The invention belongs to the field of
processing agricultural products, and particularly relates to a preparation method of
dioscorea opposita thunb slime
polysaccharide and
resistant starch. The preparation method of
dioscorea opposita thunb slime
polysaccharide and
resistant starch comprises the following steps: washing and peeling fresh
dioscorea opposita
thumb, protecting color,
slicing, crushing, homogenizing, putting homogenate into a high-pressure pulsed
electric field for extraction, adding water into the treated dioscorea opposita
thumb homogenate and performing high-speed
centrifugation, and centrifuging
starch residues at the lower layer for three times; respectively collecting supernatant and lower-layer
starch residues obtained through repeated
centrifugation; filtering the centrifuged supernatant through a molecularly imprinted membrane and eluting with water, to obtain a dioscorea opposita
thumb slime
polysaccharide solution, concentrating and
drying to obtain dioscoreaopposita thumb slime polysaccharide, adding water into the lower-layer
starch residues and performing membrane
filtration, preparing a starch
emulsion after filtering, performing pressure-heating treatment in an
autoclave and then adding pancreatic
amylase and
pullulanase for enzymolysis, performing
enzyme denaturing and performing
refrigeration and
ageing, hot-
air drying, pulverizing and screening to obtain the dioscorea opposita thumb
resistant starch.