96 results about "Facultative anaerobic bacteria" patented technology

Animal waste such as fecal material from swine, chicken, turkey, and cattle is converted into useful forms such as fertilizer, other types of soil builders, and even nutrient feed additives. Devices, systems, and methods are provided that allow economical conversion and in many instances, alleviate the production and release of undesirable gases such as sulfide and ammonia. In one embodiment, undesirable anaerobic and facultative anaerobic bacteria are killed at a greater rate than desirable soil compatible aerobic bacteria. The use of low temperature killing diminishes off gassing commonly associated with other techniques, and the use of gas trapping additives such as zeolites provides enhanced soil building qualities such as slow release of nitrogen and slow release of moisture to soil. The methods allow convenient adjustment of composition, allowing conversion of waste into tailored designer fertilizer suited for particular soils. Other embodiments provide other soil building qualities as well as nutrient qualities for feedstock used in animal husbandry such as aquaculture.

The invention relates to the field of wastewater treatment, and relates to a biological degradation method of nitrogen-containing heterocyclic compound wastewater. An oxidation state compound is added in the nitrogen containing heterocyclic compound wastewater, an anaerobic environment is formed by micro aeration, and mixed electron acceptors of dissolved oxygen and the oxidation state compound simultaneously exist in the system; an upflow biological aerated filter is used as a main reaction device, an optimal degradation effect of nitrogen-containing heterocyclic compounds is achieved according to different components and concentrations of the nitrogen-containing heterocyclic compounds, the pH is controlled to 7.0-7.5, the concentration of the dissolved oxygen is controlled to 0.05-0.3 mg / L (water discharged from the upper part of the filter), and the range of the oxidation-reduction potential is controlled to 150-50 mv; under an anaerobic aeration condition, with the nitrogen-containing heterocyclic compounds as electron donors and oxygen and oxygen-containing compounds as electron acceptors, under the combined effects of micro aerobic bacteria, facultative anaerobic bacteria, aerobic / anoxic denitrifying bacteria and the like, the nitrogen-containing heterocyclic compounds are completely degraded into carbon dioxide and water.

Animal waste such as fecal material from swine, chicken, turkey, and cattle is converted into useful forms such as fertilizer, other types of soil builders, and even nutrient feed additives. Devices, systems, and methods are provided that allow economical conversion and in many instances, alleviate the production and release of undesirable gases such as sulfide and ammonia. In one embodiment, undesirable anaerobic and facultative anaerobic bacteria are killed at a greater rate than desirable soil compatible aerobic bacteria. The use of low temperature killing diminishes off gassing commonly associated with other techniques, and the use of gas trapping additives such as zeolites provides enhanced soil building qualities such as slow release of nitrogen and slow release of moisture to soil. The methods allow convenient adjustment of composition, allowing conversion of waste into tailored designer fertilizer suited for particular soils. Other embodiments provide other soil building qualities as well as nutrient qualities for feedstock used in animal husbandry such as aquaculture.

Described herein is a method of treatment of cancer or tumor using a modified bacteria or composition comprising the modified bacteria. In certain embodiments, the method is in combination with other treatment. In certain embodiments, the treatment is chemotherapy, radiation therapy, gene therapy, surgery or a combination thereof. The method makes modified facultative anaerobic bacteria into a conditional obligate anaerobe. The modified bacteria are strictly hypoxia regulated and comprise an essential gene expressing cassette. The vectors of this method comprise the essential gene expressing cassette. Also described herein are therapeutic and prophylactic compositions comprising the modified bacteria. The therapeutic and prophylactic compositions contain a purified form of the modified bacteria, while in certain embodiments, they do not contain other strain of microorganisms. The modified bacteria grow within the solid tumor / cancer, retarding its growth and are rapidly eliminated from normal tissues. The solid tumor / cancer includes breast cancer, liver cancer or neuroblastoma.

The invention discloses a method for preparing composite bacterium, comprising the following steps: inoculating microorganism of zymogenous bacteria, hydrogen-producing acetogenic bacteria and methanogen respectively belonging to facultative anaerobic bacteria and strict anaerobic bacteria which are mixed according to a certain proportion into a culture solution containing cellulose, sealing and keeping standing to culture at a temperature of between 30 to 50 DEG C and pH value of 3 to 10, wherein the culture solution containing cellulose contains CaCO3, 0.3 to 0.7 percent of NaCl, 0.5 to 1 percent of cellulose, 0.1 to 0.4 percent of urea, 0.1 to 0.3 percent of peptone and 0.05 to 0.15 percent of yeast powder, and water is used as a solvent. The composite bacterium having effective cellulose degrading property and capable of generating methane is prepared by the method.

The invention discloses a vaginitis test kit which comprises a reaction device, a sampling test tube, a straw, joint test diluent, joint test chromogenic reagent, joint test stop solution, a user manual and a joint test colourimetric card, wherein a hydrogen peroxide reaction hole, a leukocyte esterase reaction hole, a sialic acid glucoside enzyme reaction hole, a beta- glucuronic acid enzyme reaction hole, a P glucosidase reation hole and a pH value hole; relevant reaction bases are arranged in the reaction holes; reach reaction base comprises a corresponding reaction substrate curing layer,a chromogenic promotional layer and a chemical inert carrier layer. The invention also provides a preparation method of the test kit and the preparation method of a novel chemical carrier. The test kit can be used for distinguishing bacterial vaginosis and vaginitis, and can further identify aerobic / anaerobic bacteria, facultative anaerobic bacteria and other flora in vaginal secretion. The method is simple and quick to operate, has high accuracy, and is applicable to clinical practice, particularly hospital outpatient practice.

The invention is about a kind of magnetotactic bacterium which is separated from the mud sample in the iron-affluent watershed. The bacterium is preserved in the Chinese typical culture collection center. The preserving code is CCTCC NO:M205105 while the classified name is Magnetotactic bacterium MB-TZ. The bacterium is facultative anoxybiontic bacterium and the state of the thallus is circular staff. A thallus can produce 3-20 circular magnetic globules which are mainly comprised by iron, calcium, fosforus, oxygen and magnesium. The bacterium has many advantages such as short producing cycle, easy to cultivate and easy to be produced in factories. During the extracting and purifying of the magnetic globules, the invention adopts the treating method of repeating freeze thawing combining with supersonics, so it can dissolve the thallus more thoroughly and increase the yield of magnetic globules.

The invention discloses a method for biologically treating industrial circulating water and stabilizing the quality thereof. The method is characterized by comprising a, separately breeding bacterial strains; b, performing acclimatization for the bacterial strains; c, culturing and preparing the bacteria; d, culturing the bacteria in an industrial expanding manner to produce medicinal products; and e, directly adding the produced medicinal products into a circulating water pond. The method has the advantages that the quality of the industrial circulating cooling water is stabilized by the aid of the biotechnology and method, in a circulating water system, currently preferred chemical corrosion and scale inhibitors, bactericides and other chemical agents are replaced by microbial agents; and the microbial agents are produced by a special process, and aerobic bacteria, anaerobic bacteria and facultative anaerobic bacteria which have biological reaction functions are optimized, are subjected to acclimatization and then are cultured in an industrial expanding manner to obtain the microbial agents (medicament or microbiological products in short).

The invention discloses a production method for lipid through synchronous mixed culture of microbes. The method comprises the following steps: (1) culturing facultative anaerobic bacteria seed liquid; (2) culturing autotrophic microalgae seed liquid; and (3) mixing the facultative anaerobic bacteria seed liquid and the autotrophic microalgae seed liquid and carrying out synchronous mixed culture in an illuminated bioreactor, wherein during synchronous mixed culture, an organic carbon source needed in growth and metabolism of the facultative anaerobic bacteria is supplemented. The method provided by the invention has the advantages of increased harvest yield of autotrophic microalgae, an improved utilization rate of an inorganic carbon source (CO2), increased content of microbial lipid, a simplified culture apparatus and the like, and exerts small influence on the fermentation process of the facultative anaerobic bacteria, thereby realizing synchronous culture of the facultative anaerobic bacteria and the autotrophic microalgae.

The invention discloses a production method for lipid through coupled culture of microbes. The method comprises the following steps: (1) culturing autotrophic microalgae seed liquid and carrying out enlarged culture until the OD value of biomass is 5.0 to 10.0, wherein the pH value of microalgae seed liquid having undergone enlarged culture is 9 to 12; (2) culturing facultative anaerobic bacteria seed liquid and carrying out fed-batch fermentation; and (3) during the process of fed-batch fermentation of facultative anaerobic bacteria, controlling the pH value in the fermentation process of the facultative anaerobic bacteria to be in a range of 6 to 9 in an illuminated bioreactor by using the prepared microalgae seed liquid having undergone enlarged culture in step (1) as an alkali neutralizing agent for bacterial fermentation and carrying out coupled culture of the facultative anaerobic bacteria and autotrophic microalgae. The method provided by the invention has the advantages of increased harvest yield of the autotrophic microalgae, an improved utilization rate of an inorganic carbon source, increased content of microbial lipid, a simplified culture apparatus and the like.

A soil-based disposal trench that has failed from a biomat slime is recovered by adding a facultative anaerobic bacteria to an effluent that flows into the disposal trench. The facultative anaerobic bacteria consumes the biomat slime and, in one embodiment, supplants a strictly aerobic bacteria in the soil that converts nitrites to nitrates. As a result, the facultative anaerobic bacteria interrupt the conventional process of nitrification, changing the nitrites to nitrogen gas.

The invention belongs to the field of feed packaging, in particular to a bagged fermented feed package for livestock and poultry without antibiotics and high live bacteria. Bagged fermented feed package for livestock and poultry without antibiotics and high live bacteria. The fermented feed package is composed of a packaging bag and a feed base material packed in the packaging bag to inoculate the bacteria. A mixed bacterial solution with a bacterial count greater than 108cfu / g, the mixed bacterial solution is composed of aerobic bacteria and facultative anaerobic bacteria; the packaging bag is equipped with a one-way air outlet valve device. The fermented feed prepared by using the fermented feed package has comprehensive nutrition of the mixture, is fermented, has biological activity, does not remain, and does not produce drug resistance.

The invention discloses a green desert ground surface windproof structure. A windproof layer covers the desert ground surface, and the windproof layer is obtained through microorganism seepage-proofing and strengthening fluid treatment; the content of sandy soil in desert with the particle size being 0.075-60 mm is larger than 50%, and microorganisms in strengthening fluid are aerobiotic type bacteria; or the content of sandy soil in desert with the particle size being smaller than or equal to 0.075 is larger than or equal to 50%, the microorganisms in strengthening fluid are facultative anaerobic bacteria, and the windproof layer cannot be blown and scattered at the maximum wind speed of 20 m / s. The microorganism reaction process depended by the green desert ground surface windproof structure is the process existing in the soil ecological environment, and the environment cannot be polluted; a microorganism-plant combined sand treatment technology is adopted, and the survival rate is higher compared with pure planting of plants; the green desert ground surface windproof structure mainly depends on the biocement technology, the effect is high in speed compared with biological crust generated by utilizing other bacteria, fungi, blue-green algae and the like, and basically no requirement exists for the environment.

The invention discloses an oxygen-rich suspending chain type microporous aerating device. The device comprises an air compressor, a main oxygen preparing machine, a gas inlet pipe, a high tower aerating pond, a three-phase separator and a water absorbing pump; the air compressor is connected with the main oxygen preparing machine; the oxygen preparing machine is used to prepare oxygen by the PSA (Pressure Swing Adsorption) method; one end of the gas inlet pipe is connected with the main oxygen preparing machine and while the other end of the gas inlet pipe extends through the aerating pond; pure oxygen prepared by the main oxygen preparing machine enters the gas inlet pipe; a stop valve and a filter are arranged on the gas inlet pipe; a condensate water draining valve is arranged at the tail end of the gas inlet pipe; at least two gas inlet hoses which are connected with an aerating pipe are connected to the gas inlet pipe; two ends of the aerating pipes are sealed; the aerating pipe is also equipped with falling parts which are symmetrically arranged at two sides of the aerating pipe; the falling parts are suspended on the gas inlet pipe through a suspending chain. According to the oxygen-rich suspending chain type microporous aerating device, blocking is greatly avoided, the aerating effect is great, the oxygenation capacity is high, the generation of some anaerobic bacteria (facultative anaerobic bacteria) can be effectively inhibited, and the strength of ozone is also reduced.

The invention relates to the field of biology and provides a method for performing mixed fermentation on lactobacillus acidophilus and clostridium butyricum. The method comprises the following steps:inoculating the lactobacillus acidophilus on an unsterilized solid culture medium to perform fermentation, and then inoculating clostridium butyricum liquid to perform fermentation. The synergistic inhibition effect on infectious microbes by the lactobacillus acidophilus and the clostridium butyricum can be utilized, the growth of the infectious microbes can be effectively inhibited and the infectious microbes in the production process can be effectively controlled; furthermore, under the condition of not sterilizing the raw materials, the lactobacillus acidophilus and the clostridium butyricum are fermented, so that the cost is reduced and the practicability is high. In addition, the invention also provides fermentation equipment. The fermentation equipment can be applied to solid fermentation production of anaerobic bacteria or facultative anaerobic bacteria such as the clostridium butyricum and the lactobacillus acidophilus; and the fermentation equipment discharges volatile metabolic products such as hydrogen and carbon dioxide to the external air through a gas outlet pipe, relieves metabolic inhibition, and can release viable bacteria to improve the growth and reproduction capacity and increase the number of the fermented viable bacteria.

The present invention provides compositions of therapeutic agents and methods of use for reducing and / or treating gastrointestinal inflammation. In particular aspects, the tungstate salts described herein and pharmaceutical compositions thereof inhibit the activity of one or a plurality of anaerobic respiratory enzymes in facultative anaerobic bacteria such as, for example, Enterobacteriaceae, that can exacerbate inflammation. In particular embodiments, the present invention provides compositions of therapeutic agents for treating gastrointestinal inflammation, as well as methods for treating or preventing gut microbial imbalance due to an increase in the abundance of intestinal Enterobacteriaceae.

The invention discloses an enclosed rapid culture method of microalgae. The method includes the steps of: (1) cultivating a facultative anaerobic bacteria seed liquid; (2) cultivating an autotrophic microalgae seed liquid; and (3) inoculating the facultative anaerobic bacteria seed liquid and the autotrophic microalgae seed liquid into a closed photobioreactor with a built-in membrane separation device to carry out semi-mixing culture, fermenting the anaerobic bacteria in the membrane separation device, and subjecting the autotrophic microalgae to photosynthesis growth in the photobioreactor outside the membrane separation device, wherein the medium composition and culture conditions are the same in the two reactors, and supplementing organic carbon source required by the growth and metabolism of the facultative anaerobic bacteria in the culture process. The photobioreactor also comprises a guide shell, the membrane separation device is arranged in the guide shell, and a reflective membrane is pasted on the outer surface of the guide shell. The method has the advantages of increasing the yield of autotrophic microalgae, the utilization rate of an inorganic carbon source (CO2), and oil content of the microorganisms, and simplifying the culture device.

The invention relates to the technical field of biology, in particular to a nano-selenium-organic selenium nutrient solution and a preparation method and application thereof. The preparation method of the nano-selenium-organic selenium nutrient solution comprises the following steps that Providenciaretgeri is inoculated into a culture solution containing inorganic selenium for being fermented for 16 to 150 hours, so that the nano-selenium-organic selenium nutrient solution is obtained; and the preservation number of the providenciaretgeri is CCTCC M 2020956. The providenciaretgeri is facultative anaerobic bacteria, during large-scale fermentation culture, the requirement of strict aerobic bacteria on dissolved oxygen of the culture solution is avoided, and the requirement on fermentation equipment is relatively low; and meanwhile, the fermentation control difficulty is further reduced, and the fermentation process is easier to amplify.

The invention provides cold hydrogel and application thereof, the cold hydrogel comprises xanthan gum, guar gum and sodium polyacrylate, and the mass ratio of xanthan gum to guar gum to sodium polyacrylate is (1-2) : (1-2) : 1. The invention also provides a microbiological detection culture medium. The microbiological detection culture medium comprises the cold hydrogel. The xanthan gum, guar gum and sodium polyacrylate are reasonably proportioned, so that the cold hydrogel has excellent water absorptivity, water retention property and gelation speed; a microorganism detection culture medium is better in transparency and high in gel strength, and facilitates observation, counting and picking of bacterial colonies. A microbiological detection kit, prepared by fixing the microbiological detection culture medium powder on the inner bottom wall of a culture dish through an adhesive sticker, is convenient to use, can be applied to detection of aerobic bacteria and facultative anaerobic bacteria, and is simple and efficient in preparation method and extremely high in application value.

A hydrogen producing method by culturing a microorganism having a hydrogenase gene, in which a lactic acid biogenetic path and a succinic acid biogenetic path in anaerobic metabolic paths are inactivated, under an anaerobic condition in the presence of organic substrate. The microorganism may further have a formate dehydrogenase gene. The microorganism may be Escherichia coli which is a facultative anaerobic bacterium.

The invention discloses application of rhodococcus pyridinovorans to anaerobic fermentation methanol production. An experiment proves that the rhodococcus pyridinovorans HR-1 is added into a raw material for anaerobic fermentation, the gas production rate of biogas is obviously increased, and content of methanol in the biogas is obviously increased, and therefore, the rhodococcus pyridinovorans HR-1 can be applied to the production of the methanol through anaerobic fermentation. The rhodococcus pyridinovorans HR-1 belongs to facultative anaerobic bacteria, and can be survived in the presence of oxygen, and is easy to culture and easy to operate. According to the application of rhodococcus pyridinovorans to anaerobic fermentation methanol production disclosed by the invention, the new application field is widened for the rhodococcus pyridinovorans, and a new microorganism strain with higher adaptability is provided for an anaerobic fermentation methanol-producing system.

The invention discloses a method for improving bioavailability of apple juice polyphenol, and belongs to the technical field of food fermentation. The technical scheme of the invention comprises the following steps of: activating lactobacillus rhamnosus zrx01, domesticating strains, and fermenting apple juice. Compared with the prior art, the method has the beneficial effects that (1) the lactobacillus rhamnosus belongs to anaerobic bacteria, is not easy to grow, and the apple juice can be rancid if the fermentation is not good, while the lactobacillus rhamnosus zrx01 in the invention is facultative anaerobic bacteria, and is easier to reproduce and grow in the apple juice; (2) the adaptability of the strains is increased by continuously domesticating the strains; and (3) the apple juice is fermented by the lactobacillus rhamnosus, so that the apple polyphenol can be converted into small molecules which are easy to absorb, and the bioavailability of the polyphenol in vivo is improved.

The invention relates to a method for screening facultative anaerobic bacteria producing biosurfactant, which comprises the following steps: (1) collecting an oil-polluted environment sample, and storing in an environment of 4 DEG C; (2) taking a small amount of samples, adding the samples into an enrichment medium for culture, inoculating the obtained culture solution into a newly prepared enrichment medium for culture, and continuously transferring and culturing for three times; (3) taking the enrichment culture solution transferred for the third time, and coating the enrichment culture solution on an LB plate for culture to obtain a single colony; (4) inoculating the single colony into a fermentation medium for culture, measuring the surface tension of a culture solution, and selectinga strain with the surface tension of the culture solution lower than 30mN / m; and (5) inoculating the selected strain on a crude oil flat plate, and culturing to obtain the strain with an obvious oil discharge ring, namely the anaerobic strain for producing the biosurfactant. Compared with the prior art, the method has the advantage that facultative anaerobic product surfactant strains can be obtained through quick and efficient screening.

The invention discloses a strain preservation method of anaerobic bacteria. The strain preservation method comprises the steps of firstly inoculating a strain to be preserved into an anaerobic liquidculture medium, executing culturing to enable the strain to grow to a logarithmic phase; transferring the log-phase fresh culture bacterial solution into a strain preservation and freezing storage tube in an anaerobic workstation by using a syringe, adding an anti-freezing agent, oscillating and uniformly mixing the bacterial solution; and finally adding a liquid paraffin-oxygen isolating agent and then executing cryopreservation at the temperature of -80 DEG C. The strain preservation method is suitable for long-term preservation of anaerobic bacteria (facultative anaerobic bacteria / absoluteanaerobic bacteria). By adopting the preservation method , the preservation cost of the strain can be greatly reduced, the strain can be effectively preserved for a long time under an anaerobic condition, and the research and utilization of anaerobic microorganisms are facilitated.

The invention discloses a strain capable of degrading chloramphenicol and synchronously generating electricity and application of the strain. The strain is identified as Raoultella sp. DB-1 according to a 16S rRNA (ribosomal Ribonucleic Acid) sequence, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No. 1.19109. The strain DB-1 of the Raoultella sp. Is a functional strain capable of degrading chloramphenicol and synchronously generating electricity, and the strain DB-1 of the Raoultella sp. Is a functional strain capable of degrading chloramphenicol and synchronously generating electricity. As facultative anaerobic bacteria, the strain is high in growth and reproduction speed, can be grown and reproduced by utilizing multiple carbon sources, and has relatively high adaptive capacity to the environment. The Raoultella sp. DB-1 strain is applied to a microbial fuel cell as an anode catalyst, and can efficiently degrade chloramphenicol and synchronously generate electricity in a bioelectrochemical system.

The invention discloses a preparation method of facultative anaerobic bacteria filler for in-situ purification of domestic sewage. By utilizing the method, facultative anaerobic microorganism bacteriacapable of treating domestic sewage can be constructed, the bacteria are loaded on a carrier which is large in surface area, porous and rough in surface, efficient loading of microorganism strains isachieved, and the facultative anaerobic microorganism filler capable of treating domestic sewage is obtained through a freeze drying technology. The invention has the advantages that the facultativeanaerobic microorganisms for treating the domestic sewage can be rapidly immobilized on the carrier by utilizing the technology, and the effective viable count is higher than 1010cfu / g. The facultative anaerobic bacteria filler constructed by the method has the characteristics of high porosity, large specific surface area, rich functional groups (hydroxyl and amino), high effective viable count and the like. By utilizing the filler constructed by the invention, the facultative anaerobic treatment efficiency can be remarkably improved, and pollutant removal is accelerated.