96 results about "Facultative anaerobic bacteria" patented technology

Animal waste such as fecal material from swine, chicken, turkey, and cattle is converted into useful forms such as fertilizer, other types of soil builders, and even nutrient feed additives. Devices, systems, and methods are provided that allow economical conversion and in many instances, alleviate the production and release of undesirable gases such as sulfide and ammonia. In one embodiment, undesirable anaerobic and facultative anaerobic bacteria are killed at a greater rate than desirable soil compatible aerobic bacteria. The use of low temperature killing diminishes off gassing commonly associated with other techniques, and the use of gas trapping additives such as zeolites provides enhanced soil building qualities such as slow release of nitrogen and slow release of moisture to soil. The methods allow convenient adjustment of composition, allowing conversion of waste into tailored designer fertilizer suited for particular soils. Other embodiments provide other soil building qualities as well as nutrient qualities for feedstock used in animal husbandry such as aquaculture.

Animal waste such as fecal material from swine, chicken, turkey, and cattle is converted into useful forms such as fertilizer, other types of soil builders, and even nutrient feed additives. Devices, systems, and methods are provided that allow economical conversion and in many instances, alleviate the production and release of undesirable gases such as sulfide and ammonia. In one embodiment, undesirable anaerobic and facultative anaerobic bacteria are killed at a greater rate than desirable soil compatible aerobic bacteria. The use of low temperature killing diminishes off gassing commonly associated with other techniques, and the use of gas trapping additives such as zeolites provides enhanced soil building qualities such as slow release of nitrogen and slow release of moisture to soil. The methods allow convenient adjustment of composition, allowing conversion of waste into tailored designer fertilizer suited for particular soils. Other embodiments provide other soil building qualities as well as nutrient qualities for feedstock used in animal husbandry such as aquaculture.

A device and method for growing aerobic and facultatively anaerobic bacteria such as Pseudomonas Fluorescens, Bacillus subtilis, Bacillus licheniformis, Starkeya novella and various autotrophic sulfur metabolizing bacteria, along with methods for releasing these bacteria into suspended growth or fixed film wastewater treatment zones such as soil or media, for the purposes of bioremediation and the removal of nitrogen, sulfur, and carbon wastes.

The invention is about a kind of magnetotactic bacterium which is separated from the mud sample in the iron-affluent watershed. The bacterium is preserved in the Chinese typical culture collection center. The preserving code is CCTCC NO:M205105 while the classified name is Magnetotactic bacterium MB-TZ. The bacterium is facultative anoxybiontic bacterium and the state of the thallus is circular staff. A thallus can produce 3-20 circular magnetic globules which are mainly comprised by iron, calcium, fosforus, oxygen and magnesium. The bacterium has many advantages such as short producing cycle, easy to cultivate and easy to be produced in factories. During the extracting and purifying of the magnetic globules, the invention adopts the treating method of repeating freeze thawing combining with supersonics, so it can dissolve the thallus more thoroughly and increase the yield of magnetic globules.

The invention belongs to the field of feed packaging, in particular to a bagged fermented feed package for livestock and poultry without antibiotics and high live bacteria. Bagged fermented feed package for livestock and poultry without antibiotics and high live bacteria. The fermented feed package is composed of a packaging bag and a feed base material packed in the packaging bag to inoculate the bacteria. A mixed bacterial solution with a bacterial count greater than 108cfu / g, the mixed bacterial solution is composed of aerobic bacteria and facultative anaerobic bacteria; the packaging bag is equipped with a one-way air outlet valve device. The fermented feed prepared by using the fermented feed package has comprehensive nutrition of the mixture, is fermented, has biological activity, does not remain, and does not produce drug resistance.

The invention discloses an oxygen-rich suspending chain type microporous aerating device. The device comprises an air compressor, a main oxygen preparing machine, a gas inlet pipe, a high tower aerating pond, a three-phase separator and a water absorbing pump; the air compressor is connected with the main oxygen preparing machine; the oxygen preparing machine is used to prepare oxygen by the PSA (Pressure Swing Adsorption) method; one end of the gas inlet pipe is connected with the main oxygen preparing machine and while the other end of the gas inlet pipe extends through the aerating pond; pure oxygen prepared by the main oxygen preparing machine enters the gas inlet pipe; a stop valve and a filter are arranged on the gas inlet pipe; a condensate water draining valve is arranged at the tail end of the gas inlet pipe; at least two gas inlet hoses which are connected with an aerating pipe are connected to the gas inlet pipe; two ends of the aerating pipes are sealed; the aerating pipe is also equipped with falling parts which are symmetrically arranged at two sides of the aerating pipe; the falling parts are suspended on the gas inlet pipe through a suspending chain. According to the oxygen-rich suspending chain type microporous aerating device, blocking is greatly avoided, the aerating effect is great, the oxygenation capacity is high, the generation of some anaerobic bacteria (facultative anaerobic bacteria) can be effectively inhibited, and the strength of ozone is also reduced.

The invention discloses an enclosed rapid culture method of microalgae. The method includes the steps of: (1) cultivating a facultative anaerobic bacteria seed liquid; (2) cultivating an autotrophic microalgae seed liquid; and (3) inoculating the facultative anaerobic bacteria seed liquid and the autotrophic microalgae seed liquid into a closed photobioreactor with a built-in membrane separation device to carry out semi-mixing culture, fermenting the anaerobic bacteria in the membrane separation device, and subjecting the autotrophic microalgae to photosynthesis growth in the photobioreactor outside the membrane separation device, wherein the medium composition and culture conditions are the same in the two reactors, and supplementing organic carbon source required by the growth and metabolism of the facultative anaerobic bacteria in the culture process. The photobioreactor also comprises a guide shell, the membrane separation device is arranged in the guide shell, and a reflective membrane is pasted on the outer surface of the guide shell. The method has the advantages of increasing the yield of autotrophic microalgae, the utilization rate of an inorganic carbon source (CO2), and oil content of the microorganisms, and simplifying the culture device.

The invention discloses a method for preparing composite bacterium, comprising the following steps: inoculating microorganism of zymogenous bacteria, hydrogen-producing acetogenic bacteria and methanogen respectively belonging to facultative anaerobic bacteria and strict anaerobic bacteria which are mixed according to a certain proportion into a culture solution containing cellulose, sealing and keeping standing to culture at a temperature of between 30 to 50 DEG C and pH value of 3 to 10, wherein the culture solution containing cellulose contains CaCO3, 0.3 to 0.7 percent of NaCl, 0.5 to 1 percent of cellulose, 0.1 to 0.4 percent of urea, 0.1 to 0.3 percent of peptone and 0.05 to 0.15 percent of yeast powder, and water is used as a solvent. The composite bacterium having effective cellulose degrading property and capable of generating methane is prepared by the method.

The invention discloses a vaginitis test kit which comprises a reaction device, a sampling test tube, a straw, joint test diluent, joint test chromogenic reagent, joint test stop solution, a user manual and a joint test colourimetric card, wherein a hydrogen peroxide reaction hole, a leukocyte esterase reaction hole, a sialic acid glucoside enzyme reaction hole, a beta- glucuronic acid enzyme reaction hole, a P glucosidase reation hole and a pH value hole; relevant reaction bases are arranged in the reaction holes; reach reaction base comprises a corresponding reaction substrate curing layer, a chromogenic promotional layer and a chemical inert carrier layer. The invention also provides a preparation method of the test kit and the preparation method of a novel chemical carrier. The test kit can be used for distinguishing bacterial vaginosis and vaginitis, and can further identify aerobic / anaerobic bacteria, facultative anaerobic bacteria and other flora in vaginal secretion. The method is simple and quick to operate, has high accuracy, and is applicable to clinical practice, particularly hospital outpatient practice.

The invention discloses application of rhodococcus pyridinovorans to anaerobic fermentation methanol production. An experiment proves that the rhodococcus pyridinovorans HR-1 is added into a raw material for anaerobic fermentation, the gas production rate of biogas is obviously increased, and content of methanol in the biogas is obviously increased, and therefore, the rhodococcus pyridinovorans HR-1 can be applied to the production of the methanol through anaerobic fermentation. The rhodococcus pyridinovorans HR-1 belongs to facultative anaerobic bacteria, and can be survived in the presence of oxygen, and is easy to culture and easy to operate. According to the application of rhodococcus pyridinovorans to anaerobic fermentation methanol production disclosed by the invention, the new application field is widened for the rhodococcus pyridinovorans, and a new microorganism strain with higher adaptability is provided for an anaerobic fermentation methanol-producing system.

The invention relates to a method for screening facultative anaerobic bacteria producing biosurfactant, which comprises the following steps: (1) collecting an oil-polluted environment sample, and storing in an environment of 4 DEG C; (2) taking a small amount of samples, adding the samples into an enrichment medium for culture, inoculating the obtained culture solution into a newly prepared enrichment medium for culture, and continuously transferring and culturing for three times; (3) taking the enrichment culture solution transferred for the third time, and coating the enrichment culture solution on an LB plate for culture to obtain a single colony; (4) inoculating the single colony into a fermentation medium for culture, measuring the surface tension of a culture solution, and selectinga strain with the surface tension of the culture solution lower than 30mN / m; and (5) inoculating the selected strain on a crude oil flat plate, and culturing to obtain the strain with an obvious oil discharge ring, namely the anaerobic strain for producing the biosurfactant. Compared with the prior art, the method has the advantage that facultative anaerobic product surfactant strains can be obtained through quick and efficient screening.

The invention discloses a strain preservation method of anaerobic bacteria. The strain preservation method comprises the steps of firstly inoculating a strain to be preserved into an anaerobic liquidculture medium, executing culturing to enable the strain to grow to a logarithmic phase; transferring the log-phase fresh culture bacterial solution into a strain preservation and freezing storage tube in an anaerobic workstation by using a syringe, adding an anti-freezing agent, oscillating and uniformly mixing the bacterial solution; and finally adding a liquid paraffin-oxygen isolating agent and then executing cryopreservation at the temperature of -80 DEG C. The strain preservation method is suitable for long-term preservation of anaerobic bacteria (facultative anaerobic bacteria / absoluteanaerobic bacteria). By adopting the preservation method , the preservation cost of the strain can be greatly reduced, the strain can be effectively preserved for a long time under an anaerobic condition, and the research and utilization of anaerobic microorganisms are facilitated.

The invention discloses a strain capable of degrading chloramphenicol and synchronously generating electricity and application of the strain. The strain is identified as Raoultella sp. DB-1 according to a 16S rRNA (ribosomal Ribonucleic Acid) sequence, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No. 1.19109. The strain DB-1 of the Raoultella sp. Is a functional strain capable of degrading chloramphenicol and synchronously generating electricity, and the strain DB-1 of the Raoultella sp. Is a functional strain capable of degrading chloramphenicol and synchronously generating electricity. As facultative anaerobic bacteria, the strain is high in growth and reproduction speed, can be grown and reproduced by utilizing multiple carbon sources, and has relatively high adaptive capacity to the environment. The Raoultella sp. DB-1 strain is applied to a microbial fuel cell as an anode catalyst, and can efficiently degrade chloramphenicol and synchronously generate electricity in a bioelectrochemical system.