Enzymolysis-fermentation coupled aquatic protein active peptide preparation method

A protein activity and aquatic technology, applied in the direction of peptide preparation methods, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of weakening biological activity, increasing burden, increasing cost, etc., to reduce Maillard reaction , reduce costs and increase energy consumption

Inactive Publication Date: 2016-06-01
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above high-temperature treatments will weaken the biological activity of the prepared functional peptides or the functional components contained in the aquatic protein itself to a certain extent; in addition, the high-temperature treatment will significantly enhance the Maillard reaction, making the color of the enzymatic hydrolyzate darker, which is suitable for the finished product processing The process increases the burden...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Insert the protease-producing thermostable facultative anaerobic bacteria Bacillus methylotrophicus HL-4 preserved on the slant into the cooled LB liquid culture medium after sterilization, at 55°C, 150r / min and cultured for 12 hours, and prepared into Bacillus methylotrophicus HL-4 seed solution for later use. Mince fresh Antarctic krill for later use. Add 15 kg of minced Antarctic krill and 100 kg of water into the enzymatic hydrolysis tank, and add 100 grams of glucose, 420 grams of food-grade calcium chloride, 350 grams of food-grade ferrous chloride and 550 grams of Food grade dipotassium hydrogen phosphate to obtain fermentation medium; add 8 kg of Bacillusmethylotrophicus HL-4 seed liquid to the fermentation medium in the enzymolysis tank; The time is 46h; after the fermentation, the fermentation liquid is filtered to remove the precipitate to obtain the supernatant; the supernatant is passed through the ultrafiltration of the ultrafiltration membran...

Embodiment 2

[0026] Example 2: Insert the thermostable facultative anaerobic bacteria Bacillus methylotrophicus (Bacillus methylotrophicus) HL-4 preserved on the slant into the cooled LB liquid medium after sterilization, at 55°C, 150r / min and cultured for 12 hours, and prepared into Bacillus methylotrophicus HL-4 seed solution for later use. Mince the head of the fresh vannamei shrimp and set aside. Add 20kg of minced shrimp heads and 100kg of water into the enzymolysis tank, and add 150g of glucose, 430g of food-grade calcium chloride, 380g of food-grade ferrous chloride and 600g of food into the enzymolysis tank Grade dipotassium hydrogen phosphate to obtain fermentation medium; add 8 kg of Bacillusmethylotrophicus HL-4 seed liquid to the fermentation medium in the enzymolysis tank; 42h; after the fermentation, the fermentation broth was filtered to remove the precipitate to obtain the supernatant; the supernatant was passed through the ultrafiltration of the ultrafiltration membrane ...

Embodiment 3

[0027]Example 3: Insert the thermostable facultative anaerobic bacteria Bacillus methylotrophicus (Bacillus methylotrophicus) HL-4 preserved on the slant into the cooled LB liquid culture medium after sterilization, at 55°C, 150r / min and cultured for 12 hours, and prepared into Bacillus methylotrophicus HL-4 seed solution for later use. Fresh oysters are shucked and minced for later use; 22 kg of minced oysters and 100 kg of water are added to the enzymatic hydrolysis tank, and 180 grams of glucose, 480 grams of food-grade calcium chloride, 380 grams of calcium chloride are added to the enzymatic hydrolysis tank. gram of food-grade ferrous chloride and 650 grams of food-grade dipotassium hydrogen phosphate to obtain a fermentation medium; add 8 kilograms of Bacillusmethylotrophicus HL-4 seed liquid to the fermentation medium in the enzymolysis tank; the temperature in the enzymolysis tank is controlled at 53°C , the rotation speed of the enzymolysis tank is 90rpm, and the fer...

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Abstract

The invention discloses an enzymolysis-fermentation coupled aquatic protein active peptide preparation method. The method mainly includes the following steps that fresh aquatic protein is minced; the minced aquatic protein is added to an enzymolysis tank, nutrients and water are added, and the mixture is mixed to be uniform to serve as a fermentation culture medium; the enzymolysis tank is inoculated with high-temperature-resistant facultative anaerobe Bacillus methylotrophicus HL-4 capable of generating protease, and meanwhile enzymolysis and fermentation are conducted; fermentation liquor is subjected to filtration, ultra-filtration and nano-filtration in sequence, then concentrated liquor is obtained, and the finished active peptide is obtained after freeze drying of the concentrated liquor. According to the technology, the enzymolysis process and the fermentation process are completed in the enzymolysis tank at the same time, and equipment and operation are simplified; acting conditions are mild, the biological activity of functional components in the prepared functional peptide and the aquatic protein is better preserved, and good application prospects are achieved.

Description

technical field [0001] The invention relates to an enzymolysis-fermentation coupled method for preparing active peptides of aquatic proteins, which belongs to the field of processing and utilization of aquatic products. Background technique [0002] The small peptides produced by protein enzymatic hydrolysis are not only easy to digest and absorb, but also have anti-hypertensive, anti-cholesterol, anti-thrombosis, improve lipid metabolism, enhance human physical fitness, help restore fatigue, promote calcium, phosphorus and other trace elements. Absorption, promote brain development, improve memory, enhance the vitality of macrophages and B cells, enhance immune function, protect epidermal cells, prevent melanin precipitation, eliminate free radicals in the body, etc. [0003] A lot of research has been done in China on the preparation of aquatic protein active peptides. At present, the preparation methods of aquatic protein active peptides mainly include autolytic enzymati...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34C12R1/225
CPCC07K1/34C12P21/06
Inventor 洪鹏志刘唤明周春霞杨萍
Owner GUANGDONG OCEAN UNIVERSITY
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