide amino acid sequence is measured. The product has high digestion resistance, heat resistance, acid resistance and alkali resistance, high storage stability, low molecular weightand specific amino acid sequence, and can effectively remove free radicals, stop oxidation reaction and reduce the generation of oxidation damages, so the products have a wide application prospect inthe field of food, medicaments, feed and cosmetics.

The invention discloses a preparation method of oligomeric proanthocyanidins. The preparation method comprises the following steps of: adding tea polyphenols to a procyanidine plant raw material or an extract thereof; dissolving the procyanidine plant raw material or the extract into an acid aqueous solution according to percentage concentration by weight; carrying out reaction on a mixture for 0.5-5 h at 40-100 G C; adsorbing the solution after reaction by an adsorbing resin; washing the solution after reaction by a distilled water to remove impurities; eluting the solution after reaction by ethanol water; and concentrating and drying the solution after reaction so as to obtain the oligomeric proanthocyanidins. The preparation method of the oligomeric proanthocyanidins, disclosed by the invention, has the advantages of high oligomeric proanthocyanidin content, low average degree of polymerizatoin, high water solubility, high recovery rate, environment friendliness, high efficiency, simplicity and convenience in operation and low cost, can be applied to the fields, such as foods, healthcare foods, drugs, cosmetics, daily chemicals, animal feeds and the like, and is suitable for large-scale industrial production.

The invention relates to the technical field of biology, and discloses an oyster peptide with a sexual promoting function and a preparation method and application thereof. The method comprises the steps that oyster meat, calcium salt and water are ground to obtain oyster meat paste; enzymolysis is performed on the oyster meat paste, and centrifugation is performed to obtain supernatant after enzymolysis is performed, and an oyster enzymolysis coarse solution is obtained; discoloring and impurity removing are performed on the oyster enzymolysis coarse solution, and an oyster peptide refined solution is obtained; concentrating and spray drying are performed on the oyster peptide refined solution to obtain the oyster peptide. According to the oyster peptide with the sexual promoting function and the preparation method and application thereof, due to the fact that the oyster meat is pretreated with the calcium salt before enzymolysis is performed on the oyster meat, endogenous enzymes of oysters are activated, and enzymic preparations consumed in subsequent enzymolysis are reduced; meanwhile, the specific enzymic preparation composition is adopted to cooperate with the endogenous enzymes of the oysters to perform enzymolysis in two steps, and therefore the oyster peptide with the higher antioxidant activity and the significant sexual promoting function is prepared.

An antioxidant composition having enhanced oxidative stability, emulsion stability, and health benefits. The composition may include individual ingredients or a synergistic blend of non-reducing sugars, sugar polyols, medium-chain triglycerides, polysaccharides, polyphenols, phospholipids, chitosan, and alpha-casein, beta-casein, kappa-casein or protein fragments, glycopeptides, phosphopeptides. The composition may optionally be further utilized for the prevention of hypercholesterolemia or bone mineral loss.

The invention relates to Isopentenyl-oxyl substituted dehydrogenized silybin ether and the preparation method and the application thereof. The compound has the activity of eliminating extracorporeal extracorporeal 1-diphenyl-2-picrylhydrazyl radical, the activity of obviously inhibiting the generation of lipid peroxide induced by radical and the function of powerfully preventing the damage of therat adrenal pheochromocytoma PC12 cells due to the hydrogen peroxide, i.e. the function of preventing the oxidative damage of the PC12 cells simulating the brain nerve cells, thereby playing a positive role in preventing the brain cell tissue oxidation and brain never oxidation and preventing and treating the senile dementia and other neurodegenerative diseases. The pharmacodynamic result shows that 7-site substituted dehydrogenized silybin ether can be expectantly used for preparing the medicament for preventing and treating the neurodegenerative diseases.

The invention relates to a gel preparation for maintaining the activity of cryopreserved cells and a stem cell gel preparation containing stem cells. The gel preparation comprises the following components by the mass percentage: 1-3% of sodium alginate, 1-5% of dimethyl sulfoxide, 1-5% of propylene glycol, 1-5% of epigallocatechin gallate, 2-10% of dextran, 1-5% of human blood albumin, and water or a phosphate solution added to make the total amount be 100%. The gel preparation has the advantages of good biocompatibility and simple operation, can maintain high activity of cells, can be directly used for low temperature preservation, and has no need of traditional liquid nitrogen cryopreservation; the cell gel preparation after resuscitation can still maintain high activity of the cells and dryness of the cells, and can be applied for treatment of skin injury or mucosal injury.

The invention relates to a formula of an aquatic product composite preservative. The formula of the aquatic product composite preservative is characterized in that the aquatic product composite preservative in each liter of distilled water comprises the following components: 0.4 to 0.6 g of muramidase, 2.5 to 3.5 g of tea polyphenol, 16 to 20 g of carboxymethyl chitosan, 10 to 14 g of nisin, 25 to 35 g of potassium sorbate, 17 to 23 g of sodium alginate, 20 to 30 g of lactobacillus, 1.5 to 2.5 g of propolis extracts and 10 to 14 g of spice extracts; and the spice extracting liquid comprises the raw materials: galangal, garlic, onion, cinnamon, clove and rosemary. The aquatic product composite preservative provided by the invention is prepared by compounding biological preservatives, is safe and non-toxic, has simple preparation and application methods, prominent microbial inhibition effect and good preservation effect, can greatly prolong the shelf life of frozen aquatic products, and has important industrial value.

The invention provides an apple facial mask based on a traditional Chinese medicine formula.The apple mask comprises a silk matrix and a water-soluble substance.The water-soluble substance comprises apple extract, traditional Chinese medicine extract, seaweed extract, sodium hyaluronate, beta-glucan and assistant.The traditional Chinese medicine extract comprises tremella extract, centella asiatica extract, polygonum cuspidatum root extract, scutellaria root extract, tea extract, glycyrrhiza glabra root extract, chamomilla recutita flower extract, rosmarinus officinalis leaf extract and purslane herb extract.The seaweed extract contains chondrus crispus and bio-saccharide gum-1.The assistant comprises butanediol, propylene glycol, diglycerol, polyethylene glycol-400, Carbomer, hydroxyethyl cellulose, urea, allantoin, glycine betaine, nicotinamide, sodium glutamate, triethanolamine, 1,2-hexanediol, decoyl hydroximic acid, 1,3-propylene glycol, hexanediol and deionized water.The apple mask has the effects that stability is good, dispersibility is high, nutrients are proper in quantity and balanced, antibiosis and anticorrosion effects are achieved, and moisturizing, skin whitening and stain lightening are achieved.

The invention discloses a fermentation tea juice and a preparation method thereof. The preparation method comprises the following steps: taking tea leaves, crushing, adding water to extract, adding beta-glucosidase to the obtained extract liquid in order to carry out enzymatic hydrolysis, and filtering the above obtained enzymatic hydrolysis liquid to obtain a tea juice; concentrating the tea juice until the concentration of tea polyphenol is 10-120g / L, and adding a carbon source and a plant source oligopeptide into the tea juice in order to disinfect; inoculating Leuconostoc mesenteroides into the tea juice after the disinfection in order to ferment, and inoculating yeast and lactic acid bacteria into the above obtained fermentation liquid when the pH value of the fermentation liquid is 4.0-4.5 in order to carry out mixed fermentation; and collecting the obtained fermentation liquid, and post-processing the fermentation liquid to prepare the fermentation tea juice. In the invention, the glucoside modification of catechin is carried out in the fermentation process, so the flavor quality and the bioavailability of the above tea juice fermentation beverage are improved, the stability of functional components is improved, and the overall coordination effect of bioactive components in the tea leaves are comprehensively performed.

The invention discloses a skin-whitening composition as well as a preparation method and applications of the skin-whitening composition. In the skin-whitening composition, various whitening agents and sun-screening agents with excellent properties are matched for use, so that the melanin formation can be inhibited, moreover, ultraviolet rays can be absorbed, and free radicals can be eliminated. The skin-whitening composition is prepared from radix puerariae extract, glutathione, kojic dipalmitate, ascorbyl glucoside, nicotinamide, beta-hydroxy acid, undecylenoyl phenylalanine, chamomile extract, green tea extract, licorice root extract, pomegranate bark extract, 1,3-butanediol, phenoxyethanol and water. The components are mixed to achieve the synergistic skin whitening effect, the skin whitening effect is obvious, safe and steady, and no toxic and side effects are caused.

The invention relates to a method for extracting anthocyanin from plant berries and particularly relates to a preparation method of acanthopanax sessiliflorus berry anthocyanin with antioxidant activity. The preparation method comprises the following steps of (1) selecting acanthopanax sessiliflorus berries as raw materials; (2) extracting the acanthopanax sessiliflorus berry anthocyanin by using an enzyme freezing and thawing method: mixing the frozen raw materials and an extracting solution, unfreezing and breaking the frozen raw materials, and carrying out enzymolysis; (3) carrying out ultrasonic-microwave synergistic extraction on the acanthopanax sessiliflorus berry anthocyanin: carrying out ultrasonic-microwave synergistic extraction on enzymatic hydrolysate, and centrifuging to obtain a crude extracting solution; (4) purifying the crude extruding solution of the anthocyanin: absorbing the crude extruding solution by using macroporous resin, collecting an eluant, and carrying out vacuum concentration; (5) freezing and drying in vacuum; and (6) smashing the anthocyanin, and then, packaging the anthocyanin. The preparation method has the advantages of high extracting efficiency, short time, low energy consumption, small reagent dosage, stable properties of effective components and the like; and meanwhile, a vacuum freeze drying technology is adopted, so that a product has better biological activity, color, luster and solubility and the preparation method is suitable for industrial production.

The invention discloses a whey protein active peptide with antioxidant activity and a preparation method thereof. The preparation method comprises the following steps: (1) adding protease into whey protein solution for enzymolysis, wherein the enzymolysis temperature is 40-60 DEG C, and the pH value is 6.0-8.0; (2) after enzymolysis, inactivating the protease; (3) precipitating the whey protein not hydrolyzed in enzymolysis solution, and removing the whey protein to take supernatant; (4) carrying out ultrafiltration on the supernatant by an ultrafiltration membrane the cut-off molecular weight of which is less than or equal to 10,000 dalton, collecting filter liquor of peptide the molecular weight of which is less than or equal to 10,000 dalton; (5) concentrating the filter liquor; (6) desalting the concentrated liquor; and (7) concentrating the desalted solution, and then freezing and drying. In the invention, the obtained whey protein active peptide has significant antioxidant property and specific functional characteristics and stable quality, and can be widely applied to the fields of food, cosmetics, medicine and the like. The production technology is simple and is convenientfor scale production, the raw material whey protein has high use ratio, and the production cost is low.

The invention discloses a 4-carbamate-3-methoxy cinnamic acid cyclamine alkyl amide compound, and a preparation method and application thereof. The compound prepared in the invention has low toxic and side effect, and can treat neurodegenerative diseases including, but not limited to, vascular dementia, the Alzheimer's disease, the Parkinson's disease, the Huntington's disease, HIV-associated dementia, multiple sclerosis, amyotrophic lateral sclerosis, neuropathic pain, glaucoma and the like.

The invention discloses an antioxidant peptide of hairtail fish protein as well as a preparation method and uses of the antioxidant peptide. In particular, hairtail fish is used as raw materials; an enzymatic hydrolysis solution is obtained through degreasing and enzymolysis of protamex and neutral protease; the enzymatic hydrolysis solution is separated and purified through ultra-filtration, purification of macroporous resins, cation exchange resin chromatography, gel column chromatography, and reversed-phase high-performance liquid chromatography so as to obtain the antioxidant peptide Asn-Trp-Asp-Met-Glu-Lys-Ile-Trp, and the ESI-MS determining molecular weight is 1121.25da. The high-activity antioxidant peptide disclosed by the invention has a good scavenging effect on DPPH free radicals, hydroxyl radicals and superoxide anion free radicals; besides, the antioxidant peptide shows a good effect of lipid peroxidation inhibition. The antioxidant peptide has the advantages of safe use, zero toxic or side effects, high oxidative activity and the like, is liable to digest and absorb, and can be used as medicines, health food or food additives, and the like.

The invention discloses a method for comprehensively extracting various kinds of biologically active ingredients from mushroom dry powder; the technical proposal is as follows: firstly, extracting alcohol soluble substances; secondly, extracting mushroom proteins; thirdly, extracting water soluble polysaccharide of mushroom, fourthly, extracting alkali extraction water-insoluble polysaccharide and alkali extraction water soluble polysaccharide; fifthly, preparing mushroom fiber. The extracted constituents have different biological activities, for example, the alcohol soluble substances have strong antioxidation activity; the mushroom proteins are rich in essential amino acid; the lentinan has antineoplastic activity; and the mushroom fiber has the physiological function of a dietary fiber. Therefore, the extracted constituents can be used respectively.

The invention discloses walnut lactic acid bacteria fermented beverage and a preparation method of the walnut lactic acid bacteria fermented beverage. The fermented drink is prepared by taking walnut protein as a raw material, and fermenting the walnut protein through lactic acid bacteria. The specific process procedure comprises the following steps: pulping defatted walnut into walnut milk, performing homogeneity, sterilization and cooling, then mixing the walnut milk with lactic acid bacteria for fermentation, wherein the strain adopted by the fermentation is a mixed strain of lactobacillusbulgaricus and streptococcus thermophilus, and is suitable for a pure walnut milk fermentation system through multi-time domestication. The beverage disclosed by the invention is rich in plant protein and lactic acid bacteria, has higher antioxidant activity, is fermented natural protein beverage with the advantages of walnut and lactic acid bacteria fermented food, and can be applied to the field of functional food or nutritional health-care products.

The invention discloses a fresh keeping agent containing essential oil, for fruits, and an application. The fresh keeping agent for fruits is characterized by being compounded from lysozyme, tea polyphenols, nisin, essential oil A, essential oil B, Vc and distilled water in a certain proportion. According to the fresh keeping agent for fruits, disclosed by the invention, the essential oil is optimized to be used as a main component of the fresh keeping agent, so that the fresh keeping agent has excellent effects of bacteriostasis and oxidation resistance and is applied to corrosion resistance and fresh keeping of the fruits, the fresh keeping and storage period of the fruits can be greatly prolonged, the fruits are guaranteed to maintain original quality in the fresh keeping period, and the quality of the fruits is effectively guaranteed. The fresh keeping agent for the fruits, disclosed by the invention is simple in compounding method, and convenient to apply; and the fresh keeping agent is completely compounded from natural components, and harmful chemical substances are not added, so that the fresh keeping agent is safe and environmental-friendly, and has an important industrial value.

The invention relates to a method for extracting and preparing medlar leaf flavone. The method comprises the following steps of: (1) after picking and removing impurities manually, drying medlar leaves indoor to obtain a medlar leaf dry sample; (2) crushing the medlar leaf dry sample and screening the crushed medlar leaf dry sample to obtain medlar powder; (3) leaching the medlar powder by using ethanol to obtain leaching liquor; (4) grinding the leaching liquor by using a colloid grinder to obtain colloidal medlar leaf leaching solution; (5) homogenizing and separating the colloidal medlar leaf leaching solution to obtain liquid supernatant and filter residue; (6) removing impurities from the liquid supernatant by using a ceramic membrane to obtain filtrate; (7) adsorbing the filtrate by using weak-polarity macroporous adsorption resin and eluting the filtrate by using ethanol solution to obtain medlar leaf flavone-containing eluent; and (8) after concentrating and drying the medlar leaf flavone-containing eluent under a reduced pressure in a vacuum, obtaining the medlar leaf flavone powder. The method has low cost and high extraction rate; and the obtained medlar leaf flavone has stable properties, high content, and high biological activity of heart head blood-vessel disease resistance, cancer resistance, oxidation resistance and the like.