127results about How to "Strong antioxidant activity" patented technology

The invention discloses a whey protein active peptide with antioxidant activity and a preparation method thereof. The preparation method comprises the following steps: (1) adding protease into whey protein solution for enzymolysis, wherein the enzymolysis temperature is 40-60 DEG C, and the pH value is 6.0-8.0; (2) after enzymolysis, inactivating the protease; (3) precipitating the whey protein not hydrolyzed in enzymolysis solution, and removing the whey protein to take supernatant; (4) carrying out ultrafiltration on the supernatant by an ultrafiltration membrane the cut-off molecular weight of which is less than or equal to 10,000 dalton, collecting filter liquor of peptide the molecular weight of which is less than or equal to 10,000 dalton; (5) concentrating the filter liquor; (6) desalting the concentrated liquor; and (7) concentrating the desalted solution, and then freezing and drying. In the invention, the obtained whey protein active peptide has significant antioxidant property and specific functional characteristics and stable quality, and can be widely applied to the fields of food, cosmetics, medicine and the like. The production technology is simple and is convenientfor scale production, the raw material whey protein has high use ratio, and the production cost is low.

The invention discloses a method for extracting dendrobium polysaccharides through subcritical water and belongs to the field of extraction of natural active ingredients. The method comprises the following steps: smashing, degreasing and drying dendrobe raw materials, then placing the processed materials in a subcritical water extracting device, extracting for 1-3 times and for 5-30 min under the conditions that the temperature is 100-160 DEG C, the pressure 0.5-2.0 MPa, and the liquid-solid ratio of subcritical water to dendrobe powder is 15-45 mL / g; collecting an extracting solution, adding absolute ethyl alcohol, performing standing, and performing centrifugal separation to obtain precipitate which is a crude product; washing with absolute ethyl alcohol, acetone and ethyl ether to obtain the dendrobium polysaccharides. According to the invention, the advanced subcritical water extracting technology is adopted to extract the dendrobium polysaccharides, the extracting time is short, the extracting efficiency is high, and the obtained dendrobium polysaccharides has remarkable antioxidant activity. The method is environment-friendly and free of pollution, has low requirements to equipment, and facilitates industrial production.

The invention discloses an integrated processing and preparing method in a production place for wine rhizoma polygonati. The integrated processing and preparing method in the production place for the wine rhizoma polygonati comprises the following steps: taking a fresh rhizoma polygonati medicinal material, removing impurities and non-medicinal parts, and washing; scalding the washed rhizoma polygonati in boiled water for 3 to 10 minutes, then, directly cutting the scalded rhizoma polygonati into thick pieces, and spreading and drying to half-dry; spraying yellow wine on the cut thick rhizoma polygonati pieces, and moistening for 1 to 5 hours; steaming the moistened rhizoma polygonati in a steaming container for 12 hours, braising for 12 hours, spreading for 24 hours at room temperature, and steaming, braising and drying for 2 to 9 times repeatedly to obtain finished wine rhizoma polygonati. The process disclosed by the invention integrates processing in the production place and a wine rhizoma polygonati process; the produced wine rhizoma polygonati is stable and uniform in quality; the production period can be effectively shortened; the energy consumption and human cost can be reduced; continuous production can be realized; the integrated processing and preparing method in the production place for the wine rhizoma polygonati can be applied to industrial large-scale production well, and meanwhile, the antioxidant activity of the wine rhizoma polygonati which is prepared according to the method disclosed by the invention is remarkably better than commercially available rhizoma polygonati.

The invention discloses an earthworm extract having a skin aging resisting effect and a preparation method and application thereof. The earthworm extract contains polypeptides of which the molecular weight is less than or equal to 30000 Dalton, amino acid, proteins, lumbricin, vitamins and trace elements. The method comprises the following steps: smashing clean earthworms or smashing dry earthworms and soaking into water to obtain earthworm sample liquid; hydrolyzing the earthworm sample liquid by using own enzyme or added protease to obtain earthworm hydrolysate; performing ultrafiltration on the earthworm hydrolysate through an ultrafiltration membrane of which the molecular weight cutoff is less than or equal to 30000 Dalton, and freeze-drying to obtain the earthworm extract. By adopting the method, original fishy smell of earthworms is removed in the obtained earthworm extract. The earthworm extract has remarkable antioxidant activity, can be used for effectively promoting the growth and proliferation of human skin fibroblasts and promoting the synthesis of substrates such as collagen to achieve the effect of resisting aging, and can be applied to development of cosmetics and health products having the effects of resisting skin aging.

The present invention relates to a natural plant antioxidant and a preparation method of the natural plant antioxidant. Take fresh tea, green tea, and mimosa at 20-30: 50-60: 10-15 weight percentage as raw materials, add water in weight equal to 2-8 times of total weight of the raw materials, extract two times with a hot reflux low-temperature extractor / thickener, mix the extracts obtained in the two times, filter the mixed liquor, concentrate the filtrate in an external circulating concentrator, and then purify and separate, dry by mist spraying, and crush in a multi-function crush in a clean area, to obtain a fine powder product at 120-200# granularity - natural plant antioxidant. The product can be widely used as food additives, and can not only enhance resistance to oxidation and prolong shelf life of the food but also improve immunity of human body against cancer, cardiovascular diseases, and apoplexy, etc.

The invention discloses a sandworm polypeptide enteric capsule. A sandworm polypeptide capsule is used as an antioxidant material which is applied to eliminating human body free radicals and is absorbed in a weak basic environment in the intestinal tract, so that the absorption rate and the bioavailability are improved. The sandworm polypeptide is extracted by steps of sandworm washing, enzyme hydrolysis, microfiltration, dialysis and the like. Then, the sandworm polypeptide powder is used together with mannitol, magnesium stearate and sodium deoxycholate to prepare the enteric capsule. The sandworm polypeptide enteric capsule provided by the invention has the antioxidant function, can eliminate human body free radicals, and is high in bioavailability and extremely easy to absorb.

The invention relates to a navodon septentrionalis fish-skin antioxidant collagen peptide and use thereof, and the navodon septentrionalis fish-skin antioxidant collagen peptide is characterized in that: the amino acid sequence is Phe-Ile-Gly-Pro-Trp, and the molecular weight is 618.75 Da ((M + H) + 619.63Da) given by ESI (electronic spray ion)-MS (mass spectrum) detection. The preparation method of the navodon septentrionalis fish-skin antioxidant collagen peptide includes navodon septentrionalis fish-skin pretreatment, enzymolysis, ultrafiltration and chromatography and other steps. The prepared antioxidative peptide Phe-Ile-Gly-Pro-Trp has good scavenging effect on DPPH (1,1-diphenyl-2-picryl-hydrazyl) free radical, hydroxyl radical and superoxide anion free radical; at the same time, the Phe-Ile-Gly-Pro-Trp also shows good lipid peroxidation inhibition effect. The Phe-Ile-Gly-Pro-Trp has significant antioxidant activity, and can be used as medicines, health food or food additives.

The invention discloses a preparation method for a corn protein film with a high antioxidation characteristic. The preparation method comprises the following steps: adding water in corn protein powder, carrying out extruding by using a twin screw extruder and carrying out preprocessing; subjecting garlic to peeling and crushing, adding deionized water and carrying out extraction, filtration, concentration and drying so as to obtain an aqueous garlic extract; adding extruded corn protein powder into an aqueous solution of ethanol and carrying out extraction, concentration and drying so as to obtain zein; and adding the aqueous garlic extract and the aqueous solution of ethanol into the zein, carrying out stirring, adding glycerol, continuing stirring and carrying out coating so as to obtain the corn protein film with the high antioxidation characteristic. According to results of experiments in the invention, the corn protein film with the high antioxidation characteristic has high capability in removing DPPH free radicals and a high inhibitory effect on lipid peroxidation.

The invention relates to the technical field of marine chemical engineering, in particular to chitosan quaternary ammonium salt containing organic acid salt as well as a preparation method and application thereof. Chitosan, 3-chloro-2-hydroxypropyltrimethyl ammonium chloride, sodium hydroxide, isopropanol, gallic acid, caffeic acid and ascorbic acid are used as raw materials; firstly, the chitosanis dispersed in isopropanol for swelling expansion; at room temperature stirring is performed for 4h; 3-chloro-2-hydroxypropyltrimethyl ammonium chloride and a sodium hydroxide solution are added; continuous reaction is performed for 12h; then, a proper amount of ethyl alcohol is used for precipitation; the chitosan quaternary ammonium salt is obtained; finally, the chitosan quaternary ammonium salt is dissolved in water; ion exchange with the organic acid salt is performed; a final product is obtained. The chitosan quaternary ammonium salt has the advantages that active groups in derivativesare added; the activity is high; the preparation process is simple; side reaction is little; the cost of the used materials is low. The research proves that the derivatives have good water solubility; the antioxidant activity is high; wide application values are realized in the industry of medicine and functional food health care, particularly cosmetics industry.

The producing method and usages of the selenium maca polysaccharide are shown below. The above mentioned producing method includes three steps. The first step is to put the maca polysaccharide into the acid water solution and dissolve. Then put in the selenide agent and heat for reaction. And then the selenide reaction solution is achieved. The second step is to cool down the selenide reaction solution achieved in the first step. Then separate the solid and liquid. Collect the upper clean liquid and conduct the purification. The third step is to concentrate the upper liquid achieved in the first step. Dry it and then the selenium maca polysaccharide is achieved. The selenium maca polysaccharide produced by this way has both functions of the maca polysaccharide and the organic selenium. it has improved the additional value of the maca polysaccharide so that it can be well absorbed and used by the bodies. The product shows higher biological activity and the application future is quite wide.

The invention provides a lycium ruthenicum anthocyanin crude extract and a controlled-release microcapsule thereof. The anthocyanin crude extract has good antioxidant activity, so that the further purification for an anthocyanin monomeric compound is prevented, and meanwhile, a preparation method is simple and convenient, production materials are easily available, and the production and use costs of the lycium ruthenicum anthocyanin are reduced. The controlled-release microcapsule is an eight-hour controlled-release capsule, the drug release is uniform in speed and complete, the bioavailability of the drug is improved, and the utilization ratio of the drug is guaranteed.

The invention relates to the field of micro-algal oil DHA microencapsulation processing, specifically to microencapsulated DHA micro-algal oil containing antioxidant peptides and a preparation method thereof. Whey protein concentrate and Arabic gum serve as microcapsule wall materials, the antioxidant peptides generated by fermentation of natural whey protein are added into the DHA micro-algal oil, a complex-coacervation method and a spraying drying method are utilized to prepare the microencapsulated DHA micro-algal oil containing the antioxidant peptides, and the shortcoming of low oxidation stability caused by the fact that the DHA micro-algal oil oxidizes easily is overcome. After the prepared DHA micro-algal oil is stored for 25 days, the peroxide value of the DHA micro-algal oil is reduced by 8 meq / kg or above compared with when no DHA micro-algal oil without the antioxidant peptides, and the microencapsulated DHA micro-algal oil is high in oxidation stability and high in sensory value. The prepared DHA micro-algal oil is wide in applied range, can serve as functional factors to be applied to industries such as medicine, food and feed, particularly to infant foods having strict regulations on additives, and the microencapsulated DHA micro-algal oil is favorable for improving memory and enhancing brain development and the like.

The invention discloses hair-strengthening nutrient solution containing an alpine plant extract. The nutrient solution comprises a component A, a component B and a component C. The component A is prepared from, by weight, 60-100 parts of deionized water and 0.01-0.05 part of a chelating agent. The component B is prepared from, by weight, 0.1-0.5 part of a pH regulating agent, 1-5 parts of a cacumen platycladi extract liquid, 0.5-1.5 parts of panthenol, 1-5 parts of a compound plant pilatory, 0.5-1 part of pyridoxine HCl, 0.1-1 part of nicotinamide, 0.01-0.2 part of the alpine plant extract, 0.01-2 parts of a ginger root extract, 0.4-1 part of a plant allergy-alleviating component, 0.01-0.5 part of a asiaticoside compound component, 0.01-2 parts of a moisturizing component and 0.0001-0.001part of a radix polygoni multiflori extract. The component C comprises, by weight, 2-3 parts of an antibacterial component. The hair-strengthening nutrient solution comprises traditional herbal medicine components for preventing hair loss and strengthening hair and is capable of replenishing hair root energy, timely repairing with the nutritional components and reducing scalp grease to make hair thick, black and high in strength.

A preparation method for a nano lipidosome containing a green tea extractive belongs to the field of chemistry. The nano lipidosome comprises the green tea extractive, soya lecithin and cholesterol; the preparation method comprises the following steps: proportionally dissolving soya lecithin and cholesterol in chloroform to obtain a mixed solution, obtaining a transparent film through reduction vaporization, adding a phosphate buffer salt solution containing the green tea extractive, rotating till the film is dissolved, removing the remaining solvent, and performing ultrasonic processing to obtain a nano lipidosome suspension containing the green tea extractive. According to the invention, encapsulation efficiency and average particle diameter are taken as the indexes, the process parameters and the optimal prescription in preparation of the nano lipidosome containing the green tea extractive are determined through single factor experiments and orthogonal experiments. Compared with a collagen membrane added in a common green tea extractive, oxidation resistance stability, mechanical property, water and light resistance, and hydrophobicity of the collagen film added in the nano lipidosome containing the green tea extractive and prepared through the method are all improved, so that relatively good effects of food protection and environmental pollution reduction can be achieved.

The invention relates to a medicine composition of myricetrin or / and myricetin aglycone thereof and levodopa or of levodopa and carbidopa and an application of myricetrin or / and myricetin aglycone thereof as a synergist in preparation of a medicine used for treating Parkinson. In vitro activity determination finds that IC50 of myricetrin and myricetin inhibiting catechol-O-methyltransferase (COMT) can be a micromole-nanomole scale. Rat overall pharmacokinetic study finds that blood concentration of levodopa can be increased after myricetrin and myricetin aglycone thereof and levodopa are used jointly; and myricetrin and myricetin aglycone thereof are high in safety, no obvious toxicity is produced to main visceral organ cells of a human body, and no active intermediate is produced by virtue of metabolism activation and further no damage done to organs such as liver and kidney is initiated, so that the myricetrin and myricetin aglycone thereof have a good application prospect in adjuvant therapy of Parkinson.

The invention provides a skin-whitening toner containing earthworm extracting solution. The skin-whitening toner is characterized by being prepared from the following components by weight: 1.5-2.5 g of rhizoma smilacis glabrae extract, 4-6 g of wax gourd powder, 55-65 g of the earthworm extracting solution, 2-4 g of uronic acid, 1.5-2.5 g of an emulsifier, 1.5-2.5 g of a humectant, 1.5-2.5 g of white grape wine, 2-4 g of rhizoma dioscoreae juice, 4-6 g of radix sophorae flavescentis gel, 0.5-1.5 g of glycerol, 1.5-2.5 g of Vaseline, 2-4 g of lotus root starch, 0.4-0.6 g of protein zinc, 1.5-2.5 g of esterifying enzyme compound bacteria solution, and 30-50 g of purified water. The skin-whitening toner contains polypeptide, amino acid, protein, lumbricin, vitamin and various microelements, has the obvious antioxidant activity, can effectively promote the growth and proliferation of human skin fibroblasts, and promote the synthesis of the matrix such as collagen, and finally, the purposes of whitening the skin and resisting ageing are achieved.

The invention discloses a preparation method of mullet protein source antioxidant peptide. The method comprises the following steps: preparing fresh mullet into minced fish, performing freeze drying on the minced fish, and crushing the minced fish, so as to obtain freeze-dried fish meal; defatting the freeze-dried fish meal through ethyl acetate, performing freeze drying, and curshing, so as to obtain defatted mullet protein power (GM); performing enzymolysis on the defatted mullet protein power (GM), performing centrifugation, and performing freeze drying on obtained centrifugate, so as to obtain crude antioxidant peptide (HGM). The crude antioxidant peptide (HGM) is further separated and purified, so that the mullet protein source antioxidant peptide is obtained. Through the method, mullet protein source antioxidant peptide with high antioxidant activity can be obtained.

The invention discloses an isobenzofuran compound as well as a preparation method and application thereof. The isobenzofuran compound is obtained by separating from a traditional Chinese herbal medicine radix puerariae; the compound is named as 5-methoxy-2,2-dimethyl-2H-furo[3,4-g] chromen-8(6H)-one as an English name, and a molecular formula of the compound is C14H14O4; a structural formula of the compound is shown in the description. The preparation method of the isobenzofuran compound comprises the following steps: using the traditional Chinese herbal medicine radix puerariae as a raw material and carrying out extractum extraction, organic solvent extraction, MCI decolorizing, silica-gel column chromatography and high pressure liquid chromatography separation to obtain a finished product; the isobenzofuran compound is subjected to antioxidant activity and free radical removal activity screening, so the isobenzofuran compound has higher antioxidant and free radical removing activities; the compound is used for producing cigarettes and can be used for effectively removing the free radicals in smoke gas of the cigarettes.

The invention discloses healthy rapeseed oil. The healthy rapeseed oil is prepared from, by weight, 60-80 parts of rapeseed oil, 10-20 parts of grape seed oil, 1-3 parts of amomum cardamomum, 0.5-3 parts of cinnamon, 1-5 parts of candied date extract, 1-5 parts of sophora flower bud extract, 1-5 parts of lonicera japonica extract, 1-5 parts of clove extract and 0.1-0.5 part of vitamin E. According to the healthy rapeseed oil, as the grape seed oil, the amomum cardamomum, the cinnamon and garlic are added into common rapeseed oil, the raw smell of the rapeseed oil can be pressed, the mixture is jointly processed, and then sweet perfumes are further diffused all around; meanwhile, the candied date extract, the sophora flower bud extract, the lonicera japonica extract, the clove extract and the vitamin E are added, the multiple kinds of Chinese herbal medicine and the vitamin E have the high antioxidant activity, the shelf life of the rapeseed oil can be effectively prolonged, and meanwhile the healthy rapeseed oil further has the function of replenishing blood, invigorating the stomach, benefiting the lung, regulating the stomach, cooling blood, stopping bleeding, clearing the liver, discharging fire, dispersing wind heat and removing and detoxifying blood poison.

The invention discloses a method for extracting proanthocyanidin A2 from peanut coat, the method comprises the following steps: (1) using the peanut coat as a raw material, heating with water, extracting and filtering to obtain peanut coat extract; (2) first adsorbing the peanut coat extract by macroporous resin, pre-washing the macroporous resin with an alcohol solution as a pre-washing liquid, and desorbing by using an alcohol solution as a desorbing solution to obtain desorbed crude proanthocyanidin A2; and (3) finely separating the crude proanthocyanidin A2 by medium and low pressure preparative chromatography, after chromatographic separation, concentrating under reduced pressure and drying eluate to obtain the proanthocyanidin A2. The proanthocyanidin A2 obtained by the method has strong antioxidant activity, and the purity reaches 85% or more. The method is of great significance for promoting the deep processing and utilization of the peanut coat, enhancing the added value of peanut products and promoting the sustainable development of the industry.

The invention discloses a cherry enzyme preparation method. The cherry enzyme preparation method includes pretreatment, enzymolysis, matrix preparation, fermentation and concentration and drying of cherries to obtain cherry enzyme. The cherry enzyme contains nutritional ingredients rich in enzyme, vitamin, mineral substance, secondary metabolite and the like and has functions of being anti-aging, antisepsis and anti-inflammation, enhancing body immunity, restoring the damage of organism body injuries and the like.

The invention relates to a method for preparing a liquid mycelium fermentation culture medium formula of selenium-rich cordyceps militaris. The method takes natural colored silkworm cocoon sericin protein and a selenium-rich yeast as a main nitrogen source, takes mixed coarse food grains including rice, Chinese yam, potatoes, wheat, millet and corn as a main carbon source, takes organic selenium in the selenium-rich yeast as a selenium source, takes lentinan and adenosine as motivating factors, and takes other trace factors as auxiliary factors to manufacture the liquid mycelium fermentation culture medium formula of selenium-rich cordyceps militaris. The method effectively recycles abandoned husks and cocoon shells, the cost of the nitrogen source is greatly reduced, the nitrogen source in a culture medium has good bacterial resistance and antioxidant activity, and the fermentation process is not easily contaminated by bacteria. The selenium-rich yeast in the culture medium plays a role of supplementing the nitrogen source and is also a selenium source. Through the addition of the lentinan and the adenosine, the metabolism and growth of selenium-rich cordyceps militaris mycelium can be significantly improved, and the output of active materials such as cordyceps polysaccharide, cordycepic acid, cordycepin and the like is greatly increased.

The invention discloses a cosmetic containing active peptide. The cosmetic consists of the following raw materials in parts by weight: 12-16 parts of glycerol, 0.05-0.08 part of a seaweed extract, 2-4 parts of chitosan, 0.2-0.5 part of triethanolamine, 20-30 parts of an aloe extract, 1-2 parts of hexa-peptide, 1-4 parts of an extract of herba leonuri, 0.8-1.5 parts of an extract of radix curcumae longae, 0.5-1.5 parts of 1,2-hexanediol, 3-4 parts of 3,3-dihydroxyl methyl butane-2-hydroxy propionate, 50-70 parts of water, 5-15 parts of the active peptide, 5-8 parts of hyaluronic acid and 10-20 parts of VC. The cosmetic disclosed by the invention is good in effect and free from side effects, and the cosmetic cannot cause damage to consumers' body health.

The invention discloses vibration massage eye cream containing a ganoderma lucidum fermentation filtrate. The vibration massage eye cream is prepared from the components in parts by weight: a component A: deionized water, a thickener, a humectant, a preservative and sodium hyaluronate, a component B: an emollient, jojoba seed oil, wheat germ oil, an emulsifier, tocopherol acetate, ascorbyl tetraisopalmitate and tocopherol, a component C: a alpine vegetation extract, a centella asiatica extract, the ganoderma lucidum fermentation filtrate, ubiquinone, a pHconditioning agent and the deionized water, and a component D: pullulan, a wild soy protein, essential oil and acetyl tetrapeptide-5. According to the vibration massage eye cream, traditional Chinese medicine herbs and high-end scientificand technological ingredients are combined, the vibration massage eye cream is rich in Chinese ganoderma lucidum fermentation filtrate and other plant active substances and other functional ingredients, dark circles under eyes are reduced, skin elasticity is improved, synthesis of collagen is promoted, vitality of eye cells is improved, and thus skin around the eyes looks more elastic and youthful.

The invention discloses whitening emulsion containing magnolia-biondii extract. The whitening emulsion is prepared from A components, B components and C components. A preparing method of the emulsionincludes the steps that 1) the A components with the formula quantity are weighed, put into a water phase pot, stirred, heated to 80 DEG C to 85 DEG C and completely dissolved ready for use; 2) the Bcomponents with the formula quantity are weighed, put into an oil phase pot, stirred, heated to 65 DEG C to 75 DEG C and completely dissolved ready for use; 3) the water-phase materials obtained in the step 1) are sucked into an emulsifying kettle and stirred, the temperature is reduced to 70 DEG C, the oil-phase materials obtained in the step 2) are sucked into the emulsifying kettle and homogenized for 1 min to 5 min and continue to be stirred, and the temperature is reduced; 4) when the temperature is reduced to 45 DEG C, the C components are added, and the mixture is stirred to be even; 5)when the temperature is reduced to 40 DEG C or below, materials are filtered out. The whitening emulsion can meet the requirements of persons for natural, skin whitening and healthy skin care products, and meanwhile the invention provides the preparing method of the whitening emulsion.

The invention discloses procyanidin A2 and a preparation method and application thereof. Procyanidin A2 has a structural formula as represented by formula (I). According to the invention, a procyanidin compound--procyanidin A2 is prepared and separated from litchi liquid, and yield of procyanidin A2 is as high as 1.26 to 5.26 g / kg (while purity is 80 to 90%); results of activity experiments show that the compound has good antioxidant activity which is substantially better than that of a synthesized anti-oxidant BHT frequently used in the food industry and the compound can be used for preparation of an anti-oxidant or free-radical scavenger; moreover, the compound has good antineoplastic activity, can effectively inhibit propagation of human liver cancer Hep-G2 cells and human cervical carcinoma Hela cells, is used for preparation of antitumor drugs and has wide application prospects. The invention is of critical significance to promotion of deep processing of litchi, increase of added values of litchi products and promotion of sustainable development of the processing industry of litchi.

The invention discloses a compound anti-oxidation functional food additive which comprises the following raw materials: a citrus reticulata extract and a wax myrtle extract, wherein the weight ratio of the citrus reticulata extract to the wax myrtle extract is 1:0.1-10. The method for preparing a compound anti-oxidation functional food containing the additive comprises the following steps: mixing the citrus reticulata extract and the wax myrtle extract, adding auxiliary materials, or adding no auxiliary materials, preparing tablets and granules after mixing evenly, or loading into capsules to prepare capsules so as to prepare a finished product. The compound anti-oxidation functional food additive has obvious anti-oxidation activity and no toxic or side effect, and the method for preparing the compound anti-oxidation functional food of the compound anti-oxidation functional food additive has simple and convenient operation steps and is suitable for industrialized production.

The invention discloses a natural oxidation inhibitor containing plant extracts, and a preparation method and application of the natural oxidation inhibitor, as well as a processing method of minced pork. The natural oxidation inhibitor is prepared by directly and uniformly mixing the following raw materials in parts by weight: 10-15 parts of a sesame-send cake meal extract, 40-75 parts of a grape seed meal extract, and 15-45 parts of a Huangshan maofeng tea extract according to a compounding ratio. The natural oxidation inhibitor is added to the processing course of the minced pork, so that the oxidation of fat and protein in the minced pork can be notably restrained.

The invention discloses a collagen gene functional fragment cloned from a turtle and shown in sequence SEQ ID NO.1 in a sequence table and a recombinant protein obtained by the expression of the clone gene functional fragment and shown in sequence SEQ ID NO.2 in the sequence table, wherein the recombinant protein is a recombinant turtle collagen gene functional peptide fragment. The whole length of the recombinant turtle collagen gene functional peptide fragment includes 252 amino acids, 27 specific Gly-X-Y repeating fields of collagen are contained and the molecular weight is 38kDa. The preparation method comprises the steps of constructing a colibacillus genetic engineering bacterium for expressing the recombinant turtle collagen gene functional peptide fragment; culturing the colibacillus genetic engineering bacterium; inducing and expressing the recombinant turtle collagen gene functional peptide fragment; performing purification renaturation on the recombinant turtle collagen gene functional peptide fragment. The experiment shows that the recombinant turtle collagen gene functional peptide fragment prepared by the method provided by the invention has a high antioxidant activity and also has a value of application as an antioxidant.

The invention relates to a method for preparing bamboo leaf ferment and a bamboo leaf ferment beverage with bamboo leaf submicron powder, and belongs to the engineering field of fermentation techniques. The method comprises the following steps of (1) pretreating raw materials; (2) performing ultrafine crushing; (3) performing pressure treatment; (4) performing enzymolysis; (5) mixing materials; (6) performing microbial fermentation; and (7) performing fermentation post-treatment. The bamboo leaf submicron powder ferment prepared by the method is free from any preservatives and additives, through microbial fermentation, and under the action of beneficial bacterial flora of lactic acid bacteria, microzymes and the like, composite fermentation is performed, so that purely natural bamboo leafsubmicron powder ferment is obtained. According to the method, active components in bamboo leaves can be well reserved, and the bamboo leaf ferment is rich in nutrients, natural, environment-friendly,and suitable for industrialized production.