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Antioxidant sensor, methods and compositions

a technology of anti-oxidant sensors and sensors, applied in the field of anti-oxidant sensors, can solve the problems of lack of activity, skewing the results of fluorescence assays, and sample fluorescence, and achieve the effect of effective amounts of anti-oxidants

Inactive Publication Date: 2005-04-28
MANNATECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

Enables rapid, inexpensive, and direct detection of total antioxidant capacity, leading to the development of effective antioxidant compositions that provide optimal health benefits by combining lipophilic and lipophobic antioxidants, with demonstrated increases in antioxidant levels in individuals using the supplement.

Problems solved by technology

Fluorescence from the sample, therefore, can skew the results of an assay based on fluorescence.
Lack of activity from such a combination with mixed tocopherols and saturation problems are absent from the ORAC(o) method since the concentration of sample is more dilute and since the solvent for the sample contains acetone, water, and a detergent for solubilizing all components of a sample.

Method used

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  • Antioxidant sensor, methods and compositions
  • Antioxidant sensor, methods and compositions
  • Antioxidant sensor, methods and compositions

Examples

Experimental program
Comparison scheme
Effect test

example 1

Comparison of Antioxidant Activity Using ORAC(fl) and ORAC(o) Assays

[0104] Ingredients for an antioxidant composition were analyzed for antioxidant activity using a prior art oxygen radical absorption capacity method that measures fluorescence (ORAC(fl)), and using the method of the present invention that measures dissolved oxygen (ORAC(o)). The antioxidant activity of a product is its ability to protect the system from damage caused by peroxyl radicals.

[0105] Prior Art Method of Measuring Antioxidant Activity for Comparison. For the ORAC(fl) assay, the method of Ou et al. (Ou, B., Hampsch-Woodill, M. and Prior, R. L., J. Agric. Food Chem. 2001, 49, 4619-4626) was followed. Differences from the Ou procedure as published included the speed of the orbital shaker (Ou, 400 rpm; herein, 280 rpm), and the centrifuge speed (Ou, 14,000 rpm, 15 min; herein, 3200 rpm, 15 min and the length of the assay (Ou, 30 min; herein, 100 min). Fluorescein, sodium salt, was obtained from Aldrich (Milwa...

example 2

A Synergistic Antioxidant Composition

[0125] According to the dietary supplement of the present invention, five ingredients were combined into an antioxidant composition, each of which is prominent in the diet of long-lived peoples from regions around the world. The present inventors selected ingredients based on a comprehensive search of the diets in areas known for longevity. The present inventors recognized that the diets in these regions were rich in flavonols and tocopherols. It was further recognized by the inventors that certain natural compounds interact favorably with molecules that reactivate their anti-oxidant activity, e.g., vitamin C. In fact, extensive research supports the role of Vitamin C in the regeneration of, e.g., α-tocopherol (Pizzorno and Murray, Textbook of Natural Medicine, 1999, 2nd Ed. New York, Churchill Livingston).

[0126] Based on this research, the inventors combined isolated and purified extracts from natural and other sources that include high percen...

example 3

ORAC(o) Detection of Anti-oxidant Synergy and Potentiation

[0152] To show that the ORAC(o) assay was able to detect both lipophilic and lipophobic anti-oxidant activity, a series of studies were conducted to show the effect of specific combinations of known concentrations of lipophilic and lipophobic anti-oxidant and the potentiation of the same with the addition of, e.g., grape seed extract and green tea extract as compared to grape seed extract and green tea extract alone. FIG. 15 is a graph showing the ORAC(o) values obtained with different ratios of quercetin and mixed tocopherols. FIG. 16 is a graph showing the ORAC(o) values for different ration of grape seed extract (GES) and green tea extract (GTE). After the determination of the maximum ratio for anti-oxidant activity for GSE and GTE, the two were combined with the optimized ratio for quercetin and mixed tocopherols. FIG. 17 is a graph that shows the ORAC(o) values of the combination of the maximum quercetin:mixed tocophero...

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Abstract

The present invention includes an apparatus and method for detecting directly the antioxidant activity of both lipophilic and lipophobic anti-oxidants that uses an oxygen radical sensitive sensor in fluid communication with a sample in a solvent / water / surfactant mixture; wherein the oxygen radical sensitive sensor concurrently detects both lipophilic and lipophobic anti-oxidants in the solvent / water / surfactant mixture.

Description

FIELD OF THE INVENTION [0001] The present invention relates in general to the field of antioxidant sensors, and more particularly, to antioxidant sensors and methods that directly measure both hydrophilic and hydrophobic anti-oxidants. BACKGROUND OF THE INVENTION [0002] This application claims priority to, and is a continuation-in-part of, U.S. patent application Ser. No. 10 / 648,047, filed Aug. 26, 2004. Without limiting the scope of the invention, its background is described in connection with antioxidant sensor technology and methods for detection. [0003] Biological systems have developed antioxidant systems to combat the effects of radicals and other pro-oxidative species. An antioxidant is any substance that significantly delays or prevents oxidation of an oxidizable substrate when present at low concentrations compared to that of the oxidizable substrate. There are enzymes that are antioxidants, such as superoxide dismutase and catalase, which are coded for by many organisms. S...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A23L33/00A61K31/353A61K31/355A61K31/7048A61K36/24A61K36/82A61K36/87
CPCA61K31/353Y10T436/142222A61K31/7048A61K36/24A61K36/82A61K36/87G01N33/1806A61K31/355Y10S514/904A61K2300/00A61P39/06A61K31/352G01F1/64G01N33/68G01N33/82G01N21/17G01N27/49C12Q1/37A61K31/375B01D53/62A61P39/00Y10S435/882Y10S436/903
Inventor MCANALLEY, BILL H.VENNUM, EILEENMCANALLEY, SHAYNE A.KOEPKE, C. MICHAEL
Owner MANNATECH
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