263 results about "Functional peptide" patented technology

The present invention provides a substantially purified growth differentiation factor (GDF) receptor, including a GDF-8 (myostatin) receptor, as well as functional peptide portions thereof. In addition, the invention provides a virtual representation of a GDF receptor or a functional peptide portion thereof. The present invention also provides a method of modulating an effect of myostatin on a cell by contacting the cell with an agent that affects myostatin signal transduction in the cell. In addition, the invention provides a method of ameliorating the severity of a pathologic condition, which is characterized, at least in part, by an abnormal amount, development or metabolic activity of muscle or adipose tissue in a subject, by modulating myostatin signal transduction in a muscle cell or an adipose tissue cell in the subject. The invention also provides a method of modulating the growth of muscle tissue or adipose tissue in a eukaryotic organism by administering an agent that affects myostatin signal transduction to the organism.

It is intended to provide a peptide or a phage recognizing nanographite structures and thus enabling efficient recognition, binding, separation and alignment of nanographite structures such as carbon nanohoms or carbon nanotubes, an artificial protein or a chimeric molecule comprising the above-described peptide bonded to a functional peptide, a protein, a labeling, etc., and a complex of the above-described peptide molecule, artificial protein or chimeric molecule with a nanographite structure. By panning peptide-presenting phages bonded to nanographite structures, a nanographite structure-binding peptide capable of specifically recognizing nanographite structures such as carbon nanohoms or carbon nanotubes is obtained.

By inhibiting the function of a transcription factor that promotes transcription of a gene associated with the amounts of lignin and cellulose, a plant in which the amounts of lignin and cellulose are reduced without reducing the amount of glucan is produced. In this plant, glucan in the obtained cell wall components is in the state of highly easily undergoing saccharification. In this plant, moreover, natural dehiscence of pods is suppressed. A method of inhibiting the transcription factor includes a method in which a chimeric gene between a transcription factor gene and a polynucleotide that encodes a functional peptide capable of converting the transcription factor into a transcription repressor is introduced into a plant cell so that a chimeric protein in which the transcription factor is fused with the functional peptide is produced in a plant cell, and a method of inhibiting the expression of the transcription factor, such as knockout method or RNAi method. Thus, a plant in which the amounts of lignin and cellulose are reduced without reducing the amount of glucan is provided.

The invention provides functional soy peptide fermented milk and a preparation method thereof and relates to a manufacturing method of fermented dairy products in the field of food processing. The method is carried out according to the following steps: (1) the preparation of fermented base stock; (2) the preparation of mother cultures; (3) synchronous enzymolysis and fermentation; (4) blending and homogenizing; (5) ultrahigh pressure sterilization and filling; by adopting the steps, a finished product is obtained. The method adopts protease and lactic acid bacteria synchronous-enzymolysis fermentation technology, and ultrasonic waves are adopted to process slurry-residue mixture which is ground, so as to increase the dissolving-out rate of the protein and prepare acidity soy-bean milk by ultrahigh pressure and non-heat processing. The product taste is coordinated without enzymolysis odour; the enzymolysis fermentation time can shorten to 2-4h, and the production cost is low. The ultrahigh pressure processing can remarkably improve the product taste; the method can keep and produce new functional peptide, the lactic acid bacteria is inhibited to ferment continuously, and a certain micro-organism viable counts are kept; the shelf life of the acidity soy-bean milk is prolonged, so as to prevent the acid milk from being whey-separated owning to continuous increasing of acidity; inaddition, the product can be stored and marketed at normal temperature.

The present invention includes peptidomimetic compound compositions and methods of making and using peptidomimetic compounds to modulate the activity of a peptide receptor for the treatment of one or more of hyperglycemia, insulin resistance, hyperinsulinemia, obesity, hyperlipidemia, hyperlipoproteinemia or other symptoms that relate to the function of the targeted receptor. The peptidomimetic includes an oligo-benzamide compound having at least three optionally substituted benzamides.

The invention discloses a method for producing fermentation bean pulp rich in feeding use functional peptides. The present invention relates to a manufacturing technique taking the dehulled bean pulp as raw materials, using the microbial fermentation technology to remove various nutrilit-resistance in the bean pulp and simultaneously obtaining a great deal of polypeptide fermentation bean pulps, specifically to a microbial fermentation process using multi-strain mixed solid fermentation for preparing bean pulp rich in feeding use functional peptides. The manufacturing technique including mixing uniformly a fermentation nutrient fluid composed of three strains of expansively trained Bacillus subtilis, one strain of Saccharomyces crevisiae and one strain of Lactobacillus according to the proportion 1.8:1:1; inoculating 5% of the inoculated amount into the cottonseed pulp the water content of 50% ( sieving with a 40 mesh sieve), simultaneously adding 5% of molasses, and mixing uniformly (with initial pH value of 7.0); aerobic fermentation at temperature of 40 DEG C for 48 hours, and then performing dry sampling at 60 DEG C, and finally performing nutrition component analysis to the bean pulp fore and after fermentation. The completed product of the fermentation bean pulp can be stored in a cool dry place for 6-9 months after weighing and packaging, which can be the feeds of pig, chicken, tilapia and other animals.

Transcription of a gene associated with formation of floral organs is suppressed to produce a sterile plant. A plant cell is transfected with a chimeric gene that includes (i) a coding gene of a transcription factor that promotes expression of a gene associated with formation of floral organs, and (ii) a polynucleotide that encodes a functional peptide that converts an arbitrary transcription factor into a transcription repressor, and a chimeric protein in which the transcription factor is fused with the functional peptide is expressed in the plant cell. The expression of the gene associated with formation of floral organs is dominantly suppressed by the chimeric protein, and as a result a male sterile plant is produced that cannot properly form pollen. The chimeric protein also suppresses expression of a gene associated with dehiscence of anther, and as a result a plant is produced in which dehiscence of anther is suppressed. Further, the chimeric protein suppresses expression of target genes of a transcription factor associated with formation of stamen and pistil, and as a result a double flowered plant is produced.

The invention discloses a compound type biological active peptide product for livestock and poultry, which is formed by mixing the following components in percent by mass: 1%-7% of functional active peptide, 10%-40% of probiotics, 5%-20% of prebiotics, and the balance of auxiliary materials, wherein the functional peptide is one or a combination of several of collagen peptide, glutathione, muramyl dipeptide, soybean protein peptide and casein phosphopeptide. The probiotics refers to one or a combination of several of lactic acid bacteria, saccharomycetes, photosynthetic bacteria, actinomycetes, bacillus and streptococcus faecalis. The prebiotics is one or a combination of several of isomalto-oligosaccharide, fructo-oligosaccharide, galactooligosaccharide and xylo-oligosaccharide. The auxiliary materials refer to one or a combination of several of silkworm chrysalis, fish meal, montmorillonite, diatomite, zeolite powder, corn flour, bran and bean pulp. Additive amount of the product accounts for 0.1%-0.2% of the amount of feed, and the feed can be directly fed after the product is added and uniformly mixed. The compound type biological active peptide product disclosed by the invention belongs to the micro-ecological technical product and is green and environment-friendly.

According to the present invention, a gene having a novel function that can cause an increase or decrease in seed protein content is searched for. A chimeric protein obtained by fusing a transcription factor consisting of a protein comprising an amino acid sequence shown in any of the even-numbered SEQ ID NOS: 1 to 76 and a functional peptide capable of converting an arbitrary transcription factor into a transcriptional repressor or a transcription factor consisting of a protein comprising an amino acid sequence shown in any of the even-numbered SEQ ID NOS: 77 to 84 is expressed in a plant.

An object of the present invention is to search for a gene having a novel function that can cause an increase or decrease in material productivity, and particularly, fat and oil content. In the present invention, a chimeric protein obtained by fusing a transcription factor consisting of a protein comprising an amino acid sequence shown in any of the even-numbered SEQ ID NOS: 1 to 158 and a functional peptide capable of converting an arbitrary transcription factor into a transcriptional repressor is expressed in a plant.

The present invention relates to provide a protein group binding to keratin which is the major component of body hair, or genes encoding the same, particularly to keratin-associated proteins (KAP) which bind specifically and strongly to hair keratin or genes encoding the same. The base sequence of eurochromtic region of approximately 33.5 Mb of human chromosome 21 was determined, a dot-matrix analysis of the base sequence of the long arm region of chromosome 21 (21q22.3) was carried out, homology search was made to low frequency repetitive sequences and 16 KAP genes being expressed only in hair root cells were found. Moreover, the high frequency repetitive sequences present in the sequence spanning for approximately 1 Mb between CLDN8 gene and TIAM1 gene in the long arm region of chromosome 21 (21q22.11) were masked, the presence or absence of short low frequency repetitive sequence was searched, and 22 KAP genes were found. Moreover, a group of functional peptide was designed from the above mentioned KAPs.

This invention provides a functional peptide fiber which comprises a plurality of peptide structure units each containing at least one peptide chain, wherein peptide chains contained in each adjacent peptide structure units do not form peptide bond but are structured into a fibrous form by taking a beta-sheet structure, and wherein at least one of the plurality of peptide structure units contains a peptide chain having a functional material connected thereto. Also disclosed are a method for producing the functional peptide fiber and a method for recovering peptide chains from the functional peptide fiber.

A gene having novel functions is searched for, by which plant weight (that is, biomass level) can be increased and by which substance productivity can be increased or decreased. A chimeric protein is expressed in which a transcriptional factor comprising the amino acid sequence shown in SEQ ID NO: 2, 4, or 6 is fused to a functional peptide that converts an arbitrary transcriptional factor into a transcriptional repression factor.

The present invention relates to a method for synthesizing peptide conjugates comprising a functional peptide, cyclic by means of a lactam bridge; a sulfur-linker bound to said cyclic peptide, wherein said sulfur-linker comprises sulfur or a sulfur-reactive site. The invention further relates to a method for synthesizing peptide-effector conjugates. The invention also relates to peptide conjugates. Peptide conjugates according to the present invention have improved half-life and increased availability at the active site and they are useful in cell targeting and tumor diagnosis and therapy.

The invention belongs to the technical field of medicine, and relates to an acetylcholine-receptor-mediated trimethyl chitosan-graft-polyethylene glycol / nucleic acid brain-targeting micellar and a preparation method thereof. According to the invention, trimethyl chitosan-graft-polyethylene glycol-brain-targeting functional peptide is obtained through the steps that: chitosan is sequentially subjected to quaternary ammonium modification such that trimethyl chitosan is obtained; the amino group of trimethyl chitosan is subjected to a reaction with an active group of two-end-activated polyethylene glycol, such that trimethyl chitosan-graft-polyethylene glycol is obtained; and another active group of polyethylene glycol is subjected to a reaction with brain-targeting functional peptide RVG. According to the invention, ion composite micellar is adopted as a carrier, and brain-targeting functional peptide RVG is adopted as a targeting molecule, such that nucleic acid medicine can actively target and position through blood-brain barrier and brain. The ion composite micellar provided by the invention is formed through static composition of positively charged trimethyl chitosan-graft-polyethylene glycol-brain-targeting functional peptide and negatively charged nucleic acid. With the invention, nucleic acid medicine defects such as easy in-vivo degradation, poor stability, and low transfection efficiency are solved.

The invention discloses a functional peptide with the cosmetic efficacy and a preparation method and an application thereof. The functional peptide is a composite product of an elastin peptide and a collagen peptide, and the preparation method of the functional peptide with the cosmetic efficacy includes the following steps: soaking elastin raw materials with a NaCl solution to remove oil, and soaking collagen raw materials with a NaOH solution to remove oil; then conducting centrifugation or mincing to obtain an elastin crude extract and a collagen crude extract, and then mixing the elastin crude extract with the collagen crude extract, conducting enzymatic hydrolyzing with protease, and then spray drying the obtained enzymatic hydrolysate to obtain the functional peptide with the cosmetic efficacy. According to the preparation method, the elastin and collagen are simultaneously treated by a biological enzymolysis technology, while the elastin peptide and the collagen peptide with the cosmetic efficacy are released, the elastin peptide and the collagen peptide are better mixed during the enzymatic hydrolysis process. The composite product of the elastin peptide and the collagen peptide, prepared by the method, has the obvious efficacy of moisturizing and resisting wrinkle, and can significantly improve the moisture content and the collagen content in the skin.

The invention provides a method for producing fermented soybean meal by two-step inoculation segmented solid-state fermentation. The method comprises the following steps of: inoculating the mixed bacterial solution obtained by mixing bacillus subtilis solution, candida mycoderma bacteria solution and lactobacillus solution in peeled soybean meal and performing mixed fermentation; and inoculating the lactobacillus solution in the fermented product, mixing, and performing anaerobic fermentation to obtain the fermented soybean meal. The method is capable of meeting the different needs of aerobic bacteria and anaerobic bacteria, can be used for preparing high-quality fermented soybean meal, and is free of chemical additives; due to the various digestive enzymes, organic acids, medium and small molecular proteins (functional peptides), amino acids, vitamins, probiotics and the like generated during the fermentation process, the nutritive value of the soybean meal is greatly increased; the prepared fermented soybean meal contains not less than 16% of soybean peptide, not more than 25mg / g of glycinin, not more than 20mg / g of beta-conglycinin, not less than 3.5% of lactic acid, and not less than 2*10<9> beneficial viable bacteria; and microbial source nutrients are beneficial to the maintenance of intestinal structure and can promote immunologic function and improve animal production performance.

The invention discloses an epididymis protease inhibitor which is a functional peptide segment of Eppin. The inhibitor is any one selected from amino acid sequences shown from SEQ ID No.1 to SEQ ID No.7 and has advantages of single component, simple structure and strong pertinence of immunoreaction inspired; polypeptide with at least 80 percent of sequences the same as the functional peptide segment of Eppin can be used for constructing immunogen which can improve the immunogenicity of the functional peptide segment of Eppin, stimulate an organism to generate a specific antibody, reduce Eppin level in blood plasma and induce high-efficient, safe and reversible antifertility effect. The immunogen of the epididymis protease inhibitor can form a medicament composite with medically acceptable carriers, and the composite can be used for preparing male contraception vaccines, therefore, the epididymis protease inhibitor has broad application prospect.

The invention discloses a construction method of an artificial blood vessel, and the method particularly includes the step that both the interior and the exterior of a polyester knitting tube are evenly coated with regenerated silk fibroin introducing two types of functional peptides. According to one of the functional peptides, the chain side base contains a great number of hydrophilic carboxy groups, and the acidic amino acid and molecules of the peptide are of electronegativity; the other peptide contains eight RGD and promotes cell adhesion. The two types of peptides come from expressions of living bodies, the sequences are analogues from natural protein, no toxicity or irritation exists, the molecular weight is singular, the peptides and the silk fibroin can be arranged on a polyester pipe in a covalent binding and coated mode, the hydrophilic performance and electronegativity are provided for the artificial blood vessel continuously and stably, endothelialization is promoted, harm to cells is lowered, and thrombus blockage caused by proteinosis and blood cell aggregation is prevented. The constructed artificial blood vessel has good biocompatibility and has an electronegative membrane layer similar to a natural blood vessel and a microenvironment for promoting adhesion growth of endothelial cells, and accordingly it is beneficial to protect blood cells and prevent thrombus from forming.

The invention discloses a Bacillus velezensis LfF-1 strain and application thereof in protease production. The Bacillus velezensis LfF-1 strain is separated for a first time, the strain is preserved in the Guangdong Microbiological Culture Collection Center in August 16, 2019, and the preservation number is GDMCC NO: 60741. The strain has a remarkable capability for producing protease through fermentation, and types of cultures produced through protease fermentation can be enriched; a protease preparation can be produced by using the strain through a fermentation tank replenishing on-batch fermentation process, the activity of the protease is as high as 17.84 ten thousand U / g, the enzyme activity recovery rate is as high as 96.8%, types of conventional commercial protease can be greatly enriched, and the production efficiency of the protease preparation can be improved; and in addition, the method for producing the protease from the strain through fermentation is simple and feasible, and has wide application prospects in producing protein foods, functional peptide products or feed additives.

The invention discloses a use of a derived polypeptide series of pigment epithelium derived factors in ischemic myocardium protection. The polypeptide is derived from the pigment epithelium derived factors, and every amino acid sequence in the polypeptide includes at least eighteen continuous amino acid residues. The PEDF derived functional peptide has good function advantages, and has remarkablecardiac muscle tissue capillary tube stabilization, vascular leakage prevention, myocardial cell protection and cardiac muscle tissue inflammation prevention effects.

The present invention discloses a method for preparing functional peptide protein powder using blood cells of livestock and poultry as raw materials. The method is as follows: continuously centrifuging fresh blood of livestock and poultry to separate blood plasma and blood cells; processing the blood cells by physical and chemical methods to break cell membranes and denature intracellular protein; after the membrane breaking and denaturing process, adding acid (or base) and a protease preparation into the blood cell solution to enzymatically hydrolyze the blood cell protein into small peptides and amino acids containing functional ingredients; then centrifuging the products of enzymolysis and adding acid and a decolorizer to performing decoloration; and finally performing spray drying on the decolorized blood cell solution. The present invention also provides functional peptide protein powder prepared by the method. The functional peptide protein powder has a high protein content, a high essential amino acid content, a high bioactive peptide content, a high digestion and absorption rate, and a strong antioxidant capacity, can improve the disease resistance of livestock and poultry, protein deposition and production performance, can be widely used for livestock, poultry and aquatic animals, and can also be used as a substitute for fish meal and soybean meal.

The present invention discloses a preparation method capable of utilizing beer yeast produced in the beer production to make yeast peptide. Said method includes the following step: collecting beer yeast, extruding, mixing paste, zymolysis and breaking wall, secondary mixing paste, zymolyzing protein, concentrating, drying, pulverizing so as to obtain the invented yeast peptide. Said yeast peptide can be used as raw material for making multifunctional health-care product.

The invention discloses an enzymolysis-fermentation coupled aquatic protein active peptide preparation method. The method mainly includes the following steps that fresh aquatic protein is minced; the minced aquatic protein is added to an enzymolysis tank, nutrients and water are added, and the mixture is mixed to be uniform to serve as a fermentation culture medium; the enzymolysis tank is inoculated with high-temperature-resistant facultative anaerobe Bacillus methylotrophicus HL-4 capable of generating protease, and meanwhile enzymolysis and fermentation are conducted; fermentation liquor is subjected to filtration, ultra-filtration and nano-filtration in sequence, then concentrated liquor is obtained, and the finished active peptide is obtained after freeze drying of the concentrated liquor. According to the technology, the enzymolysis process and the fermentation process are completed in the enzymolysis tank at the same time, and equipment and operation are simplified; acting conditions are mild, the biological activity of functional components in the prepared functional peptide and the aquatic protein is better preserved, and good application prospects are achieved.

The invention relates to a tumor enzyme activated quantum dot probe, which is modified and contains a functional peptide; wherein one end of the functional peptide is connected to a quenching agent, and the tumor enzyme can specifically recognize and cut off the functional peptide to light the quantum dots. The tumor enzyme activated quantum dot probe is in a fluorescence quenched state in the normal tissue and gives off fluorescence in the tumor part; namely the quantum dot has dual functions: invisibility in the normal tissue and tumor enzyme sensitivity in the tumor part, and thus the probe can diagnose tumor. The invention further provides a preparation method of the quantum dot probe, and an application of the quantum dot probe in preparation of reagents for tumor diagnosis. The provided quantum dot probe can diagnose tumor and is advantageously used as a precursor probe for tumor diagnosis.

The invention discloses a nutritional noodle rich in functional peptides, iron and zinc. The nutritional noodle comprises, by weight, 70-80 parts of wheat flour, 2-10 parts of wheat malt flour, 0.001-0.005 parts of lotus root polysaccharide, 0.006-0.01 parts of rhodioloside, 1.8-2.2 parts of plant oil, 0.01-0.015 parts of bee pollen, 0.1-0.5 parts of a modifier, 0.005-0.008 parts of zinc albuminate and 0.015-0.02 parts of salt. The noodle made in the invention has the characteristics of fineness, tough mouthfeel, boiling resistance and no turbid soup, can obtain a sense organ score of 92, and can reach industrial product high-quality standards.

The present application belongs to the field of functional peptides and more particularly to the field of controlled protein aggregation. The invention discloses molecules of a peptide structure as defined in the claims and methods of using such molecules for therapeutic applications and for diagnostic uses, as well as in other applications such as in the agbio field and in industrial biotechnology. The molecules can be used for curing and / or stabilizing infections such as bacterial,fungal and viral diseases, but are also useful in non-infectious human and veterinary diseases. The molecules can also be used for the detection of protein biomarkers and for the prognosis and diagnosis of a variety of diseases.

The invention aims at providing fishbone fermented milk and a preparation method thereof. Fishbones are subjected to enzymolysis through protease, then hydrolysate and milk are mixed, and lactic acid bacteria are introduced for fermentation so that the fishbone fermented milk can be prepared. The fishbone fermented milk is pure white or light yellow in luster, has the special fresh aroma of aquatic products and milk and is free of odors making people unpleasant; the saline taste, the sweet taste and the delicate flavor are palatable, and the requirements of the popular taste are met; the fishbone fermented milk is uniform and free of suspended solids and flocculent precipitate and contains functional peptide, vitamins, mineral substances, trace elements and other rich nutrient substances. According to another large characteristic of the fishbone fermented milk, the fishbone fermented milk has the calcium supplement function. Close relationship exists among absorption and utilization of calcium, the form of intake calcium, a proper calcium and phosphate ratio and existence of amino acid. Fishbones contain rich calcium and phosphate and other substances, fishbone calcium is converted into precipitate calcium, amio acid calcium, calcium lactate and the types of various kinds of organic calcium, the concentration of soluble calcium and the acidity of bone liquid are increased, calcium is prevented from being precipitated in the intestinal tract, and absorption of a human body to calcium is promoted.

The invention belongs to the technical field of health-care food, and particularly relates to an oviductus ranae functional peptide nutritive jelly and an oviductus ranae enzymolysis byproduct protein nutritive jelly. The jellies adopt oviductus ranae functional peptide and unhydrolyzed oviductus ranae as protein enhancers and the mixture of carrageenan, gelatin and xanthan as gelatinizer, white granulated sugar, citric acid, potassium sorbate and essence are applied to regulate the flavor, the products are clear in color and transparent, the taste is delicate, the elasticity is moderate, and the jellies are chewy; the hardness of the textures is moderate, and air bubbles do not exist; the taste and the smell are moderately sour and sweet, and the jellies do not have the fishy smell of oviductus ranae. The preparation process for the jellies mainly includes two parts, i.e. the preparation of the oviductus ranae functional peptide and the preparation of the protein nutritive jellies. The inspected quality indexes of the protein nutritive jellies disclosed by the invention fully meet product standards, and since the protein nutritive jellies respectively contain the oviductus ranae functional peptide and the collagen, the protein nutritive jellies have the effects of enhancing the immunity of the human body, reducing blood fat and blood sugar, maintaining beauty and the like, and is significant in guaranteeing the physical health of people.

The invention discloses a method for preparing a functional flavor-developing base material from rice protein. According to the method, the rice protein is taken as a raw material, enzymolysis is carried out by using endo alkaline proteases, acid proteases and exo protease segments, and a short-peptide mixture which is rich in flavor amino acids (glutamates and aspartates) and flavor-developing polypeptides is finally obtained and can be widely applied to food and beverage industries as a functional flavoring agent. According to the method, the preparation conditions are mild, harmful substances, such as trichloropropanol and the like, cannot be produced, and the safety is high, so that the method is an ideal alternative method of a vegetable protein acidic hydrolysis method. The flavor-developing base material prepared by the method disclosed by the invention contains a lot of flavor peptides, simultaneously contains abundant of functional peptides, can be quickly absorbed by a humanbody, is beneficial to fatigue recover, and has the functional characteristics of excellent oxidation resistance, improvement on the immunity of the human body and the like.