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Method for extracting active substance from sea cucumber processing waste

A technology for active substances and wastes, applied in the fields of drug combination, steroids, organic chemistry, etc., can solve the problems of insufficient development and utilization of scraps, low technical level and comprehensive utilization degree, etc., to increase yield, Reasonable design route and good response effect

Active Publication Date: 2013-08-28
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the technical level and comprehensive utilization of sea cucumber processing in China are still low, and the leftovers (such as sea cucumber intestines, gonads, etc.) in the processing process have not been fully developed and utilized.

Method used

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  • Method for extracting active substance from sea cucumber processing waste
  • Method for extracting active substance from sea cucumber processing waste
  • Method for extracting active substance from sea cucumber processing waste

Examples

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Embodiment 1

[0032] Step (1) Thaw 1500g of frozen sea cucumber viscera in running water, mince them with a meat grinder, put them in a 5000mL Erlenmeyer flask, add 1500ml of distilled water, and homogenate;

[0033] Step (2) Use a constant temperature shaking shaker to raise the temperature to 50°C, preheat at a constant temperature for 30 minutes, add 44g of compound protease (Novozymes Biotechnology Co., Ltd.), and enzymolyze the sea cucumber protein of sea cucumber viscera at 140rpm / min. And enzymatically extract the sea cucumber polysaccharides in the viscera of sea cucumbers, enzymatically hydrolyze for 8 hours, and inactivate the enzyme in a boiling water bath for 10 minutes;

[0034] Step (3) Put the enzyme-inactivated sample system in a round-bottomed flask, add ethanol to make the ethanol concentration in the system reach 60%, reflux extraction at 60°C for 4 hours, and place the system under 850W for microwave extraction for 30 minutes after reflux extraction , let stand overnight...

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Abstract

The invention relates to an extraction method of an active substrate, and in particular relates to a method for extracting an active substance from the sea cucumber processing waste. By taking the viscera of sea cucumber as a raw material, the method is used for preparing refined sea cucumber polysaccharide through the processes of pretreatment, enzymolysis, reflux extraction, microwave extraction, alcohol precipitation, decoloration, centrifugation, salt precipitation, dialysis, refining and the like; the supernate after alcohol precipitation is subjected to the processes of rotary evaporation, extraction degreasing, water saturation n-butyl alcohol extraction, rotary evaporation, macroporous adsorption resin chromatography and the like to obtain refined sea cucumber total saponins; and the aqueous solution after the water saturation n-butyl alcohol extraction is processed by the technologies of decoloration, sterilization and the like to obtain antioxidant active sea cucumber polypeptide. The method provided by the invention turns the waste of sea cucumber viscera into wealth so as to obtain three products including sea cucumber polysaccharide, sea cucumber total saponins and antioxidant active sea cucumber polypeptide.

Description

technical field [0001] The invention relates to a method for extracting active substances, in particular to a method for extracting active substances from sea cucumber processing waste. Background technique [0002] Since ancient times, sea cucumbers have been health food and nutritious food. The "Food Materia Medica" of the Ming Dynasty pointed out that sea cucumbers have the health-preserving functions of nourishing vitality, nourishing the internal organs and dispelling deficiency. Foot enemy ginseng, hence the name sea cucumber; taste sweet and salty, tonify the kidney meridian, benefit the essence, eliminate phlegm, relieve urination, strengthen yang, treat impotence, and kill sores and insects." In recent years, Chinese medicine has proposed to use sea cucumber alone or in combination to treat tumors, aplastic anemia and diabetes, and has achieved good results. Sea cucumbers have been widely used in the treatment of common diseases such as hypertension, tuberculosis,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C07J71/00C12P21/06A61P39/06
Inventor 袁坤山王宝杰王雷刘梅蒋克勇
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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