Method for extracting a great variety of biological active ingredients from dry powder of pasania fungus

Abstract

The invention discloses a method for comprehensively extracting various kinds of biologically active ingredients from mushroom dry powder; the technical proposal is as follows: firstly, extracting alcohol soluble substances; secondly, extracting mushroom proteins; thirdly, extracting water soluble polysaccharide of mushroom, fourthly, extracting alkali extraction water-insoluble polysaccharide and alkali extraction water soluble polysaccharide; fifthly, preparing mushroom fiber. The extracted constituents have different biological activities, for example, the alcohol soluble substances have strong antioxidation activity; the mushroom proteins are rich in essential amino acid; the lentinan has antineoplastic activity; and the mushroom fiber has the physiological function of a dietary fiber. Therefore, the extracted constituents can be used respectively.

Description

Technical field: [0001] The invention relates to a method for comprehensively extracting multiple bioactive components from dry mushroom powder. Background technique: [0002] Among the bioactive ingredients contained in shiitake mushrooms, there are many research reports on lentinan at home and abroad, and the development and utilization are relatively in-depth, while the research and development of other bioactive ingredients in shiitake mushrooms is less. Moreover, the existing methods for extracting biologically active components of Lentinus edodes are mainly the extraction of a single component, such as Lentinan and Purine of Lentinus edodes, and there is no report on the comprehensive extraction of multiple biologically active components. Therefore, in the prior art, when extracting and preparing single ingredients such as lentinan, other active ingredients in the mushrooms are discarded in vain, which not only leads to higher extraction costs, but also wastes the reso...

AI Technical Summary

Problems solved by technology

[0002] Among the bioactive ingredients contained in shiitake mushrooms, there are many research reports on lentinan at home and abroad, and the development and utilization are relatively in-depth, while the research and development of other bioactive ingredients in shiitake mushrooms is less

Moreover, the existing methods for extracting biologically active components of Lentinus edodes are mainly the extraction of a single component, such as Lentinan, Lentinan, etc., and there are no reports on the comprehensive extraction of multiple biologically active components.

Therefore, in the prior art, when extracting and preparing single components such as lentinan, other active ingredients in the mushrooms are discarded in vain, which not only leads to higher extraction costs, but also wastes the resources of the mushrooms

For example, as far as the extraction of lentinan is concerned, the hot water extraction method is commonly used at present. This method is simple to operate, but the polysaccharide yield is low, only 3.09-3.64%, and more than 96% of the ingredients in the raw material of lentinus have not been utilized. ;Using compound enzymatic hydrolysis combined with hot water extraction method can increase the yield to 5-7%, but it needs to use more expensive cellulase, protease, etc., the extraction cost increases, and the enzymes that are also biological macromolecules are easy Mixing with polysaccharides reduces the purity of polysaccharides; if ultrasonic or microwave-assisted extraction methods are used, the yield of polysaccharides may be slightly increased, but equipment investment and energy consumption are increased

Therefore, if only a single component such as lentinan is extracted, more than 90% of the ingredients in the raw material of lentinus edodes will not be utilized, which will inevitably lead to an increase in extraction costs and a waste of lentinus edodes resources