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Application of rhodococcus pyridinovorans to anaerobic fermentation methanol production

A technology of Rhodococcus pyridinephilus and anaerobic fermentation, which is applied in the application field of Rhodococcus pyridinephilus in anaerobic fermentation of methane, can solve problems that have not yet been discovered, and achieve improved biogas production, a wide spectrum of electron donors, and easy The effect of cultivation

Active Publication Date: 2019-05-28
GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After searching the literature and patents, no reports have been found on the ability of Rhodococcus pyridineophilus to promote anaerobic fermentation to produce methane

Method used

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  • Application of rhodococcus pyridinovorans to anaerobic fermentation methanol production
  • Application of rhodococcus pyridinovorans to anaerobic fermentation methanol production
  • Application of rhodococcus pyridinovorans to anaerobic fermentation methanol production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Verification of the performance of Rhodococcus pyridineophilus in promoting anaerobic fermentation to produce methane

[0023] (1) Construct a small-scale test system of anaerobic fermentation producing methane:

[0024] Such as figure 1 As shown, the small test system for producing methane by anaerobic fermentation of the present invention includes an anaerobic fermentation reaction device 1 , a constant temperature control device 2 , a gas collection device 3 and a measuring device 4 . The anaerobic fermentation reaction device 1 comprises the first conical flask 12 sealed with the first rubber stopper 11, the first conical flask 12 is a 2000mL conical flask, the first rubber stopper 11 is provided with an exhaust pipe 13 and a sample feeding pipe 14, and The sample feeding end of the sample tube 14 is provided with a first valve 15 . The constant temperature control device 2 is a constant temperature water tank, and the anaerobic fermentation reaction de...

Embodiment 2

[0034] Concrete operation process is identical with embodiment 1, and difference is:

[0035] The raw material in the small-scale test system for anaerobic fermentation methane production is chicken manure. The original biogas production of anaerobic fermentation of chicken manure was 320-350mL / g·VS, and the methane content in the generated biogas was about 57.3%. After the inoculum of Rhodococcus pyridineophilus, the gas production of biogas increased to 420±30mL / g VS, while the methane content in the biogas increased to 73.1%, as image 3 .

Embodiment 3

[0037] Concrete operation process is identical with embodiment 1, and difference is:

[0038] The raw material in the small test system of anaerobic fermentation methane production is kitchen waste. The original biogas production of anaerobic fermentation of kitchen waste was 230-250mL / g·VS, and the methane content in the generated biogas was about 48.3%. After a good inoculum containing Rhodococcus pyridineophilus, the gas production of biogas increased to 320±30mL / g VS, and the methane content in biogas increased to 67.3%, as Figure 4 .

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Abstract

The invention discloses application of rhodococcus pyridinovorans to anaerobic fermentation methanol production. An experiment proves that the rhodococcus pyridinovorans HR-1 is added into a raw material for anaerobic fermentation, the gas production rate of biogas is obviously increased, and content of methanol in the biogas is obviously increased, and therefore, the rhodococcus pyridinovorans HR-1 can be applied to the production of the methanol through anaerobic fermentation. The rhodococcus pyridinovorans HR-1 belongs to facultative anaerobic bacteria, and can be survived in the presence of oxygen, and is easy to culture and easy to operate. According to the application of rhodococcus pyridinovorans to anaerobic fermentation methanol production disclosed by the invention, the new application field is widened for the rhodococcus pyridinovorans, and a new microorganism strain with higher adaptability is provided for an anaerobic fermentation methanol-producing system.

Description

Technical field: [0001] The invention belongs to the field of microbial system classification and environmental biotechnology, and in particular relates to the application of rhodococcus pyridineophilus in anaerobic fermentation for methane production. Background technique: [0002] Anaerobic fermentation mainly refers to the process of decomposing organic matter under anaerobic conditions to produce methane and carbon dioxide. The performance of methane is one of the current research hotspots. [0003] Previous studies have shown that adding iron-based additives, graphene, and extracellular polymers to the anaerobic fermentation system can effectively promote the anaerobic fermentation efficiency of the system and increase the methane yield. However, the addition of such substances generally has problems such as increased operating costs and affects the utilization of biogas slurry and biogas residues. However, studies have shown that methanogens play a key role in the an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P5/02C02F11/04C12R1/01
CPCY02E50/30
Inventor 袁浩然邓丽芳李德念王鲁丰钱鑫郑涛
Owner GUANGZHOU INST OF ENERGY CONVERSION - CHINESE ACAD OF SCI
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